Bacterial cellulose membrane incorporated with silver nanoparticles for wound healing in animal model

Detalhes bibliográficos
Autor(a) principal: Munhoz, Lauriene Luiza S.
Data de Publicação: 2023
Outros Autores: Alves, Miriã Tonus O., Alves, Beatriz C., Nascimento, Maria Gabriela F.S., Sábio, Rafael M. [UNESP], Manieri, Karyn F. [UNESP], Barud, Hernane S., Esquisatto, Marcelo Augusto M., Aro, Andrea A., de Roch Casagrande, Laura, Silveira, Paulo Cesar Lock, Santos, Glaucia Maria T., Andrade, Thiago A.M., Caetano, Guilherme F.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1016/j.bbrc.2023.02.058
http://hdl.handle.net/11449/246947
Resumo: The bacterial cellulose membrane (CM) is a promising biomaterial due to its easy applicability and moist environment. Moreover, nanoscale silver compounds (AgNO3) are synthesized and incorporated into CMs to provide these biomaterials with antimicrobial activity for wound healing. This study aimed to evaluate the cell viability of CM incorporated with nanoscale silver compounds, determine the minimum inhibitory concentration (MIC) for Escherichia coli and Staphylococcus aureus, and its use on in vivo skin lesions. Wistar rats were divided according to treatment: untreated, CM (cellulose membrane), and AgCM (CM incorporated with silver nanoparticles). The euthanasia was performed on the 2nd, 7th, 14th, and 21st days to assess inflammation (myeloperoxidase-neutrophils, N-acetylglucosaminidase-macrophage, IL-1β, IL-10), oxidative stress (NO-nitric oxide, DCF-H2O2), oxidative damage (carbonyl: membrane's damage; sulfhydryl: membrane's integrity), antioxidants (superoxide dismutase; glutathione), angiogenesis, tissue formation (collagen, TGF-β1, smooth muscle α-actin, small decorin, and biglycan proteoglycans). The use of AgCM did not show toxicity, but antibacterial effect in vitro. Moreover, in vivo, AgCM provided balanced oxidative action, modulated the inflammatory profile due to the reduction of IL-1β level and increase in IL-10 level, in addition to increased angiogenesis and collagen formation. The results suggest the use of silver nanoparticles (AgCM) enhanced the CM properties by providing antibacterial properties, modulation the inflammatory phase, and consequently promotes the healing of skin lesions, which can be used clinically to treat injuries.
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spelling Bacterial cellulose membrane incorporated with silver nanoparticles for wound healing in animal modelBacterial celluloseBiomembraneInflammationOxidative stressSilver nanoparticlesTissue formationThe bacterial cellulose membrane (CM) is a promising biomaterial due to its easy applicability and moist environment. Moreover, nanoscale silver compounds (AgNO3) are synthesized and incorporated into CMs to provide these biomaterials with antimicrobial activity for wound healing. This study aimed to evaluate the cell viability of CM incorporated with nanoscale silver compounds, determine the minimum inhibitory concentration (MIC) for Escherichia coli and Staphylococcus aureus, and its use on in vivo skin lesions. Wistar rats were divided according to treatment: untreated, CM (cellulose membrane), and AgCM (CM incorporated with silver nanoparticles). The euthanasia was performed on the 2nd, 7th, 14th, and 21st days to assess inflammation (myeloperoxidase-neutrophils, N-acetylglucosaminidase-macrophage, IL-1β, IL-10), oxidative stress (NO-nitric oxide, DCF-H2O2), oxidative damage (carbonyl: membrane's damage; sulfhydryl: membrane's integrity), antioxidants (superoxide dismutase; glutathione), angiogenesis, tissue formation (collagen, TGF-β1, smooth muscle α-actin, small decorin, and biglycan proteoglycans). The use of AgCM did not show toxicity, but antibacterial effect in vitro. Moreover, in vivo, AgCM provided balanced oxidative action, modulated the inflammatory profile due to the reduction of IL-1β level and increase in IL-10 level, in addition to increased angiogenesis and collagen formation. The results suggest the use of silver nanoparticles (AgCM) enhanced the CM properties by providing antibacterial properties, modulation the inflammatory phase, and consequently promotes the healing of skin lesions, which can be used clinically to treat injuries.Graduate Program in Biomedical Sciences University Centre of Herminio Ometto Foundation, ArarasBioPolymer and Biomaterial Laboratory (BioPolMat) University of Araraquara (UNIARA), AraraquaraSchool of Pharmaceutical Sciences São Paulo State University – UNESP, AraraquaraLaboratory of Experimental Physiopathology Graduate Program in Science of Health Universidade do Extremo Sul Catarinense (UNESC), Santa CatarinaSchool of Pharmaceutical Sciences São Paulo State University – UNESP, AraraquaraUniversity Centre of Herminio Ometto FoundationUniversity of Araraquara (UNIARA)Universidade Estadual Paulista (UNESP)Universidade do Extremo Sul Catarinense (UNESC)Munhoz, Lauriene Luiza S.Alves, Miriã Tonus O.Alves, Beatriz C.Nascimento, Maria Gabriela F.S.Sábio, Rafael M. [UNESP]Manieri, Karyn F. [UNESP]Barud, Hernane S.Esquisatto, Marcelo Augusto M.Aro, Andrea A.de Roch Casagrande, LauraSilveira, Paulo Cesar LockSantos, Glaucia Maria T.Andrade, Thiago A.M.Caetano, Guilherme F.2023-07-29T12:54:52Z2023-07-29T12:54:52Z2023-04-30info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article47-54http://dx.doi.org/10.1016/j.bbrc.2023.02.058Biochemical and Biophysical Research Communications, v. 654, p. 47-54.1090-21040006-291Xhttp://hdl.handle.net/11449/24694710.1016/j.bbrc.2023.02.0582-s2.0-85149468065Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiochemical and Biophysical Research Communicationsinfo:eu-repo/semantics/openAccess2023-07-29T12:54:52Zoai:repositorio.unesp.br:11449/246947Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T13:54:46.989404Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Bacterial cellulose membrane incorporated with silver nanoparticles for wound healing in animal model
title Bacterial cellulose membrane incorporated with silver nanoparticles for wound healing in animal model
spellingShingle Bacterial cellulose membrane incorporated with silver nanoparticles for wound healing in animal model
Munhoz, Lauriene Luiza S.
