Perfil bioquímico dos soros lácteo e sanguíneo de cabras com mastite de ocorrência natural
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://hdl.handle.net/11449/143029 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/14-07-2016/000864826.pdf |
Resumo: | The study aimed to evaluate the changes in milk from goats with mastitis by using the strip cup test, California Mastitis Test (CMT), somatic cell count (SCC) and microbiological profile as well as to determine the biochemical profile, especially acute phase proteins, in blood serum and whey from goats with naturally occurring clinical or subclinical mastitis. Three experimental groups were formed by 30 mammary glands from healthy goats with milk samples negative to strip cup test, CMT and microbiological examination (control group - G1); 30 mammary glands with milk samples negative to strip cup test but with moderate (2+) or intense (3+) reaction in CMT and microbiological examination (group with subclinical mastitis - G2), and 12 mammary glands with positive results to strip cup test and microbiological examination (group with clinical mastitis - G3). After asepsis of the teats milk samples were collected for SCC, microbiological culture, and to determine the biochemical profile in whey, including proteinogram by fractionation in poliacrylamide gel electrophoresis (SDS-PAGE). At the same time, blood samples were obtained to determine the serum biochemical profile. Automatic and microscopic methods were higher in milk samples from goats with subclinical mastits (G2: 4,500.000 cells/mL e 20,830,000 cells/mL, respectively) e with clinical mastitis (G3: 7,500,000 células/mL e 39,100,000 cells/mL, respectively). Staphylococcus aureus, Coagulase-negative Staphylococcus (CNS), Coagulase-positive Staphylococcus (CPS), Corynebaterium spp. and Streptococcus spp. were the microorganisms isolated in milk samples from goats of G2 and G3. Whey samples from goats of G2 and G3 showed higher activity of alkaline phosphatase (G2: 122 U/L and G3: 207 U/L) and gamma-glutamyltransferase (G2: 483 U/L and G3: 571 U / L), and higher concentrations of albumin (G2: 0.18 g/dL and G3: 0.29 g/dL), total protein (G2: 1.50 g/dL and G3: 1.67 g/dL), chlorides ... |
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Perfil bioquímico dos soros lácteo e sanguíneo de cabras com mastite de ocorrência naturalCabraGlandulas mamariasInflamaçãoMastiteProteínasMastitisThe study aimed to evaluate the changes in milk from goats with mastitis by using the strip cup test, California Mastitis Test (CMT), somatic cell count (SCC) and microbiological profile as well as to determine the biochemical profile, especially acute phase proteins, in blood serum and whey from goats with naturally occurring clinical or subclinical mastitis. Three experimental groups were formed by 30 mammary glands from healthy goats with milk samples negative to strip cup test, CMT and microbiological examination (control group - G1); 30 mammary glands with milk samples negative to strip cup test but with moderate (2+) or intense (3+) reaction in CMT and microbiological examination (group with subclinical mastitis - G2), and 12 mammary glands with positive results to strip cup test and microbiological examination (group with clinical mastitis - G3). After asepsis of the teats milk samples were collected for SCC, microbiological culture, and to determine the biochemical profile in whey, including proteinogram by fractionation in poliacrylamide gel electrophoresis (SDS-PAGE). At the same time, blood samples were obtained to determine the serum biochemical profile. Automatic and microscopic methods were higher in milk samples from goats with subclinical mastits (G2: 4,500.000 cells/mL e 20,830,000 cells/mL, respectively) e with clinical mastitis (G3: 7,500,000 células/mL e 39,100,000 cells/mL, respectively). Staphylococcus aureus, Coagulase-negative Staphylococcus (CNS), Coagulase-positive Staphylococcus (CPS), Corynebaterium spp. and