Experimental hyperthyroidism decreases gene expression and serum levels of adipokines in obesity

Detalhes bibliográficos
Autor(a) principal: Luvizotto, Renata de Azevedo Melo
Data de Publicação: 2012
Outros Autores: Nascimento, André Ferreira Do, Síbio, Maria Teresa De, Olímpio, Regiane Marques Castro, Conde, Sandro José, Lima-Leopoldo, Ana Paula, Leopoldo, André Soares, Cicogna, Antonio Carlos [UNESP], Nogueira, Célia Regina [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1100/2012/780890
http://hdl.handle.net/11449/73389
Resumo: Aims. To analyze the influence of hyperthyroidism on the gene expression and serum concentration of leptin, resistin, and adiponectin in obese animals. Main Methods. Male Wistar rats were randomly divided into two groups: control (C)fed with commercial chow ad libitumand obese (OB)fed with a hypercaloric diet. After group characterization, the OB rats continued receiving a hypercaloric diet and were randomized into two groups: obese animals (OB) and obese with 25g triiodothyronine (T3)/100BW (OT). The T3 dose was administered every day for the last 2 weeks of the study. After 30 weeks the animals were euthanized. Samples of blood and adipose tissue were collected for biochemical and hormonal analyses as well as gene expression of leptin, resistin, and adiponectin. Results. T3 treatment was effective, increasing fT3 levels and decreasing fT4 and TSH serum concentration. Administration of T3 promotes weight loss, decreases all fat deposits, and diminishes serum levels of leptin, resistin, and adiponectin by reducing their gene expression. Conclusions. Our results suggest that T3 modulate serum and gene expression levels of leptin, resistin, and adiponectin in experimental model of obesity, providing new insights regarding the relationship between T3 and adipokines in obesity. Copyright © 2012 Renata de Azevedo Melo Luvizotto et al.
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spelling Experimental hyperthyroidism decreases gene expression and serum levels of adipokines in obesityadipocytokineadiponectinfatty acidglucoseleptinliothyronineresistinthyrotropinthyroxinetriacylglyceroladipose tissueanimal euthanasiaanimal experimentanimal tissuebiochemistryblood samplingbody fatcontrolled studydietexperimental hyperthyroidismfree liothyronine indexfree thyroxine indexgene expressionhormone determinationhypercaloric diethyperthyroidismmalenonhumanobesityratweight reductionanimalbiosynthesisbloodbody weightdisease modelgene expression regulationhomeostasismetabolismrandomizationWistar ratAdiponectinAdipose TissueAnimalsBody WeightDisease Models, AnimalGene Expression RegulationHomeostasisHyperthyroidismLeptinMaleObesityRandom AllocationRatsRats, WistarResistinThyrotropinThyroxineTriiodothyronineAims. To analyze the influence of hyperthyroidism on the gene expression and serum concentration of leptin, resistin, and adiponectin in obese animals. Main Methods. Male Wistar rats were randomly divided into two groups: control (C)fed with commercial chow ad libitumand obese (OB)fed with a hypercaloric diet. After group characterization, the OB rats continued receiving a hypercaloric diet and were randomized into two groups: obese animals (OB) and obese with 25g triiodothyronine (T3)/100BW (OT). The T3 dose was administered every day for the last 2 weeks of the study. After 30 weeks the animals were euthanized. Samples of blood and adipose tissue were collected for biochemical and hormonal analyses as well as gene expression of leptin, resistin, and adiponectin. Results. T3 treatment was effective, increasing fT3 levels and decreasing fT4 and TSH serum concentration. Administration of T3 promotes weight loss, decreases all fat deposits, and diminishes serum levels of leptin, resistin, and adiponectin by reducing their gene expression. Conclusions. Our