Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats

Detalhes bibliográficos
Autor(a) principal: de Faria, Felipe Meira
Data de Publicação: 2012
Outros Autores: Luiz-Ferreira, Anderson, Rabelo Socca, Eduardo Augusto, Alves de Almeida, Ana Cristina, Dunder, Ricardo Jose, Manzo, Luis Paulo, da Silva, Marcelo Aparecido [UNESP], Vilegas, Wagner [UNESP], Rozza, Ariane Leite [UNESP], Pellizzon, Claudia Helena [UNESP], Santos, Lourdes Campaner dos [UNESP], Monteiro Souza Brito, Alba Regina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1155/2012/753971
http://hdl.handle.net/11449/18743
Resumo: Male Unib-WH rats were pretreated for two weeks with butanolic (BuOH) and ethyl acetate (EtOAc) fractions. Colitis was induced by rectal administration of TNBS, the treatment continued, and animals were sacrificed on day 7 after the TNBS administration. Phytochemical studies were performed in order to provide the characterization of the tannins present in the bark of R. mangle. Results showed that EtOAc fraction increased the levels of IL-10 (** P < 0.01) and diminished the levels of TNF-alpha (*** P < 0.001) and IL-6 (** P < 0.01). BuOH fraction reduced the MPO activity (** P < 0.01) and levels of TBARS (*** P < 0.001); it also increased COX-1 expression, diminished the levels of TNF-alpha (*** P < 0.001), and increased the levels of IL-12 (*** P < 0.001). Besides, both treatments augmented the levels of GSH (* P < 0.05), the activity of GSH-Px (** P < 0.01 for BuOH fraction and *** P < 0.001 for EtOAc fraction), and CAT (** P < 0.01). In conclusion, both treatments ameliorated the injury induced by TNBS through different mechanisms, probably by their chemical composition which directed its activity into an antioxidant or anti-inflammatory response, leading to an immune modulation.
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spelling Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in RatsMale Unib-WH rats were pretreated for two weeks with butanolic (BuOH) and ethyl acetate (EtOAc) fractions. Colitis was induced by rectal administration of TNBS, the treatment continued, and animals were sacrificed on day 7 after the TNBS administration. Phytochemical studies were performed in order to provide the characterization of the tannins present in the bark of R. mangle. Results showed that EtOAc fraction increased the levels of IL-10 (** P < 0.01) and diminished the levels of TNF-alpha (*** P < 0.001) and IL-6 (** P < 0.01). BuOH fraction reduced the MPO activity (** P < 0.01) and levels of TBARS (*** P < 0.001); it also increased COX-1 expression, diminished the levels of TNF-alpha (*** P < 0.001), and increased the levels of IL-12 (*** P < 0.001). Besides, both treatments augmented the levels of GSH (* P < 0.05), the activity of GSH-Px (** P < 0.01 for BuOH fraction and *** P < 0.001 for EtOAc fraction), and CAT (** P < 0.01). In conclusion, both treatments ameliorated the injury induced by TNBS through different mechanisms, probably by their chemical composition which directed its activity into an antioxidant or anti-inflammatory response, leading to an immune modulation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Campinas, Fac Ciencias Med, Dept Farmacol, UNICAMP, BR-13083970 Campinas, SP, BrazilUniv Estadual Campinas, Dept Biol Estrutural & Func, Inst Biol, BR-13083970 Campinas, SP, BrazilUNESP, Inst Quim, Dept Quim Organ, BR-14800900 Araraquara, SP, BrazilUNESP, Inst Biociencias, Dept Morfol, BR-18618970 Botucatu, SP, BrazilUNESP, Inst Quim, Dept Quim Organ, BR-14800900 Araraquara, SP, BrazilUNESP, Inst Biociencias, Dept Morfol, BR-18618970 Botucatu, SP, BrazilHindawi Publishing CorporationUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)de Faria, Felipe MeiraLuiz-Ferreira, AndersonRabelo Socca, Eduardo AugustoAlves de Almeida, Ana CristinaDunder, Ricardo JoseManzo, Luis Pauloda Silva, Marcelo Aparecido [UNESP]Vilegas, Wagner [UNESP]Rozza, Ariane Leite [UNESP]Pellizzon, Claudia Helena [UNESP]Santos, Lourdes Campaner dos [UNESP]Monteiro Souza Brito, Alba Regina2014-05-20T13:52:28Z2014-05-20T13:52:28Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://dx.doi.org/10.1155/2012/753971Evidence-based Complementary and Alternative Medicine. New York: Hindawi Publishing Corporation, p. 11, 2012.1741-427Xhttp://hdl.handle.net/11449/1874310.1155/2012/753971WOS:000309542000001WOS000309542000001.pdf001939377980106935382536406029770000-0003-3032-25560000-0002-4494-4180Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEvidence-based Complementary and Alternative Medicine2.064info:eu-repo/semantics/openAccess2024-01-03T06:27:24Zoai:repositorio.unesp.br:11449/18743Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:04:06.728869Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats
title Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats
spellingShingle Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats
de Faria, Felipe Meira
title_short Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats
title_full Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats
title_fullStr Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats
title_full_unstemmed Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats
title_sort Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats
author de Faria, Felipe Meira
author_facet de Faria, Felipe Meira
Luiz-Ferreira, Anderson
Rabelo Socca, Eduardo Augusto
Alves de Almeida, Ana Cristina
Dunder, Ricardo Jose
Manzo, Luis Paulo
da Silva, Marcelo Aparecido [UNESP]
Vilegas, Wagner [UNESP]
Rozza, Ariane Leite [UNESP]
Pellizzon, Claudia Helena [UNESP]
Santos, Lourdes Campaner dos [UNESP]
Monteiro Souza Brito, Alba Regina
author_role author
author2 Luiz-Ferreira, Anderson
Rabelo Socca, Eduardo Augusto
Alves de Almeida, Ana Cristina
Dunder, Ricardo Jose
Manzo, Luis Paulo
da Silva, Marcelo Aparecido [UNESP]
Vilegas, Wagner [UNESP]
Rozza, Ariane Leite [UNESP]
Pellizzon, Claudia Helena [UNESP]
Santos, Lourdes Campaner dos [UNESP]
Monteiro Souza Brito, Alba Regina
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual de Campinas (UNICAMP)
Universidade Estadual Paulista (Unesp)
dc.contributor.author.fl_str_mv de Faria, Felipe Meira
Luiz-Ferreira, Anderson
Rabelo Socca, Eduardo Augusto
Alves de Almeida, Ana Cristina
Dunder, Ricardo Jose
Manzo, Luis Paulo
da Silva, Marcelo Aparecido [UNESP]
Vilegas, Wagner [UNESP]
Rozza, Ariane Leite [UNESP]
Pellizzon, Claudia Helena [UNESP]
Santos, Lourdes Campaner dos [UNESP]
Monteiro Souza Brito, Alba Regina
description Male Unib-WH rats were pretreated for two weeks with butanolic (BuOH) and ethyl acetate (EtOAc) fractions. Colitis was induced by rectal administration of TNBS, the treatment continued, and animals were sacrificed on day 7 after the TNBS administration. Phytochemical studies were performed in order to provide the characterization of the tannins present in the bark of R. mangle. Results showed that EtOAc fraction increased the levels of IL-10 (** P < 0.01) and diminished the levels of TNF-alpha (*** P < 0.001) and IL-6 (** P < 0.01). BuOH fraction reduced the MPO activity (** P < 0.01) and levels of TBARS (*** P < 0.001); it also increased COX-1 expression, diminished the levels of TNF-alpha (*** P < 0.001), and increased the levels of IL-12 (*** P < 0.001). Besides, both treatments augmented the levels of GSH (* P < 0.05), the activity of GSH-Px (** P < 0.01 for BuOH fraction and *** P < 0.001 for EtOAc fraction), and CAT (** P < 0.01). In conclusion, both treatments ameliorated the injury induced by TNBS through different mechanisms, probably by their chemical composition which directed its activity into an antioxidant or anti-inflammatory response, leading to an immune modulation.
publishDate 2012
dc.date.none.fl_str_mv 2012-01-01
2014-05-20T13:52:28Z
2014-05-20T13:52:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1155/2012/753971
Evidence-based Complementary and Alternative Medicine. New York: Hindawi Publishing Corporation, p. 11, 2012.
1741-427X
http://hdl.handle.net/11449/18743
10.1155/2012/753971
WOS:000309542000001
WOS000309542000001.pdf
0019393779801069
3538253640602977
0000-0003-3032-2556
0000-0002-4494-4180
url http://dx.doi.org/10.1155/2012/753971
http://hdl.handle.net/11449/18743
identifier_str_mv Evidence-based Complementary and Alternative Medicine. New York: Hindawi Publishing Corporation, p. 11, 2012.
1741-427X
10.1155/2012/753971
WOS:000309542000001
WOS000309542000001.pdf
0019393779801069
3538253640602977
0000-0003-3032-2556
0000-0002-4494-4180
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Evidence-based Complementary and Alternative Medicine
2.064
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11
application/pdf
dc.publisher.none.fl_str_mv Hindawi Publishing Corporation
publisher.none.fl_str_mv Hindawi Publishing Corporation
dc.source.none.fl_str_mv Web of Science
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
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