Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2012/753971 http://hdl.handle.net/11449/18743 |
Resumo: | Male Unib-WH rats were pretreated for two weeks with butanolic (BuOH) and ethyl acetate (EtOAc) fractions. Colitis was induced by rectal administration of TNBS, the treatment continued, and animals were sacrificed on day 7 after the TNBS administration. Phytochemical studies were performed in order to provide the characterization of the tannins present in the bark of R. mangle. Results showed that EtOAc fraction increased the levels of IL-10 (** P < 0.01) and diminished the levels of TNF-alpha (*** P < 0.001) and IL-6 (** P < 0.01). BuOH fraction reduced the MPO activity (** P < 0.01) and levels of TBARS (*** P < 0.001); it also increased COX-1 expression, diminished the levels of TNF-alpha (*** P < 0.001), and increased the levels of IL-12 (*** P < 0.001). Besides, both treatments augmented the levels of GSH (* P < 0.05), the activity of GSH-Px (** P < 0.01 for BuOH fraction and *** P < 0.001 for EtOAc fraction), and CAT (** P < 0.01). In conclusion, both treatments ameliorated the injury induced by TNBS through different mechanisms, probably by their chemical composition which directed its activity into an antioxidant or anti-inflammatory response, leading to an immune modulation. |
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Repositório Institucional da UNESP |
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Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in RatsMale Unib-WH rats were pretreated for two weeks with butanolic (BuOH) and ethyl acetate (EtOAc) fractions. Colitis was induced by rectal administration of TNBS, the treatment continued, and animals were sacrificed on day 7 after the TNBS administration. Phytochemical studies were performed in order to provide the characterization of the tannins present in the bark of R. mangle. Results showed that EtOAc fraction increased the levels of IL-10 (** P < 0.01) and diminished the levels of TNF-alpha (*** P < 0.001) and IL-6 (** P < 0.01). BuOH fraction reduced the MPO activity (** P < 0.01) and levels of TBARS (*** P < 0.001); it also increased COX-1 expression, diminished the levels of TNF-alpha (*** P < 0.001), and increased the levels of IL-12 (*** P < 0.001). Besides, both treatments augmented the levels of GSH (* P < 0.05), the activity of GSH-Px (** P < 0.01 for BuOH fraction and *** P < 0.001 for EtOAc fraction), and CAT (** P < 0.01). In conclusion, both treatments ameliorated the injury induced by TNBS through different mechanisms, probably by their chemical composition which directed its activity into an antioxidant or anti-inflammatory response, leading to an immune modulation.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Univ Estadual Campinas, Fac Ciencias Med, Dept Farmacol, UNICAMP, BR-13083970 Campinas, SP, BrazilUniv Estadual Campinas, Dept Biol Estrutural & Func, Inst Biol, BR-13083970 Campinas, SP, BrazilUNESP, Inst Quim, Dept Quim Organ, BR-14800900 Araraquara, SP, BrazilUNESP, Inst Biociencias, Dept Morfol, BR-18618970 Botucatu, SP, BrazilUNESP, Inst Quim, Dept Quim Organ, BR-14800900 Araraquara, SP, BrazilUNESP, Inst Biociencias, Dept Morfol, BR-18618970 Botucatu, SP, BrazilHindawi Publishing CorporationUniversidade Estadual de Campinas (UNICAMP)Universidade Estadual Paulista (Unesp)de Faria, Felipe MeiraLuiz-Ferreira, AndersonRabelo Socca, Eduardo AugustoAlves de Almeida, Ana CristinaDunder, Ricardo JoseManzo, Luis Pauloda Silva, Marcelo Aparecido [UNESP]Vilegas, Wagner [UNESP]Rozza, Ariane Leite [UNESP]Pellizzon, Claudia Helena [UNESP]Santos, Lourdes Campaner dos [UNESP]Monteiro Souza Brito, Alba Regina2014-05-20T13:52:28Z2014-05-20T13:52:28Z2012-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article11application/pdfhttp://dx.doi.org/10.1155/2012/753971Evidence-based Complementary and Alternative Medicine. New York: Hindawi Publishing Corporation, p. 11, 2012.1741-427Xhttp://hdl.handle.net/11449/1874310.1155/2012/753971WOS:000309542000001WOS000309542000001.pdf001939377980106935382536406029770000-0003-3032-25560000-0002-4494-4180Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengEvidence-based Complementary and Alternative Medicine2.064info:eu-repo/semantics/openAccess2024-01-03T06:27:24Zoai:repositorio.unesp.br:11449/18743Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T22:04:06.728869Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats |
