Análise do potencial angiogênico da terapia fotodinâmica antimicrobiana em ratas tratadas com dose oncológica de Zoledronato

• Autor(a) principal: Rodrigues, João Vicente Feres [UNESP]
• Data de Publicação: 2016
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/155263; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-04-28/000881313.pdf
• topic: Difosfonatos; Osteonecrose; Fotoquimioterapia; Ratos; Arcada osseodentária; Diphosphonates

Bisphosphonates (BPs) are potent drugs, with antiresorptive properties, used for treatment of some bone diseases or orthotropic malignancies. A serious adverse effect caused by the use of BPs is bisphosphonate-related osteonecrosis of the jaw (BRONJ). Vasculature involvement is claimed as one of the possible pathogenetic factors of BRONJ, however, existing studies are inconclusive, inconsistent and contradictory. As the etiology of this pathological condition is poorly understood, there is great difficulty in preventing and successfully treating of this disease. One of the few preventive therapeutic approaches is antibiotic therapy, which has proven to be unsuccessful. Antimicrobial photodynamic therapy (aPDT) has concurrently shown antimicrobial capacity and bioestimulation abilities, for the vasculature as well, which places it as a potential preventive therapeutic strategy for BRONJ. The objectives of this study were as follows: to analyze vasculature involvement on the pathogenesis of BRONJ in rats that showed the main risk factors for developing the disease and evaluate the effect of aPDT on the vasculature when applied to the tooth extraction site. Thirty senile rats (20 months old) were divided into four experimental groups: SAL, ZOL, SAL/aPDT and ZOL/aPDT. Drug treatment plan lasted seven weeks. The rats received intraperitoneal injections of 0.45 ml of 0.9% sodium chloride solution (Groups SAL and SAL/aPDT) or 0.45 ml of the same solution plus 100 ug/kg zoledronate (Groups ZOL and ZOL/aPDT) with an interval of two days between applications. After three weeks of treatment all rats were subjected to dental extraction of the first lower left molar. In groups SAL/aPDT and ZOL/aPDT rats were subjected to aPDT sessions at 0, 2 and 4 days after the tooth extraction. The aPDT consisted of applying of the photosensitizer (500 μl of methylene...