Taenia saginata: Polymerase chain reaction for taeniasis diagnosis in human fecal samples

Detalhes bibliográficos
Autor(a) principal: Nunes, Cáris Maroni [UNESP]
Data de Publicação: 2003
Outros Autores: Lima, Luiz Gustave Ferraz [UNESP], Manoel, Camila Santos [UNESP], Pereira, Rodrigo Norberte [UNESP], Nakano, Mauro Massaham [UNESP], Garcia, José Fernando [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/S0014-4894(03)00111-5
http://hdl.handle.net/11449/224362
Resumo: The taeniasis-cysticercosis complex is a zoonosis of great medical and economic importance where humans play an important role as the carrier of adult stage of Taenia solium and Taenia saginata. This paper describes PCR standardization that can be applied in human fecal samples for taeniasis diagnosis. DNA extraction was achieved with DNAzol reagent, after egg disruption with glass beads. DNA prepared from fecal specimens was first purified and PCR amplified generating fragments of 170 and 600 bp. The assay described herein provides an important tool for T. saginata identification in human fecal samples. © 2003 Elsevier Science (USA). All rights reserved.
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spelling Taenia saginata: Polymerase chain reaction for taeniasis diagnosis in human fecal samplesThe taeniasis-cysticercosis complex is a zoonosis of great medical and economic importance where humans play an important role as the carrier of adult stage of Taenia solium and Taenia saginata. This paper describes PCR standardization that can be applied in human fecal samples for taeniasis diagnosis. DNA extraction was achieved with DNAzol reagent, after egg disruption with glass beads. DNA prepared from fecal specimens was first purified and PCR amplified generating fragments of 170 and 600 bp. The assay described herein provides an important tool for T. saginata identification in human fecal samples. © 2003 Elsevier Science (USA). All rights reserved.Department of Animal Health and Production Universidade Estadual Paulista Júlio de Mesquita Filho Campus Aracatuba, Rua Clóvis Pestana, 793, Araçatuba, SP 16050-680Department of Animal Health and Production Universidade Estadual Paulista Júlio de Mesquita Filho Campus Aracatuba, Rua Clóvis Pestana, 793, Araçatuba, SP 16050-680Universidade Estadual Paulista (UNESP)Nunes, Cáris Maroni [UNESP]Lima, Luiz Gustave Ferraz [UNESP]Manoel, Camila Santos [UNESP]Pereira, Rodrigo Norberte [UNESP]Nakano, Mauro Massaham [UNESP]Garcia, José Fernando [UNESP]2022-04-28T19:56:06Z2022-04-28T19:56:06Z2003-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article67-69http://dx.doi.org/10.1016/S0014-4894(03)00111-5Experimental Parasitology, v. 104, n. 1-2, p. 67-69, 2003.1090-24490014-4894http://hdl.handle.net/11449/22436210.1016/S0014-4894(03)00111-52-s2.0-0141669146Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengExperimental Parasitologyinfo:eu-repo/semantics/openAccess2024-09-04T19:15:11Zoai:repositorio.unesp.br:11449/224362Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-04T19:15:11Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Taenia saginata: Polymerase chain reaction for taeniasis diagnosis in human fecal samples
title Taenia saginata: Polymerase chain reaction for taeniasis diagnosis in human fecal samples
spellingShingle Taenia saginata: Polymerase chain reaction for taeniasis diagnosis in human fecal samples
Nunes, Cáris Maroni [UNESP]
title_short Taenia saginata: Polymerase chain reaction for taeniasis diagnosis in human fecal samples
title_full Taenia saginata: Polymerase chain reaction for taeniasis diagnosis in human fecal samples
title_fullStr Taenia saginata: Polymerase chain reaction for taeniasis diagnosis in human fecal samples
title_full_unstemmed Taenia saginata: Polymerase chain reaction for taeniasis diagnosis in human fecal samples
title_sort Taenia saginata: Polymerase chain reaction for taeniasis diagnosis in human fecal samples
author Nunes, Cáris Maroni [UNESP]
author_facet Nunes, Cáris Maroni [UNESP]
Lima, Luiz Gustave Ferraz [UNESP]
Manoel, Camila Santos [UNESP]
Pereira, Rodrigo Norberte [UNESP]
Nakano, Mauro Massaham [UNESP]
Garcia, José Fernando [UNESP]
author_role author
author2 Lima, Luiz Gustave Ferraz [UNESP]
Manoel, Camila Santos [UNESP]
Pereira, Rodrigo Norberte [UNESP]
Nakano, Mauro Massaham [UNESP]
Garcia, José Fernando [UNESP]
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Nunes, Cáris Maroni [UNESP]
Lima, Luiz Gustave Ferraz [UNESP]
Manoel, Camila Santos [UNESP]
Pereira, Rodrigo Norberte [UNESP]
Nakano, Mauro Massaham [UNESP]
Garcia, José Fernando [UNESP]
description The taeniasis-cysticercosis complex is a zoonosis of great medical and economic importance where humans play an important role as the carrier of adult stage of Taenia solium and Taenia saginata. This paper describes PCR standardization that can be applied in human fecal samples for taeniasis diagnosis. DNA extraction was achieved with DNAzol reagent, after egg disruption with glass beads. DNA prepared from fecal specimens was first purified and PCR amplified generating fragments of 170 and 600 bp. The assay described herein provides an important tool for T. saginata identification in human fecal samples. © 2003 Elsevier Science (USA). All rights reserved.
publishDate 2003
dc.date.none.fl_str_mv 2003-01-01
2022-04-28T19:56:06Z
2022-04-28T19:56:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/S0014-4894(03)00111-5
Experimental Parasitology, v. 104, n. 1-2, p. 67-69, 2003.
1090-2449
0014-4894
http://hdl.handle.net/11449/224362
10.1016/S0014-4894(03)00111-5
2-s2.0-0141669146
url http://dx.doi.org/10.1016/S0014-4894(03)00111-5
http://hdl.handle.net/11449/224362
identifier_str_mv Experimental Parasitology, v. 104, n. 1-2, p. 67-69, 2003.
1090-2449
0014-4894
10.1016/S0014-4894(03)00111-5
2-s2.0-0141669146
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Experimental Parasitology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 67-69
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv repositoriounesp@unesp.br
_version_ 1810021371973992448

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