Antioxidant Effect of Melatonin on the Functional Activity of Colostral Phagocytes in Diabetic Women
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0056915 http://hdl.handle.net/11449/74618 |
Resumo: | Melatonin is involved in a number of physiological and oxidative processes, including functional regulation in human milk. The present study investigated the mechanisms of action of melatonin and its effects on the functional activity of colostral phagocytes in diabetic women. Colostrum samples were collected from normoglycemic (N = 38) and diabetic (N = 38) women. We determined melatonin concentration, superoxide release, bactericidal activity and intracellular Ca2+ release by colostral phagocytes treated or not with 8-(Diethylamino) octyl-3,4,5-trimethoxybenzoate hydrochloride (TMB-8) and incubated with melatonin and its precursor (N-acetyl-serotonin-NAS), antagonist (luzindole) and agonist (chloromelatonin-CMLT). Melatonin concentration was higher in colostrum samples from hyperglycemic than normoglycemic mothers. Melatonin stimulated superoxide release by colostral phagocytes from normoglycemic but not hyperglycemic women. NAS increased superoxide, irrespective of glycemic status, whereas CMTL increased superoxide only in cells from the normoglycemic group. Phagocytic activity in colostrum increased significantly in the presence of melatonin, NAS and CMLT, irrespective of glycemic status. The bactericidal activity of colostral phagocytes against enterophatogenic Escherichia coli (EPEC) increased in the presence of melatonin or NAS in the normoglycemic group, but not in the hyperglycemic group. Luzindole blocked melatonin action on colostrum phagocytes. Phagocytes from the normoglycemic group treated with melatonin exhibited an increase in intracellular Ca2+ release. Phagocytes treated with TMB-8 (intracellular Ca2+ inhibitor) decreased superoxide, bactericidal activity and intracellular Ca2+ release in both groups. The results obtained suggest an interactive effect of glucose metabolism and melatonin on colostral phagocytes. In colostral phagocytes from normoglycemic mothers, melatonin likely increases the ability of colostrum to protect against EPEC and other infections. In diabetic mothers, because maternal hyperglycemia modifies the functional activity of colostrum phagocytes, melatonin effects are likely limited to anti-inflammatory processes, with low superoxide release and bactericidal activity. © 2013 Morceli et al. |
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Antioxidant Effect of Melatonin on the Functional Activity of Colostral Phagocytes in Diabetic Women3,4,5 trimethoxybenzoic acid 8 (diethylamino)octyl esterchloromelatoninluzindolemelatoninn acetylserotoninsuperoxideunclassified drugadultbactericidal activitycalcium transportcell functionclinical articlecolostrumcontrolled studyenteropathogenic Escherichia colifemaleglucose metabolismhumanhuman cellmaternal diabetes mellitusnonhumanphagocytephagocytosisAdolescentAdultAntioxidantsCalciumColostrumCross-Sectional StudiesEscherichia coliFemaleHumansHyperglycemiaMelatoninMiddle AgedPhagocytesPhagocytosisPregnancySuperoxidesYoung AdultMelatonin is involved in a number of physiological and oxidative processes, including functional regulation in human milk. The present study investigated the mechanisms of action of melatonin and its effects on the functional activity of colostral phagocytes in diabetic women. Colostrum samples were collected from normoglycemic (N = 38) and diabetic (N = 38) women. We determined melatonin concentration, superoxide release, bactericidal activity and intracellular Ca2+ release by colostral phagocytes treated or not with 8-(Diethylamino) octyl-3,4,5-trimethoxybenzoate hydrochloride (TMB-8) and incubated with melatonin and its precursor (N-acetyl-serotonin-NAS), antagonist (luzindole) and agonist (chloromelatonin-CMLT). Melatonin concentration was higher in colostrum samples from hyperglycemic than normoglycemic mothers. Melatonin stimulated superoxide release by colostral phagocytes from normoglycemic but not hyperglycemic women. NAS increased superoxide, irrespective of glycemic status, whereas CMTL increased superoxide only in cells from the normoglycemic group. Phagocytic activity in colostrum increased significantly in the presence of