Nanostructured polyelectrolyte complexes based on chitosan and sodium alginate containing rifampicin for the potential treatment of tuberculosis
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/03639045.2022.2048664 http://hdl.handle.net/11449/241087 |
Resumo: | Nanostructured polyelectrolyte complexes (nano PECs) were obtained by polyelectrolyte complexation technique from chitosan (CS) and sodium alginate (SA). Different polymer proportions were tested, as well as the addition order and homogenization type, to assess the influence on the nano PECs characteristics. The spherical shape and nanometric scale of the systems were observed by scanning electron microscopy (SEM). Nano PECs size, PDI, and zeta potential (ZP) ranged from 252 to 616 nm, from 0.22 to 0.73 and −50 to 30 mV, respectively. The increase of polymer proportion and the ultra-turrax homogenization led to the enlargement of particles size and PDI. However, no influence was observed on the ZP. The NP1s-Rb and NP4s-Rb, obtained through the sonicator with rifampicin (RIF) added before the CS and SA complexation, were selected due to the most promising characteristics of diameter (301 and 402 nm), PDI (0.27 and 0.26), and RIF incorporation (78 and 69%). The release profiles of RIF incorporated in both nano PECs were similar, with a sustained release of the drug for 180 min in phosphate buffer pH 7.2. The Weibull and the Korsmeyer–Peppas models better describe the RIF release from NP1s-Rb and NP4s-Rb, respectively, demonstrating that the release process was driven by different mechanism according to the particle composition. The nano PECs were lyophilized to prolong it stability and for possible nebulization. The addition of dextrose to the system allowed for resuspension after lyophilization. Therefore, with the results obtained, the incorporation of RIF in nano PECs based on CS and SA presents a promising system for the treatment of tuberculosis. |
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Nanostructured polyelectrolyte complexes based on chitosan and sodium alginate containing rifampicin for the potential treatment of tuberculosismanufacturing parametersMycobacterium tuberculosispolyelectrolyte complexesrifampicinTuberculosisNanostructured polyelectrolyte complexes (nano PECs) were obtained by polyelectrolyte complexation technique from chitosan (CS) and sodium alginate (SA). Different polymer proportions were tested, as well as the addition order and homogenization type, to assess the influence on the nano PECs characteristics. The spherical shape and nanometric scale of the systems were observed by scanning electron microscopy (SEM). Nano PECs size, PDI, and zeta potential (ZP) ranged from 252 to 616 nm, from 0.22 to 0.73 and −50 to 30 mV, respectively. The increase of polymer proportion and the ultra-turrax homogenization led to the enlargement of particles size and PDI. However, no influence was observed on the ZP. The NP1s-Rb and NP4s-Rb, obtained through the sonicator with rifampicin (RIF) added before the CS and SA complexation, were selected due to the most promising characteristics of diameter (301 and 402 nm), PDI (0.27 and 0.26), and RIF incorporation (78 and 69%). The release profiles of RIF incorporated in both nano PECs were similar, with a sustained release of the drug for 180 min in phosphate buffer pH 7.2. The Weibull and the Korsmeyer–Peppas models better describe the RIF release from NP1s-Rb and NP4s-Rb, respectively, demonstrating that the release process was driven by different mechanism according to the particle composition. The nano PECs were lyophilized to prolong it stability and for possible nebulization. The addition of dextrose to the system allowed for resuspension after lyophilization. Therefore, with the results obtained, the incorporation of RIF in nano PECs based on CS and SA presents a promising system for the treatment of tuberculosis.Department of Drugs and Medicines Faculdade de Ciências Farmacêuticas UNESP–Univ. Estadual PaulistaDepartment of Drugs and Medicines Faculdade de Ciências Farmacêuticas UNESP–Univ. Estadual PaulistaUniversidade Estadual Paulista (UNESP)Turco, Bruna Ortolani [UNESP]Boni, Fernanda Isadora [UNESP]Gremião, Maria Palmira Daflon [UNESP]Chorilli, Marlus [UNESP]2023-03-01T20:46:29Z2023-03-01T20:46:29Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1904-1914http://dx.doi.org/10.1080/03639045.2022.2048664Drug Development and Industrial Pharmacy, v. 47, n. 12, p. 1904-1914, 2021.1520-57620363-9045http://hdl.handle.net/11449/24108710.1080/03639045.2022.20486642-s2.0-85131162569Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengDrug Development and Industrial Pharmacyinfo:eu-repo/semantics/openAccess2023-03-01T20:46:29Zoai:repositorio.unesp.br:11449/241087Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-01T20:46:29Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Nanostructured polyelectrolyte complexes based on chitosan and sodium alginate containing rifampicin for the potential treatment of tuberculosis |
