Development of microsatellite loci for Cryptocarya mandioccana Meisner (Lauraceae) and their genotyping success in different tissues
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/2236-8906-22/2017 http://hdl.handle.net/11449/212227 |
Resumo: | Nine polymorphic microsatellite loci were isolated and characterized for Cryptocarya mandioccana Meisner, a tree from the Atlantic Rainforest with seeds dispersed by large animals. The loci were characterized using 48 individuals from two populations and their genotyping success tested in four tissues: leaves from adults and seedlings, and two diaspore maternal tissues. Maternity analyses were also performed on diaspores and leaves from nine adult trees. The number of alleles per locus ranged from nine to 15 and the observed and expected heterozygosities ranged from 0.214 to 0.864 and 0.745 to 0.892, respectively. The loci genotyping success did not significantly differ between tissues and varied from 56 to 96%. The microsatellites showed enough polymorphism to assign the nine adult trees to their diaspores. The successful genotyping in all tissues and identification of mother trees show that the microsatellites are suitable for studies such as spatial genetic structure and maternity analyses. |
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Development of microsatellite loci for Cryptocarya mandioccana Meisner (Lauraceae) and their genotyping success in different tissuesDesenvolvimento de locos de microssatélites para Cryptocarya mandioccana Meisner (Lauraceae) e o sucesso de genotipagem em diferentes tecidosAtlantic forestLauraceaematernity analysismicrosatelliteseed maternal tissueanálise de maternidadeLauraceaeMata Atlânticamicrossatélitetecido materno das sementesNine polymorphic microsatellite loci were isolated and characterized for Cryptocarya mandioccana Meisner, a tree from the Atlantic Rainforest with seeds dispersed by large animals. The loci were characterized using 48 individuals from two populations and their genotyping success tested in four tissues: leaves from adults and seedlings, and two diaspore maternal tissues. Maternity analyses were also performed on diaspores and leaves from nine adult trees. The number of alleles per locus ranged from nine to 15 and the observed and expected heterozygosities ranged from 0.214 to 0.864 and 0.745 to 0.892, respectively. The loci genotyping success did not significantly differ between tissues and varied from 56 to 96%. The microsatellites showed enough polymorphism to assign the nine adult trees to their diaspores. The successful genotyping in all tissues and identification of mother trees show that the microsatellites are suitable for studies such as spatial genetic structure and maternity analyses.Nove locos de microssatélites polimórficos foram isolados e caracterizados para Cryptocarya mandioccana Meisner, uma árvore da Mata Atlântica cujas sementes são dispersas por grandes animais. Os locos foram caracterizados usando 48 indivíduos de duas populações e o sucesso de genotipagem testado em quatro tecidos: folhas de adultos e plântulas, e dois tecidos maternos de diásporos. Análises de maternidade foram também realizadas em diásporos e folhas de nove árvores adultas. O número de alelos por loco variou de nove a 15 e as heterozigosidades observada e esperada de 0,214 a 0,864 e 0,745 a 0,892, respectivamente. O sucesso de genotipagem dos locos não diferiu entre os tecidos e variou de 56 a 96%. Os microssatélites mostraram polimorfismo suficiente para atribuir as nove árvores aos seus diásporos. O sucesso de genotipagem em todos os tecidos e a identificação das árvores mães mostraram que os microssatélites são adequados para estudos como estrutura genética espacial e análise de maternidade.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista Júlio de Mesquita Filho, Instituto de BiociênciasUniversidade Estadual Paulista Júlio de Mesquita Filho, Instituto de BiociênciasFAPESP: 2014/20621-2; 2014/20622-9; 2014/01029-5; 2010/16075-1; 2009/52725-3; 2014/14739-0CAPES: 009/10CNPq: 456549/2014-5Instituto de BotânicaUniversidade Estadual Paulista (Unesp)Carvalho, Thabata [UNESP]Garcia, Giuliana [UNESP]Carvalho, Carolina Silva [UNESP]Palma-silva, Clarisse [UNESP]Culot, Laurence [UNESP]2021-07-14T10:36:37Z2021-07-14T10:36:37Z2017info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article580-587application/pdfhttp://dx.doi.org/10.1590/2236-8906-22/2017Hoehnea. Instituto de Botânica, v. 44, n. 4, p. 580-587, 2017.0073-28772236-8906http://hdl.handle.net/11449/21222710.1590/2236-8906-22/2017S2236-89062017000400580S2236-89062017000400580.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengHoehneainfo:eu-repo/semantics/openAccess2023-11-15T06:18:43Zoai:repositorio.unesp.br:11449/212227Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:47:45.952921Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Development of microsatellite loci for Cryptocarya mandioccana Meisner (Lauraceae) and their genotyping success in different tissues Desenvolvimento de locos de microssatélites para Cryptocarya mandioccana Meisner (Lauraceae) e o sucesso de genotipagem em diferentes tecidos |
