Proteome of the phytopathogen Xanthomonas citri subsp citri: a global expression profile
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/1477-5956-8-55 http://hdl.handle.net/11449/4091 |
Resumo: | Background: Citrus canker is a disease caused by Xantomonas citri subsp. citri (Xac), and has emerged as one of the major threats to the worldwide citrus crop because it affects all commercial citrus varieties, decreases the production and quality of the fruits and can spread rapidly in citrus growing areas. In this work, the first proteome of Xac was analyzed using two methodologies, two-dimensional liquid chromatography (2D LC) and tandem mass spectrometry (MS/MS).Results: In order to gain insight into the metabolism of Xac, cells were grown on two different media (NB - Nutrient Broth and TSE - Tryptone Sucrose broth enriched with glutamic acid), and proteins were proteolyzed with trypsin and examined by 2D LC-MS/MS. Approximately 39% of all predicted proteins by annotation of Xac were identified with their component peptides unambiguously assigned to tandem mass spectra. The proteins, about 1,100, were distributed in all annotated functional categories.Conclusions: This is the first proteomic reference map for the most aggressive strain of Xanthomonas pathogen of all orange varieties. The compilation of metabolic pathways involved with bacterial growth showed that Xac expresses a complete central and intermediary metabolism, replication, transcription and translation machineries and regulation factors, distinct membrane transporters (ABC, MFS and pumps) and receptors (MCP, TonB dependent and metabolites acquisition), two-component systems (sensor and regulatory components) and response regulators. These data corroborate the growth curve in vitro and are the first reports indicating that many of these genome annotated genes are translated into operative in Xac. This proteomic analysis also provided information regarding the influence of culture medium on growth and protein expression of Xac. |
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Proteome of the phytopathogen Xanthomonas citri subsp citri: a global expression profileBackground: Citrus canker is a disease caused by Xantomonas citri subsp. citri (Xac), and has emerged as one of the major threats to the worldwide citrus crop because it affects all commercial citrus varieties, decreases the production and quality of the fruits and can spread rapidly in citrus growing areas. In this work, the first proteome of Xac was analyzed using two methodologies, two-dimensional liquid chromatography (2D LC) and tandem mass spectrometry (MS/MS).Results: In order to gain insight into the metabolism of Xac, cells were grown on two different media (NB - Nutrient Broth and TSE - Tryptone Sucrose broth enriched with glutamic acid), and proteins were proteolyzed with trypsin and examined by 2D LC-MS/MS. Approximately 39% of all predicted proteins by annotation of Xac were identified with their component peptides unambiguously assigned to tandem mass spectra. The proteins, about 1,100, were distributed in all annotated functional categories.Conclusions: This is the first proteomic reference map for the most aggressive strain of Xanthomonas pathogen of all orange varieties. The compilation of metabolic pathways involved with bacterial growth showed that Xac expresses a complete central and intermediary metabolism, replication, transcription and translation machineries and regulation factors, distinct membrane transporters (ABC, MFS and pumps) and receptors (MCP, TonB dependent and metabolites acquisition), two-component systems (sensor and regulatory components) and response regulators. These data corroborate the growth curve in vitro and are the first reports indicating that many of these genome annotated genes are translated into operative in Xac. This proteomic analysis also provided information regarding the influence of culture medium on growth and protein expression of Xac.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)LNLS, Campinas, SP, BrazilUniv Fed Rio de Janeiro, Inst Quim, Dept Bioquim, Rio de Janeiro, BrazilUniv Estadual Paulista UNESP, Dept Tecnol, Fac Ciencias Agr & Vet Jaboticabal, Jaboticabal, SP, BrazilUniv Fed Ouro Preto, Inst Ciencias Exatas & Biol, Dept Ciencias Biol, Ouro Preto, MG, BrazilUniv Fed Ouro Preto, Nucleo Pesquisas Ciencias Biol NUPEB, Ouro Preto, MG, BrazilUniv Fed São Paulo, Dept Ciencias Biol, Diadema, SP, BrazilUniv Fed São Paulo, Fundação Apoio, São Paulo, BrazilUniv Estadual Campinas, Inst Quim, UNICAMP, Campinas, SP, BrazilUniv Estadual Paulista UNESP, Dept Tecnol, Fac Ciencias Agr & Vet Jaboticabal, Jaboticabal, SP, BrazilFAPESP: 04/02006-7Biomed Central Ltd.LNLSUniversidade Federal do Rio de Janeiro (UFRJ)Universidade Estadual Paulista (Unesp)Universidade Federal de Ouro Preto (UFOP)Universidade Federal de São Paulo (UNIFESP)Universidade Estadual de Campinas (UNICAMP)Soares, Marcia R.Facincani, Agda P. [UNESP]Ferreira, Rafael M. [UNESP]Moreira, Leandro M.de Oliveira, Julio C. F.Ferro, Jesus A. [UNESP]Ferro, Maria I. T. [UNESP]Meneghini, RogerioGozzo, Fabio C.2014-05-20T13:17:43Z2014-05-20T13:17:43Z2010-11-09info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://dx.doi.org/10.1186/1477-5956-8-55Proteome Science. London: Biomed Central Ltd., v. 8, p. 11, 2010.1477-5956http://hdl.handle.net/11449/409110.1186/1477-5956-8-55WOS:000284908500001WOS000284908500001.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengProteome Science1.7690,792info:eu-repo/semantics/openAccess2023-10-26T06:09:03Zoai:repositorio.unesp.br:11449/4091Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-10-26T06:09:03Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Proteome of the phytopathogen Xanthomonas citri subsp citri: a global expression profile |
