Serum protein concentration in newborn calves fed with colostrum from cows with mastitis
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.11606/issn.1678-4456.v50i3p188-197 http://hdl.handle.net/11449/227318 |
Resumo: | The aim of this study was to evaluate the serum protein concentration in newborns fed with colostrum derived from healthy cows (n = 10), cows with subclinical mastitis (n = 10) and cows with clinical mastitis (n = 10). 30 Holstein cows were assigned to their respective groups according to macroscopic examination of colostral secretion, somatic cell count, CMT and presence of bacteria in colostrum samples. Blood samples of the calves were collected immediately after birth, at 24 and 48 hours after ingestion of colostrum. The total protein was measured by the biuret method and the concentrations of immunoglobulin A (IgA), immunoglobulin G (IgG), transferrin, albumin and haptoglobin was determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). No differences were observed amongst groups in the concentrations of albumin, total protein and IgA. In animals from cows with subclinical and clinical mastitis haptoglobin concentrations were higher than those of healthy animals. The concentrations of IgG and transferrin were significantly lower in calves from cows with mastitis. We concluded that the ingestion of colostrum from infected and uninfected glands from cows with mastitis (GII e GIII) is unlikely to be an important contributor to the high rate of failure of passive transfer of immunoglobulins in calves. |
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Serum protein concentration in newborn calves fed with colostrum from cows with mastitisProteinograma sérico de bezerros recém-nascidos alimentados com colostro de vacas com mastiteColostrumImmunoglobulinsMastitisPassive transfer immunityThe aim of this study was to evaluate the serum protein concentration in newborns fed with colostrum derived from healthy cows (n = 10), cows with subclinical mastitis (n = 10) and cows with clinical mastitis (n = 10). 30 Holstein cows were assigned to their respective groups according to macroscopic examination of colostral secretion, somatic cell count, CMT and presence of bacteria in colostrum samples. Blood samples of the calves were collected immediately after birth, at 24 and 48 hours after ingestion of colostrum. The total protein was measured by the biuret method and the concentrations of immunoglobulin A (IgA), immunoglobulin G (IgG), transferrin, albumin and haptoglobin was determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). No differences were observed amongst groups in the concentrations of albumin, total protein and IgA. In animals from cows with subclinical and clinical mastitis haptoglobin concentrations were higher than those of healthy animals. The concentrations of IgG and transferrin were significantly lower in calves from cows with mastitis. We concluded that the ingestion of colostrum from infected and uninfected glands from cows with mastitis (GII e GIII) is unlikely to be an important contributor to the high rate of failure of passive transfer of immunoglobulins in calves.UNESP, Araçatuba - SPDepartamento de Apoio da Produção e Saúde Animal UNESP, Araçatuba - SPDepartamento de Clínica Cirurgia e Reprodução Animal UNESP, Araçatuba - SPUNESP, Araçatuba - SPDepartamento de Apoio da Produção e Saúde Animal UNESP, Araçatuba - SPDepartamento de Clínica Cirurgia e Reprodução Animal UNESP, Araçatuba - SPUniversidade Estadual Paulista (UNESP)Santos, Guilherme Gonçalves Fabretti [UNESP]Deschk, Maurício [UNESP]Silva, André Kielius Guedes [UNESP]Pólo, Tatiane Silva [UNESP]Marinho, Márcia [UNESP]Peiró, Juliana Regina [UNESP]Mendes, Luiz Cláudio Nogueira [UNESP]Feitosa, Francisco Leydson Formiga [UNESP]2022-04-29T07:12:41Z2022-04-29T07:12:41Z2013-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article188-197http://dx.doi.org/10.11606/issn.1678-4456.v50i3p188-197Brazilian Journal of Veterinary Research and Animal Science, v. 50, n. 3, p. 188-197, 2013.1678-44561413-9596http://hdl.handle.net/11449/22731810.11606/issn.1678-4456.v50i3p188-1972-s2.0-84888118888Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporBrazilian Journal of Veterinary Research and Animal Scienceinfo:eu-repo/semantics/openAccess2022-04-29T07:12:42Zoai:repositorio.unesp.br:11449/227318Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-29T07:12:42Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Serum protein concentration in newborn calves fed with colostrum from cows with mastitis Proteinograma sérico de bezerros recém-nascidos alimentados com colostro de vacas com mastite |
