Pigment epithelial-derived factor in human fetal membranes
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1080/14767058.2017.1335707 http://hdl.handle.net/11449/174790 |
Resumo: | Objective: Our main objective was to document, pigment epithelial-derived factor (PEDF), a secreted serine protease inhibitor with anti-angiogenic, anti-inflammatory, and anti-oxidant properties, expression in human fetal membranes from preterm prelabor rupture of the membranes (pPROM) and in in vitro cultures stimulated with cigarette smoke extract (CSE) or lipopolysaccharides (LPS), two major risk factors for pPROM (behavioral and bacterial, respectively). Method: We documented PEDF mRNA expression in clinical samples of fetal membranes from patients with pPROM using quantitative RT-PCR. Also, mRNA and protein levels were documented in fetal membranes (from normal term cesarean sections [not in labor]) in an organ explant system stimulated with CSE or lipopolysaccharide (LPS). Immunohistochemistry (IHC) was used to localize PEDF in fetal membranes. Results: We report no changes in PEDF mRNA expression in pPROM compared to term births (p =.59) or after treatment with CSE or LPS. However, by adding sulforaphane the PEDF mRNA expression increased significantly p <.000032. PEDF was localized to both amnion and chorion layers, but no difference was seen in staining intensities after CSE or LPS treatment compared to control. Conclusions: PEDF, a product of fetal membrane cells, is unaltered in pPROM or after exposure to risk factors of pPROM. The antioxidant stimulating substance sulforaphane contribute to an increase in PEDF mRNA in fetal membranes. |
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Pigment epithelial-derived factor in human fetal membranesbacterial infectionfetal membranepPROMSerpinf1smokingObjective: Our main objective was to document, pigment epithelial-derived factor (PEDF), a secreted serine protease inhibitor with anti-angiogenic, anti-inflammatory, and anti-oxidant properties, expression in human fetal membranes from preterm prelabor rupture of the membranes (pPROM) and in in vitro cultures stimulated with cigarette smoke extract (CSE) or lipopolysaccharides (LPS), two major risk factors for pPROM (behavioral and bacterial, respectively). Method: We documented PEDF mRNA expression in clinical samples of fetal membranes from patients with pPROM using quantitative RT-PCR. Also, mRNA and protein levels were documented in fetal membranes (from normal term cesarean sections [not in labor]) in an organ explant system stimulated with CSE or lipopolysaccharide (LPS). Immunohistochemistry (IHC) was used to localize PEDF in fetal membranes. Results: We report no changes in PEDF mRNA expression in pPROM compared to term births (p =.59) or after treatment with CSE or LPS. However, by adding sulforaphane the PEDF mRNA expression increased significantly p <.000032. PEDF was localized to both amnion and chorion layers, but no difference was seen in staining intensities after CSE or LPS treatment compared to control. Conclusions: PEDF, a product of fetal membrane cells, is unaltered in pPROM or after exposure to risk factors of pPROM. The antioxidant stimulating substance sulforaphane contribute to an increase in PEDF mRNA in fetal membranes.University of Texas Medical BranchUniversity of Texas Medical Branch at GalvestonDivision of Maternal–Fetal Medicine and Perinatal Research Department of Obstetrics and Gynecology The University of Texas Medical Branch at GalvestonUniversity of Gothenburg Department of Obstetrics and Gynecology Sahlgrenska University Hospital/OstraDepartment of Pathology Botucatu Medical School UNESP–Univ. Estadual PaulistaDepartment of Obstetrics and Gynecology Sahlgrenska AcademyDepartment of Genetics and Bioinformatics Area of Health Data and Digitalisation Norwegian Institute of Public HealthDepartment of Pathology Botucatu Medical School UNESP–Univ. Estadual PaulistaThe University of Texas Medical Branch at GalvestonSahlgrenska University Hospital/OstraUniversidade Estadual Paulista (Unesp)Sahlgrenska AcademyNorwegian Institute of Public HealthStalberg, CeciliaNoda, Nathalia [UNESP]Polettini, Jossimara [UNESP]Jacobson, BoMenon, Ramkumar2018-12-11T17:12:53Z2018-12-11T17:12:53Z2018-08-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2058-2065http://dx.doi.org/10.1080/14767058.2017.1335707Journal of Maternal-Fetal and Neonatal Medicine, v. 31, n. 15, p. 2058-2065, 2018.1476-49541476-7058http://hdl.handle.net/11449/17479010.1080/14767058.2017.13357072-s2.0-85021152035Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Maternal-Fetal and Neonatal Medicine0,7140,714info:eu-repo/semantics/openAccess2024-09-03T13:18:33Zoai:repositorio.unesp.br:11449/174790Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:18:33Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Pigment epithelial-derived factor in human fetal membranes |
