Linking maximal shear rate and energy dissipation/circulation function in airlift bioreactors
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bej.2021.108308 http://hdl.handle.net/11449/223070 |
Resumo: | The interest in pneumatic bioreactors is related to their good mass transfer capacity, as well as lower operating costs, due to the simple mechanical structure. Knowledge of the transport phenomena and hydrodynamics of bioreactors is important to enable definition of the best bioreactor model and operating conditions for a specific bioprocess, with the imposed shear being one of the most difficult to quantify. For stirred tanks, the fragmentation of microorganisms has been well correlated with a hydrodynamic parameter called the “energy dissipation/circulation function” (EDCF). However, there have been no estimates of the EDCF for pneumatic bioreactors. The present work proposes a methodology to estimate the EDCF for different pneumatic bioreactors and operating conditions using two approaches: Computational Fluid Dynamics and, comparison of pellet fragmentation in conventional and pneumatic bioreactors. First, the volume of higher energy dissipation was estimated in 5 L pneumatic bioreactors, exhibiting values ranging from 57.9 to 106.5 cm³. Subsequently, EDCF values were estimated, ranging from 0.71 to 9.1 kW·m−3·s−1 for pneumatic bioreactors. Finally, these values were validated by pellet fragmentation assays in stirred tank and pneumatic bioreactors under conditions of similar fragmentation, that resulted in similar EDCF values. |
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Repositório Institucional da UNESP |
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Linking maximal shear rate and energy dissipation/circulation function in airlift bioreactorsAirlift bioreactorComputational fluid dynamicsEnergy dissipation/circulation functionPellet fragmentationShear rateThe interest in pneumatic bioreactors is related to their good mass transfer capacity, as well as lower operating costs, due to the simple mechanical structure. Knowledge of the transport phenomena and hydrodynamics of bioreactors is important to enable definition of the best bioreactor model and operating conditions for a specific bioprocess, with the imposed shear being one of the most difficult to quantify. For stirred tanks, the fragmentation of microorganisms has been well correlated with a hydrodynamic parameter called the “energy dissipation/circulation function” (EDCF). However, there have been no estimates of the EDCF for pneumatic bioreactors. The present work proposes a methodology to estimate the EDCF for different pneumatic bioreactors and operating conditions using two approaches: Computational Fluid Dynamics and, comparison of pellet fragmentation in conventional and pneumatic bioreactors. First, the volume of higher energy dissipation was estimated in 5 L pneumatic bioreactors, exhibiting values ranging from 57.9 to 106.5 cm³. Subsequently, EDCF values were estimated, ranging from 0.71 to 9.1 kW·m−3·s−1 for pneumatic bioreactors. Finally, these values were validated by pellet fragmentation assays in stirred tank and pneumatic bioreactors under conditions of similar fragmentation, that resulted in similar EDCF values.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Federal Institute of Education Science and Technology of São Paulo Campus CapivariDepartment of Chemical Engineering Federal University of São Carlos, C.P. 676Chemistry and Food School Federal University of Rio GrandeBioprocess Engineering Course Natural Resources Institute Federal University of ItajubáDepartment of Bioprocesses Engineering and Biotechnology Faculty of Pharmaceutical Sciences São Paulo State UniversityDepartment of Bioprocesses Engineering and Biotechnology Faculty of Pharmaceutical Sciences São Paulo State UniversityCAPES: 001CNPq: 131780/2018-2FAPESP: 2011/23807-1FAPESP: 2012/17756-8FAPESP: 2018/11405-5FAPESP: 2020/08699-7CNPq: 310098/2017-3CNPq: 431460/2016-7CNPq: 478472/2011-0Science and Technology of São PauloUniversidade Federal de São Carlos (UFSCar)Federal University of Rio GrandeFederal University of ItajubáUniversidade Estadual Paulista (UNESP)Esperança, Mateus N.Buffo, Mariane M.Mendes, Caroline E.Rodriguez, Guilherme Y.Béttega, RodrigoBadino, Alberto C.Cerri, Marcel O. [UNESP]2022-04-28T19:48:25Z2022-04-28T19:48:25Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1016/j.bej.2021.108308Biochemical Engineering Journal, v. 178.1873-295X1369-703Xhttp://hdl.handle.net/11449/22307010.1016/j.bej.2021.1083082-s2.0-85121317692Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengBiochemical Engineering Journalinfo:eu-repo/semantics/openAccess2022-04-28T19:48:25Zoai:repositorio.unesp.br:11449/223070Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T17:03:29.796387Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Linking maximal shear rate and energy dissipation/circulation function in airlift bioreactors |
