Formação de biofilme cristalino de Proteus mirabilis em cateter urológico

Detalhes bibliográficos
Autor(a) principal: dos Santos, Cássio Antonio Lanfredi [UNESP]
Data de Publicação: 2010
Outros Autores: de Campos Júnior, Flávio Ferraz, Pizzolitto, Antonio Carlos [UNESP], Pizzolitto, Elisabeth Loshchagin [UNESP]
Tipo de documento: Artigo
Idioma: eng
por
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://www.rbeb.org.br/articles/view/id/4dfa530418626adbef000008
http://hdl.handle.net/11449/72207
Resumo: The indwelling urethral catheter has an important role for patients with urinary retention, bladder obstruction, neurological damage and other diseases. Urine contains minerals which precipitate in alkaline pH, crystallize and block the urological catheter. The crystallization of the ionic components of urine occurs in the presence of urease, an enzyme produced by Proteus mirabilis. This bacterium adheres to inanimate surfaces and forms biofilms. The aim of this study was to investigate the formation of crystalline biofilm on the luminal surface of siliconized latex catheters by means of scanning electron microscope, after channeling artificial urine infected with Proteus mirabilis. The experiment was performed in vitro using a dynamic flow system. The artificial urine compounds were salts of calcium, magnesium, phosphates, urea and egg albumin, and it was infected with Proteus mirabilis ATCC 25933. The urine flow was stopped after crystallization of the ionic components. Crystallization was observed after alkalinization of urine. Scanning electron microscopy showed the presence of crystals and morphologies typical of bacilli embedded in an amorphous mass on the internal lumen of the catheter. The present study showed that catheter encrustation may limit the use of long-term indwelling catheter. © SBEB - Sociedade Brasileira de Engenharia Biomédica.
id UNSP_511077342d6d1344b13eca0e2d58c228
oai_identifier_str oai:repositorio.unesp.br:11449/72207
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling Formação de biofilme cristalino de Proteus mirabilis em cateter urológicoFormation of crystalline biofilm of proteus mirabilis in urological catheterArtificial urineCrystalline biofilmEncrustation of cathetersFoley catheterProteus mirabilisBiofilmsBiofiltersBody fluidsCrystalline materialsCrystallizationMagnesiumScanning electron microscopyUreaCathetersThe indwelling urethral catheter has an important role for patients with urinary retention, bladder obstruction, neurological damage and other diseases. Urine contains minerals which precipitate in alkaline pH, crystallize and block the urological catheter. The crystallization of the ionic components of urine occurs in the presence of urease, an enzyme produced by Proteus mirabilis. This bacterium adheres to inanimate surfaces and forms biofilms. The aim of this study was to investigate the formation of crystalline biofilm on the luminal surface of siliconized latex catheters by means of scanning electron microscope, after channeling artificial urine infected with Proteus mirabilis. The experiment was performed in vitro using a dynamic flow system. The artificial urine compounds were salts of calcium, magnesium, phosphates, urea and egg albumin, and it was infected with Proteus mirabilis ATCC 25933. The urine flow was stopped after crystallization of the ionic components. Crystallization was observed after alkalinization of urine. Scanning electron microscopy showed the presence of crystals and morphologies typical of bacilli embedded in an amorphous mass on the internal lumen of the catheter. The present study showed that catheter encrustation may limit the use of long-term indwelling catheter. © SBEB - Sociedade Brasileira de Engenharia Biomédica.Faculdade de Ciências Farmacêuticas, UNESPPrograma de Pós-Graduação Interunidades em Bioengenharia / USP-São CarlosFaculdade de Ciências Farmacêuticas, UNESP, Rua Expedicionários do Brasil, 1621, 14801-360 Araraquara, SPFaculdade de Ciências Farmacêuticas, UNESPFaculdade de Ciências Farmacêuticas, UNESP, Rua Expedicionários do Brasil, 1621, 14801-360 Araraquara, SPUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)dos Santos, Cássio Antonio Lanfredi [UNESP]de Campos Júnior, Flávio FerrazPizzolitto, Antonio Carlos [UNESP]Pizzolitto, Elisabeth Loshchagin [UNESP]2014-05-27T11:25:25Z2014-05-27T11:25:25Z2010-12-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article91-98application/pdfhttp://www.rbeb.org.br/articles/view/id/4dfa530418626adbef000008Revista Brasileira de Engenharia Biomedica, v. 26, n. 2, p. 91-98, 2010.1517-31511984-7742http://hdl.handle.net/11449/722072-s2.0-786515318152-s2.0-78651531815.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengporRevista Brasileira de Engenharia Biomédica0,179info:eu-repo/semantics/openAccess2024-06-21T15:19:09Zoai:repositorio.unesp.br:11449/72207Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-06-21T15:19:09Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Formação de biofilme cristalino de Proteus mirabilis em cateter urológico
