High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats

Detalhes bibliográficos
Autor(a) principal: Gasparini, Patrícia Vasconcelos Fontana
Data de Publicação: 2021
Outros Autores: Matias, Amanda Martins, Torezani-Sales, Suellem, Kobi, Jéssika Butcovsky Botto Sarter, Siqueira, Juliana Silva [UNESP], Corrêa, Camila Renata [UNESP], Leopoldo, Ana Paula Lima, Leopoldo, André Soares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.33594/000000441
http://hdl.handle.net/11449/222844
Resumo: Background/Aims: Oxidative stress is associated with cardiometabolic alterations, and the involvement of excess glucose and fatty acids has been demonstrated in this process. Thus, the aim of this study was to investigate the effects of different hypercaloric diets on cardiac oxidative stress. Methods: Wistar rats were randomized into four groups: control (C), high-sucrose (HS), high-fat (HF), and high-fat with sucrose (HFS). Nutritional assessment, food profiles, histological analysis, comorbidities, and cardiovascular characteristics were determined. Cardiac oxidative stress was analyzed by malondialdehyde (MDA) and carbonylated proteins, and the cardiac protein expression levels of type 1 angiotensin receptor (AT-1), nicotinamide adenine dinucleotide phosphate oxidase 2 (Nox2), superoxide dismutase (SOD 1 e 2), glutathione peroxidase (GPX), and catalase (CAT) were determined by western blot. Results: The HF group showed an increase in adiposity; however, it did not present adipocyte hypertrophy and comorbidities. Cardiac MDA and carbonylated protein levels were higher in the HF and HFS compared with the C group. The levels of oxidant and antioxidant proteins showed no difference between the groups. Conclusion: HF and HFS dietary interventions promoted cardiac oxidative stress, in the presence and absence of obesity, respectively. However, this process was neither mediated by the pro-oxidants AT1 and Nox2, nor by the quantitative reduction of antioxidant enzymes.
id UNSP_56d5da0f113f3b7620052e734632a59b
oai_identifier_str oai:repositorio.unesp.br:11449/222844
network_acronym_str UNSP
network_name_str Repositório Institucional da UNESP
repository_id_str 2946
spelling High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats1 angiotensin receptorCardiacHypercaloric dietsNox2Oxidative stressBackground/Aims: Oxidative stress is associated with cardiometabolic alterations, and the involvement of excess glucose and fatty acids has been demonstrated in this process. Thus, the aim of this study was to investigate the effects of different hypercaloric diets on cardiac oxidative stress. Methods: Wistar rats were randomized into four groups: control (C), high-sucrose (HS), high-fat (HF), and high-fat with sucrose (HFS). Nutritional assessment, food profiles, histological analysis, comorbidities, and cardiovascular characteristics were determined. Cardiac oxidative stress was analyzed by malondialdehyde (MDA) and carbonylated proteins, and the cardiac protein expression levels of type 1 angiotensin receptor (AT-1), nicotinamide adenine dinucleotide phosphate oxidase 2 (Nox2), superoxide dismutase (SOD 1 e 2), glutathione peroxidase (GPX), and catalase (CAT) were determined by western blot. Results: The HF group showed an increase in adiposity; however, it did not present adipocyte hypertrophy and comorbidities. Cardiac MDA and carbonylated protein levels were higher in the HF and HFS compared with the C group. The levels of oxidant and antioxidant proteins showed no difference between the groups. Conclusion: HF and HFS dietary interventions promoted cardiac oxidative stress, in the presence and absence of obesity, respectively. However, this process was neither mediated by the pro-oxidants AT1 and Nox2, nor by the quantitative reduction of antioxidant enzymes.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Postgraduate Program in Nutrition and Health Health Sciences Center Federal University of Espírito Santo, Espírito SantoSão Paulo State University (UNESP) Medical School, São PauloCenter for Physical Education and Sports Federal University of Espírito Santo, Espírito SantoSão Paulo State University (UNESP) Medical School, São PauloCNPq: 402090/2016-0Federal University of Espírito SantoUniversidade Estadual Paulista (UNESP)Gasparini, Patrícia Vasconcelos FontanaMatias, Amanda MartinsTorezani-Sales, SuellemKobi, Jéssika Butcovsky Botto SarterSiqueira, Juliana Silva [UNESP]Corrêa, Camila Renata [UNESP]Leopoldo, Ana Paula LimaLeopoldo, André Soares2022-04-28T19:47:07Z2022-04-28T19:47:07Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article618-634http://dx.doi.org/10.33594/000000441Cellular Physiology and Biochemistry, v. 55, n. 5, p. 618-634, 2021.1421-97781015-8987http://hdl.handle.net/11449/22284410.33594/0000004412-s2.0-85118921478Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCellular Physiology and Biochemistryinfo:eu-repo/semantics/openAccess2022-04-28T19:47:07Zoai:repositorio.unesp.br:11449/222844Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:24:55.826351Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats
