Effect of physical training on cytokine expression in CD4+ T lymphocytes in subjects with stable COPD
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1177/17534666221091179 http://hdl.handle.net/11449/241935 |
Resumo: | Introduction: Although evidence suggests that physical exercise reduces systemic inflammation, at the plasma level, there are still contradictions in chronic obstructive pulmonary disease (COPD). In this sense, analysis of intracellular cytokines could clear off the effect of physical exercise on the inflammatory profile of these subjects. Aim: The aim was to evaluate the effect of physical training on cytokine expression in CD4+ T lymphocytes from subjects with COPD. Methods: This is a randomized controlled trial. Subjects with stable COPD were grouped into two groups, exercise and control. In total, 23 subjects with stable COPD were evaluated, of which 15 underwent aerobic strength training [physical exercise group (PEG)] and 8 underwent breathing exercises [respiratory physiotherapy group (RPG)]. Intracellular cytokines [interleukin (IL)-8, IL-13, IL-17, IL-6, IL-2, IL-10, and tumor necrosis factor alpha (TNF-α)] from CD4+ T lymphocytes were analyzed from peripheral blood through flow cytometry, before and after 8 weeks of intervention. Results: The PEG and RPG groups had a mean age of 68 ± 5.96 and 72.25 ± 6.86 years and predicted forced expiratory volume in the first second (FEV1) of 58.6 ± 15.99% and 39.75 ± 10.39%, respectively. It was possible to detect a significant reduction in IL-8 (p = 0.0125) and an increase in IL-13 (p = 0.0014) and an increase in TNF-α (p < 0.001) in both groups. Conclusion: Eight weeks of physical training, both peripheral and respiratory, were able to reduce concentrations of IL-8 and to increase IL-13, and TNF-α in CD4+ T lymphocytes in subjects with stable COPD. The findings reinforce the benefits of interventions in subjects with COPD, revealing data not previously investigated. |
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Effect of physical training on cytokine expression in CD4+ T lymphocytes in subjects with stable COPDchronic obstructive pulmonary diseaseflow cytometryinflammationphysical exerciseIntroduction: Although evidence suggests that physical exercise reduces systemic inflammation, at the plasma level, there are still contradictions in chronic obstructive pulmonary disease (COPD). In this sense, analysis of intracellular cytokines could clear off the effect of physical exercise on the inflammatory profile of these subjects. Aim: The aim was to evaluate the effect of physical training on cytokine expression in CD4+ T lymphocytes from subjects with COPD. Methods: This is a randomized controlled trial. Subjects with stable COPD were grouped into two groups, exercise and control. In total, 23 subjects with stable COPD were evaluated, of which 15 underwent aerobic strength training [physical exercise group (PEG)] and 8 underwent breathing exercises [respiratory physiotherapy group (RPG)]. Intracellular cytokines [interleukin (IL)-8, IL-13, IL-17, IL-6, IL-2, IL-10, and tumor necrosis factor alpha (TNF-α)] from CD4+ T lymphocytes were analyzed from peripheral blood through flow cytometry, before and after 8 weeks of intervention. Results: The PEG and RPG groups had a mean age of 68 ± 5.96 and 72.25 ± 6.86 years and predicted forced expiratory volume in the first second (FEV1) of 58.6 ± 15.99% and 39.75 ± 10.39%, respectively. It was possible to detect a significant reduction in IL-8 (p = 0.0125) and an increase in IL-13 (p = 0.0014) and an increase in TNF-α (p < 0.001) in both groups. Conclusion: Eight weeks of physical training, both peripheral and respiratory, were able to reduce concentrations of IL-8 and to increase IL-13, and TNF-α in CD4+ T lymphocytes in subjects with stable COPD. The findings reinforce the benefits of interventions in subjects with COPD, revealing data not previously investigated.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Physiotherapy Postgraduate Program in Physiotherapy Faculty of Science and Technology São Paulo State University (UNESP), Rua Roberto Simonsen, 305, São PauloDepartment of Physiology and Pharmacology Laboratory of Exercise Sciences Fluminense Federal UniversityUniversidade Estadual Paulista Julio de Mesquita Filho PhysiotherapyUniversidade Estadual Paulista Julio de Mesquita FilhoBotucatu Medical School Postgraduate Program in Research Development: Medical Biotechnology Blood Center Flow Cytometry Laboratory São Paulo State University (UNESP)Department of Physiotherapy Postgraduate Program in Physiotherapy Faculty of Science and Technology São Paulo State University (UNESP), Rua Roberto Simonsen, 305, São PauloUniversidade Estadual Paulista Julio de Mesquita Filho PhysiotherapyUniversidade Estadual Paulista Julio de Mesquita FilhoBotucatu Medical School Postgraduate Program in Research Development: Medical Biotechnology Blood Center Flow Cytometry Laboratory São Paulo State University (UNESP)CAPES: 001FAPESP: 2017/10145-7FAPESP: 2018/04870-3Universidade Estadual Paulista (UNESP)Fluminense Federal UniversityUzeloto, Juliana S. [UNESP]de Toledo-Arruda, Alessandra C.Silva, Bruna S.A. [UNESP]Braz, Aline M.M. [UNESP]de Lima, Fabiano F. [UNESP]Grigoletto, Isis [UNESP]Ramos, Dionei [UNESP]Golim, Marjorie A. [UNESP]Ramos, Ercy M.C. [UNESP]2023-03-02T04:42:42Z2023-03-02T04:42:42Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1177/17534666221091179Therapeutic Advances in Respiratory Disease, v. 16.1753-46661753-4658http://hdl.handle.net/11449/24193510.1177/175346662210911792-s2.0-85131851193Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengTherapeutic Advances in Respiratory Diseaseinfo:eu-repo/semantics/openAccess2024-06-18T18:44:01Zoai:repositorio.unesp.br:11449/241935Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:14:26.412577Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effect of physical training on cytokine expression in CD4+ T lymphocytes in subjects with stable COPD |
