Assessment of selenium spatial distribution using μ-XFR in cowpea (Vigna unguiculata (L.) Walp.) plants: Integration of physiological and biochemical responses
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ecoenv.2020.111216 http://hdl.handle.net/11449/205166 |
Resumo: | Low concentrations of selenium (Se) are beneficial for plant growth. Foliar Se application at high concentrations is toxic to plants due to the formation of reactive oxygen species (ROS). This study characterized Se toxicity symptoms using X-ray fluorescence (XRF) technique in response to foliar Se application in cowpea plants. Five Se concentrations (0, 10, 25, 50, 100 e 150 g ha−1) were sprayed on leaves as sodium selenate. The visual symptoms of Se toxicity in cowpea leaves were separated into two stages: I) necrotic points with an irregular distribution and internerval chlorosis at the leaf limb border (50–100 g ha−1); II) total chlorosis with the formation of dark brown necrotic lesions (150 g ha−1). Foliar Se application at 50 g ha−1 increased photosynthetic pigments and yield. Ultrastructural analyses showed that Se foliar application above 50 g ha−1 disarranged the upper epidermis of cowpea leaves. Furthermore, Se application above 100 g ha−1 significantly increased the hydrogen peroxide concentration and lipid peroxidation inducing necrotic leaf lesions. Mapping of the elements in leaves using the XRF revealed high Se intensity, specifically in leaf necrotic lesions accompanied by calcium (Ca) as a possible attenuating mechanism of plant stress. The distribution of Se intensities in the seeds was homogeneous, without specific accumulation sites. Phosphorus (P) and sulfur (S) were found primarily located in the embryonic region. Understanding the factors involved in Se accumulation and its interaction with Ca support new preventive measurement technologies to prevent Se toxicity in plants. |
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Assessment of selenium spatial distribution using μ-XFR in cowpea (Vigna unguiculata (L.) Walp.) plants: Integration of physiological and biochemical responsesAgronomic biofortificationAntioxidant metabolismScanning electron microscopySeleniumVigna unguiculataX-ray fluorescence microscopyLow concentrations of selenium (Se) are beneficial for plant growth. Foliar Se application at high concentrations is toxic to plants due to the formation of reactive oxygen species (ROS). This study characterized Se toxicity symptoms using X-ray fluorescence (XRF) technique in response to foliar Se application in cowpea plants. Five Se concentrations (0, 10, 25, 50, 100 e 150 g ha−1) were sprayed on leaves as sodium selenate. The visual symptoms of Se toxicity in cowpea leaves were separated into two stages: I) necrotic points with an irregular distribution and internerval chlorosis at the leaf limb border (50–100 g ha−1); II) total chlorosis with the formation of dark brown necrotic lesions (150 g ha−1). Foliar Se application at 50 g ha−1 increased photosynthetic pigments and yield. Ultrastructural analyses showed that Se foliar application above 50 g ha−1 disarranged the upper epidermis of cowpea leaves. Furthermore, Se application above 100 g ha−1 significantly increased the hydrogen peroxide concentration and lipid peroxidation inducing necrotic leaf lesions. Mapping of the elements in leaves using the XRF revealed high Se intensity, specifically in leaf necrotic lesions accompanied by calcium (Ca) as a possible attenuating mechanism of plant stress. The distribution of Se intensities in the seeds was homogeneous, without specific accumulation sites. Phosphorus (P) and sulfur (S) were found primarily located in the embryonic region. Understanding the factors involved in Se accumulation and its interaction with Ca support new preventive measurement technologies to prevent Se toxicity in plants.São Paulo State University (UNESP), Via de Acesso Prof. Paulo Donato Castellane S/n, Postal Code 14884-900University of São Paulo (USP), Av. Centenário, 303, São DimasSão Paulo State University (UNESP), Rua Domingos da Costa Lopes 780São Paulo State University (UNESP), Via de Acesso Prof. Paulo Donato Castellane S/n, Postal Code 14884-900São Paulo State University (UNESP), Rua Domingos da Costa Lopes 780Universidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Lanza, Maria Gabriela Dantas Bereta [UNESP]Silva, Vinícius Martins [UNESP]Montanha, Gabriel SgarbieroLavres, JoséPereira de Carvalho, Hudson WallaceReis, André Rodrigues dos [UNESP]2021-06-25T10:10:59Z2021-06-25T10:10:59Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.ecoenv.2020.111216Ecotoxicology and Environmental Safety, v. 207.1090-24140147-6513http://hdl.handle.net/11449/20516610.1016/j.ecoenv.2020.1112162-s2.0-85090322594Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEcotoxicology and Environmental Safetyinfo:eu-repo/semantics/openAccess2021-10-23T11:11:15Zoai:repositorio.unesp.br:11449/205166Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:30:09.667692Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Assessment of selenium spatial distribution using μ-XFR in cowpea (Vigna unguiculata (L.) Walp.) plants: Integration of physiological and biochemical responses |