title |
Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats |
spellingShingle |
Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats de Faria, Felipe Meira |
title_short |
Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats |
title_full |
Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats |
title_fullStr |
Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats |
title_full_unstemmed |
Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats |
title_sort |
Effects of Rhizophora mangle on Experimental Colitis Induced by TNBS in Rats |
author |
de Faria, Felipe Meira |
author_facet |
de Faria, Felipe Meira Luiz-Ferreira, Anderson Rabelo Socca, Eduardo Augusto Alves de Almeida, Ana Cristina Dunder, Ricardo Jose Manzo, Luis Paulo da Silva, Marcelo Aparecido [UNESP] Vilegas, Wagner [UNESP] Rozza, Ariane Leite [UNESP] Pellizzon, Claudia Helena [UNESP] Santos, Lourdes Campaner dos [UNESP] Monteiro Souza Brito, Alba Regina |
author_role |
author |
author2 |
Luiz-Ferreira, Anderson Rabelo Socca, Eduardo Augusto Alves de Almeida, Ana Cristina Dunder, Ricardo Jose Manzo, Luis Paulo da Silva, Marcelo Aparecido [UNESP] Vilegas, Wagner [UNESP] Rozza, Ariane Leite [UNESP] Pellizzon, Claudia Helena [UNESP] Santos, Lourdes Campaner dos [UNESP] Monteiro Souza Brito, Alba Regina |
author2_role |
author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual de Campinas (UNICAMP) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
de Faria, Felipe Meira Luiz-Ferreira, Anderson Rabelo Socca, Eduardo Augusto Alves de Almeida, Ana Cristina Dunder, Ricardo Jose Manzo, Luis Paulo da Silva, Marcelo Aparecido [UNESP] Vilegas, Wagner [UNESP] Rozza, Ariane Leite [UNESP] Pellizzon, Claudia Helena [UNESP] Santos, Lourdes Campaner dos [UNESP] Monteiro Souza Brito, Alba Regina |
description |
Male Unib-WH rats were pretreated for two weeks with butanolic (BuOH) and ethyl acetate (EtOAc) fractions. Colitis was induced by rectal administration of TNBS, the treatment continued, and animals were sacrificed on day 7 after the TNBS administration. Phytochemical studies were performed in order to provide the characterization of the tannins present in the bark of R. mangle. Results showed that EtOAc fraction increased the levels of IL-10 (** P < 0.01) and diminished the levels of TNF-alpha (*** P < 0.001) and IL-6 (** P < 0.01). BuOH fraction reduced the MPO activity (** P < 0.01) and levels of TBARS (*** P < 0.001); it also increased COX-1 expression, diminished the levels of TNF-alpha (*** P < 0.001), and increased the levels of IL-12 (*** P < 0.001). Besides, both treatments augmented the levels of GSH (* P < 0.05), the activity of GSH-Px (** P < 0.01 for BuOH fraction and *** P < 0.001 for EtOAc fraction), and CAT (** P < 0.01). In conclusion, both treatments ameliorated the injury induced by TNBS through different mechanisms, probably by their chemical composition which directed its activity into an antioxidant or anti-inflammatory response, leading to an immune modulation. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-01-01 2014-05-20T13:52:28Z 2014-05-20T13:52:28Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2012/753971 Evidence-based Complementary and Alternative Medicine. New York: Hindawi Publishing Corporation, p. 11, 2012. 1741-427X http://hdl.handle.net/11449/18743 10.1155/2012/753971 WOS:000309542000001 WOS000309542000001.pdf 0019393779801069 3538253640602977 0000-0003-3032-2556 0000-0002-4494-4180 |
url |
http://dx.doi.org/10.1155/2012/753971 http://hdl.handle.net/11449/18743 |
identifier_str_mv |
Evidence-based Complementary and Alternative Medicine. New York: Hindawi Publishing Corporation, p. 11, 2012. 1741-427X 10.1155/2012/753971 WOS:000309542000001 WOS000309542000001.pdf 0019393779801069 3538253640602977 0000-0003-3032-2556 0000-0002-4494-4180 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Evidence-based Complementary and Alternative Medicine 2.064 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
11 application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808129388645974016 |