Immunohistochemical and molecular expression of laminin-332 gamma-2 chain in canine mammary tumors

• Autor(a) principal: Zuccari, D. A. P. C.
• Data de Publicação: 2011
• Outros Autores: Castro, R., Jardim, B. V. [UNESP], Mancini, U. M., Polachini, G. M.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1590/S0102-09352011000100005; http://hdl.handle.net/11449/40187
• topic: dog; adhesion molecule; cancer; western blot; RT-PCR

Forty-eight cases of canine mammary cancer were investigated to evaluate the immunohistochemical distribution of the gamma 2 chain of laminin-332. Tumor cells were compared to a pool of normal mammary tissues using quantitative RT-PCR. The western blot was performed in eight tumor samples as complementary test to evaluate protein integrity. Immunohistochemistry experiments showed negative, focal, and weak expression of laminin-332 gamma 2 in tumors with the worst prognosis. Quantitative PCR revealed downregulation of the gene in 27 (56.2%) of the animals. Out of the 16 dogs with gamma 2 chain overexpression, seven were still alive. The western blot results showed bands generation of 36, 50, and 98kDa, suggesting degradation of laminin-332 gamma 2 in malignant tumors. The results suggest that, in the future, low expression and/or degradation of laminin-332 gamma 2 chain in canine mammary tumors may be used as an indicator of malignant potential. However, further studies are necessary to corroborate these results.