Hyperglycemic condition during puberty increases collagen fibers deposition in the prostatic stroma and reduces MMP-2 activity
Autor(a) principal: | |
---|---|
Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bbrc.2017.10.022 http://hdl.handle.net/11449/179264 |
Resumo: | Puberty is an important period for the growth and maturation of the male reproductive system, and is also a critical window for endocrine or environmental interference. The physiological levels of circulating insulin and hyperglycemic control are important factors for a normal prostate growth. Hyperglycemia during puberty is reported to retard the growth of the prostate gland, with remarkable effects on the epithelial compartment. Here, we investigated the impact of hyperglycemia along with a simultaneous or late insulin replacement on the ventral prostate growth in rats during puberty, paying special attention to the deposition of collagen fibers and activities of gelatinase, matrix metalloproteinase-2 (MMP-2), and −9 (MMP-9). Hyperglycemia was induced by streptozotocin (STZ) administration in 40-day-old male Wistar rats. A subset of hyperglycemic rats underwent an early insulin replacement (three days after the STZ administration), and another subset underwent a late insulin replacement (twenty days after the STZ administration). Animals were euthanized at 60 and/or 80 days of age. The ventral prostatic lobe was processed for picrosirius red staining, type I and III collagen immunohistochemistry, and gelatin zymography. Hyperglycemic animals showed an increased area of collagen fibers in the prostate, which was composed both types of collagens. MMP-2 activity was significantly reduced in the hyperglycemic animals, while MMP-9 activity was very low and showed no alteration. The simultaneous and late insulin administration restored collagen content and MMP-2 activity. In conclusion, puberty is a critical window for prostate maturation and type-1 diabetes-induced hyperglycemia affects the ratio of the prostatic parenchymal and stromal growth, leading to fibrotic tissues by also MMP-2 down regulation. |
id |
UNSP_4e5491e225561d3baf5bb8def71ad53a |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/179264 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Hyperglycemic condition during puberty increases collagen fibers deposition in the prostatic stroma and reduces MMP-2 activityCollagenDiabetesHyperglycemiaMetalloproteinaseProstatePubertyPuberty is an important period for the growth and maturation of the male reproductive system, and is also a critical window for endocrine or environmental interference. The physiological levels of circulating insulin and hyperglycemic control are important factors for a normal prostate growth. Hyperglycemia during puberty is reported to retard the growth of the prostate gland, with remarkable effects on the epithelial compartment. Here, we investigated the impact of hyperglycemia along with a simultaneous or late insulin replacement on the ventral prostate growth in rats during puberty, paying special attention to the deposition of collagen fibers and activities of gelatinase, matrix metalloproteinase-2 (MMP-2), and −9 (MMP-9). Hyperglycemia was induced by streptozotocin (STZ) administration in 40-day-old male Wistar rats. A subset of hyperglycemic rats underwent an early insulin replacement (three days after the STZ administration), and another subset underwent a late insulin replacement (twenty days after the STZ administration). Animals were euthanized at 60 and/or 80 days of age. The ventral prostatic lobe was processed for picrosirius red staining, type I and III collagen immunohistochemistry, and gelatin zymography. Hyperglycemic animals showed an increased area of collagen fibers in the prostate, which was composed both types of collagens. MMP-2 activity was significantly reduced in the hyperglycemic animals, while MMP-9 activity was very low and showed no alteration. The simultaneous and late insulin administration restored collagen content and MMP-2 activity. In conclusion, puberty is a critical window for prostate maturation and type-1 diabetes-induced hyperglycemia affects the ratio of the prostatic parenchymal and stromal growth, leading to fibrotic tissues by also MMP-2 down regulation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Department of Morphology Institute of Biosciences Sao Paulo State University (UNESP)Center of Biological and Health Sciences (CCBS) State University of West Paraná (UNIOESTE)Department of Morphological Sciences Biological Sciences Center State University of Maringa (UEM)Department of Morphology Institute of Biosciences Sao Paulo State University (UNESP)FAPESP: 06/60116-9FAPESP: 2006/60115-2CNPq: 305391/2014-3Universidade Estadual Paulista (Unesp)State University of West Paraná (UNIOESTE)Universidade Estadual de Maringá (UEM)Santos, Sérgio Alexandre Alcantara dos [UNESP]Porto Amorim, Elaine ManoelaRibeiro, Larissa Mayume [UNESP]Rinaldi, Jaqueline CarvalhoDelella, Flávia KarinaJustulin, Luis Antonio [UNESP]Felisbino, Sérgio Luis [UNESP]2018-12-11T17:34:26Z2018-12-11T17:34:26Z2017-12-02info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1581-1586application/pdfhttp://dx.doi.org/10.1016/j.bbrc.2017.10.022Biochemical and Biophysical Research Communications, v. 493, n. 4, p. 1581-1586, 2017.1090-21040006-291Xhttp://hdl.handle.net/11449/17926410.1016/j.bbrc.2017.10.0222-s2.0-850308343072-s2.0-85030834307.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiochemical and Biophysical Research Communications1,087info:eu-repo/semantics/openAccess2023-11-28T06:18:14Zoai:repositorio.unesp.br:11449/179264Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-28T06:18:14Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hyperglycemic condition during puberty increases collagen fibers deposition in the prostatic stroma and reduces MMP-2 activity |
