Repetitive DNA sequences and evolution of ZZ/ZW sex chromosomes in characidium (teleostei: characiformes)
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1371/journal.pone.0137231 http://hdl.handle.net/11449/131239 |
Resumo: | Characidium constitutes an interesting model for cytogenetic studies, since a large degree of karyotype variation has been detected in this group, like the presence/absence of sex and supernumerary chromosomes and variable distribution of repetitive sequences in different species/populations. In this study, we performed a comparative cytogenetic analysis in 13 Characidium species collected at different South American river basins in order to investigate the karyotype diversification in this group. Chromosome analyses involved the karyotype characterization, cytogenetic mapping of repetitive DNA sequences and cross-species chromosome painting using a W-specific probe obtained in a previous study from Characidium gomesi. Our results evidenced a conserved diploid chromosome number of 2n = 50, and almost all the species exhibited homeologous ZZ/ZW sex chromosomes in different stages of differentiation, except C. cf. zebra, C. tenue, C. xavante and C. stigmosum. Notably, some ZZ/ZW sex chromosomes showed 5S and/or 18S rDNA clusters, while no U2 snDNA sites could be detected in the sex chromosomes, being restricted to a single chromosome pair in almost all the analyzed species. In addition, the species Characidium sp. aff. C. vidali showed B chromosomes with an inter-individual variation of 1 to 4 supernumerary chromosomes per cell. Notably, these B chromosomes share sequences with the W-specific probe, providing insights about their origin. Results presented here further confirm the extensive karyotype diversity within Characidium in contrast with a conserved diploid chromosome number. Such chromosome differences seem to constitute a significant reproductive barrier, since several sympatric Characidium species had been described during the last few years and no interespecific hybrids were found. |
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Repetitive DNA sequences and evolution of ZZ/ZW sex chromosomes in characidium (teleostei: characiformes)Characidium constitutes an interesting model for cytogenetic studies, since a large degree of karyotype variation has been detected in this group, like the presence/absence of sex and supernumerary chromosomes and variable distribution of repetitive sequences in different species/populations. In this study, we performed a comparative cytogenetic analysis in 13 Characidium species collected at different South American river basins in order to investigate the karyotype diversification in this group. Chromosome analyses involved the karyotype characterization, cytogenetic mapping of repetitive DNA sequences and cross-species chromosome painting using a W-specific probe obtained in a previous study from Characidium gomesi. Our results evidenced a conserved diploid chromosome number of 2n = 50, and almost all the species exhibited homeologous ZZ/ZW sex chromosomes in different stages of differentiation, except C. cf. zebra, C. tenue, C. xavante and C. stigmosum. Notably, some ZZ/ZW sex chromosomes showed 5S and/or 18S rDNA clusters, while no U2 snDNA sites could be detected in the sex chromosomes, being restricted to a single chromosome pair in almost all the analyzed species. In addition, the species Characidium sp. aff. C. vidali showed B chromosomes with an inter-individual variation of 1 to 4 supernumerary chromosomes per cell. Notably, these B chromosomes share sequences with the W-specific probe, providing insights about their origin. Results presented here further confirm the extensive karyotype diversity within Characidium in contrast with a conserved diploid chromosome number. Such chromosome differences seem to constitute a significant reproductive barrier, since several sympatric Characidium species had been described during the last few years and no interespecific hybrids were found.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenadoria de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista (UNESP), Instituto de Biociências de Botucatu/IBB, Departamento de Morfologia, Botucatu, SP, BrazilUniversidade Estadual de Ponta Grossa (UEPG), Departamento de Biologia Estrutural, Molecular e Genética, Ponta Grossa, PR, BrazilUniversidade Estadual Paulista (UNESP), Instituto de Biociências de Botucatu/IBB, Departamento de Morfologia, Botucatu, SP, BrazilPublic Library ScienceUniversidade Estadual Paulista (Unesp)Universidade Estadual de Ponta Grossa (UEPG)Scacchetti, Priscilla Cardim [UNESP]Utsunomia, Ricardo [UNESP]Pansonato-Alves, José Carlos [UNESP]Silva, Guilherme José da Costa [UNESP]Vicari, Marcelo RicardoArtoni, Roberto FerreiraOliveira, Claudio [UNESP]Foresti, Fausto [UNESP]2015-12-07T15:32:57Z2015-12-07T15:32:57Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1371/journal.pone.0137231Plos One, v. 10, n. 9, 2015.1932-6203http://hdl.handle.net/11449/13123910.1371/journal.pone.0137231PMC4570811.pdf080479394484636726372604PMC4570811PubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengPlos One2.7661,164info:eu-repo/semantics/openAccess2024-01-15T06:18:14Zoai:repositorio.unesp.br:11449/131239Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:00:50.022094Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Repetitive DNA sequences and evolution of ZZ/ZW sex chromosomes in characidium (teleostei: characiformes) |
