Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst
Autor(a) principal: | |
---|---|
Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1471-213X-12-33 http://hdl.handle.net/11449/14679 |
Resumo: | Background: The first distinct differentiation event in mammals occurs at the blastocyst stage when totipotent blastomeres differentiate into either pluripotent inner cell mass (ICM) or multipotent trophectoderm (TE). Here we determined, for the first time, global gene expression patterns in the ICM and TE isolated from bovine blastocysts. The ICM and TE were isolated from blastocysts harvested at day 8 after insemination by magnetic activated cell sorting, and cDNA sequenced using the SOLiD 4.0 system.Results: A total of 870 genes were differentially expressed between ICM and TE. Several genes characteristic of ICM (for example, NANOG, SOX2, and STAT3) and TE (ELF5, GATA3, and KRT18) in mouse and human showed similar patterns in bovine. Other genes, however, showed differences in expression between ICM and TE that deviates from the expected based on mouse and human.Conclusion: Analysis of gene expression indicated that differentiation of blastomeres of the morula-stage embryo into the ICM and TE of the blastocyst is accompanied by differences between the two cell lineages in expression of genes controlling metabolic processes, endocytosis, hatching from the zona pellucida, paracrine and endocrine signaling with the mother, and genes supporting the changes in cellular architecture, stemness, and hematopoiesis necessary for development of the trophoblast. |
id |
UNSP_4ede8c8c9ad8e8bfba60205c3508abb0 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/14679 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Global gene expression of the inner cell mass and trophectoderm of the bovine blastocystBlastocystTrophectodermInner cell massDevelopmentBackground: The first distinct differentiation event in mammals occurs at the blastocyst stage when totipotent blastomeres differentiate into either pluripotent inner cell mass (ICM) or multipotent trophectoderm (TE). Here we determined, for the first time, global gene expression patterns in the ICM and TE isolated from bovine blastocysts. The ICM and TE were isolated from blastocysts harvested at day 8 after insemination by magnetic activated cell sorting, and cDNA sequenced using the SOLiD 4.0 system.Results: A total of 870 genes were differentially expressed between ICM and TE. Several genes characteristic of ICM (for example, NANOG, SOX2, and STAT3) and TE (ELF5, GATA3, and KRT18) in mouse and human showed similar patterns in bovine. Other genes, however, showed differences in expression between ICM and TE that deviates from the expected based on mouse and human.Conclusion: Analysis of gene expression indicated that differentiation of blastomeres of the morula-stage embryo into the ICM and TE of the blastocyst is accompanied by differences between the two cell lineages in expression of genes controlling metabolic processes, endocytosis, hatching from the zona pellucida, paracrine and endocrine signaling with the mother, and genes supporting the changes in cellular architecture, stemness, and hematopoiesis necessary for development of the trophoblast.Agriculture and Food Research Initiative from United States Dept. of Agriculture National Institute of Food and AgricultureResearch Opportunity Fund, University of Florida Research FoundationUniv Florida, Dept Anim Sci, Gainesville, FL 32611 USADH Barron Reprod & Perinatal Biol Res Program, Gainesville, FL 32611 USAUniv Florida, Interdisciplinary Ctr Biotechnol Res, Gainesville, FL 32611 USANatl Agr & Food Res Org, Kyushu Okinawa Agr Res Ctr, Kumamoto, JapanUniv Tokyo, Inst Med Sci, Lab Dev Genet, Tokyo, JapanUniv Tokyo, Inst Med Sci, Ctr Human Genome, Tokyo, JapanUNESP, Fac Med Vet & Zootecn, Dept Reprod Anim & Radiol Vet, Botucatu, SP, BrazilUNESP, Fac Med Vet & Zootecn, Dept Reprod Anim & Radiol Vet, Botucatu, SP, BrazilNIFA: 2009-65203-05732NIFA: 2011-67015-30688Biomed Central Ltd.Univ FloridaDH Barron Reprod & Perinatal Biol Res ProgramNatl Agr & Food Res OrgUniv TokyoUniversidade Estadual Paulista (Unesp)Ozawa, ManabuSakatani, MikiYao, JiQiangShanker, SavitaYu, FahongYamashita, RuiWakabayashi, ShunichiNakai, KentaDobbs, Kyle B.Sudano, Mateus Jose [UNESP]Farmerie, William G.Hansen, Peter J.2013-09-30T18:28:57Z2014-05-20T13:42:13Z2013-09-30T18:28:57Z2014-05-20T13:42:13Z2012-11-06info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-13application/pdfhttp://dx.doi.org/10.1186/1471-213X-12-33Bmc Developmental Biology. London: Biomed Central Ltd., v. 12, p. 1-13, 2012.1471-213Xhttp://hdl.handle.net/11449/1467910.1186/1471-213X-12-33WOS:000312128300001WOS000312128300001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBmc Developmental Biology2.427info:eu-repo/semantics/openAccess2024-09-09T14:00:47Zoai:repositorio.unesp.br:11449/14679Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:00:47Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst |
title |
Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst |
spellingShingle |
Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst Ozawa, Manabu Blastocyst Trophectoderm Inner cell mass Development |
title_short |
Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst |
title_full |
Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst |
title_fullStr |
Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst |
title_full_unstemmed |
Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst |
title_sort |
Global gene expression of the inner cell mass and trophectoderm of the bovine blastocyst |
author |
Ozawa, Manabu |
author_facet |
Ozawa, Manabu Sakatani, Miki Yao, JiQiang Shanker, Savita Yu, Fahong Yamashita, Rui Wakabayashi, Shunichi Nakai, Kenta Dobbs, Kyle B. Sudano, Mateus Jose [UNESP] Farmerie, William G. Hansen, Peter J. |
author_role |
author |
author2 |
Sakatani, Miki Yao, JiQiang Shanker, Savita Yu, Fahong Yamashita, Rui Wakabayashi, Shunichi Nakai, Kenta Dobbs, Kyle B. Sudano, Mateus Jose [UNESP] Farmerie, William G. Hansen, Peter J. |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Univ Florida DH Barron Reprod & Perinatal Biol Res Program Natl Agr & Food Res Org Univ Tokyo Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Ozawa, Manabu Sakatani, Miki Yao, JiQiang Shanker, Savita Yu, Fahong Yamashita, Rui Wakabayashi, Shunichi Nakai, Kenta Dobbs, Kyle B. Sudano, Mateus Jose [UNESP] Farmerie, William G. Hansen, Peter J. |
dc.subject.por.fl_str_mv |
Blastocyst Trophectoderm Inner cell mass Development |
topic |
Blastocyst Trophectoderm Inner cell mass Development |
description |
Background: The first distinct differentiation event in mammals occurs at the blastocyst stage when totipotent blastomeres differentiate into either pluripotent inner cell mass (ICM) or multipotent trophectoderm (TE). Here we determined, for the first time, global gene expression patterns in the ICM and TE isolated from bovine blastocysts. The ICM and TE were isolated from blastocysts harvested at day 8 after insemination by magnetic activated cell sorting, and cDNA sequenced using the SOLiD 4.0 system.Results: A total of 870 genes were differentially expressed between ICM and TE. Several genes characteristic of ICM (for example, NANOG, SOX2, and STAT3) and TE (ELF5, GATA3, and KRT18) in mouse and human showed similar patterns in bovine. Other genes, however, showed differences in expression between ICM and TE that deviates from the expected based on mouse and human.Conclusion: Analysis of gene expression indicated that differentiation of blastomeres of the morula-stage embryo into the ICM and TE of the blastocyst is accompanied by differences between the two cell lineages in expression of genes controlling metabolic processes, endocytosis, hatching from the zona pellucida, paracrine and endocrine signaling with the mother, and genes supporting the changes in cellular architecture, stemness, and hematopoiesis necessary for development of the trophoblast. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-11-06 2013-09-30T18:28:57Z 2013-09-30T18:28:57Z 2014-05-20T13:42:13Z 2014-05-20T13:42:13Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1471-213X-12-33 Bmc Developmental Biology. London: Biomed Central Ltd., v. 12, p. 1-13, 2012. 1471-213X http://hdl.handle.net/11449/14679 10.1186/1471-213X-12-33 WOS:000312128300001 WOS000312128300001.pdf |
url |
http://dx.doi.org/10.1186/1471-213X-12-33 http://hdl.handle.net/11449/14679 |
identifier_str_mv |
Bmc Developmental Biology. London: Biomed Central Ltd., v. 12, p. 1-13, 2012. 1471-213X 10.1186/1471-213X-12-33 WOS:000312128300001 WOS000312128300001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Bmc Developmental Biology 2.427 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-13 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1813546561015119872 |