title |
Development of microsatellite loci for Cryptocarya mandioccana Meisner (Lauraceae) and their genotyping success in different tissues |
spellingShingle |
Development of microsatellite loci for Cryptocarya mandioccana Meisner (Lauraceae) and their genotyping success in different tissues Carvalho, Thabata [UNESP] Atlantic forest Lauraceae maternity analysis microsatellite seed maternal tissue análise de maternidade Lauraceae Mata Atlântica microssatélite tecido materno das sementes |
title_short |
Development of microsatellite loci for Cryptocarya mandioccana Meisner (Lauraceae) and their genotyping success in different tissues |
title_full |
Development of microsatellite loci for Cryptocarya mandioccana Meisner (Lauraceae) and their genotyping success in different tissues |
title_fullStr |
Development of microsatellite loci for Cryptocarya mandioccana Meisner (Lauraceae) and their genotyping success in different tissues |
title_full_unstemmed |
Development of microsatellite loci for Cryptocarya mandioccana Meisner (Lauraceae) and their genotyping success in different tissues |
title_sort |
Development of microsatellite loci for Cryptocarya mandioccana Meisner (Lauraceae) and their genotyping success in different tissues |
author |
Carvalho, Thabata [UNESP] |
author_facet |
Carvalho, Thabata [UNESP] Garcia, Giuliana [UNESP] Carvalho, Carolina Silva [UNESP] Palma-silva, Clarisse [UNESP] Culot, Laurence [UNESP] |
author_role |
author |
author2 |
Garcia, Giuliana [UNESP] Carvalho, Carolina Silva [UNESP] Palma-silva, Clarisse [UNESP] Culot, Laurence [UNESP] |
author2_role |
author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Carvalho, Thabata [UNESP] Garcia, Giuliana [UNESP] Carvalho, Carolina Silva [UNESP] Palma-silva, Clarisse [UNESP] Culot, Laurence [UNESP] |
dc.subject.por.fl_str_mv |
Atlantic forest Lauraceae maternity analysis microsatellite seed maternal tissue análise de maternidade Lauraceae Mata Atlântica microssatélite tecido materno das sementes |
topic |
Atlantic forest Lauraceae maternity analysis microsatellite seed maternal tissue análise de maternidade Lauraceae Mata Atlântica microssatélite tecido materno das sementes |
description |
Nine polymorphic microsatellite loci were isolated and characterized for Cryptocarya mandioccana Meisner, a tree from the Atlantic Rainforest with seeds dispersed by large animals. The loci were characterized using 48 individuals from two populations and their genotyping success tested in four tissues: leaves from adults and seedlings, and two diaspore maternal tissues. Maternity analyses were also performed on diaspores and leaves from nine adult trees. The number of alleles per locus ranged from nine to 15 and the observed and expected heterozygosities ranged from 0.214 to 0.864 and 0.745 to 0.892, respectively. The loci genotyping success did not significantly differ between tissues and varied from 56 to 96%. The microsatellites showed enough polymorphism to assign the nine adult trees to their diaspores. The successful genotyping in all tissues and identification of mother trees show that the microsatellites are suitable for studies such as spatial genetic structure and maternity analyses. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2021-07-14T10:36:37Z 2021-07-14T10:36:37Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/2236-8906-22/2017 Hoehnea. Instituto de Botânica, v. 44, n. 4, p. 580-587, 2017. 0073-2877 2236-8906 http://hdl.handle.net/11449/212227 10.1590/2236-8906-22/2017 S2236-89062017000400580 S2236-89062017000400580.pdf |
url |
http://dx.doi.org/10.1590/2236-8906-22/2017 http://hdl.handle.net/11449/212227 |
identifier_str_mv |
Hoehnea. Instituto de Botânica, v. 44, n. 4, p. 580-587, 2017. 0073-2877 2236-8906 10.1590/2236-8906-22/2017 S2236-89062017000400580 S2236-89062017000400580.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Hoehnea |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
580-587 application/pdf |
dc.publisher.none.fl_str_mv |
Instituto de Botânica |
publisher.none.fl_str_mv |
Instituto de Botânica |
dc.source.none.fl_str_mv |
SciELO reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128859373043712 |