The post-transcriptional gene silencing pathway in Eucalyptus
Autor(a) principal: | |
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Data de Publicação: | 2005 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S1415-47572005000400003 http://hdl.handle.net/11449/68571 |
Resumo: | Post-transcriptional gene silencing (PTGS) is a conserved surveillance mechanism that identifies and cleaves double-stranded RNA molecules and their cellular cognate transcripts. The RNA silencing response is actually used as a powerful technique (named RNA interference) for potent and specific inhibition of gene expression in several organisms. To identify gene products in Eucalyptus sharing similarities with enzymes involved in the PTGS pathway, we queried the expressed sequence tag database of the Brazilian Eucalyptus Genome Sequence Project Consortium (FORESTs) with the amino acid sequences of known PTGS-related proteins. Among twenty-six prospected genes, our search detected fifteen assembled sequences encoding products presenting high level of similarity (E value < 10 -40) to proteins involved in PTGS in plants and other organisms. We conclude that most of the genes known to be involved in the PTGS pathway are represented in the FORESTs database. Copyright by the Brazilian Society of Genetics. |
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The post-transcriptional gene silencing pathway in EucalyptusESTEucalyptusGene silencingPTGSRNA silencingamino acidenzymegene productamino acid sequenceBrazilcontrolled studyexpressed sequence taggenetic codegenetic databasegenetic identificationgenetic similaritymicrobial geneticsnonhumannucleotide sequenceplant geneticsposttranscriptional gene silencingprotein assemblysequence analysisPost-transcriptional gene silencing (PTGS) is a conserved surveillance mechanism that identifies and cleaves double-stranded RNA molecules and their cellular cognate transcripts. The RNA silencing response is actually used as a powerful technique (named RNA interference) for potent and specific inhibition of gene expression in several organisms. To identify gene products in Eucalyptus sharing similarities with enzymes involved in the PTGS pathway, we queried the expressed sequence tag database of the Brazilian Eucalyptus Genome Sequence Project Consortium (FORESTs) with the amino acid sequences of known PTGS-related proteins. Among twenty-six prospected genes, our search detected fifteen assembled sequences encoding products presenting high level of similarity (E value < 10 -40) to proteins involved in PTGS in plants and other organisms. We conclude that most of the genes known to be involved in the PTGS pathway are represented in the FORESTs database. Copyright by the Brazilian Society of Genetics.Universidade Estadual Paulista 'Júlio de Mesquita Filho' Instituto de Biociências Departamento de Genética, 18618-000 Botucatu, SPUniversidade Estadual de Campinas Departamento de Genética Médica, Campinas, SPUniversidade Estadual Paulista 'Júlio de Mesquita Filho' Instituto de Biociências Departamento de Genética, 18618-000 Botucatu, SPUniversidade Estadual Paulista (Unesp)Universidade Estadual de Campinas (UNICAMP)Sassaki, Flávio Tetsuo [UNESP]Campos-Pereira, Tiagode Godoy Maia, Ivan [UNESP]2014-05-27T11:21:43Z2014-05-27T11:21:43Z2005-12-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article496-500application/pdfhttp://dx.doi.org/10.1590/S1415-47572005000400003Genetics and Molecular Biology, v. 28, n. 3 SUPPL., p. 496-500, 2005.1415-47571678-4685http://hdl.handle.net/11449/6857110.1590/S1415-47572005000400003S1415-47572005000400003WOS:0002043898000032-s2.0-308444411812-s2.0-30844441181.pdf8649222099176162Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengGenetics and Molecular Biology1.4930,638info:eu-repo/semantics/openAccess2023-11-15T06:13:52Zoai:repositorio.unesp.br:11449/68571Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:44:58.543921Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
The post-transcriptional gene silencing pathway in Eucalyptus |
title |
The post-transcriptional gene silencing pathway in Eucalyptus |
