Ação do ácido zoledrônico e terapia estrogênica na regeneração óssea alveolar de ratas senis

• Autor(a) principal: Momesso, Gustavo Antonio Correa [UNESP]
• Data de Publicação: 2014
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149558; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-27/000866572.pdf
• topic: Estrogênios; Envelhecimento; Regeneração óssea; Estrogens

Bisphosphonates, pyrophosphates endogenous analogs are drugs used to treat various metabolic bone diseases such as osteoporosis. Osteonecrosis of the jaw is emerging complication of bisphosphonate therapy and is associated with significant morbidity. Our hypothesis is that hormone replacement therapy with estradiol on acyclic senile rats (18-20 months) could attenuate the development of osteonecrosis of the jaw after 8 weeks of treatment with zoledronic acid and extraction of the lower first molar of animals with the aim to evaluate the performance of ZOL in the alveolar bone regeneration in Wistar rats submitted or not senile estrogen therapy process. Biochemical analyzes to verify the plasma concentration of phosphorus, alkaline phosphatase were performed; histology and immunohistochemistry to evaluate the process of alveolar repair of animals 28 days after the extraction of the tooth through the histological slides and marking of TRAP / RANKL / OPG protein analysis. The results obtained in relation to plasma level showed no significant difference in the plasma concentration of calcium and phosphorus between the experimental groups. However, the enzymatic activity of alkaline phosphatase was lower in the group of animals E2 / NaCl. The results of the histological analysis showed that the OM / ZOL and E2 / ZOL groups exhibited osteonecrosis with impaired alveolar repair and that E2 failed to ease the situation. Already OM / NaCl and E2 / NaCl groups exhibited good bone formation and epithelialization of the alveolar mucosa, presenting bone vitality, unlike the groups treated with zoledronic acid. The results related to the immunohistochemical staining showed that the OM / NaCl group there was a balance in marking proteins and that the OM / ZOL group, there was suppression in marking these. Already groups receiving estradiol...