results suggest that T3 modulate serum and gene expression levels of leptin, resistin, and adiponectin in experimental model of obesity, providing new insights regarding the relationship between T3 and adipokines in obesity. Copyright © 2012 Renata de Azevedo Melo Luvizotto et al.Department of Internal Medicine Botucatu School of Medicine University of São Paulo State, 18618-000 Botucatu, SPDepartment of Sports Center of Physical Education and Sports Federal University of Espirito Santo (UFES), 29075-910 Vitória, ESUniversidade Estadual Paulista (Unesp)Universidade Federal do Espírito Santo (UFES)Luvizotto, Renata de Azevedo MeloNascimento, André Ferreira DoSíbio, Maria Teresa DeOlímpio, Regiane Marques CastroConde, Sandro JoséLima-Leopoldo, Ana PaulaLeopoldo, André SoaresCicogna, Antonio Carlos [UNESP]Nogueira, Célia Regina [UNESP]2014-05-27T11:26:51Z2014-05-27T11:26:51Z2012-06-21info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1100/2012/780890The Scientific World Journal, v. 2012.1537-744Xhttp://hdl.handle.net/11449/7338910.1100/2012/7808902-s2.0-848623339292-s2.0-84862333929.pdf9418970103564137Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengThe Scientific World Journalinfo:eu-repo/semantics/openAccess2024-08-14T17:23:43Zoai:repositorio.unesp.br:11449/73389Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:23:43Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Experimental hyperthyroidism decreases gene expression and serum levels of adipokines in obesity
title Experimental hyperthyroidism decreases gene expression and serum levels of adipokines in obesity
spellingShingle Experimental hyperthyroidism decreases gene expression and serum levels of adipokines in obesity
Luvizotto, Renata de Azevedo Melo
adipocytokine
adiponectin
fatty acid
glucose
leptin
liothyronine
resistin
thyrotropin
thyroxine
triacylglycerol
adipose tissue
animal euthanasia
animal experiment
animal tissue
biochemistry
blood sampling
body fat
controlled study
diet
experimental hyperthyroidism
free liothyronine index
free thyroxine index
gene expression
hormone determination
hypercaloric diet
hyperthyroidism
male
nonhuman
obesity
rat
weight reduction
animal
biosynthesis
blood
body weight
disease model
gene expression regulation
homeostasis
metabolism
randomization
Wistar rat
Adiponectin
Adipose Tissue
Animals
Body Weight
Disease Models, Animal
Gene Expression Regulation
Homeostasis
Hyperthyroidism
Leptin
Male
Obesity
Random Allocation
Rats
Rats, Wistar
Resistin
Thyrotropin
Thyroxine
Triiodothyronine
title_short Experimental hyperthyroidism decreases gene expression and serum levels of adipokines in obesity
title_full Experimental hyperthyroidism decreases gene expression and serum levels of adipokines in obesity
title_fullStr Experimental hyperthyroidism decreases gene expression and serum levels of adipokines in obesity
title_full_unstemmed Experimental hyperthyroidism decreases gene expression and serum levels of adipokines in obesity
title_sort Experimental hyperthyroidism decreases gene expression and serum levels of adipokines in obesity
author Luvizotto, Renata de Azevedo Melo
author_facet Luvizotto, Renata de Azevedo Melo
Nascimento, André Ferreira Do
Síbio, Maria Teresa De
Olímpio, Regiane Marques Castro
Conde, Sandro José
Lima-Leopoldo, Ana Paula
Leopoldo, André Soares
Cicogna, Antonio Carlos [UNESP]
Nogueira, Célia Regina [UNESP]
author_role author
author2 Nascimento, André Ferreira Do
Síbio, Maria Teresa De
Olímpio, Regiane Marques Castro
Conde, Sandro José
Lima-Leopoldo, Ana Paula
Leopoldo, André Soares
Cicogna, Antonio Carlos [UNESP]
Nogueira, Célia Regina [UNESP]
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade Federal do Espírito Santo (UFES)
dc.contributor.author.fl_str_mv Luvizotto, Renata de Azevedo Melo
Nascimento, André Ferreira Do
Síbio, Maria Teresa De