spellingShingle |
The post-transcriptional gene silencing pathway in Eucalyptus Sassaki, Flávio Tetsuo [UNESP] EST Eucalyptus Gene silencing PTGS RNA silencing amino acid enzyme gene product amino acid sequence Brazil controlled study expressed sequence tag genetic code genetic database genetic identification genetic similarity microbial genetics nonhuman nucleotide sequence plant genetics posttranscriptional gene silencing protein assembly sequence analysis |
title_short |
The post-transcriptional gene silencing pathway in Eucalyptus |
title_full |
The post-transcriptional gene silencing pathway in Eucalyptus |
title_fullStr |
The post-transcriptional gene silencing pathway in Eucalyptus |
title_full_unstemmed |
The post-transcriptional gene silencing pathway in Eucalyptus |
title_sort |
The post-transcriptional gene silencing pathway in Eucalyptus |
author |
Sassaki, Flávio Tetsuo [UNESP] |
author_facet |
Sassaki, Flávio Tetsuo [UNESP] Campos-Pereira, Tiago de Godoy Maia, Ivan [UNESP] |
author_role |
author |
author2 |
Campos-Pereira, Tiago de Godoy Maia, Ivan [UNESP] |
author2_role |
author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Universidade Estadual de Campinas (UNICAMP) |
dc.contributor.author.fl_str_mv |
Sassaki, Flávio Tetsuo [UNESP] Campos-Pereira, Tiago de Godoy Maia, Ivan [UNESP] |
dc.subject.por.fl_str_mv |
EST Eucalyptus Gene silencing PTGS RNA silencing amino acid enzyme gene product amino acid sequence Brazil controlled study expressed sequence tag genetic code genetic database genetic identification genetic similarity microbial genetics nonhuman nucleotide sequence plant genetics posttranscriptional gene silencing protein assembly sequence analysis |
topic |
EST Eucalyptus Gene silencing PTGS RNA silencing amino acid enzyme gene product amino acid sequence Brazil controlled study expressed sequence tag genetic code genetic database genetic identification genetic similarity microbial genetics nonhuman nucleotide sequence plant genetics posttranscriptional gene silencing protein assembly sequence analysis |
description |
Post-transcriptional gene silencing (PTGS) is a conserved surveillance mechanism that identifies and cleaves double-stranded RNA molecules and their cellular cognate transcripts. The RNA silencing response is actually used as a powerful technique (named RNA interference) for potent and specific inhibition of gene expression in several organisms. To identify gene products in Eucalyptus sharing similarities with enzymes involved in the PTGS pathway, we queried the expressed sequence tag database of the Brazilian Eucalyptus Genome Sequence Project Consortium (FORESTs) with the amino acid sequences of known PTGS-related proteins. Among twenty-six prospected genes, our search detected fifteen assembled sequences encoding products presenting high level of similarity (E value < 10 -40) to proteins involved in PTGS in plants and other organisms. We conclude that most of the genes known to be involved in the PTGS pathway are represented in the FORESTs database. Copyright by the Brazilian Society of Genetics. |
publishDate |
2005 |
dc.date.none.fl_str_mv |
2005-12-01 2014-05-27T11:21:43Z 2014-05-27T11:21:43Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S1415-47572005000400003 Genetics and Molecular Biology, v. 28, n. 3 SUPPL., p. 496-500, 2005. 1415-4757 1678-4685 http://hdl.handle.net/11449/68571 10.1590/S1415-47572005000400003 S1415-47572005000400003 WOS:000204389800003 2-s2.0-30844441181 2-s2.0-30844441181.pdf 8649222099176162 |
url |
http://dx.doi.org/10.1590/S1415-47572005000400003 http://hdl.handle.net/11449/68571 |
identifier_str_mv |
Genetics and Molecular Biology, v. 28, n. 3 SUPPL., p. 496-500, 2005. 1415-4757 1678-4685 10.1590/S1415-47572005000400003 S1415-47572005000400003 WOS:000204389800003 2-s2.0-30844441181 2-s2.0-30844441181.pdf 8649222099176162 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Genetics and Molecular Biology 1.493 0,638 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
496-500 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128226371829760 |