High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.33594/000000441 http://hdl.handle.net/11449/222844 |
Resumo: | Background/Aims: Oxidative stress is associated with cardiometabolic alterations, and the involvement of excess glucose and fatty acids has been demonstrated in this process. Thus, the aim of this study was to investigate the effects of different hypercaloric diets on cardiac oxidative stress. Methods: Wistar rats were randomized into four groups: control (C), high-sucrose (HS), high-fat (HF), and high-fat with sucrose (HFS). Nutritional assessment, food profiles, histological analysis, comorbidities, and cardiovascular characteristics were determined. Cardiac oxidative stress was analyzed by malondialdehyde (MDA) and carbonylated proteins, and the cardiac protein expression levels of type 1 angiotensin receptor (AT-1), nicotinamide adenine dinucleotide phosphate oxidase 2 (Nox2), superoxide dismutase (SOD 1 e 2), glutathione peroxidase (GPX), and catalase (CAT) were determined by western blot. Results: The HF group showed an increase in adiposity; however, it did not present adipocyte hypertrophy and comorbidities. Cardiac MDA and carbonylated protein levels were higher in the HF and HFS compared with the C group. The levels of oxidant and antioxidant proteins showed no difference between the groups. Conclusion: HF and HFS dietary interventions promoted cardiac oxidative stress, in the presence and absence of obesity, respectively. However, this process was neither mediated by the pro-oxidants AT1 and Nox2, nor by the quantitative reduction of antioxidant enzymes. |
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High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats1 angiotensin receptorCardiacHypercaloric dietsNox2Oxidative stressBackground/Aims: Oxidative stress is associated with cardiometabolic alterations, and the involvement of excess glucose and fatty acids has been demonstrated in this process. Thus, the aim of this study was to investigate the effects of different hypercaloric diets on cardiac oxidative stress. Methods: Wistar rats were randomized into four groups: control (C), high-sucrose (HS), high-fat (HF), and high-fat with sucrose (HFS). Nutritional assessment, food profiles, histological analysis, comorbidities, and cardiovascular characteristics were determined. Cardiac oxidative stress was analyzed by malondialdehyde (MDA) and carbonylated proteins, and the cardiac protein expression levels of type 1 angiotensin receptor (AT-1), nicotinamide adenine dinucleotide phosphate oxidase 2 (Nox2), superoxide dismutase (SOD 1 e 2), glutathione peroxidase (GPX), and catalase (CAT) were determined by western blot. Results: The HF group showed an increase in adiposity; however, it did not present adipocyte hypertrophy and comorbidities. Cardiac MDA and carbonylated protein levels were higher in the HF and HFS compared with the C group. The levels of oxidant and antioxidant proteins showed no difference between the groups. Conclusion: HF and HFS dietary interventions promoted cardiac oxidative stress, in the presence and absence of obesity, respectively. However, this process was neither mediated by the pro-oxidants AT1 and Nox2, nor by the quantitative reduction of antioxidant enzymes.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Postgraduate Program in Nutrition and Health Health Sciences Center Federal University of Espírito Santo, Espírito SantoSão Paulo State University (UNESP) Medical School, São PauloCenter for Physical Education and Sports Federal University of Espírito Santo, Espírito SantoSão Paulo State University (UNESP) Medical School, São PauloCNPq: 402090/2016-0Federal University of Espírito SantoUniversidade Estadual Paulista (UNESP)Gasparini, Patrícia Vasconcelos FontanaMatias, Amanda MartinsTorezani-Sales, SuellemKobi, Jéssika Butcovsky Botto SarterSiqueira, Juliana Silva [UNESP]Corrêa, Camila Renata [UNESP]Leopoldo, Ana Paula LimaLeopoldo, André Soares2022-04-28T19:47:07Z2022-04-28T19:47:07Z2021-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article618-634http://dx.doi.org/10.33594/000000441Cellular Physiology and Biochemistry, v. 55, n. 5, p. 618-634, 2021.1421-97781015-8987http://hdl.handle.net/11449/22284410.33594/0000004412-s2.0-85118921478Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCellular Physiology and Biochemistryinfo:eu-repo/semantics/openAccess2022-04-28T19:47:07Zoai:repositorio.unesp.br:11449/222844Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:24:55.826351Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats |
title |
High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats |
spellingShingle |
High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats Gasparini, Patrícia Vasconcelos Fontana 1 angiotensin receptor Cardiac Hypercaloric diets Nox2 Oxidative stress |
title_short |
High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats |
title_full |
High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats |
title_fullStr |
High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats |
title_full_unstemmed |
High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats |
title_sort |
High-fat and combined high-fat and sucrose diets promote cardiac oxidative stress independent of Nox2 redox regulation and obesity in rats |
author |
Gasparini, Patrícia Vasconcelos Fontana |
author_facet |
Gasparini, Patrícia Vasconcelos Fontana Matias, Amanda Martins Torezani-Sales, Suellem Kobi, Jéssika Butcovsky Botto Sarter Siqueira, Juliana Silva [UNESP] Corrêa, Camila Renata [UNESP] Leopoldo, Ana Paula Lima Leopoldo, André Soares |
author_role |
author |
author2 |
Matias, Amanda Martins Torezani-Sales, Suellem Kobi, Jéssika Butcovsky Botto Sarter Siqueira, Juliana Silva [UNESP] Corrêa, Camila Renata [UNESP] Leopoldo, Ana Paula Lima Leopoldo, André Soares |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Federal University of Espírito Santo Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Gasparini, Patrícia Vasconcelos Fontana Matias, Amanda Martins Torezani-Sales, Suellem Kobi, Jéssika Butcovsky Botto Sarter Siqueira, Juliana Silva [UNESP] Corrêa, Camila Renata [UNESP] Leopoldo, Ana Paula Lima Leopoldo, André Soares |
dc.subject.por.fl_str_mv |
1 angiotensin receptor Cardiac Hypercaloric diets Nox2 Oxidative stress |
topic |
1 angiotensin receptor Cardiac Hypercaloric diets Nox2 Oxidative stress |
description |
Background/Aims: Oxidative stress is associated with cardiometabolic alterations, and the involvement of excess glucose and fatty acids has been demonstrated in this process. Thus, the aim of this study was to investigate the effects of different hypercaloric diets on cardiac oxidative stress. Methods: Wistar rats were randomized into four groups: control (C), high-sucrose (HS), high-fat (HF), and high-fat with sucrose (HFS). Nutritional assessment, food profiles, histological analysis, comorbidities, and cardiovascular characteristics were determined. Cardiac oxidative stress was analyzed by malondialdehyde (MDA) and carbonylated proteins, and the cardiac protein expression levels of type 1 angiotensin receptor (AT-1), nicotinamide adenine dinucleotide phosphate oxidase 2 (Nox2), superoxide dismutase (SOD 1 e 2), glutathione peroxidase (GPX), and catalase (CAT) were determined by western blot. Results: The HF group showed an increase in adiposity; however, it did not present adipocyte hypertrophy and comorbidities. Cardiac MDA and carbonylated protein levels were higher in the HF and HFS compared with the C group. The levels of oxidant and antioxidant proteins showed no difference between the groups. Conclusion: HF and HFS dietary interventions promoted cardiac oxidative stress, in the presence and absence of obesity, respectively. However, this process was neither mediated by the pro-oxidants AT1 and Nox2, nor by the quantitative reduction of antioxidant enzymes. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-01-01 2022-04-28T19:47:07Z 2022-04-28T19:47:07Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.33594/000000441 Cellular Physiology and Biochemistry, v. 55, n. 5, p. 618-634, 2021. 1421-9778 1015-8987 http://hdl.handle.net/11449/222844 10.33594/000000441 2-s2.0-85118921478 |
url |
http://dx.doi.org/10.33594/000000441 http://hdl.handle.net/11449/222844 |
identifier_str_mv |
Cellular Physiology and Biochemistry, v. 55, n. 5, p. 618-634, 2021. 1421-9778 1015-8987 10.33594/000000441 2-s2.0-85118921478 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cellular Physiology and Biochemistry |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
618-634 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128646323372032 |