Olímpio, Regiane Marques Castro
Conde, Sandro José
Lima-Leopoldo, Ana Paula
Leopoldo, André Soares
Cicogna, Antonio Carlos [UNESP]
Nogueira, Célia Regina [UNESP]
dc.subject.por.fl_str_mv adipocytokine
adiponectin
fatty acid
glucose
leptin
liothyronine
resistin
thyrotropin
thyroxine
triacylglycerol
adipose tissue
animal euthanasia
animal experiment
animal tissue
biochemistry
blood sampling
body fat
controlled study
diet
experimental hyperthyroidism
free liothyronine index
free thyroxine index
gene expression
hormone determination
hypercaloric diet
hyperthyroidism
male
nonhuman
obesity
rat
weight reduction
animal
biosynthesis
blood
body weight
disease model
gene expression regulation
homeostasis
metabolism
randomization
Wistar rat
Adiponectin
Adipose Tissue
Animals
Body Weight
Disease Models, Animal
Gene Expression Regulation
Homeostasis
Hyperthyroidism
Leptin
Male
Obesity
Random Allocation
Rats
Rats, Wistar
Resistin
Thyrotropin
Thyroxine
Triiodothyronine
topic adipocytokine
adiponectin
fatty acid
glucose
leptin
liothyronine
resistin
thyrotropin
thyroxine
triacylglycerol
adipose tissue
animal euthanasia
animal experiment
animal tissue
biochemistry
blood sampling
body fat
controlled study
diet
experimental hyperthyroidism
free liothyronine index
free thyroxine index
gene expression
hormone determination
hypercaloric diet
hyperthyroidism
male
nonhuman
obesity
rat
weight reduction
animal
biosynthesis
blood
body weight
disease model
gene expression regulation
homeostasis
metabolism
randomization
Wistar rat
Adiponectin
Adipose Tissue
Animals
Body Weight
Disease Models, Animal
Gene Expression Regulation
Homeostasis
Hyperthyroidism
Leptin
Male
Obesity
Random Allocation
Rats
Rats, Wistar
Resistin
Thyrotropin
Thyroxine
Triiodothyronine
description Aims. To analyze the influence of hyperthyroidism on the gene expression and serum concentration of leptin, resistin, and adiponectin in obese animals. Main Methods. Male Wistar rats were randomly divided into two groups: control (C)fed with commercial chow ad libitumand obese (OB)fed with a hypercaloric diet. After group characterization, the OB rats continued receiving a hypercaloric diet and were randomized into two groups: obese animals (OB) and obese with 25g triiodothyronine (T3)/100BW (OT). The T3 dose was administered every day for the last 2 weeks of the study. After 30 weeks the animals were euthanized. Samples of blood and adipose tissue were collected for biochemical and hormonal analyses as well as gene expression of leptin, resistin, and adiponectin. Results. T3 treatment was effective, increasing fT3 levels and decreasing fT4 and TSH serum concentration. Administration of T3 promotes weight loss, decreases all fat deposits, and diminishes serum levels of leptin, resistin, and adiponectin by reducing their gene expression. Conclusions. Our results suggest that T3 modulate serum and gene expression levels of leptin, resistin, and adiponectin in experimental model of obesity, providing new insights regarding the relationship between T3 and adipokines in obesity. Copyright © 2012 Renata de Azevedo Melo Luvizotto et al.
publishDate 2012
dc.date.none.fl_str_mv 2012-06-21
2014-05-27T11:26:51Z
2014-05-27T11:26:51Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1100/2012/780890
The Scientific World Journal, v. 2012.
1537-744X
http://hdl.handle.net/11449/73389
10.1100/2012/780890
2-s2.0-84862333929
2-s2.0-84862333929.pdf
9418970103564137
url http://dx.doi.org/10.1100/2012/780890
http://hdl.handle.net/11449/73389
identifier_str_mv The Scientific World Journal, v. 2012.
1537-744X
10.1100/2012/780890
2-s2.0-84862333929
2-s2.0-84862333929.pdf
9418970103564137
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv The Scientific World Journal
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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