title High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats
spellingShingle High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats
Gasparini, Patrícia Vasconcelos Fontana
1 angiotensin receptor
Cardiac
Hypercaloric diets
Nox2
Oxidative stress
title_short High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats
title_full High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats
title_fullStr High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats
title_full_unstemmed High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats
title_sort High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats
author Gasparini, Patrícia Vasconcelos Fontana
author_facet Gasparini, Patrícia Vasconcelos Fontana
Matias, Amanda Martins
Torezani-Sales, Suellem
Kobi, Jéssika Butcovsky Botto Sarter
Siqueira, Juliana Silva [UNESP]
Corrêa, Camila Renata [UNESP]
Leopoldo, Ana Paula Lima
Leopoldo, André Soares
author_role author
author2 Matias, Amanda Martins
Torezani-Sales, Suellem
Kobi, Jéssika Butcovsky Botto Sarter
Siqueira, Juliana Silva [UNESP]
Corrêa, Camila Renata [UNESP]
Leopoldo, Ana Paula Lima
Leopoldo, André Soares
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Federal University of Espírito Santo
Universidade Estadual Paulista (UNESP)
dc.contributor.author.fl_str_mv Gasparini, Patrícia Vasconcelos Fontana
Matias, Amanda Martins
Torezani-Sales, Suellem
Kobi, Jéssika Butcovsky Botto Sarter
Siqueira, Juliana Silva [UNESP]
Corrêa, Camila Renata [UNESP]
Leopoldo, Ana Paula Lima
Leopoldo, André Soares
dc.subject.por.fl_str_mv 1 angiotensin receptor
Cardiac
Hypercaloric diets
Nox2
Oxidative stress
topic 1 angiotensin receptor
Cardiac
Hypercaloric diets
Nox2
Oxidative stress
description Background/Aims: Oxidative stress is associated with cardiometabolic alterations, and the involvement of excess glucose and fatty acids has been demonstrated in this process. Thus, the aim of this study was to investigate the effects of different hypercaloric diets on cardiac oxidative stress. Methods: Wistar rats were randomized into four groups: control (C), high-sucrose (HS), high-fat (HF), and high-fat with sucrose (HFS). Nutritional assessment, food profiles, histological analysis, comorbidities, and cardiovascular characteristics were determined. Cardiac oxidative stress was analyzed by malondialdehyde (MDA) and carbonylated proteins, and the cardiac protein expression levels of type 1 angiotensin receptor (AT-1), nicotinamide adenine dinucleotide phosphate oxidase 2 (Nox2), superoxide dismutase (SOD 1 e 2), glutathione peroxidase (GPX), and catalase (CAT) were determined by western blot. Results: The HF group showed an increase in adiposity; however, it did not present adipocyte hypertrophy and comorbidities. Cardiac MDA and carbonylated protein levels were higher in the HF and HFS compared with the C group. The levels of oxidant and antioxidant proteins showed no difference between the groups. Conclusion: HF and HFS dietary interventions promoted cardiac oxidative stress, in the presence and absence of obesity, respectively. However, this process was neither mediated by the pro-oxidants AT1 and Nox2, nor by the quantitative reduction of antioxidant enzymes.
publishDate 2021
dc.date.none.fl_str_mv 2021-01-01
2022-04-28T19:47:07Z
2022-04-28T19:47:07Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.33594/000000441
Cellular Physiology and Biochemistry, v. 55, n. 5, p. 618-634, 2021.
1421-9778
1015-8987
http://hdl.handle.net/11449/222844
10.33594/000000441
2-s2.0-85118921478
url http://dx.doi.org/10.33594/000000441
http://hdl.handle.net/11449/222844
identifier_str_mv Cellular Physiology and Biochemistry, v. 55, n. 5, p. 618-634, 2021.
1421-9778
1015-8987
10.33594/000000441
2-s2.0-85118921478
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cellular Physiology and Biochemistry
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 618-634
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
_version_ 1808128646323372032