Proteomic-components provide insights into the defensive secretion in termite workers of the soldierless genus Ruptitermes
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.jprot.2019.103622 http://hdl.handle.net/11449/199861 |
Resumo: | Termite soldiers constitute the defensive frontline of the colonies, despite workers also perform such tasks, especially within the Neotropical Apicotermitinae, in which all species are soldierless. Workers of the genus Ruptitermes display an extreme form of defense, characterized by body rupture and release of a sticky secretion. Previous observations suggested that such behavior may be advantageous against enemies, but the chemical composition of this secretion has been neglected. Here we firstly provide the proteomic profile of the defensive secretion of Ruptitermes reconditus and Ruptitermes pitan workers. Additionally, the mechanisms of action of this behavior was evaluated through different bioassays. A total of 446 proteins were identified in R. reconditus and 391 proteins in R. pitan, which were classified into: toxins, defensins and proteolytic enzymes; sticky components/ alarm communication; proteins related to detoxification processes; proteins involved in folding/conformation and post-translational modifications; housekeeping proteins; and uncharacterized/hypothetical proteins. According to the bioassays, the self-sacrifice is triggered by a physical stimulus, and the defensive secretion may cause immobility and death of the opponents. Assuming that termites are abundant in the tropics and therefore exposed to predators, suicidal behaviors seem to be advantageous, since the loss of an individual benefit the whole colony. Significance: Although recent studies have reported the biochemical composition of different weapons in soldiered species of termites, such efforts had not been applied to sordierless taxa up until now. Thus, this is the first report of the defensive mechanisms in soldierless termite species based on proteomic analysis. The diversity of compounds, which included toxin-like and mucin-like proteins, reflect the mechanisms of action of the defensive secretion released by termite workers, which may cause immobility and death of the opponents. Our findings may contribute to the knowledge regarding the development of defensive strategies in termites, especially in groups which lost the soldier caste during the evolution. |
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Proteomic-components provide insights into the defensive secretion in termite workers of the soldierless genus RuptitermesApicotermitinaeIsopteraLCMS-based proteomicsMucin-like proteinsSticky defensive secretionToxin-like proteinsTermite soldiers constitute the defensive frontline of the colonies, despite workers also perform such tasks, especially within the Neotropical Apicotermitinae, in which all species are soldierless. Workers of the genus Ruptitermes display an extreme form of defense, characterized by body rupture and release of a sticky secretion. Previous observations suggested that such behavior may be advantageous against enemies, but the chemical composition of this secretion has been neglected. Here we firstly provide the proteomic profile of the defensive secretion of Ruptitermes reconditus and Ruptitermes pitan workers. Additionally, the mechanisms of action of this behavior was evaluated through different bioassays. A total of 446 proteins were identified in R. reconditus and 391 proteins in R. pitan, which were classified into: toxins, defensins and proteolytic enzymes; sticky components/ alarm communication; proteins related to detoxification processes; proteins involved in folding/conformation and post-translational modifications; housekeeping proteins; and uncharacterized/hypothetical proteins. According to the bioassays, the self-sacrifice is triggered by a physical stimulus, and the defensive secretion may cause immobility and death of the opponents. Assuming that termites are abundant in the tropics and therefore exposed to predators, suicidal behaviors seem to be advantageous, since the loss of an individual benefit the whole colony. Significance: Although recent studies have reported the biochemical composition of different weapons in soldiered species of termites, such efforts had not been applied to sordierless taxa up until now. Thus, this is the first report of the defensive mechanisms in soldierless termite species based on proteomic analysis. The diversity of compounds, which included toxin-like and mucin-like proteins, reflect the mechanisms of action of the defensive secretion released by termite workers, which may cause immobility and death of the opponents. Our findings may contribute to the knowledge regarding the development of defensive strategies in termites, especially in groups which lost the soldier caste during the evolution.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Laboratório de Cupins Departamento de Biologia Instituto de Biociências Univ Estadual Paulista UNESP, Campus Rio Claro, Avenida 24A, 1515, Bela VistaCenter for the Study of Social Insects Department of Biology Institute of Biosciences of Rio Claro Univ Estadual Paulista UNESP, Rio ClaroLaboratório de Cupins Departamento de Biologia Instituto de Biociências Univ Estadual Paulista UNESP, Campus Rio Claro, Avenida 24A, 1515, Bela VistaCenter for the Study of Social Insects Department of Biology Institute of Biosciences of Rio Claro Univ Estadual Paulista UNESP, Rio ClaroCNPq: 150699/2017-4FAPESP: 2013/26451-9FAPESP: 2016/16212-5FAPESP: 2017/10373-0CAPES: 206/2018CNPq: 301656/2013-4CNPq: 305539/2014-0Universidade Estadual Paulista (Unesp)Costa-Leonardo, Ana Maria [UNESP]da Silva, Iago Bueno [UNESP]Poiani, Silvana Beani [UNESP]dos Santos-Pinto, José Roberto Aparecido [UNESP]Esteves, Franciele Grego [UNESP]da Silva, Luiza Helena Bueno [UNESP]Palma, Mario Sergio [UNESP]2020-12-12T01:51:19Z2020-12-12T01:51:19Z2020-02-20info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.jprot.2019.103622Journal of Proteomics, v. 213.1876-77371874-3919http://hdl.handle.net/11449/19986110.1016/j.jprot.2019.1036222-s2.0-85077060547Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Proteomicsinfo:eu-repo/semantics/openAccess2021-10-23T10:11:13Zoai:repositorio.unesp.br:11449/199861Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T10:11:13Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Proteomic-components provide insights into the defensive secretion in termite workers of the soldierless genus Ruptitermes |
