The influence of hydration on the architectural rearrangement of normal and neoplastic human breast tissues
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.heliyon.2019.e01219 http://hdl.handle.net/11449/187366 |
Resumo: | In adult women, the water-content represents between 50% and 70% of the mass in normal breast tissues and this percentage is increased within diseased tissues. Water molecules play an essential role in the structural organization of biological tissues such as breast. Then, in this study, we have investigated the influence of the water molecules on the breast tissue organization and their role on the hierarchical tissue rearrangement promoted by tumor growth. SAXS and WAXS techniques were used to analyze healthy, benign and malignant human breast samples in native and lyophilized conditions. The scattering profiles in SAXS and WAXS regime of each tissue type in both conditions were compared in order to identify the structural transformation in these tissues and verify the water influence on the morphological arrangement of normal and pathological human breast tissues. From SAXS, changes at the axial periodicity of collagen fibrils were revealed. Additionally, when the water content has removed a peak at q = 4.17 nm −1 (that was present only in pathological samples) shifted in opposite directions within benign and malignant lesions. From WAXS, water and fatty acids were identified within native samples. However, after freeze-drying, only the fat component was observed in the scattering profiles. Therefore, when the water molecules were removed from the samples, structural changes associated with pathological progression were visible. From this, insights about their influence over the changes promoted by the tumor growth have been proposed. Finally, the findings of this study have the potential to provide valuable information to the development of new target therapy. |
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The influence of hydration on the architectural rearrangement of normal and neoplastic human breast tissuesBiomedical engineeringCancer researchMolecular physicsStructural biologyIn adult women, the water-content represents between 50% and 70% of the mass in normal breast tissues and this percentage is increased within diseased tissues. Water molecules play an essential role in the structural organization of biological tissues such as breast. Then, in this study, we have investigated the influence of the water molecules on the breast tissue organization and their role on the hierarchical tissue rearrangement promoted by tumor growth. SAXS and WAXS techniques were used to analyze healthy, benign and malignant human breast samples in native and lyophilized conditions. The scattering profiles in SAXS and WAXS regime of each tissue type in both conditions were compared in order to identify the structural transformation in these tissues and verify the water influence on the morphological arrangement of normal and pathological human breast tissues. From SAXS, changes at the axial periodicity of collagen fibrils were revealed. Additionally, when the water content has removed a peak at q = 4.17 nm −1 (that was present only in pathological samples) shifted in opposite directions within benign and malignant lesions. From WAXS, water and fatty acids were identified within native samples. However, after freeze-drying, only the fat component was observed in the scattering profiles. Therefore, when the water molecules were removed from the samples, structural changes associated with pathological progression were visible. From this, insights about their influence over the changes promoted by the tumor growth have been proposed. Finally, the findings of this study have the potential to provide valuable information to the development of new target therapy.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Tecnológica Federal do Paraná, Av. Sete de Setembro 3165Deutsches Elektronen-Synchrotron DESY, Notkestraße 85School of Biomedical Sciences and Laboratory Medicine The University of Western Australia, 35 Stirling HighwayDepartamento de Física Faculdade de Ciências UNESP, Av. Luiz Edmundo Carrijo Coube 14-01Departamento de Física FFCLRP Universidade de São Paulo, Ribeirão PretoDepartamento de Física Faculdade de Ciências UNESP, Av. Luiz Edmundo Carrijo Coube 14-01FAPESP: 2011/20632-6CNPq: D02A-SAXS2Universidade Tecnológica Federal do ParanáDeutsches Elektronen-Synchrotron DESYThe University of Western AustraliaUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Conceição, A. L.C.Meehan, K.Antoniassi, M.Piacenti-Silva, M. [UNESP]Poletti, M. E.2019-10-06T15:33:58Z2019-10-06T15:33:58Z2019-02-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.heliyon.2019.e01219Heliyon, v. 5, n. 2, 2019.2405-8440http://hdl.handle.net/11449/18736610.1016/j.heliyon.2019.e012192-s2.0-85061674218Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHeliyoninfo:eu-repo/semantics/openAccess2021-10-23T19:23:37Zoai:repositorio.unesp.br:11449/187366Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:57:00.571344Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The influence of hydration on the architectural rearrangement of normal and neoplastic human breast tissues |
title |
The influence of hydration on the architectural rearrangement of normal and neoplastic human breast tissues |
spellingShingle |
The influence of hydration on the architectural rearrangement of normal and neoplastic human breast tissues Conceição, A. L.C. Biomedical engineering Cancer research Molecular physics Structural biology |
title_short |
The influence of hydration on the architectural rearrangement of normal and neoplastic human breast tissues |
title_full |
The influence of hydration on the architectural rearrangement of normal and neoplastic human breast tissues |
title_fullStr |
The influence of hydration on the architectural rearrangement of normal and neoplastic human breast tissues |
title_full_unstemmed |
The influence of hydration on the architectural rearrangement of normal and neoplastic human breast tissues |
title_sort |
The influence of hydration on the architectural rearrangement of normal and neoplastic human breast tissues |
author |
Conceição, A. L.C. |
author_facet |
Conceição, A. L.C. Meehan, K. Antoniassi, M. Piacenti-Silva, M. [UNESP] Poletti, M. E. |
author_role |
author |
author2 |
Meehan, K. Antoniassi, M. Piacenti-Silva, M. [UNESP] Poletti, M. E. |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Tecnológica Federal do Paraná Deutsches Elektronen-Synchrotron DESY The University of Western Australia Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Conceição, A. L.C. Meehan, K. Antoniassi, M. Piacenti-Silva, M. [UNESP] Poletti, M. E. |
dc.subject.por.fl_str_mv |
Biomedical engineering Cancer research Molecular physics Structural biology |
topic |
Biomedical engineering Cancer research Molecular physics Structural biology |
description |
In adult women, the water-content represents between 50% and 70% of the mass in normal breast tissues and this percentage is increased within diseased tissues. Water molecules play an essential role in the structural organization of biological tissues such as breast. Then, in this study, we have investigated the influence of the water molecules on the breast tissue organization and their role on the hierarchical tissue rearrangement promoted by tumor growth. SAXS and WAXS techniques were used to analyze healthy, benign and malignant human breast samples in native and lyophilized conditions. The scattering profiles in SAXS and WAXS regime of each tissue type in both conditions were compared in order to identify the structural transformation in these tissues and verify the water influence on the morphological arrangement of normal and pathological human breast tissues. From SAXS, changes at the axial periodicity of collagen fibrils were revealed. Additionally, when the water content has removed a peak at q = 4.17 nm −1 (that was present only in pathological samples) shifted in opposite directions within benign and malignant lesions. From WAXS, water and fatty acids were identified within native samples. However, after freeze-drying, only the fat component was observed in the scattering profiles. Therefore, when the water molecules were removed from the samples, structural changes associated with pathological progression were visible. From this, insights about their influence over the changes promoted by the tumor growth have been proposed. Finally, the findings of this study have the potential to provide valuable information to the development of new target therapy. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:33:58Z 2019-10-06T15:33:58Z 2019-02-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.heliyon.2019.e01219 Heliyon, v. 5, n. 2, 2019. 2405-8440 http://hdl.handle.net/11449/187366 10.1016/j.heliyon.2019.e01219 2-s2.0-85061674218 |
url |
http://dx.doi.org/10.1016/j.heliyon.2019.e01219 http://hdl.handle.net/11449/187366 |
identifier_str_mv |
Heliyon, v. 5, n. 2, 2019. 2405-8440 10.1016/j.heliyon.2019.e01219 2-s2.0-85061674218 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Heliyon |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129002881155072 |