title |
Linking maximal shear rate and energy dissipation/circulation function in airlift bioreactors |
spellingShingle |
Linking maximal shear rate and energy dissipation/circulation function in airlift bioreactors Esperança, Mateus N. Airlift bioreactor Computational fluid dynamics Energy dissipation/circulation function Pellet fragmentation Shear rate |
title_short |
Linking maximal shear rate and energy dissipation/circulation function in airlift bioreactors |
title_full |
Linking maximal shear rate and energy dissipation/circulation function in airlift bioreactors |
title_fullStr |
Linking maximal shear rate and energy dissipation/circulation function in airlift bioreactors |
title_full_unstemmed |
Linking maximal shear rate and energy dissipation/circulation function in airlift bioreactors |
title_sort |
Linking maximal shear rate and energy dissipation/circulation function in airlift bioreactors |
author |
Esperança, Mateus N. |
author_facet |
Esperança, Mateus N. Buffo, Mariane M. Mendes, Caroline E. Rodriguez, Guilherme Y. Béttega, Rodrigo Badino, Alberto C. Cerri, Marcel O. [UNESP] |
author_role |
author |
author2 |
Buffo, Mariane M. Mendes, Caroline E. Rodriguez, Guilherme Y. Béttega, Rodrigo Badino, Alberto C. Cerri, Marcel O. [UNESP] |
author2_role |
author author author author author author |
dc.contributor.none.fl_str_mv |
Science and Technology of São Paulo Universidade Federal de São Carlos (UFSCar) Federal University of Rio Grande Federal University of Itajubá Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Esperança, Mateus N. Buffo, Mariane M. Mendes, Caroline E. Rodriguez, Guilherme Y. Béttega, Rodrigo Badino, Alberto C. Cerri, Marcel O. [UNESP] |
dc.subject.por.fl_str_mv |
Airlift bioreactor Computational fluid dynamics Energy dissipation/circulation function Pellet fragmentation Shear rate |
topic |
Airlift bioreactor Computational fluid dynamics Energy dissipation/circulation function Pellet fragmentation Shear rate |
description |
The interest in pneumatic bioreactors is related to their good mass transfer capacity, as well as lower operating costs, due to the simple mechanical structure. Knowledge of the transport phenomena and hydrodynamics of bioreactors is important to enable definition of the best bioreactor model and operating conditions for a specific bioprocess, with the imposed shear being one of the most difficult to quantify. For stirred tanks, the fragmentation of microorganisms has been well correlated with a hydrodynamic parameter called the “energy dissipation/circulation function” (EDCF). However, there have been no estimates of the EDCF for pneumatic bioreactors. The present work proposes a methodology to estimate the EDCF for different pneumatic bioreactors and operating conditions using two approaches: Computational Fluid Dynamics and, comparison of pellet fragmentation in conventional and pneumatic bioreactors. First, the volume of higher energy dissipation was estimated in 5 L pneumatic bioreactors, exhibiting values ranging from 57.9 to 106.5 cm³. Subsequently, EDCF values were estimated, ranging from 0.71 to 9.1 kW·m−3·s−1 for pneumatic bioreactors. Finally, these values were validated by pellet fragmentation assays in stirred tank and pneumatic bioreactors under conditions of similar fragmentation, that resulted in similar EDCF values. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:48:25Z 2022-04-28T19:48:25Z 2022-01-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bej.2021.108308 Biochemical Engineering Journal, v. 178. 1873-295X 1369-703X http://hdl.handle.net/11449/223070 10.1016/j.bej.2021.108308 2-s2.0-85121317692 |
url |
http://dx.doi.org/10.1016/j.bej.2021.108308 http://hdl.handle.net/11449/223070 |
identifier_str_mv |
Biochemical Engineering Journal, v. 178. 1873-295X 1369-703X 10.1016/j.bej.2021.108308 2-s2.0-85121317692 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Biochemical Engineering Journal |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128224641679360 |