melatonin, NAS and CMLT, irrespective of glycemic status. The bactericidal activity of colostral phagocytes against enterophatogenic Escherichia coli (EPEC) increased in the presence of melatonin or NAS in the normoglycemic group, but not in the hyperglycemic group. Luzindole blocked melatonin action on colostrum phagocytes. Phagocytes from the normoglycemic group treated with melatonin exhibited an increase in intracellular Ca2+ release. Phagocytes treated with TMB-8 (intracellular Ca2+ inhibitor) decreased superoxide, bactericidal activity and intracellular Ca2+ release in both groups. The results obtained suggest an interactive effect of glucose metabolism and melatonin on colostral phagocytes. In colostral phagocytes from normoglycemic mothers, melatonin likely increases the ability of colostrum to protect against EPEC and other infections. In diabetic mothers, because maternal hyperglycemia modifies the functional activity of colostrum phagocytes, melatonin effects are likely limited to anti-inflammatory processes, with low superoxide release and bactericidal activity. © 2013 Morceli et al.Post Graduate Program in Gynecology, Obstetrics and Mastology of Botucatu Medical School São Paulo State University/Unesp, Botucatu, São PauloInstitute of Biological and Health Science Federal University of Mato Grosso, Barra do Garças, Mato GrossoPost Graduate Program in Gynecology, Obstetrics and Mastology of Botucatu Medical School São Paulo State University/Unesp, Botucatu, São PauloUniversidade Estadual Paulista (Unesp)Federal University of Mato GrossoMorceli, Gliciane [UNESP]Honorio-França, Adenilda C.Fagundes, Danny L. G. [UNESP]Calderon, Iracema de Mattos Paranhos [UNESP]França, Eduardo L.2014-05-27T11:28:31Z2014-05-27T11:28:31Z2013-02-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0056915PLoS ONE, v. 8, n. 2, 2013.1932-6203http://hdl.handle.net/11449/7461810.1371/journal.pone.0056915WOS:0003151842001842-s2.0-848742595192-s2.0-84874259519.pdf0679387622604743Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPLOS ONE2.7661,164info:eu-repo/semantics/openAccess2023-11-08T06:12:03Zoai:repositorio.unesp.br:11449/74618Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-08T06:12:03Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antioxidant Effect of Melatonin on the Functional Activity of Colostral Phagocytes in Diabetic Women |
title |
Antioxidant Effect of Melatonin on the Functional Activity of Colostral Phagocytes in Diabetic Women |
spellingShingle |
Antioxidant Effect of Melatonin on the Functional Activity of Colostral Phagocytes in Diabetic Women Morceli, Gliciane [UNESP] 3,4,5 trimethoxybenzoic acid 8 (diethylamino)octyl ester chloromelatonin luzindole melatonin n acetylserotonin superoxide unclassified drug adult bactericidal activity calcium transport cell function clinical article colostrum controlled study enteropathogenic Escherichia coli female glucose metabolism human human cell maternal diabetes mellitus nonhuman phagocyte phagocytosis Adolescent Adult Antioxidants Calcium Colostrum Cross-Sectional Studies Escherichia coli Female Humans Hyperglycemia Melatonin Middle Aged Phagocytes Phagocytosis Pregnancy Superoxides Young Adult |
title_short |
Antioxidant Effect of Melatonin on the Functional Activity of Colostral Phagocytes in Diabetic Women |
title_full |
Antioxidant Effect of Melatonin on the Functional Activity of Colostral Phagocytes in Diabetic Women |
title_fullStr |
Antioxidant Effect of Melatonin on the Functional Activity of Colostral Phagocytes in Diabetic Women |
title_full_unstemmed |
Antioxidant Effect of Melatonin on the Functional Activity of Colostral Phagocytes in Diabetic Women |
title_sort |
Antioxidant Effect of Melatonin on the Functional Activity of Colostral Phagocytes in Diabetic Women |
author |
Morceli, Gliciane [UNESP] |
author_facet |
Morceli, Gliciane [UNESP] Honorio-França, Adenilda C. Fagundes, Danny L. G. [UNESP] Calderon, Iracema de Mattos Paranhos [UNESP] França, Eduardo L. |
author_role |
author |
author2 |
Honorio-França, Adenilda C. Fagundes, Danny L. G. [UNESP] Calderon, Iracema de Mattos Paranhos [UNESP] França, Eduardo L. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Federal University of Mato Grosso |
dc.contributor.author.fl_str_mv |
