Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH

Detalhes bibliográficos
Autor(a) principal: Andrade, Evelyn Rabelo
Data de Publicação: 2011
Outros Autores: Maddox-Hyttel, Poul, Landim-Alvarenga, Fernanda Da Cruz [UNESP], Viana Silva, José Roberto, Alfieri, Amauri Alcindo, Seneda, Marcelo Marcondes, Figueiredo, José Ricardo, Toniolli, Ricardo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.4061/2011/670987
http://hdl.handle.net/11449/72915
Resumo: The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH. To evaluate ultrastructure of primordial follicles cultured in MEM (control) or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy. Except in the control, primordial follicles cultured in supplemented media for 6d were ultrastructurally normal. They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed. Rough endoplasmic reticulum (RER) was rarely found. The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER. In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro. © 2011 Evelyn Rabelo Andrade et al.
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spelling Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSHOvis ariesThe aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH. To evaluate ultrastructure of primordial follicles cultured in MEM (control) or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy. Except in the control, primordial follicles cultured in supplemented media for 6d were ultrastructurally normal. They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed. Rough endoplasmic reticulum (RER) was rarely found. The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER. In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro. © 2011 Evelyn Rabelo Andrade et al.Universidade Estadual de Londrina DCV-CCA-UEL, Londrina, PR 86051-990University of Copenhagen, Groennegaardsvej 7, 1870 Frederiksberg CDepartamento de Reprodução Animal Universidade Estadual de São Paulo, Botucatu, SP 18610-307Universidade Federal Do Ceará, Sobral, CE 60020-181Medicina Veterinária Universidade Estadual Do Ceará, Fortaleza, CE 60740-000Departamento de Reprodução Animal Universidade Estadual de São Paulo, Botucatu, SP 18610-307Universidade Estadual de Londrina (UEL)University of CopenhagenUniversidade Estadual Paulista (Unesp)Universidade Federal do Ceará (UFC)Universidade Estadual do Ceará (UECE)Andrade, Evelyn RabeloMaddox-Hyttel, PoulLandim-Alvarenga, Fernanda Da Cruz [UNESP]Viana Silva, José RobertoAlfieri, Amauri AlcindoSeneda, Marcelo MarcondesFigueiredo, José RicardoToniolli, Ricardo2014-05-27T11:26:16Z2014-05-27T11:26:16Z2011-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.4061/2011/670987Veterinary Medicine International, v. 2011.2042-0048http://hdl.handle.net/11449/7291510.4061/2011/6709872-s2.0-806551242112-s2.0-80655124211.pdf84564903008148330000-0002-2420-2550Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVeterinary Medicine International0,536info:eu-repo/semantics/openAccess2024-09-09T14:00:56Zoai:repositorio.unesp.br:11449/72915Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-09T14:00:56Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH
title Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH
spellingShingle Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH
Andrade, Evelyn Rabelo
Ovis aries
title_short Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH
title_full Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH
title_fullStr Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH
title_full_unstemmed Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH
title_sort Ultrastructure of sheep primordial follicles cultured in the presence of indol acetic acid, EGF, and FSH
author Andrade, Evelyn Rabelo
author_facet Andrade, Evelyn Rabelo
Maddox-Hyttel, Poul
Landim-Alvarenga, Fernanda Da Cruz [UNESP]
Viana Silva, José Roberto
Alfieri, Amauri Alcindo
Seneda, Marcelo Marcondes
Figueiredo, José Ricardo
Toniolli, Ricardo
author_role author
author2 Maddox-Hyttel, Poul
Landim-Alvarenga, Fernanda Da Cruz [UNESP]
Viana Silva, José Roberto
Alfieri, Amauri Alcindo
Seneda, Marcelo Marcondes
Figueiredo, José Ricardo
Toniolli, Ricardo
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Londrina (UEL)
University of Copenhagen
Universidade Estadual Paulista (Unesp)
Universidade Federal do Ceará (UFC)
Universidade Estadual do Ceará (UECE)
dc.contributor.author.fl_str_mv Andrade, Evelyn Rabelo
Maddox-Hyttel, Poul
Landim-Alvarenga, Fernanda Da Cruz [UNESP]
Viana Silva, José Roberto
Alfieri, Amauri Alcindo
Seneda, Marcelo Marcondes
Figueiredo, José Ricardo
Toniolli, Ricardo
dc.subject.por.fl_str_mv Ovis aries
topic Ovis aries
description The aim of this study was to investigate the ultrastructural characteristics of primordial follicles after culturing of sheep ovarian cortical slices in the presence of indol acetic acid (IAA), Epidermal Growth Factor (EGF), and FSH. To evaluate ultrastructure of primordial follicles cultured in MEM (control) or in MEM containing IAA, EGF, and FSH, fragments of cultured tissue were processes for transmission electron microscopy. Except in the control, primordial follicles cultured in supplemented media for 6d were ultrastructurally normal. They had oocyte with intact nucleus and the cytoplasm contained heterogeneous-sized lipid droplets and numerous round or elongated mitochondria with intact parallel cristae were observed. Rough endoplasmic reticulum (RER) was rarely found. The granulosa cells cytoplasm contained a great number of mitochondria and abundant RER. In conclusion, the presence of IAA, EGF, and FSH helped to maintain ultrastructural integrity of sheep primordial follicles cultured in vitro. © 2011 Evelyn Rabelo Andrade et al.
publishDate 2011
dc.date.none.fl_str_mv 2011-12-01
2014-05-27T11:26:16Z
2014-05-27T11:26:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.4061/2011/670987
Veterinary Medicine International, v. 2011.
2042-0048
http://hdl.handle.net/11449/72915
10.4061/2011/670987
2-s2.0-80655124211
2-s2.0-80655124211.pdf
8456490300814833
0000-0002-2420-2550
url http://dx.doi.org/10.4061/2011/670987
http://hdl.handle.net/11449/72915
identifier_str_mv Veterinary Medicine International, v. 2011.
2042-0048
10.4061/2011/670987
2-s2.0-80655124211
2-s2.0-80655124211.pdf
8456490300814833
0000-0002-2420-2550
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Veterinary Medicine International
0,536
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1813546540532236288

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