title |
Serum protein concentration in newborn calves fed with colostrum from cows with mastitis |
spellingShingle |
Serum protein concentration in newborn calves fed with colostrum from cows with mastitis Santos, Guilherme Gonçalves Fabretti [UNESP] Colostrum Immunoglobulins Mastitis Passive transfer immunity |
title_short |
Serum protein concentration in newborn calves fed with colostrum from cows with mastitis |
title_full |
Serum protein concentration in newborn calves fed with colostrum from cows with mastitis |
title_fullStr |
Serum protein concentration in newborn calves fed with colostrum from cows with mastitis |
title_full_unstemmed |
Serum protein concentration in newborn calves fed with colostrum from cows with mastitis |
title_sort |
Serum protein concentration in newborn calves fed with colostrum from cows with mastitis |
author |
Santos, Guilherme Gonçalves Fabretti [UNESP] |
author_facet |
Santos, Guilherme Gonçalves Fabretti [UNESP] Deschk, Maurício [UNESP] Silva, André Kielius Guedes [UNESP] Pólo, Tatiane Silva [UNESP] Marinho, Márcia [UNESP] Peiró, Juliana Regina [UNESP] Mendes, Luiz Cláudio Nogueira [UNESP] Feitosa, Francisco Leydson Formiga [UNESP] |
author_role |
author |
author2 |
Deschk, Maurício [UNESP] Silva, André Kielius Guedes [UNESP] Pólo, Tatiane Silva [UNESP] Marinho, Márcia [UNESP] Peiró, Juliana Regina [UNESP] Mendes, Luiz Cláudio Nogueira [UNESP] Feitosa, Francisco Leydson Formiga [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Santos, Guilherme Gonçalves Fabretti [UNESP] Deschk, Maurício [UNESP] Silva, André Kielius Guedes [UNESP] Pólo, Tatiane Silva [UNESP] Marinho, Márcia [UNESP] Peiró, Juliana Regina [UNESP] Mendes, Luiz Cláudio Nogueira [UNESP] Feitosa, Francisco Leydson Formiga [UNESP] |
dc.subject.por.fl_str_mv |
Colostrum Immunoglobulins Mastitis Passive transfer immunity |
topic |
Colostrum Immunoglobulins Mastitis Passive transfer immunity |
description |
The aim of this study was to evaluate the serum protein concentration in newborns fed with colostrum derived from healthy cows (n = 10), cows with subclinical mastitis (n = 10) and cows with clinical mastitis (n = 10). 30 Holstein cows were assigned to their respective groups according to macroscopic examination of colostral secretion, somatic cell count, CMT and presence of bacteria in colostrum samples. Blood samples of the calves were collected immediately after birth, at 24 and 48 hours after ingestion of colostrum. The total protein was measured by the biuret method and the concentrations of immunoglobulin A (IgA), immunoglobulin G (IgG), transferrin, albumin and haptoglobin was determined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). No differences were observed amongst groups in the concentrations of albumin, total protein and IgA. In animals from cows with subclinical and clinical mastitis haptoglobin concentrations were higher than those of healthy animals. The concentrations of IgG and transferrin were significantly lower in calves from cows with mastitis. We concluded that the ingestion of colostrum from infected and uninfected glands from cows with mastitis (GII e GIII) is unlikely to be an important contributor to the high rate of failure of passive transfer of immunoglobulins in calves. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-01-01 2022-04-29T07:12:41Z 2022-04-29T07:12:41Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.11606/issn.1678-4456.v50i3p188-197 Brazilian Journal of Veterinary Research and Animal Science, v. 50, n. 3, p. 188-197, 2013. 1678-4456 1413-9596 http://hdl.handle.net/11449/227318 10.11606/issn.1678-4456.v50i3p188-197 2-s2.0-84888118888 |
url |
http://dx.doi.org/10.11606/issn.1678-4456.v50i3p188-197 http://hdl.handle.net/11449/227318 |
identifier_str_mv |
Brazilian Journal of Veterinary Research and Animal Science, v. 50, n. 3, p. 188-197, 2013. 1678-4456 1413-9596 10.11606/issn.1678-4456.v50i3p188-197 2-s2.0-84888118888 |
dc.language.iso.fl_str_mv |
eng por |
language |
eng por |
dc.relation.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
188-197 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1797790094420082688 |