Modulation of cytokines production by indomethacin acute dose during the evolution of ehrlich ascites tumor in mice
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1155/2015/924028 http://hdl.handle.net/11449/131115 |
Resumo: | The aim of the present study was to investigate the influence of a nonselective COX1/COX2 inhibitor (indomethacin) on tumor growth of Ehrlich Ascites Tumor (EAT) in mice, using as parameters the tumor growth and cytokine profile. Mice were inoculated with EAT cells and treated with indomethacin. After 1, 3, 6, 10, and 13 days the animals were evaluated for the secretion of TNFα, IL-1α, IL-2, IL-4, IL-6, IL-10, and IL-13 and PGE2 level in peritoneal cavity. The results have shown that EAT induces PGE2 production and increases tumor cells number from the 10th day. The cytokine profile showed EAT induces production of IL-6 from 10th day and of IL-2 on 13th day; the other studied cytokines were not affected in a significant way. The indomethacin treatment of EAT-bearing mice inhibited the tumor growth and PGE2 synthesis from the 10th day. In addition, the treatment of EAT-bearing mice with indomethacin has stimulated the IL-13 production and has significantly inhibited IL-6 in the 13th day of tumor growth. Taken together, the results have demonstrated that EAT growth is modulated by PGE2 and the inhibition of the tumor growth could be partly related to suppression of IL-6 and induction of IL-13. |
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Repositório Institucional da UNESP |
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Modulation of cytokines production by indomethacin acute dose during the evolution of ehrlich ascites tumor in miceThe aim of the present study was to investigate the influence of a nonselective COX1/COX2 inhibitor (indomethacin) on tumor growth of Ehrlich Ascites Tumor (EAT) in mice, using as parameters the tumor growth and cytokine profile. Mice were inoculated with EAT cells and treated with indomethacin. After 1, 3, 6, 10, and 13 days the animals were evaluated for the secretion of TNFα, IL-1α, IL-2, IL-4, IL-6, IL-10, and IL-13 and PGE2 level in peritoneal cavity. The results have shown that EAT induces PGE2 production and increases tumor cells number from the 10th day. The cytokine profile showed EAT induces production of IL-6 from 10th day and of IL-2 on 13th day; the other studied cytokines were not affected in a significant way. The indomethacin treatment of EAT-bearing mice inhibited the tumor growth and PGE2 synthesis from the 10th day. In addition, the treatment of EAT-bearing mice with indomethacin has stimulated the IL-13 production and has significantly inhibited IL-6 in the 13th day of tumor growth. Taken together, the results have demonstrated that EAT growth is modulated by PGE2 and the inhibition of the tumor growth could be partly related to suppression of IL-6 and induction of IL-13.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Laboratory of Glycobiology, Carlos Chagas Filho Biophysics Institute (IBCCF), Federal University of Rio de Janeiro (UFRJ), 21941-902 Rio de Janeiro, RJ, BrazilApplied Pharmacology and Toxicology Laboratory, School of Veterinary Medicine, University of São Paulo, 05508-900 São Paulo, SP, Brazil.Department of Pathology, School of Medicine, São Paulo State University (UNESP), 18618-970 Botucatu, SP, BrazilFAPESP: 1998/16096-5Hindawi Publishing CorporationUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Universidade Federal do Rio de Janeiro (UFRJ)Gentile, Luciana Boffoni [UNESP]Queiroz-Hazarbassanov, NicolleMassoco, Cristina de OliveiraFecchio, Denise [UNESP]2015-12-07T15:31:43Z2015-12-07T15:31:43Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-8application/pdfhttp://dx.doi.org/10.1155/2015/924028Mediators Of Inflammation, v. 2015, p. 1-8, 2015.1466-1861http://hdl.handle.net/11449/13111510.1155/2015/924028PMC4549603.pdf26347589PMC4549603PubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMediators Of Inflammation1,370info:eu-repo/semantics/openAccess2024-09-03T13:18:03Zoai:repositorio.unesp.br:11449/131115Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestrepositoriounesp@unesp.bropendoar:29462024-09-03T13:18:03Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Modulation of cytokines production by indomethacin acute dose during the evolution of ehrlich ascites tumor in mice |