title |
Proteome of the phytopathogen Xanthomonas citri subsp citri: a global expression profile |
spellingShingle |
Proteome of the phytopathogen Xanthomonas citri subsp citri: a global expression profile Soares, Marcia R. |
title_short |
Proteome of the phytopathogen Xanthomonas citri subsp citri: a global expression profile |
title_full |
Proteome of the phytopathogen Xanthomonas citri subsp citri: a global expression profile |
title_fullStr |
Proteome of the phytopathogen Xanthomonas citri subsp citri: a global expression profile |
title_full_unstemmed |
Proteome of the phytopathogen Xanthomonas citri subsp citri: a global expression profile |
title_sort |
Proteome of the phytopathogen Xanthomonas citri subsp citri: a global expression profile |
author |
Soares, Marcia R. |
author_facet |
Soares, Marcia R. Facincani, Agda P. [UNESP] Ferreira, Rafael M. [UNESP] Moreira, Leandro M. de Oliveira, Julio C. F. Ferro, Jesus A. [UNESP] Ferro, Maria I. T. [UNESP] Meneghini, Rogerio Gozzo, Fabio C. |
author_role |
author |
author2 |
Facincani, Agda P. [UNESP] Ferreira, Rafael M. [UNESP] Moreira, Leandro M. de Oliveira, Julio C. F. Ferro, Jesus A. [UNESP] Ferro, Maria I. T. [UNESP] Meneghini, Rogerio Gozzo, Fabio C. |
author2_role |
author author author author author author author author |
dc.contributor.none.fl_str_mv |
LNLS Universidade Federal do Rio de Janeiro (UFRJ) Universidade Estadual Paulista (Unesp) Universidade Federal de Ouro Preto (UFOP) Universidade Federal de São Paulo (UNIFESP) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Soares, Marcia R. Facincani, Agda P. [UNESP] Ferreira, Rafael M. [UNESP] Moreira, Leandro M. de Oliveira, Julio C. F. Ferro, Jesus A. [UNESP] Ferro, Maria I. T. [UNESP] Meneghini, Rogerio Gozzo, Fabio C. |
description |
Background: Citrus canker is a disease caused by Xantomonas citri subsp. citri (Xac), and has emerged as one of the major threats to the worldwide citrus crop because it affects all commercial citrus varieties, decreases the production and quality of the fruits and can spread rapidly in citrus growing areas. In this work, the first proteome of Xac was analyzed using two methodologies, two-dimensional liquid chromatography (2D LC) and tandem mass spectrometry (MS/MS).Results: In order to gain insight into the metabolism of Xac, cells were grown on two different media (NB - Nutrient Broth and TSE - Tryptone Sucrose broth enriched with glutamic acid), and proteins were proteolyzed with trypsin and examined by 2D LC-MS/MS. Approximately 39% of all predicted proteins by annotation of Xac were identified with their component peptides unambiguously assigned to tandem mass spectra. The proteins, about 1,100, were distributed in all annotated functional categories.Conclusions: This is the first proteomic reference map for the most aggressive strain of Xanthomonas pathogen of all orange varieties. The compilation of metabolic pathways involved with bacterial growth showed that Xac expresses a complete central and intermediary metabolism, replication, transcription and translation machineries and regulation factors, distinct membrane transporters (ABC, MFS and pumps) and receptors (MCP, TonB dependent and metabolites acquisition), two-component systems (sensor and regulatory components) and response regulators. These data corroborate the growth curve in vitro and are the first reports indicating that many of these genome annotated genes are translated into operative in Xac. This proteomic analysis also provided information regarding the influence of culture medium on growth and protein expression of Xac. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-11-09 2014-05-20T13:17:43Z 2014-05-20T13:17:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/1477-5956-8-55 Proteome Science. London: Biomed Central Ltd., v. 8, p. 11, 2010. 1477-5956 http://hdl.handle.net/11449/4091 10.1186/1477-5956-8-55 WOS:000284908500001 WOS000284908500001.pdf |
url |
http://dx.doi.org/10.1186/1477-5956-8-55 http://hdl.handle.net/11449/4091 |
identifier_str_mv |
Proteome Science. London: Biomed Central Ltd., v. 8, p. 11, 2010. 1477-5956 10.1186/1477-5956-8-55 WOS:000284908500001 WOS000284908500001.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Proteome Science 1.769 0,792 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 application/pdf |
dc.publisher.none.fl_str_mv |
Biomed Central Ltd. |
publisher.none.fl_str_mv |
Biomed Central Ltd. |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1797789517726351360 |