Streptococcus spp. were the microorganisms isolated in milk samples from goats of G2 and G3. Whey samples from goats of G2 and G3 showed higher activity of alkaline phosphatase (G2: 122 U/L and G3: 207 U/L) and gamma-glutamyltransferase (G2: 483 U/L and G3: 571 U / L), and higher concentrations of albumin (G2: 0.18 g/dL and G3: 0.29 g/dL), total protein (G2: 1.50 g/dL and G3: 1.67 g/dL), chlorides ...O estudo teve como objetivo avaliar as alterações da secreção láctea de cabras com mastite mediante o uso do teste da caneca de fundo escuro, California Mastitis Test (CMT), contagem de células somáticas (CCS) e perfil microbiológico, bem como determinar o perfil bioquímico, em especial de proteínas de fase aguda, do soro sanguíneo e soro lácteo de cabras com mastite clínica ou subclínica, de ocorrência natural. Foram constituídos três grupos experimentais compostos de 30 metades mamárias de cabras sadias, com secreção láctea negativa na prova da caneca de fundo escuro, CMT e no exame microbiológico (grupo controle - G1), 30 metades mamárias com secreção láctea negativa ao teste da caneca de fundo escuro, reação moderada (2+) ou intensa (3+) no CMT e positividade no exame microbiológico (Grupo mastite subclínica - G2) e 12 metades mamárias com secreção láctea positiva na prova da caneca de fundo escuro e no exame microbiológico (grupo mastite clínica - G3). Após antissepsia dos tetos foram colhidas amostras de leite para CCS, cultura microbiológica e determinação do perfil bioquímico do soro lácteo, inclusive o proteinograma mediante fracionamento em gel de poliacrilamida (SDS-PAGE), e amostras de sangue venoso para determinação do perfil bioquímico sérico. A contagem automática de células somáticas (CCS) e por contagem microscopia direta foi maior nas amostras de secreção láctea das cabras com mastite subclínica (G2: 4.500.000 células/mL e 20.830.000 células/mL, respectivamente) e com mastite clínica (G3: 7.500.000 células/mL e 39.100.000 células/mL, respectivamente). Staphylococcus aureus, Staphylococcus coagulase negativa (SCN), Staphylococcus coagulase positiva (SCP), Corynebacterium spp. e Streptococcus spp. foram os microrganismos isolados nas amostras de leite das cabras do G2 e G3. As amostras de soro lácteo das cabras do G2 e G3 apresentaram maiores atividades das enzimas...Universidade Estadual Paulista (Unesp)Fagliari, José Jurandir [UNESP]Vidal, Ana Maria Centola [UNESP]Universidade Estadual Paulista (Unesp)Sanchez, Diana Consuelo Cifuentes [UNESP]2016-08-12T18:48:53Z2016-08-12T18:48:53Z2015-12-08info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisxii, 49 p. : il.application/pdfSANCHEZ, Diana Consuelo Cifuentes. Perfil bioquímico dos soros lácteo e sanguíneo de cabras com mastite de ocorrência natural. 2015. xii, 49 p. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2015.http://hdl.handle.net/11449/143029000864826http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/14-07-2016/000864826.pdf33004102072P9Alephreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporinfo:eu-repo/semantics/openAccess2023-09-30T06:03:04Zoai:repositorio.unesp.br:11449/143029Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-09-30T06:03:04Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Perfil bioquímico dos soros lácteo e sanguíneo de cabras com mastite de ocorrência natural |
title |
Perfil bioquímico dos soros lácteo e sanguíneo de cabras com mastite de ocorrência natural |
spellingShingle |
Perfil bioquímico dos soros lácteo e sanguíneo de cabras com mastite de ocorrência natural Sanchez, Diana Consuelo Cifuentes [UNESP] Cabra Glandulas mamarias Inflamação Mastite Proteínas Mastitis |
title_short |
Perfil bioquímico dos soros lácteo e sanguíneo de cabras com mastite de ocorrência natural |
title_full |
Perfil bioquímico dos soros lácteo e sanguíneo de cabras com mastite de ocorrência natural |
title_fullStr |
Perfil bioquímico dos soros lácteo e sanguíneo de cabras com mastite de ocorrência natural |
title_full_unstemmed |