title |
Pigment epithelial-derived factor in human fetal membranes |
spellingShingle |
Pigment epithelial-derived factor in human fetal membranes Stalberg, Cecilia bacterial infection fetal membrane pPROM Serpinf1 smoking |
title_short |
Pigment epithelial-derived factor in human fetal membranes |
title_full |
Pigment epithelial-derived factor in human fetal membranes |
title_fullStr |
Pigment epithelial-derived factor in human fetal membranes |
title_full_unstemmed |
Pigment epithelial-derived factor in human fetal membranes |
title_sort |
Pigment epithelial-derived factor in human fetal membranes |
author |
Stalberg, Cecilia |
author_facet |
Stalberg, Cecilia Noda, Nathalia [UNESP] Polettini, Jossimara [UNESP] Jacobson, Bo Menon, Ramkumar |
author_role |
author |
author2 |
Noda, Nathalia [UNESP] Polettini, Jossimara [UNESP] Jacobson, Bo Menon, Ramkumar |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
The University of Texas Medical Branch at Galveston Sahlgrenska University Hospital/Ostra Universidade Estadual Paulista (Unesp) Sahlgrenska Academy Norwegian Institute of Public Health |
dc.contributor.author.fl_str_mv |
Stalberg, Cecilia Noda, Nathalia [UNESP] Polettini, Jossimara [UNESP] Jacobson, Bo Menon, Ramkumar |
dc.subject.por.fl_str_mv |
bacterial infection fetal membrane pPROM Serpinf1 smoking |
topic |
bacterial infection fetal membrane pPROM Serpinf1 smoking |
description |
Objective: Our main objective was to document, pigment epithelial-derived factor (PEDF), a secreted serine protease inhibitor with anti-angiogenic, anti-inflammatory, and anti-oxidant properties, expression in human fetal membranes from preterm prelabor rupture of the membranes (pPROM) and in in vitro cultures stimulated with cigarette smoke extract (CSE) or lipopolysaccharides (LPS), two major risk factors for pPROM (behavioral and bacterial, respectively). Method: We documented PEDF mRNA expression in clinical samples of fetal membranes from patients with pPROM using quantitative RT-PCR. Also, mRNA and protein levels were documented in fetal membranes (from normal term cesarean sections [not in labor]) in an organ explant system stimulated with CSE or lipopolysaccharide (LPS). Immunohistochemistry (IHC) was used to localize PEDF in fetal membranes. Results: We report no changes in PEDF mRNA expression in pPROM compared to term births (p =.59) or after treatment with CSE or LPS. However, by adding sulforaphane the PEDF mRNA expression increased significantly p <.000032. PEDF was localized to both amnion and chorion layers, but no difference was seen in staining intensities after CSE or LPS treatment compared to control. Conclusions: PEDF, a product of fetal membrane cells, is unaltered in pPROM or after exposure to risk factors of pPROM. The antioxidant stimulating substance sulforaphane contribute to an increase in PEDF mRNA in fetal membranes. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:12:53Z 2018-12-11T17:12:53Z 2018-08-03 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1080/14767058.2017.1335707 Journal of Maternal-Fetal and Neonatal Medicine, v. 31, n. 15, p. 2058-2065, 2018. 1476-4954 1476-7058 http://hdl.handle.net/11449/174790 10.1080/14767058.2017.1335707 2-s2.0-85021152035 |
url |
http://dx.doi.org/10.1080/14767058.2017.1335707 http://hdl.handle.net/11449/174790 |
identifier_str_mv |
Journal of Maternal-Fetal and Neonatal Medicine, v. 31, n. 15, p. 2058-2065, 2018. 1476-4954 1476-7058 10.1080/14767058.2017.1335707 2-s2.0-85021152035 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Journal of Maternal-Fetal and Neonatal Medicine 0,714 0,714 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
2058-2065 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021417520988160 |