Salivary glands in workers of Ruptitermes spp. (Blattaria, Isoptera, Termitidae, Apicotermitinae): a morphological and preoteomic approach
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s00441-021-03469-w http://hdl.handle.net/11449/207710 |
Resumo: | Salivary glands are omnipresent in termites and occur in all developmental stages and castes. They function to produce, store, and secrete compounds, ranging from a feeding function to defensive mechanisms. Here, we provide a complete morphological overview of the salivary glands in the soldierless species Ruptitermes reconditus and R. xanthochiton, and the first proteomic profile of the salivary glands in a Neotropical Apicotermitinae representative, R. reconditus. Salivary glands from both species were composed of several acini, roughly spherical structures composed of two types of central cells (type I and II) and peripheral parietal cells, as well as transporting ducts and two salivary reservoirs. Central cells were richly supplied with electron-lucent secretory vesicles and rough endoplasmic reticulum, a feature of protein-secreting cells. Parietal cells of Ruptitermes spp. had conspicuous characteristics such as electron-lucent secretory vesicles surrounded by mitochondria and well-developed microvilli. Moreover, different individuals showed variation in the secretory cycle of salivary acini, which may be related to polyethism. Ultrastructural analysis evidenced a high synthesis of secretion and also the occurrence of lysosomes and autophagic structures in central cells. Proteomic analysis of the salivary glands revealed 483 proteins divided into functional groups, highlighting toxins/defensins and compounds related to alarm communication and colony asepsis. Soldierless termites are quite successful, especially due to morphological adaptations of the workers, including unknown modifications of exocrine glands. Thus, according to our morphological and proteomic findings, we discuss the potential roles of the salivary gland secretion in different social aspects of the sampled species. |
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Repositório Institucional da UNESP |
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Salivary glands in workers of Ruptitermes spp. (Blattaria, Isoptera, Termitidae, Apicotermitinae): a morphological and preoteomic approachCentral cellsLCMS-based proteomicsParietal cellsSecretionToxin-like proteinsSalivary glands are omnipresent in termites and occur in all developmental stages and castes. They function to produce, store, and secrete compounds, ranging from a feeding function to defensive mechanisms. Here, we provide a complete morphological overview of the salivary glands in the soldierless species Ruptitermes reconditus and R. xanthochiton, and the first proteomic profile of the salivary glands in a Neotropical Apicotermitinae representative, R. reconditus. Salivary glands from both species were composed of several acini, roughly spherical structures composed of two types of central cells (type I and II) and peripheral parietal cells, as well as transporting ducts and two salivary reservoirs. Central cells were richly supplied with electron-lucent secretory vesicles and rough endoplasmic reticulum, a feature of protein-secreting cells. Parietal cells of Ruptitermes spp. had conspicuous characteristics such as electron-lucent secretory vesicles surrounded by mitochondria and well-developed microvilli. Moreover, different individuals showed variation in the secretory cycle of salivary acini, which may be related to polyethism. Ultrastructural analysis evidenced a high synthesis of secretion and also the occurrence of lysosomes and autophagic structures in central cells. Proteomic analysis of the salivary glands revealed 483 proteins divided into functional groups, highlighting toxins/defensins and compounds related to alarm communication and colony asepsis. Soldierless termites are quite successful, especially due to morphological adaptations of the workers, including unknown modifications of exocrine glands. Thus, according to our morphological and proteomic findings, we discuss the potential roles of the salivary gland secretion in different social aspects of the sampled species.Laboratório de Cupins Departamento de Biologia Geral e Aplicada Instituto de Biociências Univ Estadual Paulista UNESP Campus Rio Claro, Avenida 24A, 1515, Bela VistaCentro de Estudos de Insetos Sociais-CEIS Instituto de Biociências Univ Estadual Paulista UNESP Campus Rio Claro, Avenida 24A, 1515, Bela VistaLaboratório de Cupins Departamento de Biologia Geral e Aplicada Instituto de Biociências Univ Estadual Paulista UNESP Campus Rio Claro, Avenida 24A, 1515, Bela VistaCentro de Estudos de Insetos Sociais-CEIS Instituto de Biociências Univ Estadual Paulista UNESP Campus Rio Claro, Avenida 24A, 1515, Bela VistaUniversidade Estadual Paulista (Unesp)Costa-Leonardo, Ana Maria [UNESP]da Silva, Iago Bueno [UNESP]Janei, Vanelize [UNESP]Poiani, Silvana Beani [UNESP]dos Santos-Pinto, José Roberto Aparecido [UNESP]Esteves, Franciele Grego [UNESP]Palma, Mario Sérgio [UNESP]2021-06-25T10:59:40Z2021-06-25T10:59:40Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s00441-021-03469-wCell and Tissue Research.1432-08780302-766Xhttp://hdl.handle.net/11449/20771010.1007/s00441-021-03469-w2-s2.0-85105489137Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCell and Tissue Researchinfo:eu-repo/semantics/openAccess2021-10-23T17:45:56Zoai:repositorio.unesp.br:11449/207710Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T21:34:41.011222Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Salivary glands in workers of Ruptitermes spp. (Blattaria, Isoptera, Termitidae, Apicotermitinae): a morphological and preoteomic approach |
