Efeito da terapia fotodinâmica antimicrobiana (aPDT) sobre o processo de reparo alveolar de ratas tratadas com dose oncológica de zoledronato: estudo histológico, histométrico e histoquímico

• Autor(a) principal: Mello Neto, João Martins de [UNESP]
• Data de Publicação: 2015
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://hdl.handle.net/11449/149690; http://www.athena.biblioteca.unesp.br/exlibris/bd/capelo/2017-01-31/000867801.pdf
• topic: Osteonecrose; Arcada osseodentária; Fotoquimioterapia; Osteonecrosis

Bisphosphonates (BPs) are class of drugs used in the treatment of some bone diseases or osteopathies or osteotropic malignancies. Among the adverse effects is the occurrence of medication-related osteonecrosis of the jaws (MRONJ). The pathogenesis is poorly understood, which hinders its prevention and treatment. Among the few therapeutic approaches is preventive antibiotic therapy, which shows failure. ntimicrobial photodynamic therapy (aPDT) displays antimicrobial activity and potent biostimulatory action, which places it as a potential therapeutic strategy for preventive the ONM-BPs. The aim of this study was to evaluate the effect of the aPDT in dental extraction sockets of rats that are the main risk factors for the development of ONM-M. Thirty-eight rats (20 months old) were divided into four experimental groups: SAL, ZOL, SAL/aPDT and ZOL/aPDT. The plan of treatment lasted for 8 weeks. The rats received intraperitoneal injections of 0.45 ml of sodium chloride 0.9% (SAL and SAL/aPDT) or 0.45 ml of the same solution plus 100 ug/kg of zoledronate (ZOL and ZOL/aPDT) with an interval of 2 days between applications. The drug treatment plan lasted for 8 weeks. The rats received intraperitoneal injections of 0.45 ml of sodium chloride 0.9% (SAL and SAL/aPDT) or 0.45 ml of the same solution plus 100 ug/kg of zoledronate (ZOL and ZOL/aPDT) with an interval of 2 days between applications. After three weeks of treatment all rats were subjected to dental extraction of the first lower left molar. In groups SAL/aPDT and ZOL/aPDT the rats were subjected to aPDT sessions at 0, 2 and 4 days post extraction. After 28 postoperative days, all rats were euthanized. Histological processing was executed the hemi mandibles and histological sections were stained with hematoxylin and eosin for histological analysis and histometric...