title |
Assessment of selenium spatial distribution using μ-XFR in cowpea (Vigna unguiculata (L.) Walp.) plants: Integration of physiological and biochemical responses |
spellingShingle |
Assessment of selenium spatial distribution using μ-XFR in cowpea (Vigna unguiculata (L.) Walp.) plants: Integration of physiological and biochemical responses Lanza, Maria Gabriela Dantas Bereta [UNESP] Agronomic biofortification Antioxidant metabolism Scanning electron microscopy Selenium Vigna unguiculata X-ray fluorescence microscopy |
title_short |
Assessment of selenium spatial distribution using μ-XFR in cowpea (Vigna unguiculata (L.) Walp.) plants: Integration of physiological and biochemical responses |
title_full |
Assessment of selenium spatial distribution using μ-XFR in cowpea (Vigna unguiculata (L.) Walp.) plants: Integration of physiological and biochemical responses |
title_fullStr |
Assessment of selenium spatial distribution using μ-XFR in cowpea (Vigna unguiculata (L.) Walp.) plants: Integration of physiological and biochemical responses |
title_full_unstemmed |
Assessment of selenium spatial distribution using μ-XFR in cowpea (Vigna unguiculata (L.) Walp.) plants: Integration of physiological and biochemical responses |
title_sort |
Assessment of selenium spatial distribution using μ-XFR in cowpea (Vigna unguiculata (L.) Walp.) plants: Integration of physiological and biochemical responses |
author |
Lanza, Maria Gabriela Dantas Bereta [UNESP] |
author_facet |
Lanza, Maria Gabriela Dantas Bereta [UNESP] Silva, Vinícius Martins [UNESP] Montanha, Gabriel Sgarbiero Lavres, José Pereira de Carvalho, Hudson Wallace Reis, André Rodrigues dos [UNESP] |
author_role |
author |
author2 |
Silva, Vinícius Martins [UNESP] Montanha, Gabriel Sgarbiero Lavres, José Pereira de Carvalho, Hudson Wallace Reis, André Rodrigues dos [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Lanza, Maria Gabriela Dantas Bereta [UNESP] Silva, Vinícius Martins [UNESP] Montanha, Gabriel Sgarbiero Lavres, José Pereira de Carvalho, Hudson Wallace Reis, André Rodrigues dos [UNESP] |
dc.subject.por.fl_str_mv |
Agronomic biofortification Antioxidant metabolism Scanning electron microscopy Selenium Vigna unguiculata X-ray fluorescence microscopy |
topic |
Agronomic biofortification Antioxidant metabolism Scanning electron microscopy Selenium Vigna unguiculata X-ray fluorescence microscopy |
description |
Low concentrations of selenium (Se) are beneficial for plant growth. Foliar Se application at high concentrations is toxic to plants due to the formation of reactive oxygen species (ROS). This study characterized Se toxicity symptoms using X-ray fluorescence (XRF) technique in response to foliar Se application in cowpea plants. Five Se concentrations (0, 10, 25, 50, 100 e 150 g ha−1) were sprayed on leaves as sodium selenate. The visual symptoms of Se toxicity in cowpea leaves were separated into two stages: I) necrotic points with an irregular distribution and internerval chlorosis at the leaf limb border (50–100 g ha−1); II) total chlorosis with the formation of dark brown necrotic lesions (150 g ha−1). Foliar Se application at 50 g ha−1 increased photosynthetic pigments and yield. Ultrastructural analyses showed that Se foliar application above 50 g ha−1 disarranged the upper epidermis of cowpea leaves. Furthermore, Se application above 100 g ha−1 significantly increased the hydrogen peroxide concentration and lipid peroxidation inducing necrotic leaf lesions. Mapping of the elements in leaves using the XRF revealed high Se intensity, specifically in leaf necrotic lesions accompanied by calcium (Ca) as a possible attenuating mechanism of plant stress. The distribution of Se intensities in the seeds was homogeneous, without specific accumulation sites. Phosphorus (P) and sulfur (S) were found primarily located in the embryonic region. Understanding the factors involved in Se accumulation and its interaction with Ca support new preventive measurement technologies to prevent Se toxicity in plants. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-06-25T10:10:59Z 2021-06-25T10:10:59Z 2021-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ecoenv.2020.111216 Ecotoxicology and Environmental Safety, v. 207. 1090-2414 0147-6513 http://hdl.handle.net/11449/205166 10.1016/j.ecoenv.2020.111216 2-s2.0-85090322594 |
url |
http://dx.doi.org/10.1016/j.ecoenv.2020.111216 http://hdl.handle.net/11449/205166 |
identifier_str_mv |
Ecotoxicology and Environmental Safety, v. 207. 1090-2414 0147-6513 10.1016/j.ecoenv.2020.111216 2-s2.0-85090322594 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Ecotoxicology and Environmental Safety |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129432278269952 |