title |
Nanostructured polyelectrolyte complexes based on chitosan and sodium alginate containing rifampicin for the potential treatment of tuberculosis |
spellingShingle |
Nanostructured polyelectrolyte complexes based on chitosan and sodium alginate containing rifampicin for the potential treatment of tuberculosis Turco, Bruna Ortolani [UNESP] manufacturing parameters Mycobacterium tuberculosis polyelectrolyte complexes rifampicin Tuberculosis |
title_short |
Nanostructured polyelectrolyte complexes based on chitosan and sodium alginate containing rifampicin for the potential treatment of tuberculosis |
title_full |
Nanostructured polyelectrolyte complexes based on chitosan and sodium alginate containing rifampicin for the potential treatment of tuberculosis |
title_fullStr |
Nanostructured polyelectrolyte complexes based on chitosan and sodium alginate containing rifampicin for the potential treatment of tuberculosis |
title_full_unstemmed |
Nanostructured polyelectrolyte complexes based on chitosan and sodium alginate containing rifampicin for the potential treatment of tuberculosis |
title_sort |
Nanostructured polyelectrolyte complexes based on chitosan and sodium alginate containing rifampicin for the potential treatment of tuberculosis |
author |
Turco, Bruna Ortolani [UNESP] |
author_facet |
Turco, Bruna Ortolani [UNESP] Boni, Fernanda Isadora [UNESP] Gremião, Maria Palmira Daflon [UNESP] Chorilli, Marlus [UNESP] |
author_role |
author |
author2 |
Boni, Fernanda Isadora [UNESP] Gremião, Maria Palmira Daflon [UNESP] Chorilli, Marlus [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Turco, Bruna Ortolani [UNESP] Boni, Fernanda Isadora [UNESP] Gremião, Maria Palmira Daflon [UNESP] Chorilli, Marlus [UNESP] |
dc.subject.por.fl_str_mv |
manufacturing parameters Mycobacterium tuberculosis polyelectrolyte complexes rifampicin Tuberculosis |
topic |
manufacturing parameters Mycobacterium tuberculosis polyelectrolyte complexes rifampicin Tuberculosis |
description |
Nanostructured polyelectrolyte complexes (nano PECs) were obtained by polyelectrolyte complexation technique from chitosan (CS) and sodium alginate (SA). Different polymer proportions were tested, as well as the addition order and homogenization type, to assess the influence on the nano PECs characteristics. The spherical shape and nanometric scale of the systems were observed by scanning electron microscopy (SEM). Nano PECs size, PDI, and zeta potential (ZP) ranged from 252 to 616 nm, from 0.22 to 0.73 and −50 to 30 mV, respectively. The increase of polymer proportion and the ultra-turrax homogenization led to the enlargement of particles size and PDI. However, no influence was observed on the ZP. The NP1s-Rb and NP4s-Rb, obtained through the sonicator with rifampicin (RIF) added before the CS and SA complexation, were selected due to the most promising characteristics of diameter (301 and 402 nm), PDI (0.27 and 0.26), and RIF incorporation (78 and 69%). The release profiles of RIF incorporated in both nano PECs were similar, with a sustained release of the drug for 180 min in phosphate buffer pH 7.2. The Weibull and the Korsmeyer–Peppas models better describe the RIF release from NP1s-Rb and NP4s-Rb, respectively, demonstrating that the release process was driven by different mechanism according to the particle composition. The nano PECs were lyophilized to prolong it stability and for possible nebulization. The addition of dextrose to the system allowed for resuspension after lyophilization. Therefore, with the results obtained, the incorporation of RIF in nano PECs based on CS and SA presents a promising system for the treatment of tuberculosis. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2023-03-01T20:46:29Z 2023-03-01T20:46:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/03639045.2022.2048664 Drug Development and Industrial Pharmacy, v. 47, n. 12, p. 1904-1914, 2021. 1520-5762 0363-9045 http://hdl.handle.net/11449/241087 10.1080/03639045.2022.2048664 2-s2.0-85131162569 |
url |
http://dx.doi.org/10.1080/03639045.2022.2048664 http://hdl.handle.net/11449/241087 |
identifier_str_mv |
Drug Development and Industrial Pharmacy, v. 47, n. 12, p. 1904-1914, 2021. 1520-5762 0363-9045 10.1080/03639045.2022.2048664 2-s2.0-85131162569 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Drug Development and Industrial Pharmacy |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1904-1914 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799965534737924096 |