Morceli, Gliciane [UNESP] Honorio-França, Adenilda C. Fagundes, Danny L. G. [UNESP] Calderon, Iracema de Mattos Paranhos [UNESP] França, Eduardo L. |
dc.subject.por.fl_str_mv |
3,4,5 trimethoxybenzoic acid 8 (diethylamino)octyl ester chloromelatonin luzindole melatonin n acetylserotonin superoxide unclassified drug adult bactericidal activity calcium transport cell function clinical article colostrum controlled study enteropathogenic Escherichia coli female glucose metabolism human human cell maternal diabetes mellitus nonhuman phagocyte phagocytosis Adolescent Adult Antioxidants Calcium Colostrum Cross-Sectional Studies Escherichia coli Female Humans Hyperglycemia Melatonin Middle Aged Phagocytes Phagocytosis Pregnancy Superoxides Young Adult |
topic |
3,4,5 trimethoxybenzoic acid 8 (diethylamino)octyl ester chloromelatonin luzindole melatonin n acetylserotonin superoxide unclassified drug adult bactericidal activity calcium transport cell function clinical article colostrum controlled study enteropathogenic Escherichia coli female glucose metabolism human human cell maternal diabetes mellitus nonhuman phagocyte phagocytosis Adolescent Adult Antioxidants Calcium Colostrum Cross-Sectional Studies Escherichia coli Female Humans Hyperglycemia Melatonin Middle Aged Phagocytes Phagocytosis Pregnancy Superoxides Young Adult |
description |
Melatonin is involved in a number of physiological and oxidative processes, including functional regulation in human milk. The present study investigated the mechanisms of action of melatonin and its effects on the functional activity of colostral phagocytes in diabetic women. Colostrum samples were collected from normoglycemic (N = 38) and diabetic (N = 38) women. We determined melatonin concentration, superoxide release, bactericidal activity and intracellular Ca2+ release by colostral phagocytes treated or not with 8-(Diethylamino) octyl-3,4,5-trimethoxybenzoate hydrochloride (TMB-8) and incubated with melatonin and its precursor (N-acetyl-serotonin-NAS), antagonist (luzindole) and agonist (chloromelatonin-CMLT). Melatonin concentration was higher in colostrum samples from hyperglycemic than normoglycemic mothers. Melatonin stimulated superoxide release by colostral phagocytes from normoglycemic but not hyperglycemic women. NAS increased superoxide, irrespective of glycemic status, whereas CMTL increased superoxide only in cells from the normoglycemic group. Phagocytic activity in colostrum increased significantly in the presence of melatonin, NAS and CMLT, irrespective of glycemic status. The bactericidal activity of colostral phagocytes against enterophatogenic Escherichia coli (EPEC) increased in the presence of melatonin or NAS in the normoglycemic group, but not in the hyperglycemic group. Luzindole blocked melatonin action on colostrum phagocytes. Phagocytes from the normoglycemic group treated with melatonin exhibited an increase in intracellular Ca2+ release. Phagocytes treated with TMB-8 (intracellular Ca2+ inhibitor) decreased superoxide, bactericidal activity and intracellular Ca2+ release in both groups. The results obtained suggest an interactive effect of glucose metabolism and melatonin on colostral phagocytes. In colostral phagocytes from normoglycemic mothers, melatonin likely increases the ability of colostrum to protect against EPEC and other infections. In diabetic mothers, because maternal hyperglycemia modifies the functional activity of colostrum phagocytes, melatonin effects are likely limited to anti-inflammatory processes, with low superoxide release and bactericidal activity. © 2013 Morceli et al. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-02-20 2014-05-27T11:28:31Z 2014-05-27T11:28:31Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0056915 PLoS ONE, v. 8, n. 2, 2013. 1932-6203 http://hdl.handle.net/11449/74618 10.1371/journal.pone.0056915 WOS:000315184200184 2-s2.0-84874259519 2-s2.0-84874259519.pdf 0679387622604743 |
url |
http://dx.doi.org/10.1371/journal.pone.0056915 http://hdl.handle.net/11449/74618 |
identifier_str_mv |
PLoS ONE, v. 8, n. 2, 2013. 1932-6203 10.1371/journal.pone.0056915 WOS:000315184200184 2-s2.0-84874259519 2-s2.0-84874259519.pdf 0679387622604743 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
PLOS ONE 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1799964861797498880 |