Decrease in streptococcus mutans after applying sealants to the occlusal surfaces of permanent teeth in adults
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/232953 |
Resumo: | Introduction: The main strategy for the prevention of caries disease is the use of pit and fissure sealants, which are indicated for posterior occlusal surfaces that are difficult to clean and cannot be protected very well by fluoride. This strategy is considered to be very important in caries prevention, especially in patients at high cariogenic risk. Objective: Evaluate whether the count of colony forming units of S. mutans per cm2 on the occlusal surface of posterior permanent teeth changes after applying resin-based sealant. Methods: A study was conducted of 38 patients of both genders aged 18-30 years. The patients were at high cariogenic risk and had an indication of occlusal sealant application on at least one permanent posterior tooth. Two samples were taken of dental biofilm from the same teeth before (group T0) and after (group T1) applying sealant obtained by direct impression by tray technique with trypticase yeast extract, cysteine, sucrose with bacitracin (TYCSB), and agar previously made and solidified. The samples were incubated at 37 ºC for 48 hours. The colony forming units (CFU/cm2) were counted. The results were statistically analyzed with the Wilcoxon test at 95 % confidence. Results: Average expression in CFU/cm2 before applying the sealant (T0) and standard deviation was 13.48 (± 14.2), whereas after applying the sealant (T1) it was 5.37 (± 8.90). There was a statistical difference between the two measures T0 and T1 (p< 0.05). Conclusions: Sealant application on occlusal surfaces of posterior permanent teeth significantly reduces the count of CFU/cm2 of S. mutans. |
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Decrease in streptococcus mutans after applying sealants to the occlusal surfaces of permanent teeth in adultsDecrecimiento de streptococcus mutans después de la aplicación de sellantes en superficies oclusales de molares permanentes en adultosComposite resinsPermanent dental restorationPit and fissure sealantsStreptococcus mutansIntroduction: The main strategy for the prevention of caries disease is the use of pit and fissure sealants, which are indicated for posterior occlusal surfaces that are difficult to clean and cannot be protected very well by fluoride. This strategy is considered to be very important in caries prevention, especially in patients at high cariogenic risk. Objective: Evaluate whether the count of colony forming units of S. mutans per cm2 on the occlusal surface of posterior permanent teeth changes after applying resin-based sealant. Methods: A study was conducted of 38 patients of both genders aged 18-30 years. The patients were at high cariogenic risk and had an indication of occlusal sealant application on at least one permanent posterior tooth. Two samples were taken of dental biofilm from the same teeth before (group T0) and after (group T1) applying sealant obtained by direct impression by tray technique with trypticase yeast extract, cysteine, sucrose with bacitracin (TYCSB), and agar previously made and solidified. The samples were incubated at 37 ºC for 48 hours. The colony forming units (CFU/cm2) were counted. The results were statistically analyzed with the Wilcoxon test at 95 % confidence. Results: Average expression in CFU/cm2 before applying the sealant (T0) and standard deviation was 13.48 (± 14.2), whereas after applying the sealant (T1) it was 5.37 (± 8.90). There was a statistical difference between the two measures T0 and T1 (p< 0.05). Conclusions: Sealant application on occlusal surfaces of posterior permanent teeth significantly reduces the count of CFU/cm2 of S. mutans.Universidad de Chile Facultad de Odontología Departamento de Odontología RestauradoraUniversidad Andres Bello Facultad de OdontologíaUniversidad Autónoma de Chile Instituto de Ciencias BiomédicasUniversidad Estadual Paulista Facultad de Odontología Departamento de Odontología RestauradoraUniversidad Politécnica y Artística del ParaguayUniversidad de Chile. Facultad de Microbiología Dental Departamento de MEdicina y Patología OralUniversidad de Ciencias Médicas de La Habana Facultad de Ciencias Médicas “Victoria de Girón” Departamento de Estomatología General IntegralUniversidad Estadual Paulista Facultad de Odontología Departamento de Odontología RestauradoraFacultad de OdontologíaInstituto de Ciencias BiomédicasUniversidade Estadual Paulista (UNESP)Universidad Politécnica y Artística del ParaguayUniversidad de Chile. Facultad de Microbiología DentalFacultad de Ciencias Médicas “Victoria de Girón”Grez, Patricio VildósolaGodoy, Eduardo FernandezSaad, Jose Roberto Cury [UNESP]Fluxá, Patricia PalmaGil, Alain Manuel ChapleZepeda, Natalia AcuñaCortés, Gustavo Moncada2022-04-30T22:28:32Z2022-04-30T22:28:32Z2019-10-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Cubana de Estomatologia, v. 56, n. 4, 2019.1561-297X0034-7507http://hdl.handle.net/11449/2329532-s2.0-85077581199Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengRevista Cubana de Estomatologiainfo:eu-repo/semantics/openAccess2022-04-30T22:28:32Zoai:repositorio.unesp.br:11449/232953Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T15:45:34.669779Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Decrease in streptococcus mutans after applying sealants to the occlusal surfaces of permanent teeth in adults Decrecimiento de streptococcus mutans después de la aplicación de sellantes en superficies oclusales de molares permanentes en adultos |
