Characterization of Ralstonia solanacearum strains from Brazil using molecular methods and pathogenicity tests
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/227106 |
Resumo: | Forty one Brazilian strains of Ralstonia solanacearum from ornamental plants (Begonia sp., Fuchsia sp., Oxalis sp., Pelargonium x hortorum and Tagetes sp.), eucalyptus (Eucalyptus sp.), Solanaceae (Nicotiana tabacum, Solanum jilo, S. lycopersicum, S. melongena, S. tuberosum), banana (Musa sp.) and heliconia (Heliconia sp.), belonging to three different races and five biovars, were characterized by ERIC and BOX-PCR and classified in phylotypes by sequence analysis of the endoglucanase (Egl) and MutS genes. The virulence of 22 strains was evaluated on tomato cv. Petomech VF, potato cv. Agata and tobacco var. Burley. In the ERIC and BOX-PCR analysis, strains were separated into two main clusters showing high genetic diversity among them, with no correlation concerning race, biovar or geographical origin. Phylogenetic analysis based on sequences of part of the Egl and MutS genes revealed the predominance of phylotype II in Brazil. With exception of strains belonging to race 2, which were not included in the experiments, pathogenicity tests carried out with different strains of R. solanacearum showed high virulence on tomato and potato, and lower virulence on tobacco, showing no relationship with race, biovar or host plant from which the tested strain were originally isolated. |
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Characterization of Ralstonia solanacearum strains from Brazil using molecular methods and pathogenicity testsBacterial wiltGenetic diversityPhylotypesVirulenceForty one Brazilian strains of Ralstonia solanacearum from ornamental plants (Begonia sp., Fuchsia sp., Oxalis sp., Pelargonium x hortorum and Tagetes sp.), eucalyptus (Eucalyptus sp.), Solanaceae (Nicotiana tabacum, Solanum jilo, S. lycopersicum, S. melongena, S. tuberosum), banana (Musa sp.) and heliconia (Heliconia sp.), belonging to three different races and five biovars, were characterized by ERIC and BOX-PCR and classified in phylotypes by sequence analysis of the endoglucanase (Egl) and MutS genes. The virulence of 22 strains was evaluated on tomato cv. Petomech VF, potato cv. Agata and tobacco var. Burley. In the ERIC and BOX-PCR analysis, strains were separated into two main clusters showing high genetic diversity among them, with no correlation concerning race, biovar or geographical origin. Phylogenetic analysis based on sequences of part of the Egl and MutS genes revealed the predominance of phylotype II in Brazil. With exception of strains belonging to race 2, which were not included in the experiments, pathogenicity tests carried out with different strains of R. solanacearum showed high virulence on tomato and potato, and lower virulence on tobacco, showing no relationship with race, biovar or host plant from which the tested strain were originally isolated.Departament of Plant Protection College of Agriculture Sciences-UNESP, C.P. 237, 18.603-970, Botucatu-SPLaboratory of Plant Bacteriology Biological Institute, Campinas-SPCenter of Molecular Biology and Genetic Engineering UNICAMP, Campinas-SPDepartament of Plant Protection College of Agriculture Sciences-UNESP, C.P. 237, 18.603-970, Botucatu-SPUniversidade Estadual Paulista (UNESP)Biological InstituteUniversidade Estadual de Campinas (UNICAMP)Rodrigues, L. M.R. [UNESP]Destéfano, S. A.L.da Silva, M. J.Costa, G. G.L.Maringoni, A. C. [UNESP]2022-04-29T06:48:17Z2022-04-29T06:48:17Z2012-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article505-516Journal of Plant Pathology, v. 94, n. 3, p. 505-516, 2012.1125-4653http://hdl.handle.net/11449/2271062-s2.0-84872388384Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Plant Pathologyinfo:eu-repo/semantics/openAccess2024-04-30T18:07:32Zoai:repositorio.unesp.br:11449/227106Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-04-30T18:07:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Characterization of Ralstonia solanacearum strains from Brazil using molecular methods and pathogenicity tests |
title |
Characterization of Ralstonia solanacearum strains from Brazil using molecular methods and pathogenicity tests |
spellingShingle |
Characterization of Ralstonia solanacearum strains from Brazil using molecular methods and pathogenicity tests Rodrigues, L. M.R. [UNESP] Bacterial wilt Genetic diversity Phylotypes Virulence |
title_short |
Characterization of Ralstonia solanacearum strains from Brazil using molecular methods and pathogenicity tests |
title_full |
Characterization of Ralstonia solanacearum strains from Brazil using molecular methods and pathogenicity tests |
title_fullStr |
Characterization of Ralstonia solanacearum strains from Brazil using molecular methods and pathogenicity tests |
title_full_unstemmed |
Characterization of Ralstonia solanacearum strains from Brazil using molecular methods and pathogenicity tests |
title_sort |
Characterization of Ralstonia solanacearum strains from Brazil using molecular methods and pathogenicity tests |
author |
Rodrigues, L. M.R. [UNESP] |
author_facet |
Rodrigues, L. M.R. [UNESP] Destéfano, S. A.L. da Silva, M. J. Costa, G. G.L. Maringoni, A. C. [UNESP] |
author_role |
author |
author2 |
Destéfano, S. A.L. da Silva, M. J. Costa, G. G.L. Maringoni, A. C. [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Biological Institute Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Rodrigues, L. M.R. [UNESP] Destéfano, S. A.L. da Silva, M. J. Costa, G. G.L. Maringoni, A. C. [UNESP] |
dc.subject.por.fl_str_mv |
Bacterial wilt Genetic diversity Phylotypes Virulence |
topic |
Bacterial wilt Genetic diversity Phylotypes Virulence |
description |
Forty one Brazilian strains of Ralstonia solanacearum from ornamental plants (Begonia sp., Fuchsia sp., Oxalis sp., Pelargonium x hortorum and Tagetes sp.), eucalyptus (Eucalyptus sp.), Solanaceae (Nicotiana tabacum, Solanum jilo, S. lycopersicum, S. melongena, S. tuberosum), banana (Musa sp.) and heliconia (Heliconia sp.), belonging to three different races and five biovars, were characterized by ERIC and BOX-PCR and classified in phylotypes by sequence analysis of the endoglucanase (Egl) and MutS genes. The virulence of 22 strains was evaluated on tomato cv. Petomech VF, potato cv. Agata and tobacco var. Burley. In the ERIC and BOX-PCR analysis, strains were separated into two main clusters showing high genetic diversity among them, with no correlation concerning race, biovar or geographical origin. Phylogenetic analysis based on sequences of part of the Egl and MutS genes revealed the predominance of phylotype II in Brazil. With exception of strains belonging to race 2, which were not included in the experiments, pathogenicity tests carried out with different strains of R. solanacearum showed high virulence on tomato and potato, and lower virulence on tobacco, showing no relationship with race, biovar or host plant from which the tested strain were originally isolated. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-01 2022-04-29T06:48:17Z 2022-04-29T06:48:17Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Journal of Plant Pathology, v. 94, n. 3, p. 505-516, 2012. 1125-4653 http://hdl.handle.net/11449/227106 2-s2.0-84872388384 |
identifier_str_mv |
Journal of Plant Pathology, v. 94, n. 3, p. 505-516, 2012. 1125-4653 2-s2.0-84872388384 |
url |
http://hdl.handle.net/11449/227106 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Plant Pathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
505-516 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965247090458624 |