title |
Effect of physical training on cytokine expression in CD4+ T lymphocytes in subjects with stable COPD |
spellingShingle |
Effect of physical training on cytokine expression in CD4+ T lymphocytes in subjects with stable COPD Uzeloto, Juliana S. [UNESP] chronic obstructive pulmonary disease flow cytometry inflammation physical exercise |
title_short |
Effect of physical training on cytokine expression in CD4+ T lymphocytes in subjects with stable COPD |
title_full |
Effect of physical training on cytokine expression in CD4+ T lymphocytes in subjects with stable COPD |
title_fullStr |
Effect of physical training on cytokine expression in CD4+ T lymphocytes in subjects with stable COPD |
title_full_unstemmed |
Effect of physical training on cytokine expression in CD4+ T lymphocytes in subjects with stable COPD |
title_sort |
Effect of physical training on cytokine expression in CD4+ T lymphocytes in subjects with stable COPD |
author |
Uzeloto, Juliana S. [UNESP] |
author_facet |
Uzeloto, Juliana S. [UNESP] de Toledo-Arruda, Alessandra C. Silva, Bruna S.A. [UNESP] Braz, Aline M.M. [UNESP] de Lima, Fabiano F. [UNESP] Grigoletto, Isis [UNESP] Ramos, Dionei [UNESP] Golim, Marjorie A. [UNESP] Ramos, Ercy M.C. [UNESP] |
author_role |
author |
author2 |
de Toledo-Arruda, Alessandra C. Silva, Bruna S.A. [UNESP] Braz, Aline M.M. [UNESP] de Lima, Fabiano F. [UNESP] Grigoletto, Isis [UNESP] Ramos, Dionei [UNESP] Golim, Marjorie A. [UNESP] Ramos, Ercy M.C. [UNESP] |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) Fluminense Federal University |
dc.contributor.author.fl_str_mv |
Uzeloto, Juliana S. [UNESP] de Toledo-Arruda, Alessandra C. Silva, Bruna S.A. [UNESP] Braz, Aline M.M. [UNESP] de Lima, Fabiano F. [UNESP] Grigoletto, Isis [UNESP] Ramos, Dionei [UNESP] Golim, Marjorie A. [UNESP] Ramos, Ercy M.C. [UNESP] |
dc.subject.por.fl_str_mv |
chronic obstructive pulmonary disease flow cytometry inflammation physical exercise |
topic |
chronic obstructive pulmonary disease flow cytometry inflammation physical exercise |
description |
Introduction: Although evidence suggests that physical exercise reduces systemic inflammation, at the plasma level, there are still contradictions in chronic obstructive pulmonary disease (COPD). In this sense, analysis of intracellular cytokines could clear off the effect of physical exercise on the inflammatory profile of these subjects. Aim: The aim was to evaluate the effect of physical training on cytokine expression in CD4+ T lymphocytes from subjects with COPD. Methods: This is a randomized controlled trial. Subjects with stable COPD were grouped into two groups, exercise and control. In total, 23 subjects with stable COPD were evaluated, of which 15 underwent aerobic strength training [physical exercise group (PEG)] and 8 underwent breathing exercises [respiratory physiotherapy group (RPG)]. Intracellular cytokines [interleukin (IL)-8, IL-13, IL-17, IL-6, IL-2, IL-10, and tumor necrosis factor alpha (TNF-α)] from CD4+ T lymphocytes were analyzed from peripheral blood through flow cytometry, before and after 8 weeks of intervention. Results: The PEG and RPG groups had a mean age of 68 ± 5.96 and 72.25 ± 6.86 years and predicted forced expiratory volume in the first second (FEV1) of 58.6 ± 15.99% and 39.75 ± 10.39%, respectively. It was possible to detect a significant reduction in IL-8 (p = 0.0125) and an increase in IL-13 (p = 0.0014) and an increase in TNF-α (p < 0.001) in both groups. Conclusion: Eight weeks of physical training, both peripheral and respiratory, were able to reduce concentrations of IL-8 and to increase IL-13, and TNF-α in CD4+ T lymphocytes in subjects with stable COPD. The findings reinforce the benefits of interventions in subjects with COPD, revealing data not previously investigated. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-02T04:42:42Z 2023-03-02T04:42:42Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1177/17534666221091179 Therapeutic Advances in Respiratory Disease, v. 16. 1753-4666 1753-4658 http://hdl.handle.net/11449/241935 10.1177/17534666221091179 2-s2.0-85131851193 |
url |
http://dx.doi.org/10.1177/17534666221091179 http://hdl.handle.net/11449/241935 |
identifier_str_mv |
Therapeutic Advances in Respiratory Disease, v. 16. 1753-4666 1753-4658 10.1177/17534666221091179 2-s2.0-85131851193 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Therapeutic Advances in Respiratory Disease |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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_version_ |
1808128910770044928 |