Influence of culture conditions on the secretome of mesenchymal stem cells derived from feline adipose tissue: Proteomics approach

• Autor(a) principal: Lara, Maria Laura [UNESP]
• Data de Publicação: 2023
• Outros Autores: Carvalho, Marcos Gomides [UNESP], de Souza, Fabiana Ferreira [UNESP], Schmith, Rubia Alves [UNESP], Codognoto, Viviane Maria [UNESP], De Vita, Bruna [UNESP], Freitas Dell'Aqua, Camila de Paula [UNESP], Landim, Fernada da Cruz [UNESP], Alvarenga, Marina Landim e [UNESP]
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Repositório Institucional da UNESP
• Texto Completo: http://dx.doi.org/10.1016/j.biochi.2023.03.004; http://hdl.handle.net/11449/248527
• topic: Conditioned medium; Felis catus; Hypoxia-culture; Proteomics; Serum-free-culture; Stem cell

This study aimed to describe the secretome of mesenchymal stem cells derived from feline adipose tissue (AD-MSCs) and compare the effects of different culture conditions on AD-MSC proteomics using a shotgun approach. Adipose tissue was collected from 5 female cats and prepared to culture. Conditioned media was collected at third passage, in which the cells were cultured under 4 conditions, normoxia with fetal bovine serum (N + FBS), hypoxia with FBS (H + FBS), normoxia without FBS (N – FBS), and hypoxia without FBS (H – FBS). Then, the secretome was concentrated and prepared for proteomic approaches. Secretomes cultured with FBS-free medium had more than twice identified proteins in comparison with the secretomes cultured with FBS. In contrast, hypoxic conditions did not increase protein amount and affected only a small proteome fraction. Relevant proteins were related to the extracellular matrix promoting environmental modulation, influencing cell signaling pathways, and providing a suitable environment for cell proliferation and maintenance. Moreover, other proteins were also related to cell adhesion, migration and morphogenesis. Culture conditions can influence protein abundance in AD-MSC secretome, and can give also more specificity to cell and cell-free treatments for different diseases.