title |
Hyperglycemic condition during puberty increases collagen fibers deposition in the prostatic stroma and reduces MMP-2 activity |
spellingShingle |
Hyperglycemic condition during puberty increases collagen fibers deposition in the prostatic stroma and reduces MMP-2 activity Santos, Sérgio Alexandre Alcantara dos [UNESP] Collagen Diabetes Hyperglycemia Metalloproteinase Prostate Puberty |
title_short |
Hyperglycemic condition during puberty increases collagen fibers deposition in the prostatic stroma and reduces MMP-2 activity |
title_full |
Hyperglycemic condition during puberty increases collagen fibers deposition in the prostatic stroma and reduces MMP-2 activity |
title_fullStr |
Hyperglycemic condition during puberty increases collagen fibers deposition in the prostatic stroma and reduces MMP-2 activity |
title_full_unstemmed |
Hyperglycemic condition during puberty increases collagen fibers deposition in the prostatic stroma and reduces MMP-2 activity |
title_sort |
Hyperglycemic condition during puberty increases collagen fibers deposition in the prostatic stroma and reduces MMP-2 activity |
author |
Santos, Sérgio Alexandre Alcantara dos [UNESP] |
author_facet |
Santos, Sérgio Alexandre Alcantara dos [UNESP] Porto Amorim, Elaine Manoela Ribeiro, Larissa Mayume [UNESP] Rinaldi, Jaqueline Carvalho Delella, Flávia Karina Justulin, Luis Antonio [UNESP] Felisbino, Sérgio Luis [UNESP] |
author_role |
author |
author2 |
Porto Amorim, Elaine Manoela Ribeiro, Larissa Mayume [UNESP] Rinaldi, Jaqueline Carvalho Delella, Flávia Karina Justulin, Luis Antonio [UNESP] Felisbino, Sérgio Luis [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) State University of West Paraná (UNIOESTE) Universidade Estadual de Maringá (UEM) |
dc.contributor.author.fl_str_mv |
Santos, Sérgio Alexandre Alcantara dos [UNESP] Porto Amorim, Elaine Manoela Ribeiro, Larissa Mayume [UNESP] Rinaldi, Jaqueline Carvalho Delella, Flávia Karina Justulin, Luis Antonio [UNESP] Felisbino, Sérgio Luis [UNESP] |
dc.subject.por.fl_str_mv |
Collagen Diabetes Hyperglycemia Metalloproteinase Prostate Puberty |
topic |
Collagen Diabetes Hyperglycemia Metalloproteinase Prostate Puberty |
description |
Puberty is an important period for the growth and maturation of the male reproductive system, and is also a critical window for endocrine or environmental interference. The physiological levels of circulating insulin and hyperglycemic control are important factors for a normal prostate growth. Hyperglycemia during puberty is reported to retard the growth of the prostate gland, with remarkable effects on the epithelial compartment. Here, we investigated the impact of hyperglycemia along with a simultaneous or late insulin replacement on the ventral prostate growth in rats during puberty, paying special attention to the deposition of collagen fibers and activities of gelatinase, matrix metalloproteinase-2 (MMP-2), and −9 (MMP-9). Hyperglycemia was induced by streptozotocin (STZ) administration in 40-day-old male Wistar rats. A subset of hyperglycemic rats underwent an early insulin replacement (three days after the STZ administration), and another subset underwent a late insulin replacement (twenty days after the STZ administration). Animals were euthanized at 60 and/or 80 days of age. The ventral prostatic lobe was processed for picrosirius red staining, type I and III collagen immunohistochemistry, and gelatin zymography. Hyperglycemic animals showed an increased area of collagen fibers in the prostate, which was composed both types of collagens. MMP-2 activity was significantly reduced in the hyperglycemic animals, while MMP-9 activity was very low and showed no alteration. The simultaneous and late insulin administration restored collagen content and MMP-2 activity. In conclusion, puberty is a critical window for prostate maturation and type-1 diabetes-induced hyperglycemia affects the ratio of the prostatic parenchymal and stromal growth, leading to fibrotic tissues by also MMP-2 down regulation. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-12-02 2018-12-11T17:34:26Z 2018-12-11T17:34:26Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bbrc.2017.10.022 Biochemical and Biophysical Research Communications, v. 493, n. 4, p. 1581-1586, 2017. 1090-2104 0006-291X http://hdl.handle.net/11449/179264 10.1016/j.bbrc.2017.10.022 2-s2.0-85030834307 2-s2.0-85030834307.pdf |
url |
http://dx.doi.org/10.1016/j.bbrc.2017.10.022 http://hdl.handle.net/11449/179264 |
identifier_str_mv |
Biochemical and Biophysical Research Communications, v. 493, n. 4, p. 1581-1586, 2017. 1090-2104 0006-291X 10.1016/j.bbrc.2017.10.022 2-s2.0-85030834307 2-s2.0-85030834307.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biochemical and Biophysical Research Communications 1,087 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1581-1586 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965102598782976 |