Annexin 1: differential expression in tumor and mast cells in human larynx cancer

• Autor(a) principal: Silistino-Souza, Rosana
• Data de Publicação: 2007
• Outros Autores: Rodrigues-Lisoni, Flavia C., Cury, Patricia M., Maniglia, Jose V., Raposo, Luis S., Tajara, Eloiza H., Christian, Helen C., Oliani, Sonia M.
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1002/ijc.22639; http://hdl.handle.net/11449/21381
• topic: human mast cells; Hep-2 cells; annexin 1; larynx tumor; ultrastructural immunocytochemistry

Annexin 1 protein (ANXA1) expression was evaluated in tumor and mast cells in human larynx cancer and control epithelium. The effect of the exogenous ANXA1 (peptide Ac 2-26) was also examined during the cellular growth of the Hep-2 human larynx epidermoid carcinoma cell line. This peptide inhibited the proliferation of the Hep-2 cells within 144 hr. In surgical tissue specimens from 20 patients with larynx cancer, ultrastructural immunocytochemistry analysis showed in vivo down-regulation of ANXA1 expression in the tumor and increased in mast cells and Hep-2 cells treated with peptide Ac2-26. Combined in vivo and in vitro analysis demonstrated that ANXA1 plays a regulatory role in laryngeal cancer cell growth. We believe that a better understanding of the regulatory mechanisms of ANXA1 in tumor and mast cells may lead to future biological targets for the therapeutic intervention of human larynx cancer. (c) 2007 Wiley-Liss, Inc.