Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats

Detalhes bibliográficos
Autor(a) principal: Curtolo, Gabriella
Data de Publicação: 2021
Outros Autores: Araújo, Jaqueline de Paula, Lima, Joyce Alessandra, Brandt, João Victor [UNESP], Bittencourt, João Vitor Silvano, Venturini, Ligia Milanez, Silveira, Paulo Cesar Lock, Rogers, Sylvia, Franzini, Cristina Maria, de Goes, Vivian Fernandes Furletti, Andrade, Thiago Antônio Moretti
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.archoralbio.2021.105202
http://hdl.handle.net/11449/229087
Resumo: Objective: To evaluate formulations of 1 % silver (Ag) nanoparticles for treating traumatic lesions induced in the oral mucosa of rats, because these lesions are commonly observed in the dental clinic, and their therapeutic forms are scarce. Methods: Wistar rats were punch-injured (two circular fragments, 4.0 mm in diameter) in the oral mucosa (one on each side), and were treated topically (twice per week) with the treatments/groups including: no injury, control, vehicle, diluted Ag, soluble Ag, and solid Ag. On the 2nd, 7th, and 14th days postinjury, biopsies were collected for immunohistochemistry and biochemical analysis. Results: The group diluted Ag revealed a higher level of inflammatory infiltrate on the 2nd day, whereas solid Ag presented lower levels. The Ag solid group presented higher IL-1β on the 2nd day and increased IL-10 and TGF-β1 throughout the follow-up. Moreover, all three Ag groups presented lower levels of oxidative stress markers and, on the 7th day, the diluted Ag and solid Ag groups revealed higher antioxidants. Diluted Ag and soluble Ag groups presented greater blood vessels proliferation, whereas soluble Ag and solid Ag groups revealed greater VEGF on the 2nd and 14th days. Furthermore, all three Ag groups were highlighted during fibroplasia, although collagenesis was similar to that observed in the control group. Conclusions: Although diluted Ag was noticeable for its important angiogenesis and fibroplasia, solid Ag was the most suitable formulation in healing oral lesions as it efficiently controlled inflammation and oxidative stress, thus favoring angiogenesis and tissue formation.
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spelling Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of ratsAngiogenesisAntioxidantFormulationInflammationOral woundsSilver nanoparticleObjective: To evaluate formulations of 1 % silver (Ag) nanoparticles for treating traumatic lesions induced in the oral mucosa of rats, because these lesions are commonly observed in the dental clinic, and their therapeutic forms are scarce. Methods: Wistar rats were punch-injured (two circular fragments, 4.0 mm in diameter) in the oral mucosa (one on each side), and were treated topically (twice per week) with the treatments/groups including: no injury, control, vehicle, diluted Ag, soluble Ag, and solid Ag. On the 2nd, 7th, and 14th days postinjury, biopsies were collected for immunohistochemistry and biochemical analysis. Results: The group diluted Ag revealed a higher level of inflammatory infiltrate on the 2nd day, whereas solid Ag presented lower levels. The Ag solid group presented higher IL-1β on the 2nd day and increased IL-10 and TGF-β1 throughout the follow-up. Moreover, all three Ag groups presented lower levels of oxidative stress markers and, on the 7th day, the diluted Ag and solid Ag groups revealed higher antioxidants. Diluted Ag and soluble Ag groups presented greater blood vessels proliferation, whereas soluble Ag and solid Ag groups revealed greater VEGF on the 2nd and 14th days. Furthermore, all three Ag groups were highlighted during fibroplasia, although collagenesis was similar to that observed in the control group. Conclusions: Although diluted Ag was noticeable for its important angiogenesis and fibroplasia, solid Ag was the most suitable formulation in healing oral lesions as it efficiently controlled inflammation and oxidative stress, thus favoring angiogenesis and tissue formation.Graduate Program in Biomedical Sciences University Center of Herminio Ometto Foundation – FHO, Dr. Maximiliano Baruto Ave, 500. Jardim UniversitarioLaboratory of Magnetic Materials and Colloids Department of Physical Chemistry Institute of Chemistry Sao Paulo State University (UNESP), Prof. Francisco Degni Ave, 55. Jardim QuitandinhaLaboratory of Experimental Physiopathology Graduate Program in Science of Health Universidade do Extremo Sul Catarinense - UNESC, Universitaria Ave, 1105. Universitario, Bloco S - Room 017Graduate Program in Odontology University Center of Herminio Ometto Foundation – FHO, Dr. Maximiliano Baruto Ave, 500. Jardim UniversitarioLaboratory of Magnetic Materials and Colloids Department of Physical Chemistry Institute of Chemistry Sao Paulo State University (UNESP), Prof. Francisco Degni Ave, 55. Jardim QuitandinhaUniversity Center of Herminio Ometto Foundation – FHOUniversidade Estadual Paulista (UNESP)Universidade do Extremo Sul Catarinense - UNESCCurtolo, GabriellaAraújo, Jaqueline de PaulaLima, Joyce