Bacterial cellulose
Biomembrane
Inflammation
Oxidative stress
Silver nanoparticles
Tissue formation
title_short Bacterial cellulose membrane incorporated with silver nanoparticles for wound healing in animal model
title_full Bacterial cellulose membrane incorporated with silver nanoparticles for wound healing in animal model
title_fullStr Bacterial cellulose membrane incorporated with silver nanoparticles for wound healing in animal model
title_full_unstemmed Bacterial cellulose membrane incorporated with silver nanoparticles for wound healing in animal model
title_sort Bacterial cellulose membrane incorporated with silver nanoparticles for wound healing in animal model
author Munhoz, Lauriene Luiza S.
author_facet Munhoz, Lauriene Luiza S.
Alves, Miriã Tonus O.
Alves, Beatriz C.
Nascimento, Maria Gabriela F.S.
Sábio, Rafael M. [UNESP]
Manieri, Karyn F. [UNESP]
Barud, Hernane S.
Esquisatto, Marcelo Augusto M.
Aro, Andrea A.
de Roch Casagrande, Laura
Silveira, Paulo Cesar Lock
Santos, Glaucia Maria T.
Andrade, Thiago A.M.
Caetano, Guilherme F.
author_role author
author2 Alves, Miriã Tonus O.
Alves, Beatriz C.
Nascimento, Maria Gabriela F.S.
Sábio, Rafael M. [UNESP]
Manieri, Karyn F. [UNESP]
Barud, Hernane S.
Esquisatto, Marcelo Augusto M.
Aro, Andrea A.
de Roch Casagrande, Laura
Silveira, Paulo Cesar Lock
Santos, Glaucia Maria T.
Andrade, Thiago A.M.
Caetano, Guilherme F.
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv University Centre of Herminio Ometto Foundation
University of Araraquara (UNIARA)
Universidade Estadual Paulista (UNESP)
Universidade do Extremo Sul Catarinense (UNESC)
dc.contributor.author.fl_str_mv Munhoz, Lauriene Luiza S.
Alves, Miriã Tonus O.
Alves, Beatriz C.
Nascimento, Maria Gabriela F.S.
Sábio, Rafael M. [UNESP]
Manieri, Karyn F. [UNESP]
Barud, Hernane S.
Esquisatto, Marcelo Augusto M.
Aro, Andrea A.
de Roch Casagrande, Laura
Silveira, Paulo Cesar Lock
Santos, Glaucia Maria T.
Andrade, Thiago A.M.
Caetano, Guilherme F.
dc.subject.por.fl_str_mv Bacterial cellulose
Biomembrane
Inflammation
Oxidative stress
Silver nanoparticles
Tissue formation
topic Bacterial cellulose
Biomembrane
Inflammation
Oxidative stress
Silver nanoparticles
Tissue formation
description The bacterial cellulose membrane (CM) is a promising biomaterial due to its easy applicability and moist environment. Moreover, nanoscale silver compounds (AgNO3) are synthesized and incorporated into CMs to provide these biomaterials with antimicrobial activity for wound healing. This study aimed to evaluate the cell viability of CM incorporated with nanoscale silver compounds, determine the minimum inhibitory concentration (MIC) for Escherichia coli and Staphylococcus aureus, and its use on in vivo skin lesions. Wistar rats were divided according to treatment: untreated, CM (cellulose membrane), and AgCM (CM incorporated with silver nanoparticles). The euthanasia was performed on the 2nd, 7th, 14th, and 21st days to assess inflammation (myeloperoxidase-neutrophils, N-acetylglucosaminidase-macrophage, IL-1β, IL-10), oxidative stress (NO-nitric oxide, DCF-H2O2), oxidative damage (carbonyl: membrane's damage; sulfhydryl: membrane's integrity), antioxidants (superoxide dismutase; glutathione), angiogenesis, tissue formation (collagen, TGF-β1, smooth muscle α-actin, small decorin, and biglycan proteoglycans). The use of AgCM did not show toxicity, but antibacterial effect in vitro. Moreover, in vivo, AgCM provided balanced oxidative action, modulated the inflammatory profile due to the reduction of IL-1β level and increase in IL-10 level, in addition to increased angiogenesis and collagen formation. The results suggest the use of silver nanoparticles (AgCM) enhanced the CM properties by providing antibacterial properties, modulation the inflammatory phase, and consequently promotes the healing of skin lesions, which can be used clinically to treat injuries.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T12:54:52Z
2023-07-29T12:54:52Z
2023-04-30
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1016/j.bbrc.2023.02.058
Biochemical and Biophysical Research Communications, v. 654, p. 47-54.
1090-2104
0006-291X
http://hdl.handle.net/11449/246947
10.1016/j.bbrc.2023.02.058
2-s2.0-85149468065
url http://dx.doi.org/10.1016/j.bbrc.2023.02.058
http://hdl.handle.net/11449/246947
identifier_str_mv Biochemical and Biophysical Research Communications, v. 654, p. 47-54.
1090-2104
0006-291X
10.1016/j.bbrc.2023.02.058
2-s2.0-85149468065
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Biochemical and Biophysical Research Communications
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 47-54
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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