Formation of crystalline biofilm of proteus mirabilis in urological catheter
title Formação de biofilme cristalino de Proteus mirabilis em cateter urológico
spellingShingle Formação de biofilme cristalino de Proteus mirabilis em cateter urológico
dos Santos, Cássio Antonio Lanfredi [UNESP]
Artificial urine
Crystalline biofilm
Encrustation of catheters
Foley catheter
Proteus mirabilis
Biofilms
Biofilters
Body fluids
Crystalline materials
Crystallization
Magnesium
Scanning electron microscopy
Urea
Catheters
title_short Formação de biofilme cristalino de Proteus mirabilis em cateter urológico
title_full Formação de biofilme cristalino de Proteus mirabilis em cateter urológico
title_fullStr Formação de biofilme cristalino de Proteus mirabilis em cateter urológico
title_full_unstemmed Formação de biofilme cristalino de Proteus mirabilis em cateter urológico
title_sort Formação de biofilme cristalino de Proteus mirabilis em cateter urológico
author dos Santos, Cássio Antonio Lanfredi [UNESP]
author_facet dos Santos, Cássio Antonio Lanfredi [UNESP]
de Campos Júnior, Flávio Ferraz
Pizzolitto, Antonio Carlos [UNESP]
Pizzolitto, Elisabeth Loshchagin [UNESP]
author_role author
author2 de Campos Júnior, Flávio Ferraz
Pizzolitto, Antonio Carlos [UNESP]
Pizzolitto, Elisabeth Loshchagin [UNESP]
author2_role author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv dos Santos, Cássio Antonio Lanfredi [UNESP]
de Campos Júnior, Flávio Ferraz
Pizzolitto, Antonio Carlos [UNESP]
Pizzolitto, Elisabeth Loshchagin [UNESP]
dc.subject.por.fl_str_mv Artificial urine
Crystalline biofilm
Encrustation of catheters
Foley catheter
Proteus mirabilis
Biofilms
Biofilters
Body fluids
Crystalline materials
Crystallization
Magnesium
Scanning electron microscopy
Urea
Catheters
topic Artificial urine
Crystalline biofilm
Encrustation of catheters
Foley catheter
Proteus mirabilis
Biofilms
Biofilters
Body fluids
Crystalline materials
Crystallization
Magnesium
Scanning electron microscopy
Urea
Catheters
description The indwelling urethral catheter has an important role for patients with urinary retention, bladder obstruction, neurological damage and other diseases. Urine contains minerals which precipitate in alkaline pH, crystallize and block the urological catheter. The crystallization of the ionic components of urine occurs in the presence of urease, an enzyme produced by Proteus mirabilis. This bacterium adheres to inanimate surfaces and forms biofilms. The aim of this study was to investigate the formation of crystalline biofilm on the luminal surface of siliconized latex catheters by means of scanning electron microscope, after channeling artificial urine infected with Proteus mirabilis. The experiment was performed in vitro using a dynamic flow system. The artificial urine compounds were salts of calcium, magnesium, phosphates, urea and egg albumin, and it was infected with Proteus mirabilis ATCC 25933. The urine flow was stopped after crystallization of the ionic components. Crystallization was observed after alkalinization of urine. Scanning electron microscopy showed the presence of crystals and morphologies typical of bacilli embedded in an amorphous mass on the internal lumen of the catheter. The present study showed that catheter encrustation may limit the use of long-term indwelling catheter. © SBEB - Sociedade Brasileira de Engenharia Biomédica.
publishDate 2010
dc.date.none.fl_str_mv 2010-12-03
2014-05-27T11:25:25Z
2014-05-27T11:25:25Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.rbeb.org.br/articles/view/id/4dfa530418626adbef000008
Revista Brasileira de Engenharia Biomedica, v. 26, n. 2, p. 91-98, 2010.
1517-3151
1984-7742
http://hdl.handle.net/11449/72207
2-s2.0-78651531815
2-s2.0-78651531815.pdf
url http://www.rbeb.org.br/articles/view/id/4dfa530418626adbef000008
http://hdl.handle.net/11449/72207
identifier_str_mv Revista Brasileira de Engenharia Biomedica, v. 26, n. 2, p. 91-98, 2010.
1517-3151
1984-7742
2-s2.0-78651531815
2-s2.0-78651531815.pdf
dc.language.iso.fl_str_mv eng
por
language eng
por
dc.relation.none.fl_str_mv Revista Brasileira de Engenharia Biomédica
0,179
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 91-98
application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1803649997659439104

Registros relacionados