AlessandraBrandt, João Victor [UNESP]Bittencourt, João Vitor SilvanoVenturini, Ligia MilanezSilveira, Paulo Cesar LockRogers, SylviaFranzini, Cristina Mariade Goes, Vivian Fernandes FurlettiAndrade, Thiago Antônio Moretti2022-04-29T08:30:18Z2022-04-29T08:30:18Z2021-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.archoralbio.2021.105202Archives of Oral Biology, v. 129.1879-15060003-9969http://hdl.handle.net/11449/22908710.1016/j.archoralbio.2021.1052022-s2.0-85109197567Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengArchives of Oral Biologyinfo:eu-repo/semantics/openAccess2022-04-29T08:30:18Zoai:repositorio.unesp.br:11449/229087Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:13:40.424871Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats
title Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats
spellingShingle Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats
Curtolo, Gabriella
Angiogenesis
Antioxidant
Formulation
Inflammation
Oral wounds
Silver nanoparticle
title_short Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats
title_full Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats
title_fullStr Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats
title_full_unstemmed Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats
title_sort Silver nanoparticles formulations for healing traumatic injuries in oral mucosa of rats
author Curtolo, Gabriella
author_facet Curtolo, Gabriella
Araújo, Jaqueline de Paula
Lima, Joyce Alessandra
Brandt, João Victor [UNESP]
Bittencourt, João Vitor Silvano
Venturini, Ligia Milanez
Silveira, Paulo Cesar Lock
Rogers, Sylvia
Franzini, Cristina Maria
de Goes, Vivian Fernandes Furletti
Andrade, Thiago Antônio Moretti
author_role author
author2 Araújo, Jaqueline de Paula
Lima, Joyce Alessandra
Brandt, João Victor [UNESP]
Bittencourt, João Vitor Silvano
Venturini, Ligia Milanez
Silveira, Paulo Cesar Lock
Rogers, Sylvia
Franzini, Cristina Maria
de Goes, Vivian Fernandes Furletti
Andrade, Thiago Antônio Moretti
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv University Center of Herminio Ometto Foundation – FHO
Universidade Estadual Paulista (UNESP)
Universidade do Extremo Sul Catarinense - UNESC
dc.contributor.author.fl_str_mv Curtolo, Gabriella
Araújo, Jaqueline de Paula
Lima, Joyce Alessandra
Brandt, João Victor [UNESP]
Bittencourt, João Vitor Silvano
Venturini, Ligia Milanez
Silveira, Paulo Cesar Lock
Rogers, Sylvia
Franzini, Cristina Maria
de Goes, Vivian Fernandes Furletti
Andrade, Thiago Antônio Moretti
dc.subject.por.fl_str_mv Angiogenesis
Antioxidant
Formulation
Inflammation
Oral wounds
Silver nanoparticle
topic Angiogenesis
Antioxidant
Formulation
Inflammation
Oral wounds
Silver nanoparticle
description Objective: To evaluate formulations of 1 % silver (Ag) nanoparticles for treating traumatic lesions induced in the oral mucosa of rats, because these lesions are commonly observed in the dental clinic, and their therapeutic forms are scarce. Methods: Wistar rats were punch-injured (two circular fragments, 4.0 mm in diameter) in the oral mucosa (one on each side), and were treated topically (twice per week) with the treatments/groups including: no injury, control, vehicle, diluted Ag, soluble Ag, and solid Ag. On the 2nd, 7th, and 14th days postinjury, biopsies were collected for immunohistochemistry and biochemical analysis. Results: The group diluted Ag revealed a higher level of inflammatory infiltrate on the 2nd day, whereas solid Ag presented lower levels. The Ag solid group presented higher IL-1β on the 2nd day and increased IL-10 and TGF-β1 throughout the follow-up. Moreover, all three Ag groups presented lower levels of oxidative stress markers and, on the 7th day, the diluted Ag and solid Ag groups revealed higher antioxidants. Diluted Ag and soluble Ag groups presented greater blood vessels proliferation, whereas soluble Ag and solid Ag groups revealed greater VEGF on the 2nd and 14th days. Furthermore, all three Ag groups were highlighted during fibroplasia, although collagenesis was similar to that observed in the control group. Conclusions: Although diluted Ag was noticeable for its important angiogenesis and fibroplasia, solid Ag was the most suitable formulation in healing oral lesions as it efficiently controlled inflammation and oxidative stress, thus favoring angiogenesis and tissue formation.
publishDate 2021
dc.date.none.fl_str_mv 2021-09-01
2022-04-29T08:30:18Z
2022-04-29T08:30:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.archoralbio.2021.105202
Archives of Oral Biology, v. 129.
1879-1506
0003-9969
http://hdl.handle.net/11449/229087
10.1016/j.archoralbio.2021.105202
2-s2.0-85109197567
url http://dx.doi.org/10.1016/j.archoralbio.2021.105202
http://hdl.handle.net/11449/229087
identifier_str_mv Archives of Oral Biology, v. 129.
1879-1506
0003-9969
10.1016/j.archoralbio.2021.105202
2-s2.0-85109197567
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Archives of Oral Biology
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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