title |
Decrease in streptococcus mutans after applying sealants to the occlusal surfaces of permanent teeth in adults |
spellingShingle |
Decrease in streptococcus mutans after applying sealants to the occlusal surfaces of permanent teeth in adults Grez, Patricio Vildósola Composite resins Permanent dental restoration Pit and fissure sealants Streptococcus mutans |
title_short |
Decrease in streptococcus mutans after applying sealants to the occlusal surfaces of permanent teeth in adults |
title_full |
Decrease in streptococcus mutans after applying sealants to the occlusal surfaces of permanent teeth in adults |
title_fullStr |
Decrease in streptococcus mutans after applying sealants to the occlusal surfaces of permanent teeth in adults |
title_full_unstemmed |
Decrease in streptococcus mutans after applying sealants to the occlusal surfaces of permanent teeth in adults |
title_sort |
Decrease in streptococcus mutans after applying sealants to the occlusal surfaces of permanent teeth in adults |
author |
Grez, Patricio Vildósola |
author_facet |
Grez, Patricio Vildósola Godoy, Eduardo Fernandez Saad, Jose Roberto Cury [UNESP] Fluxá, Patricia Palma Gil, Alain Manuel Chaple Zepeda, Natalia Acuña Cortés, Gustavo Moncada |
author_role |
author |
author2 |
Godoy, Eduardo Fernandez Saad, Jose Roberto Cury [UNESP] Fluxá, Patricia Palma Gil, Alain Manuel Chaple Zepeda, Natalia Acuña Cortés, Gustavo Moncada |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Facultad de Odontología Instituto de Ciencias Biomédicas Universidade Estadual Paulista (UNESP) Universidad Politécnica y Artística del Paraguay Universidad de Chile. Facultad de Microbiología Dental Facultad de Ciencias Médicas “Victoria de Girón” |
dc.contributor.author.fl_str_mv |
Grez, Patricio Vildósola Godoy, Eduardo Fernandez Saad, Jose Roberto Cury [UNESP] Fluxá, Patricia Palma Gil, Alain Manuel Chaple Zepeda, Natalia Acuña Cortés, Gustavo Moncada |
dc.subject.por.fl_str_mv |
Composite resins Permanent dental restoration Pit and fissure sealants Streptococcus mutans |
topic |
Composite resins Permanent dental restoration Pit and fissure sealants Streptococcus mutans |
description |
Introduction: The main strategy for the prevention of caries disease is the use of pit and fissure sealants, which are indicated for posterior occlusal surfaces that are difficult to clean and cannot be protected very well by fluoride. This strategy is considered to be very important in caries prevention, especially in patients at high cariogenic risk. Objective: Evaluate whether the count of colony forming units of S. mutans per cm2 on the occlusal surface of posterior permanent teeth changes after applying resin-based sealant. Methods: A study was conducted of 38 patients of both genders aged 18-30 years. The patients were at high cariogenic risk and had an indication of occlusal sealant application on at least one permanent posterior tooth. Two samples were taken of dental biofilm from the same teeth before (group T0) and after (group T1) applying sealant obtained by direct impression by tray technique with trypticase yeast extract, cysteine, sucrose with bacitracin (TYCSB), and agar previously made and solidified. The samples were incubated at 37 ºC for 48 hours. The colony forming units (CFU/cm2) were counted. The results were statistically analyzed with the Wilcoxon test at 95 % confidence. Results: Average expression in CFU/cm2 before applying the sealant (T0) and standard deviation was 13.48 (± 14.2), whereas after applying the sealant (T1) it was 5.37 (± 8.90). There was a statistical difference between the two measures T0 and T1 (p< 0.05). Conclusions: Sealant application on occlusal surfaces of posterior permanent teeth significantly reduces the count of CFU/cm2 of S. mutans. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-01 2022-04-30T22:28:32Z 2022-04-30T22:28:32Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Revista Cubana de Estomatologia, v. 56, n. 4, 2019. 1561-297X 0034-7507 http://hdl.handle.net/11449/232953 2-s2.0-85077581199 |
identifier_str_mv |
Revista Cubana de Estomatologia, v. 56, n. 4, 2019. 1561-297X 0034-7507 2-s2.0-85077581199 |
url |
http://hdl.handle.net/11449/232953 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Revista Cubana de Estomatologia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128557364281344 |