Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss)
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1007/s11356-021-14583-1 http://hdl.handle.net/11449/207771 |
Resumo: | Fish have defense systems that are capable of repairing damages caused by xenobiotics like benzo[a]pyrene (BaP), so the aims of this study were to identify BaP toxicity in melanomacrophages (MMs) cytoskeleton, evaluate the melanin area in MMs, and analyze genotoxicity. Rainbow trout juveniles (n = 24) were split in 48h and 7d treatments that received 2 mg/kg of BaP. After the experiment, blood samples were collected and liver was removed, to proceed with the analysis: EROD activity, MMs melanin area quantification, melanosomes movements, and a genotoxicity test. The results revealed increased in EROD activity after 48-h and 7-day BaP exposure. The group 7d displayed a reduction in MMs pigmented area, melanosomes aggregation, in addition to an increased frequency of micronucleus. By means of the EROD assay, it was possible to confirm the activation of BaP biotransformation system. The impairment of the melanosomes’ movements possibly by an inactivation of the protein responsible for the pigment dispersion consequently affects the melanin area and thus might negatively impact the MMs detoxification capacity. In addition to this cytotoxicity, the increased frequency of micronucleus might also indicate the genotoxicity of BaP in this important fish species. |
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Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss)CytoskeletonERODErythrocytes abnormalitiesLiverMelanomacrophageMorphological alterationFish have defense systems that are capable of repairing damages caused by xenobiotics like benzo[a]pyrene (BaP), so the aims of this study were to identify BaP toxicity in melanomacrophages (MMs) cytoskeleton, evaluate the melanin area in MMs, and analyze genotoxicity. Rainbow trout juveniles (n = 24) were split in 48h and 7d treatments that received 2 mg/kg of BaP. After the experiment, blood samples were collected and liver was removed, to proceed with the analysis: EROD activity, MMs melanin area quantification, melanosomes movements, and a genotoxicity test. The results revealed increased in EROD activity after 48-h and 7-day BaP exposure. The group 7d displayed a reduction in MMs pigmented area, melanosomes aggregation, in addition to an increased frequency of micronucleus. By means of the EROD assay, it was possible to confirm the activation of BaP biotransformation system. The impairment of the melanosomes’ movements possibly by an inactivation of the protein responsible for the pigment dispersion consequently affects the melanin area and thus might negatively impact the MMs detoxification capacity. In addition to this cytotoxicity, the increased frequency of micronucleus might also indicate the genotoxicity of BaP in this important fish species.Graduate Program in Animal Biology Universidade Estadual Paulista (UNESP), São José do Rio PretoDepartment of Biology Universidade Estadual Paulista (UNESP), São José do Rio PretoDepartment of Biological and Environmental Sciences University of Gothenburg, Box 463Graduate Program in Animal Biology Universidade Estadual Paulista (UNESP), São José do Rio PretoDepartment of Biology Universidade Estadual Paulista (UNESP), São José do Rio PretoUniversidade Estadual Paulista (Unesp)University of GothenburgFanali, Lara Zácari [UNESP]De Oliveira, Classius [UNESP]Sturve, Joachim2021-06-25T11:00:45Z2021-06-25T11:00:45Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1007/s11356-021-14583-1Environmental Science and Pollution Research.1614-74990944-1344http://hdl.handle.net/11449/20777110.1007/s11356-021-14583-12-s2.0-8510645489457318566502178590000-0002-2029-7900Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEnvironmental Science and Pollution Researchinfo:eu-repo/semantics/openAccess2021-10-23T17:45:59Zoai:repositorio.unesp.br:11449/207771Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:09:02.597519Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss) |
title |
Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss) |
spellingShingle |
Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss) Fanali, Lara Zácari [UNESP] Cytoskeleton EROD Erythrocytes abnormalities Liver Melanomacrophage Morphological alteration |
title_short |
Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss) |
title_full |
Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss) |
title_fullStr |
Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss) |
title_full_unstemmed |
Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss) |
title_sort |
Enzymatic, morphological, and genotoxic effects of benzo[a]pyrene in rainbow trout (Oncorhynchus mykiss) |
author |
Fanali, Lara Zácari [UNESP] |
author_facet |
Fanali, Lara Zácari [UNESP] De Oliveira, Classius [UNESP] Sturve, Joachim |
author_role |
author |
author2 |
De Oliveira, Classius [UNESP] Sturve, Joachim |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) University of Gothenburg |
dc.contributor.author.fl_str_mv |
Fanali, Lara Zácari [UNESP] De Oliveira, Classius [UNESP] Sturve, Joachim |
dc.subject.por.fl_str_mv |
Cytoskeleton EROD Erythrocytes abnormalities Liver Melanomacrophage Morphological alteration |
topic |
Cytoskeleton EROD Erythrocytes abnormalities Liver Melanomacrophage Morphological alteration |
description |
Fish have defense systems that are capable of repairing damages caused by xenobiotics like benzo[a]pyrene (BaP), so the aims of this study were to identify BaP toxicity in melanomacrophages (MMs) cytoskeleton, evaluate the melanin area in MMs, and analyze genotoxicity. Rainbow trout juveniles (n = 24) were split in 48h and 7d treatments that received 2 mg/kg of BaP. After the experiment, blood samples were collected and liver was removed, to proceed with the analysis: EROD activity, MMs melanin area quantification, melanosomes movements, and a genotoxicity test. The results revealed increased in EROD activity after 48-h and 7-day BaP exposure. The group 7d displayed a reduction in MMs pigmented area, melanosomes aggregation, in addition to an increased frequency of micronucleus. By means of the EROD assay, it was possible to confirm the activation of BaP biotransformation system. The impairment of the melanosomes’ movements possibly by an inactivation of the protein responsible for the pigment dispersion consequently affects the melanin area and thus might negatively impact the MMs detoxification capacity. In addition to this cytotoxicity, the increased frequency of micronucleus might also indicate the genotoxicity of BaP in this important fish species. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T11:00:45Z 2021-06-25T11:00:45Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1007/s11356-021-14583-1 Environmental Science and Pollution Research. 1614-7499 0944-1344 http://hdl.handle.net/11449/207771 10.1007/s11356-021-14583-1 2-s2.0-85106454894 5731856650217859 0000-0002-2029-7900 |
url |
http://dx.doi.org/10.1007/s11356-021-14583-1 http://hdl.handle.net/11449/207771 |
identifier_str_mv |
Environmental Science and Pollution Research. 1614-7499 0944-1344 10.1007/s11356-021-14583-1 2-s2.0-85106454894 5731856650217859 0000-0002-2029-7900 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Environmental Science and Pollution Research |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128467294748672 |