A ação da genisteína no reparo ósseo alveolar e remodelação do colo do fêmur de ratas ovariectomizadas

• Autor(a) principal: Moreira, Luiza Tavares [UNESP]
• Data de Publicação: 2017
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/156692; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2018-06-12/000901552.pdf
• topic: Estrogênios; Genisteina; Extração dentária; Ovariectomia; Estrogens

In mandibular and maxillary bones, hypoestrogenism affects the alveolar repair process, reducing bone formation after tooth extraction. And in the process of remodeling the bone tissue provides progressive decrease of bone mass. Among the drugs studied in the attempt to minimize these effects, soy isoflavones, such as genistein (Gen), are considered natural modulators of the estrogen receptor because of their conformational binding. However, its action on bone tissue remains controversial. The objective of this study was to analyze the action of Gen in alveolar bone repair and remodeling of the femoral neck in ovariectomized rats (OVX). Fourty Wistar rats (6 months) were randomly assigned to the following groups: Sham/NaCl; Sham/Gen; OVX/NaCl; OVX/Gen. The vaginal smear of the animals was collected daily to verify the estrous cycle for 15 days and only animals with regular estrous cycle were accepted for our study. Subsequently, the rats of the OVX groups at the 60 month were OVX, and the rats of the Sham groups underwent the ovarian removal mock surgery. Ten days after surgeries, the Sham/Gen and OVX/Gen experimental groups received 5.76 mg/kg/day of Gen, and the Sham/NaCl and OVX/NaCl groups received 0.3 mL of NaCl by gavage during 90 days. The right upper central incisor was extracted after 62 days of substitutive therapy. At the end of the 90 days, they were sacrificed, the right alveolus was removed and the left femur was collected. The alveolar middle third region was analyzed by histometric analysis and immunohistochemical analysis of protein immunolabeling, Tartarate Resistant Acid Phosphatase (TRAP) and Osteocalcin (OCN). The femoral neck was analyzed using the computerized microtomograph. In alveolar bone repair the effects of ovariectomy were observed, with a significant increase in the immunoblot of the TRAP, but there was...