Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.archoralbio.2021.105202 http://hdl.handle.net/11449/229087 |
Resumo: | Objective: To evaluate formulations of 1 % silver (Ag) nanoparticles for treating traumatic lesions induced in the oral mucosa of rats, because these lesions are commonly observed in the dental clinic, and their therapeutic forms are scarce. Methods: Wistar rats were punch-injured (two circular fragments, 4.0 mm in diameter) in the oral mucosa (one on each side), and were treated topically (twice per week) with the treatments/groups including: no injury, control, vehicle, diluted Ag, soluble Ag, and solid Ag. On the 2nd, 7th, and 14th days postinjury, biopsies were collected for immunohistochemistry and biochemical analysis. Results: The group diluted Ag revealed a higher level of inflammatory infiltrate on the 2nd day, whereas solid Ag presented lower levels. The Ag solid group presented higher IL-1β on the 2nd day and increased IL-10 and TGF-β1 throughout the follow-up. Moreover, all three Ag groups presented lower levels of oxidative stress markers and, on the 7th day, the diluted Ag and solid Ag groups revealed higher antioxidants. Diluted Ag and soluble Ag groups presented greater blood vessels proliferation, whereas soluble Ag and solid Ag groups revealed greater VEGF on the 2nd and 14th days. Furthermore, all three Ag groups were highlighted during fibroplasia, although collagenesis was similar to that observed in the control group. Conclusions: Although diluted Ag was noticeable for its important angiogenesis and fibroplasia, solid Ag was the most suitable formulation in healing oral lesions as it efficiently controlled inflammation and oxidative stress, thus favoring angiogenesis and tissue formation. |
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Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of ratsAngiogenesisAntioxidantFormulationInflammationOral woundsSilver nanoparticleObjective: To evaluate formulations of 1 % silver (Ag) nanoparticles for treating traumatic lesions induced in the oral mucosa of rats, because these lesions are commonly observed in the dental clinic, and their therapeutic forms are scarce. Methods: Wistar rats were punch-injured (two circular fragments, 4.0 mm in diameter) in the oral mucosa (one on each side), and were treated topically (twice per week) with the treatments/groups including: no injury, control, vehicle, diluted Ag, soluble Ag, and solid Ag. On the 2nd, 7th, and 14th days postinjury, biopsies were collected for immunohistochemistry and biochemical analysis. Results: The group diluted Ag revealed a higher level of inflammatory infiltrate on the 2nd day, whereas solid Ag presented lower levels. The Ag solid group presented higher IL-1β on the 2nd day and increased IL-10 and TGF-β1 throughout the follow-up. Moreover, all three Ag groups presented lower levels of oxidative stress markers and, on the 7th day, the diluted Ag and solid Ag groups revealed higher antioxidants. Diluted Ag and soluble Ag groups presented greater blood vessels proliferation, whereas soluble Ag and solid Ag groups revealed greater VEGF on the 2nd and 14th days. Furthermore, all three Ag groups were highlighted during fibroplasia, although collagenesis was similar to that observed in the control group. Conclusions: Although diluted Ag was noticeable for its important angiogenesis and fibroplasia, solid Ag was the most suitable formulation in healing oral lesions as it efficiently controlled inflammation and oxidative stress, thus favoring angiogenesis and tissue formation.Graduate Program in Biomedical Sciences University Center of Herminio Ometto Foundation – FHO, Dr. Maximiliano Baruto Ave, 500. Jardim UniversitarioLaboratory of Magnetic Materials and Colloids Department of Physical Chemistry Institute of Chemistry Sao Paulo State University (UNESP), Prof. Francisco Degni Ave, 55. Jardim QuitandinhaLaboratory of Experimental Physiopathology Graduate Program in Science of Health Universidade do Extremo Sul Catarinense - UNESC, Universitaria Ave, 1105. Universitario, Bloco S - Room 017Graduate Program in Odontology University Center of Herminio Ometto Foundation – FHO, Dr. Maximiliano Baruto Ave, 500. Jardim UniversitarioLaboratory of Magnetic Materials and Colloids Department of Physical Chemistry Institute of Chemistry Sao Paulo State University (UNESP), Prof. Francisco Degni Ave, 55. Jardim QuitandinhaUniversity Center of Herminio Ometto Foundation – FHOUniversidade Estadual Paulista (UNESP)Universidade do Extremo Sul Catarinense - UNESCCurtolo, GabriellaAraújo, Jaqueline de PaulaLima, Joyce AlessandraBrandt, João Victor [UNESP]Bittencourt, João Vitor SilvanoVenturini, Ligia MilanezSilveira, Paulo Cesar LockRogers, SylviaFranzini, Cristina Mariade Goes, Vivian Fernandes FurlettiAndrade, Thiago Antônio Moretti2022-04-29T08:30:18Z2022-04-29T08:30:18Z2021-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.archoralbio.2021.105202Archives of Oral Biology, v. 129.1879-15060003-9969http://hdl.handle.net/11449/22908710.1016/j.archoralbio.2021.1052022-s2.0-85109197567Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biologyinfo:eu-repo/semantics/openAccess2022-04-29T08:30:18Zoai:repositorio.unesp.br:11449/229087Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:13:40.424871Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats |
title |
Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats |
spellingShingle |
Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats Curtolo, Gabriella Angiogenesis Antioxidant Formulation Inflammation Oral wounds Silver nanoparticle |
title_short |
Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats |
title_full |
Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats |
title_fullStr |
Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats |
title_full_unstemmed |
Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats |
title_sort |
Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats |
author |
Curtolo, Gabriella |
author_facet |
Curtolo, Gabriella Araújo, Jaqueline de Paula Lima, Joyce Alessandra Brandt, João Victor [UNESP] Bittencourt, João Vitor Silvano Venturini, Ligia Milanez Silveira, Paulo Cesar Lock Rogers, Sylvia Franzini, Cristina Maria de Goes, Vivian Fernandes Furletti Andrade, Thiago Antônio Moretti |
author_role |
author |
author2 |
Araújo, Jaqueline de Paula Lima, Joyce Alessandra Brandt, João Victor [UNESP] Bittencourt, João Vitor Silvano Venturini, Ligia Milanez Silveira, Paulo Cesar Lock Rogers, Sylvia Franzini, Cristina Maria de Goes, Vivian Fernandes Furletti Andrade, Thiago Antônio Moretti |
author2_role |
author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
University Center of Herminio Ometto Foundation – FHO Universidade Estadual Paulista (UNESP) Universidade do Extremo Sul Catarinense - UNESC |
dc.contributor.author.fl_str_mv |
Curtolo, Gabriella Araújo, Jaqueline de Paula Lima, Joyce Alessandra Brandt, João Victor [UNESP] Bittencourt, João Vitor Silvano Venturini, Ligia Milanez Silveira, Paulo Cesar Lock Rogers, Sylvia Franzini, Cristina Maria de Goes, Vivian Fernandes Furletti Andrade, Thiago Antônio Moretti |
dc.subject.por.fl_str_mv |
Angiogenesis Antioxidant Formulation Inflammation Oral wounds Silver nanoparticle |
topic |
Angiogenesis Antioxidant Formulation Inflammation Oral wounds Silver nanoparticle |
description |
Objective: To evaluate formulations of 1 % silver (Ag) nanoparticles for treating traumatic lesions induced in the oral mucosa of rats, because these lesions are commonly observed in the dental clinic, and their therapeutic forms are scarce. Methods: Wistar rats were punch-injured (two circular fragments, 4.0 mm in diameter) in the oral mucosa (one on each side), and were treated topically (twice per week) with the treatments/groups including: no injury, control, vehicle, diluted Ag, soluble Ag, and solid Ag. On the 2nd, 7th, and 14th days postinjury, biopsies were collected for immunohistochemistry and biochemical analysis. Results: The group diluted Ag revealed a higher level of inflammatory infiltrate on the 2nd day, whereas solid Ag presented lower levels. The Ag solid group presented higher IL-1β on the 2nd day and increased IL-10 and TGF-β1 throughout the follow-up. Moreover, all three Ag groups presented lower levels of oxidative stress markers and, on the 7th day, the diluted Ag and solid Ag groups revealed higher antioxidants. Diluted Ag and soluble Ag groups presented greater blood vessels proliferation, whereas soluble Ag and solid Ag groups revealed greater VEGF on the 2nd and 14th days. Furthermore, all three Ag groups were highlighted during fibroplasia, although collagenesis was similar to that observed in the control group. Conclusions: Although diluted Ag was noticeable for its important angiogenesis and fibroplasia, solid Ag was the most suitable formulation in healing oral lesions as it efficiently controlled inflammation and oxidative stress, thus favoring angiogenesis and tissue formation. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-09-01 2022-04-29T08:30:18Z 2022-04-29T08:30:18Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.archoralbio.2021.105202 Archives of Oral Biology, v. 129. 1879-1506 0003-9969 http://hdl.handle.net/11449/229087 10.1016/j.archoralbio.2021.105202 2-s2.0-85109197567 |
url |
http://dx.doi.org/10.1016/j.archoralbio.2021.105202 http://hdl.handle.net/11449/229087 |
identifier_str_mv |
Archives of Oral Biology, v. 129. 1879-1506 0003-9969 10.1016/j.archoralbio.2021.105202 2-s2.0-85109197567 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Archives of Oral Biology |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128621776207872 |