title |
Modulation of cytokines production by indomethacin acute dose during the evolution of ehrlich ascites tumor in mice |
spellingShingle |
Modulation of cytokines production by indomethacin acute dose during the evolution of ehrlich ascites tumor in mice Gentile, Luciana Boffoni [UNESP] |
title_short |
Modulation of cytokines production by indomethacin acute dose during the evolution of ehrlich ascites tumor in mice |
title_full |
Modulation of cytokines production by indomethacin acute dose during the evolution of ehrlich ascites tumor in mice |
title_fullStr |
Modulation of cytokines production by indomethacin acute dose during the evolution of ehrlich ascites tumor in mice |
title_full_unstemmed |
Modulation of cytokines production by indomethacin acute dose during the evolution of ehrlich ascites tumor in mice |
title_sort |
Modulation of cytokines production by indomethacin acute dose during the evolution of ehrlich ascites tumor in mice |
author |
Gentile, Luciana Boffoni [UNESP] |
author_facet |
Gentile, Luciana Boffoni [UNESP] Queiroz-Hazarbassanov, Nicolle Massoco, Cristina de Oliveira Fecchio, Denise [UNESP] |
author_role |
author |
author2 |
Queiroz-Hazarbassanov, Nicolle Massoco, Cristina de Oliveira Fecchio, Denise [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade de São Paulo (USP) Universidade Federal do Rio de Janeiro (UFRJ) |
dc.contributor.author.fl_str_mv |
Gentile, Luciana Boffoni [UNESP] Queiroz-Hazarbassanov, Nicolle Massoco, Cristina de Oliveira Fecchio, Denise [UNESP] |
description |
The aim of the present study was to investigate the influence of a nonselective COX1/COX2 inhibitor (indomethacin) on tumor growth of Ehrlich Ascites Tumor (EAT) in mice, using as parameters the tumor growth and cytokine profile. Mice were inoculated with EAT cells and treated with indomethacin. After 1, 3, 6, 10, and 13 days the animals were evaluated for the secretion of TNFα, IL-1α, IL-2, IL-4, IL-6, IL-10, and IL-13 and PGE2 level in peritoneal cavity. The results have shown that EAT induces PGE2 production and increases tumor cells number from the 10th day. The cytokine profile showed EAT induces production of IL-6 from 10th day and of IL-2 on 13th day; the other studied cytokines were not affected in a significant way. The indomethacin treatment of EAT-bearing mice inhibited the tumor growth and PGE2 synthesis from the 10th day. In addition, the treatment of EAT-bearing mice with indomethacin has stimulated the IL-13 production and has significantly inhibited IL-6 in the 13th day of tumor growth. Taken together, the results have demonstrated that EAT growth is modulated by PGE2 and the inhibition of the tumor growth could be partly related to suppression of IL-6 and induction of IL-13. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-07T15:31:43Z 2015-12-07T15:31:43Z 2015 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1155/2015/924028 Mediators Of Inflammation, v. 2015, p. 1-8, 2015. 1466-1861 http://hdl.handle.net/11449/131115 10.1155/2015/924028 PMC4549603.pdf 26347589 PMC4549603 |
url |
http://dx.doi.org/10.1155/2015/924028 http://hdl.handle.net/11449/131115 |
identifier_str_mv |
Mediators Of Inflammation, v. 2015, p. 1-8, 2015. 1466-1861 10.1155/2015/924028 PMC4549603.pdf 26347589 PMC4549603 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Mediators Of Inflammation 1,370 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-8 application/pdf |
dc.publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
publisher.none.fl_str_mv |
Hindawi Publishing Corporation |
dc.source.none.fl_str_mv |
PubMed reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
repositoriounesp@unesp.br |
_version_ |
1810021403052736512 |