Autophagy is associated with male sterility in pistillate flowers of Maytenus obtusifolia (Celastraceae)
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1071/BT17174 http://hdl.handle.net/11449/164097 |
Resumo: | Programmed cell death (PCD) is defined as a sequence of genetically regulated events leading to controlled and organised cellular degradation. It plays a vital role in plant development; however, little is known about the role of PCD in reproductive development. Sterility in pistillate flowers of Maytenus obtusifolia Mart. has been shown to be related to cytoplasmic male sterility (CMS) based on reproductive biology and anatomical analysis. The recurrent PCD led us to investigate changes in the tapetum and sporogenic tissue during the establishment of male sterility using light and transmission electron microscopy combined with the use of TUNEL (terminal deoxynucleotidyl transferase mediated dUDP end-labelling) assay. The interruption of pollen development in pistillate flowers is a result of premature PCD in the tapetum and consequently in the sporogenic cells. Autophagy, via macroautophagy, occurs in the sporogenic cells and involves the formation of autophagosomes, through rough endoplasmic reticulum, and of complex macroautophagic structures. In the final stage of PCD, massive autophagy takes place. Male sterility in female individuals is thus reasonably interpreted as sporophytic CMS associated to autophagy. |
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Repositório Institucional da UNESP |
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Autophagy is associated with male sterility in pistillate flowers of Maytenus obtusifolia (Celastraceae)autophagycytochemistryflowerPCDsterilityTUNELultrastructurevacuolar cell deathProgrammed cell death (PCD) is defined as a sequence of genetically regulated events leading to controlled and organised cellular degradation. It plays a vital role in plant development; however, little is known about the role of PCD in reproductive development. Sterility in pistillate flowers of Maytenus obtusifolia Mart. has been shown to be related to cytoplasmic male sterility (CMS) based on reproductive biology and anatomical analysis. The recurrent PCD led us to investigate changes in the tapetum and sporogenic tissue during the establishment of male sterility using light and transmission electron microscopy combined with the use of TUNEL (terminal deoxynucleotidyl transferase mediated dUDP end-labelling) assay. The interruption of pollen development in pistillate flowers is a result of premature PCD in the tapetum and consequently in the sporogenic cells. Autophagy, via macroautophagy, occurs in the sporogenic cells and involves the formation of autophagosomes, through rough endoplasmic reticulum, and of complex macroautophagic structures. In the final stage of PCD, massive autophagy takes place. Male sterility in female individuals is thus reasonably interpreted as sporophytic CMS associated to autophagy.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Univ Fed Rio de Janeiro, Dept Bot, Museu Nacl, BR-22940040 Rio De Janeiro, RJ, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Bot, Caixa Postal 510, BR-18618000 Botucatu, SP, BrazilUniv Estadual Paulista, Inst Biociencias, Dept Bot, Caixa Postal 510, BR-18618000 Botucatu, SP, BrazilCNPq: 473289/2010CNPq: 447624/2014-8CNPq: 302657/2011-8Csiro PublishingUniversidade Federal do Rio de Janeiro (UFRJ)Universidade Estadual Paulista (Unesp)Nader Haddad, Isabella VerissimoRibeiro de Santiago-Fernandes, Lygia DoloresMachado, Silvia Rodrigues [UNESP]2018-11-26T17:49:06Z2018-11-26T17:49:06Z2018-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article108-115http://dx.doi.org/10.1071/BT17174Australian Journal Of Botany. Clayton: Csiro Publishing, v. 66, n. 2, p. 108-115, 2018.0067-1924http://hdl.handle.net/11449/16409710.1071/BT17174WOS:000429735400003Web of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAustralian Journal Of Botany0,393info:eu-repo/semantics/openAccess2021-10-23T15:09:04Zoai:repositorio.unesp.br:11449/164097Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T20:27:10.489969Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Autophagy is associated with male sterility in pistillate flowers of Maytenus obtusifolia (Celastraceae) |
title |
Autophagy is associated with male sterility in pistillate flowers of Maytenus obtusifolia (Celastraceae) |
spellingShingle |
Autophagy is associated with male sterility in pistillate flowers of Maytenus obtusifolia (Celastraceae) Nader Haddad, Isabella Verissimo autophagy cytochemistry flower PCD sterility TUNEL ultrastructure vacuolar cell death |
title_short |
Autophagy is associated with male sterility in pistillate flowers of Maytenus obtusifolia (Celastraceae) |
title_full |
Autophagy is associated with male sterility in pistillate flowers of Maytenus obtusifolia (Celastraceae) |
title_fullStr |
Autophagy is associated with male sterility in pistillate flowers of Maytenus obtusifolia (Celastraceae) |
title_full_unstemmed |
Autophagy is associated with male sterility in pistillate flowers of Maytenus obtusifolia (Celastraceae) |
title_sort |
Autophagy is associated with male sterility in pistillate flowers of Maytenus obtusifolia (Celastraceae) |
author |
Nader Haddad, Isabella Verissimo |
author_facet |
Nader Haddad, Isabella Verissimo Ribeiro de Santiago-Fernandes, Lygia Dolores Machado, Silvia Rodrigues [UNESP] |
author_role |
author |
author2 |
Ribeiro de Santiago-Fernandes, Lygia Dolores Machado, Silvia Rodrigues [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Federal do Rio de Janeiro (UFRJ) Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Nader Haddad, Isabella Verissimo Ribeiro de Santiago-Fernandes, Lygia Dolores Machado, Silvia Rodrigues [UNESP] |
dc.subject.por.fl_str_mv |
autophagy cytochemistry flower PCD sterility TUNEL ultrastructure vacuolar cell death |
topic |
autophagy cytochemistry flower PCD sterility TUNEL ultrastructure vacuolar cell death |
description |
Programmed cell death (PCD) is defined as a sequence of genetically regulated events leading to controlled and organised cellular degradation. It plays a vital role in plant development; however, little is known about the role of PCD in reproductive development. Sterility in pistillate flowers of Maytenus obtusifolia Mart. has been shown to be related to cytoplasmic male sterility (CMS) based on reproductive biology and anatomical analysis. The recurrent PCD led us to investigate changes in the tapetum and sporogenic tissue during the establishment of male sterility using light and transmission electron microscopy combined with the use of TUNEL (terminal deoxynucleotidyl transferase mediated dUDP end-labelling) assay. The interruption of pollen development in pistillate flowers is a result of premature PCD in the tapetum and consequently in the sporogenic cells. Autophagy, via macroautophagy, occurs in the sporogenic cells and involves the formation of autophagosomes, through rough endoplasmic reticulum, and of complex macroautophagic structures. In the final stage of PCD, massive autophagy takes place. Male sterility in female individuals is thus reasonably interpreted as sporophytic CMS associated to autophagy. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-26T17:49:06Z 2018-11-26T17:49:06Z 2018-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1071/BT17174 Australian Journal Of Botany. Clayton: Csiro Publishing, v. 66, n. 2, p. 108-115, 2018. 0067-1924 http://hdl.handle.net/11449/164097 10.1071/BT17174 WOS:000429735400003 |
url |
http://dx.doi.org/10.1071/BT17174 http://hdl.handle.net/11449/164097 |
identifier_str_mv |
Australian Journal Of Botany. Clayton: Csiro Publishing, v. 66, n. 2, p. 108-115, 2018. 0067-1924 10.1071/BT17174 WOS:000429735400003 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Australian Journal Of Botany 0,393 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
108-115 |
dc.publisher.none.fl_str_mv |
Csiro Publishing |
publisher.none.fl_str_mv |
Csiro Publishing |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129203878494208 |