title |
Salivary glands in workers of Ruptitermes spp. (Blattaria, Isoptera, Termitidae, Apicotermitinae): a morphological and preoteomic approach |
spellingShingle |
Salivary glands in workers of Ruptitermes spp. (Blattaria, Isoptera, Termitidae, Apicotermitinae): a morphological and preoteomic approach Costa-Leonardo, Ana Maria [UNESP] Central cells LCMS-based proteomics Parietal cells Secretion Toxin-like proteins |
title_short |
Salivary glands in workers of Ruptitermes spp. (Blattaria, Isoptera, Termitidae, Apicotermitinae): a morphological and preoteomic approach |
title_full |
Salivary glands in workers of Ruptitermes spp. (Blattaria, Isoptera, Termitidae, Apicotermitinae): a morphological and preoteomic approach |
title_fullStr |
Salivary glands in workers of Ruptitermes spp. (Blattaria, Isoptera, Termitidae, Apicotermitinae): a morphological and preoteomic approach |
title_full_unstemmed |
Salivary glands in workers of Ruptitermes spp. (Blattaria, Isoptera, Termitidae, Apicotermitinae): a morphological and preoteomic approach |
title_sort |
Salivary glands in workers of Ruptitermes spp. (Blattaria, Isoptera, Termitidae, Apicotermitinae): a morphological and preoteomic approach |
author |
Costa-Leonardo, Ana Maria [UNESP] |
author_facet |
Costa-Leonardo, Ana Maria [UNESP] da Silva, Iago Bueno [UNESP] Janei, Vanelize [UNESP] Poiani, Silvana Beani [UNESP] dos Santos-Pinto, José Roberto Aparecido [UNESP] Esteves, Franciele Grego [UNESP] Palma, Mario Sérgio [UNESP] |
author_role |
author |
author2 |
da Silva, Iago Bueno [UNESP] Janei, Vanelize [UNESP] Poiani, Silvana Beani [UNESP] dos Santos-Pinto, José Roberto Aparecido [UNESP] Esteves, Franciele Grego [UNESP] Palma, Mario Sérgio [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Costa-Leonardo, Ana Maria [UNESP] da Silva, Iago Bueno [UNESP] Janei, Vanelize [UNESP] Poiani, Silvana Beani [UNESP] dos Santos-Pinto, José Roberto Aparecido [UNESP] Esteves, Franciele Grego [UNESP] Palma, Mario Sérgio [UNESP] |
dc.subject.por.fl_str_mv |
Central cells LCMS-based proteomics Parietal cells Secretion Toxin-like proteins |
topic |
Central cells LCMS-based proteomics Parietal cells Secretion Toxin-like proteins |
description |
Salivary glands are omnipresent in termites and occur in all developmental stages and castes. They function to produce, store, and secrete compounds, ranging from a feeding function to defensive mechanisms. Here, we provide a complete morphological overview of the salivary glands in the soldierless species Ruptitermes reconditus and R. xanthochiton, and the first proteomic profile of the salivary glands in a Neotropical Apicotermitinae representative, R. reconditus. Salivary glands from both species were composed of several acini, roughly spherical structures composed of two types of central cells (type I and II) and peripheral parietal cells, as well as transporting ducts and two salivary reservoirs. Central cells were richly supplied with electron-lucent secretory vesicles and rough endoplasmic reticulum, a feature of protein-secreting cells. Parietal cells of Ruptitermes spp. had conspicuous characteristics such as electron-lucent secretory vesicles surrounded by mitochondria and well-developed microvilli. Moreover, different individuals showed variation in the secretory cycle of salivary acini, which may be related to polyethism. Ultrastructural analysis evidenced a high synthesis of secretion and also the occurrence of lysosomes and autophagic structures in central cells. Proteomic analysis of the salivary glands revealed 483 proteins divided into functional groups, highlighting toxins/defensins and compounds related to alarm communication and colony asepsis. Soldierless termites are quite successful, especially due to morphological adaptations of the workers, including unknown modifications of exocrine glands. Thus, according to our morphological and proteomic findings, we discuss the potential roles of the salivary gland secretion in different social aspects of the sampled species. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:59:40Z 2021-06-25T10:59:40Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s00441-021-03469-w Cell and Tissue Research. 1432-0878 0302-766X http://hdl.handle.net/11449/207710 10.1007/s00441-021-03469-w 2-s2.0-85105489137 |
url |
http://dx.doi.org/10.1007/s00441-021-03469-w http://hdl.handle.net/11449/207710 |
identifier_str_mv |
Cell and Tissue Research. 1432-0878 0302-766X 10.1007/s00441-021-03469-w 2-s2.0-85105489137 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cell and Tissue Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129337900138496 |