MIB-1 Is a Suitable Marker of Proliferative Activity in Formalin-fixed, Paraffin-embedded Sections of Breast Cancer
Autor(a) principal: | |
---|---|
Data de Publicação: | 1995 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1177/106689699500200405 http://hdl.handle.net/11449/219179 |
Resumo: | To investigate the expression of a marker of cell proliferation (MIB-1) and its puta tive relationship with tumor size, histologic grading, mitotic index, nodal status, estrogen receptor content, p53 immunoreactivity, and ploidy, we studied 40 cases of invasive breast carcinoma in formalin-fixed, paraffin-embedded tissue sections. The MIB-1, estrogen receptor, and p53 were detected using an avidin-biotin-peroxidase method with microwave antigen retrieval. Ploidy was assessed by image analysis. The median value of MIB-1 index was 15.9% and ranged from 5% to 90%. We found a significant and positive relationship among the MIB-1 index and histologic grading, mitotic index, p53 immunoreactivity, and ploidy, and we demonstrated an inverse relationship between the MIB-1 index and estrogen receptor content. These “ results are similar to those reported using Ki-67 in frozen sections from breast car cinomas. Thus, MIB-1 detected in formalin-fixed material with microwave pre treatment looks to be a reliable marker of proliferation in breast carcinomas. Int J Surg Pathol 2(4):287-294, 1995. © 1995, Sage Publications. All rights reserved. |
id |
UNSP_4fb5d5bd712b57e3ec6e150b6bf4c64f |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/219179 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
MIB-1 Is a Suitable Marker of Proliferative Activity in Formalin-fixed, Paraffin-embedded Sections of Breast Cancerbreast cancerKi-67 antigenMIB-1To investigate the expression of a marker of cell proliferation (MIB-1) and its puta tive relationship with tumor size, histologic grading, mitotic index, nodal status, estrogen receptor content, p53 immunoreactivity, and ploidy, we studied 40 cases of invasive breast carcinoma in formalin-fixed, paraffin-embedded tissue sections. The MIB-1, estrogen receptor, and p53 were detected using an avidin-biotin-peroxidase method with microwave antigen retrieval. Ploidy was assessed by image analysis. The median value of MIB-1 index was 15.9% and ranged from 5% to 90%. We found a significant and positive relationship among the MIB-1 index and histologic grading, mitotic index, p53 immunoreactivity, and ploidy, and we demonstrated an inverse relationship between the MIB-1 index and estrogen receptor content. These “ results are similar to those reported using Ki-67 in frozen sections from breast car cinomas. Thus, MIB-1 detected in formalin-fixed material with microwave pre treatment looks to be a reliable marker of proliferation in breast carcinomas. Int J Surg Pathol 2(4):287-294, 1995. © 1995, Sage Publications. All rights reserved.Botucatu School of Medicine UNESP, Botucatu, São PauloSchool of Medicine Federal University of CearáBotucatu School of Medicine UNESP, Botucatu, São PauloUniversidade Estadual Paulista (UNESP)Federal University of CearáSchmitt, Fernando Carlos [UNESP]Ferreira, Marcia Pitombeira2022-04-28T18:54:07Z2022-04-28T18:54:07Z1995-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article287-293http://dx.doi.org/10.1177/106689699500200405International Journal of Surgical Pathology, v. 2, n. 4, p. 287-293, 1995.1940-24651066-8969http://hdl.handle.net/11449/21917910.1177/1066896995002004052-s2.0-0028947727Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Surgical Pathologyinfo:eu-repo/semantics/openAccess2022-04-28T18:54:07Zoai:repositorio.unesp.br:11449/219179Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462022-04-28T18:54:07Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
MIB-1 Is a Suitable Marker of Proliferative Activity in Formalin-fixed, Paraffin-embedded Sections of Breast Cancer |
title |
MIB-1 Is a Suitable Marker of Proliferative Activity in Formalin-fixed, Paraffin-embedded Sections of Breast Cancer |
spellingShingle |
MIB-1 Is a Suitable Marker of Proliferative Activity in Formalin-fixed, Paraffin-embedded Sections of Breast Cancer Schmitt, Fernando Carlos [UNESP] breast cancer Ki-67 antigen MIB-1 |
title_short |
MIB-1 Is a Suitable Marker of Proliferative Activity in Formalin-fixed, Paraffin-embedded Sections of Breast Cancer |
title_full |
MIB-1 Is a Suitable Marker of Proliferative Activity in Formalin-fixed, Paraffin-embedded Sections of Breast Cancer |
title_fullStr |
MIB-1 Is a Suitable Marker of Proliferative Activity in Formalin-fixed, Paraffin-embedded Sections of Breast Cancer |
title_full_unstemmed |
MIB-1 Is a Suitable Marker of Proliferative Activity in Formalin-fixed, Paraffin-embedded Sections of Breast Cancer |
title_sort |
MIB-1 Is a Suitable Marker of Proliferative Activity in Formalin-fixed, Paraffin-embedded Sections of Breast Cancer |
author |
Schmitt, Fernando Carlos [UNESP] |
author_facet |
Schmitt, Fernando Carlos [UNESP] Ferreira, Marcia Pitombeira |
author_role |
author |
author2 |
Ferreira, Marcia Pitombeira |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Federal University of Ceará |
dc.contributor.author.fl_str_mv |
Schmitt, Fernando Carlos [UNESP] Ferreira, Marcia Pitombeira |
dc.subject.por.fl_str_mv |
breast cancer Ki-67 antigen MIB-1 |
topic |
breast cancer Ki-67 antigen MIB-1 |
description |
To investigate the expression of a marker of cell proliferation (MIB-1) and its puta tive relationship with tumor size, histologic grading, mitotic index, nodal status, estrogen receptor content, p53 immunoreactivity, and ploidy, we studied 40 cases of invasive breast carcinoma in formalin-fixed, paraffin-embedded tissue sections. The MIB-1, estrogen receptor, and p53 were detected using an avidin-biotin-peroxidase method with microwave antigen retrieval. Ploidy was assessed by image analysis. The median value of MIB-1 index was 15.9% and ranged from 5% to 90%. We found a significant and positive relationship among the MIB-1 index and histologic grading, mitotic index, p53 immunoreactivity, and ploidy, and we demonstrated an inverse relationship between the MIB-1 index and estrogen receptor content. These “ results are similar to those reported using Ki-67 in frozen sections from breast car cinomas. Thus, MIB-1 detected in formalin-fixed material with microwave pre treatment looks to be a reliable marker of proliferation in breast carcinomas. Int J Surg Pathol 2(4):287-294, 1995. © 1995, Sage Publications. All rights reserved. |
publishDate |
1995 |
dc.date.none.fl_str_mv |
1995-01-01 2022-04-28T18:54:07Z 2022-04-28T18:54:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1177/106689699500200405 International Journal of Surgical Pathology, v. 2, n. 4, p. 287-293, 1995. 1940-2465 1066-8969 http://hdl.handle.net/11449/219179 10.1177/106689699500200405 2-s2.0-0028947727 |
url |
http://dx.doi.org/10.1177/106689699500200405 http://hdl.handle.net/11449/219179 |
identifier_str_mv |
International Journal of Surgical Pathology, v. 2, n. 4, p. 287-293, 1995. 1940-2465 1066-8969 10.1177/106689699500200405 2-s2.0-0028947727 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Surgical Pathology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
287-293 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964491333500928 |