title |
Repetitive DNA sequences and evolution of ZZ/ZW sex chromosomes in characidium (teleostei: characiformes) |
spellingShingle |
Repetitive DNA sequences and evolution of ZZ/ZW sex chromosomes in characidium (teleostei: characiformes) Scacchetti, Priscilla Cardim [UNESP] |
title_short |
Repetitive DNA sequences and evolution of ZZ/ZW sex chromosomes in characidium (teleostei: characiformes) |
title_full |
Repetitive DNA sequences and evolution of ZZ/ZW sex chromosomes in characidium (teleostei: characiformes) |
title_fullStr |
Repetitive DNA sequences and evolution of ZZ/ZW sex chromosomes in characidium (teleostei: characiformes) |
title_full_unstemmed |
Repetitive DNA sequences and evolution of ZZ/ZW sex chromosomes in characidium (teleostei: characiformes) |
title_sort |
Repetitive DNA sequences and evolution of ZZ/ZW sex chromosomes in characidium (teleostei: characiformes) |
author |
Scacchetti, Priscilla Cardim [UNESP] |
author_facet |
Scacchetti, Priscilla Cardim [UNESP] Utsunomia, Ricardo [UNESP] Pansonato-Alves, José Carlos [UNESP] Silva, Guilherme José da Costa [UNESP] Vicari, Marcelo Ricardo Artoni, Roberto Ferreira Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
author_role |
author |
author2 |
Utsunomia, Ricardo [UNESP] Pansonato-Alves, José Carlos [UNESP] Silva, Guilherme José da Costa [UNESP] Vicari, Marcelo Ricardo Artoni, Roberto Ferreira Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Ponta Grossa (UEPG) |
dc.contributor.author.fl_str_mv |
Scacchetti, Priscilla Cardim [UNESP] Utsunomia, Ricardo [UNESP] Pansonato-Alves, José Carlos [UNESP] Silva, Guilherme José da Costa [UNESP] Vicari, Marcelo Ricardo Artoni, Roberto Ferreira Oliveira, Claudio [UNESP] Foresti, Fausto [UNESP] |
description |
Characidium constitutes an interesting model for cytogenetic studies, since a large degree of karyotype variation has been detected in this group, like the presence/absence of sex and supernumerary chromosomes and variable distribution of repetitive sequences in different species/populations. In this study, we performed a comparative cytogenetic analysis in 13 Characidium species collected at different South American river basins in order to investigate the karyotype diversification in this group. Chromosome analyses involved the karyotype characterization, cytogenetic mapping of repetitive DNA sequences and cross-species chromosome painting using a W-specific probe obtained in a previous study from Characidium gomesi. Our results evidenced a conserved diploid chromosome number of 2n = 50, and almost all the species exhibited homeologous ZZ/ZW sex chromosomes in different stages of differentiation, except C. cf. zebra, C. tenue, C. xavante and C. stigmosum. Notably, some ZZ/ZW sex chromosomes showed 5S and/or 18S rDNA clusters, while no U2 snDNA sites could be detected in the sex chromosomes, being restricted to a single chromosome pair in almost all the analyzed species. In addition, the species Characidium sp. aff. C. vidali showed B chromosomes with an inter-individual variation of 1 to 4 supernumerary chromosomes per cell. Notably, these B chromosomes share sequences with the W-specific probe, providing insights about their origin. Results presented here further confirm the extensive karyotype diversity within Characidium in contrast with a conserved diploid chromosome number. Such chromosome differences seem to constitute a significant reproductive barrier, since several sympatric Characidium species had been described during the last few years and no interespecific hybrids were found. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-07T15:32:57Z 2015-12-07T15:32:57Z 2015 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1371/journal.pone.0137231 Plos One, v. 10, n. 9, 2015. 1932-6203 http://hdl.handle.net/11449/131239 10.1371/journal.pone.0137231 PMC4570811.pdf 0804793944846367 26372604 PMC4570811 |
url |
http://dx.doi.org/10.1371/journal.pone.0137231 http://hdl.handle.net/11449/131239 |
identifier_str_mv |
Plos One, v. 10, n. 9, 2015. 1932-6203 10.1371/journal.pone.0137231 PMC4570811.pdf 0804793944846367 26372604 PMC4570811 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Plos One 2.766 1,164 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Public Library Science |
publisher.none.fl_str_mv |
Public Library Science |
dc.source.none.fl_str_mv |
PubMed reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
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UNESP |
reponame_str |
Repositório Institucional da UNESP |
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Repositório Institucional da UNESP |
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Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
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1808129481715482624 |