title |
Proteomic-components provide insights into the defensive secretion in termite workers of the soldierless genus Ruptitermes |
spellingShingle |
Proteomic-components provide insights into the defensive secretion in termite workers of the soldierless genus Ruptitermes Costa-Leonardo, Ana Maria [UNESP] Apicotermitinae Isoptera LCMS-based proteomics Mucin-like proteins Sticky defensive secretion Toxin-like proteins |
title_short |
Proteomic-components provide insights into the defensive secretion in termite workers of the soldierless genus Ruptitermes |
title_full |
Proteomic-components provide insights into the defensive secretion in termite workers of the soldierless genus Ruptitermes |
title_fullStr |
Proteomic-components provide insights into the defensive secretion in termite workers of the soldierless genus Ruptitermes |
title_full_unstemmed |
Proteomic-components provide insights into the defensive secretion in termite workers of the soldierless genus Ruptitermes |
title_sort |
Proteomic-components provide insights into the defensive secretion in termite workers of the soldierless genus Ruptitermes |
author |
Costa-Leonardo, Ana Maria [UNESP] |
author_facet |
Costa-Leonardo, Ana Maria [UNESP] da Silva, Iago Bueno [UNESP] Poiani, Silvana Beani [UNESP] dos Santos-Pinto, José Roberto Aparecido [UNESP] Esteves, Franciele Grego [UNESP] da Silva, Luiza Helena Bueno [UNESP] Palma, Mario Sergio [UNESP] |
author_role |
author |
author2 |
da Silva, Iago Bueno [UNESP] Poiani, Silvana Beani [UNESP] dos Santos-Pinto, José Roberto Aparecido [UNESP] Esteves, Franciele Grego [UNESP] da Silva, Luiza Helena Bueno [UNESP] Palma, Mario Sergio [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Costa-Leonardo, Ana Maria [UNESP] da Silva, Iago Bueno [UNESP] Poiani, Silvana Beani [UNESP] dos Santos-Pinto, José Roberto Aparecido [UNESP] Esteves, Franciele Grego [UNESP] da Silva, Luiza Helena Bueno [UNESP] Palma, Mario Sergio [UNESP] |
dc.subject.por.fl_str_mv |
Apicotermitinae Isoptera LCMS-based proteomics Mucin-like proteins Sticky defensive secretion Toxin-like proteins |
topic |
Apicotermitinae Isoptera LCMS-based proteomics Mucin-like proteins Sticky defensive secretion Toxin-like proteins |
description |
Termite soldiers constitute the defensive frontline of the colonies, despite workers also perform such tasks, especially within the Neotropical Apicotermitinae, in which all species are soldierless. Workers of the genus Ruptitermes display an extreme form of defense, characterized by body rupture and release of a sticky secretion. Previous observations suggested that such behavior may be advantageous against enemies, but the chemical composition of this secretion has been neglected. Here we firstly provide the proteomic profile of the defensive secretion of Ruptitermes reconditus and Ruptitermes pitan workers. Additionally, the mechanisms of action of this behavior was evaluated through different bioassays. A total of 446 proteins were identified in R. reconditus and 391 proteins in R. pitan, which were classified into: toxins, defensins and proteolytic enzymes; sticky components/ alarm communication; proteins related to detoxification processes; proteins involved in folding/conformation and post-translational modifications; housekeeping proteins; and uncharacterized/hypothetical proteins. According to the bioassays, the self-sacrifice is triggered by a physical stimulus, and the defensive secretion may cause immobility and death of the opponents. Assuming that termites are abundant in the tropics and therefore exposed to predators, suicidal behaviors seem to be advantageous, since the loss of an individual benefit the whole colony. Significance: Although recent studies have reported the biochemical composition of different weapons in soldiered species of termites, such efforts had not been applied to sordierless taxa up until now. Thus, this is the first report of the defensive mechanisms in soldierless termite species based on proteomic analysis. The diversity of compounds, which included toxin-like and mucin-like proteins, reflect the mechanisms of action of the defensive secretion released by termite workers, which may cause immobility and death of the opponents. Our findings may contribute to the knowledge regarding the development of defensive strategies in termites, especially in groups which lost the soldier caste during the evolution. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-12T01:51:19Z 2020-12-12T01:51:19Z 2020-02-20 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.jprot.2019.103622 Journal of Proteomics, v. 213. 1876-7737 1874-3919 http://hdl.handle.net/11449/199861 10.1016/j.jprot.2019.103622 2-s2.0-85077060547 |
url |
http://dx.doi.org/10.1016/j.jprot.2019.103622 http://hdl.handle.net/11449/199861 |
identifier_str_mv |
Journal of Proteomics, v. 213. 1876-7737 1874-3919 10.1016/j.jprot.2019.103622 2-s2.0-85077060547 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Proteomics |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965205415854080 |