Perfil bioquímico dos soros lácteo e sanguíneo de cabras com mastite de ocorrência natural |
title_sort |
Perfil bioquímico dos soros lácteo e sanguíneo de cabras com mastite de ocorrência natural |
author |
Sanchez, Diana Consuelo Cifuentes [UNESP] |
author_facet |
Sanchez, Diana Consuelo Cifuentes [UNESP] |
author_role |
author |
dc.contributor.none.fl_str_mv |
Fagliari, José Jurandir [UNESP] Vidal, Ana Maria Centola [UNESP] Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Sanchez, Diana Consuelo Cifuentes [UNESP] |
dc.subject.por.fl_str_mv |
Cabra Glandulas mamarias Inflamação Mastite Proteínas Mastitis |
topic |
Cabra Glandulas mamarias Inflamação Mastite Proteínas Mastitis |
description |
The study aimed to evaluate the changes in milk from goats with mastitis by using the strip cup test, California Mastitis Test (CMT), somatic cell count (SCC) and microbiological profile as well as to determine the biochemical profile, especially acute phase proteins, in blood serum and whey from goats with naturally occurring clinical or subclinical mastitis. Three experimental groups were formed by 30 mammary glands from healthy goats with milk samples negative to strip cup test, CMT and microbiological examination (control group - G1); 30 mammary glands with milk samples negative to strip cup test but with moderate (2+) or intense (3+) reaction in CMT and microbiological examination (group with subclinical mastitis - G2), and 12 mammary glands with positive results to strip cup test and microbiological examination (group with clinical mastitis - G3). After asepsis of the teats milk samples were collected for SCC, microbiological culture, and to determine the biochemical profile in whey, including proteinogram by fractionation in poliacrylamide gel electrophoresis (SDS-PAGE). At the same time, blood samples were obtained to determine the serum biochemical profile. Automatic and microscopic methods were higher in milk samples from goats with subclinical mastits (G2: 4,500.000 cells/mL e 20,830,000 cells/mL, respectively) e with clinical mastitis (G3: 7,500,000 células/mL e 39,100,000 cells/mL, respectively). Staphylococcus aureus, Coagulase-negative Staphylococcus (CNS), Coagulase-positive Staphylococcus (CPS), Corynebaterium spp. and Streptococcus spp. were the microorganisms isolated in milk samples from goats of G2 and G3. Whey samples from goats of G2 and G3 showed higher activity of alkaline phosphatase (G2: 122 U/L and G3: 207 U/L) and gamma-glutamyltransferase (G2: 483 U/L and G3: 571 U / L), and higher concentrations of albumin (G2: 0.18 g/dL and G3: 0.29 g/dL), total protein (G2: 1.50 g/dL and G3: 1.67 g/dL), chlorides ... |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-08 2016-08-12T18:48:53Z 2016-08-12T18:48:53Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
format |
doctoralThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
SANCHEZ, Diana Consuelo Cifuentes. Perfil bioquímico dos soros lácteo e sanguíneo de cabras com mastite de ocorrência natural. 2015. xii, 49 p. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2015. http://hdl.handle.net/11449/143029 000864826 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/14-07-2016/000864826.pdf 33004102072P9 |
identifier_str_mv |
SANCHEZ, Diana Consuelo Cifuentes. Perfil bioquímico dos soros lácteo e sanguíneo de cabras com mastite de ocorrência natural. 2015. xii, 49 p. Tese (doutorado) - Universidade Estadual Paulista Júlio de Mesquita Filho, Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, 2015. 000864826 33004102072P9 |
url |
http://hdl.handle.net/11449/143029 http://www.athena.biblioteca.unesp.br/exlibris/bd/cathedra/14-07-2016/000864826.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
xii, 49 p. : il. application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
publisher.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.source.none.fl_str_mv |
Aleph reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1792961420039553024 |