Mitomycin C toxicity in rabbit corneal endothelium
Autor(a) principal: | |
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Data de Publicação: | 2009 |
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Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/s0004-27492009000200004 http://hdl.handle.net/11449/225510 |
Resumo: | Purpose: To evaluate corneal endothelium alterations after applying mitomycin C to the sclera using transmission and scanning electron microscopy, correlating alterations with time, concentration, and evaluation methods. Methods: The corneal endothelium of both eyes of 32 albino rabbits was evaluated and distributed into four groups of 8. Mitomycin C was applied under a scleral flap in the right eye for 5 minutes. Mitomycin C concentrations were 0.5 mg/ml for G1 and G2 and 0.2 mg/ml for G3 and G4. Examinations were performed 15 days after application to G1 and G3, and 30 days after application to G2 and G4. Four cornea in each group were prepared for transmission electron microscopy and four for scanning electron microscopy. Left eyes of all animals were used as controls. Results: Transmission electron microscopy showed corneal endothelium alterations in all groups: rarefied cytoplasm, dilation and fragmentation of rough endoplasmic reticulum cisternae, Golgi apparatus with cisternal dilation, reduced vacuoles, and irregularities of internal membrane more noticeable in G1 and G2. Scanning electron microscopy revealed alterations in all groups except G1: changes in the shape and size of cells and longer filopodial projections. Conclusions: 1 - Corneal endothelium alterations were seen at both 0.5 and 0.2 mg/ml concentrations and at 15 and 30 days after mytomicin C application; 2 - Alterations were more intense with higher mytomicin C concentration by transmission electron but not by scanning electron microscopy; 3 - The alterations correlated with time by scanning electron microscopy but not by transmission electron microscopy. |
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Mitomycin C toxicity in rabbit corneal endotheliumToxicidade da mitomicina C ao endotélio da córnea de coelhosAqueous humor/metabolismEndothelium, cornealMicroscopy, electron, scanningMicroscopy, electron, transmissionMitomycin/toxicityRabbitsVitreous bodyPurpose: To evaluate corneal endothelium alterations after applying mitomycin C to the sclera using transmission and scanning electron microscopy, correlating alterations with time, concentration, and evaluation methods. Methods: The corneal endothelium of both eyes of 32 albino rabbits was evaluated and distributed into four groups of 8. Mitomycin C was applied under a scleral flap in the right eye for 5 minutes. Mitomycin C concentrations were 0.5 mg/ml for G1 and G2 and 0.2 mg/ml for G3 and G4. Examinations were performed 15 days after application to G1 and G3, and 30 days after application to G2 and G4. Four cornea in each group were prepared for transmission electron microscopy and four for scanning electron microscopy. Left eyes of all animals were used as controls. Results: Transmission electron microscopy showed corneal endothelium alterations in all groups: rarefied cytoplasm, dilation and fragmentation of rough endoplasmic reticulum cisternae, Golgi apparatus with cisternal dilation, reduced vacuoles, and irregularities of internal membrane more noticeable in G1 and G2. Scanning electron microscopy revealed alterations in all groups except G1: changes in the shape and size of cells and longer filopodial projections. Conclusions: 1 - Corneal endothelium alterations were seen at both 0.5 and 0.2 mg/ml concentrations and at 15 and 30 days after mytomicin C application; 2 - Alterations were more intense with higher mytomicin C concentration by transmission electron but not by scanning electron microscopy; 3 - The alterations correlated with time by scanning electron microscopy but not by transmission electron microscopy.Adjunta do Departamento de Oftalmologia Otorrinolaringologia e Cabeça e Pescoço da Faculdade de Medicina de Botucatu Universidade Estadual Paulista - UNESP, Botucatu (SP)Departamento de Morfologia do Instituto de Biociências da UNESP, Botucatu (SP)Adjunta do Departamento de Oftalmologia Otorrinolaringologia e Cabeça e Pescoço da Faculdade de Medicina de Botucatu Universidade Estadual Paulista - UNESP, Botucatu (SP)Departamento de Morfologia do Instituto de Biociências da UNESP, Botucatu (SP)Universidade Estadual Paulista (UNESP)de Moraes Silva, Maria Rosa Bet [UNESP]Gregório, Elisa Aparecida [UNESP]2022-04-28T20:52:54Z2022-04-28T20:52:54Z2009-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article152-158http://dx.doi.org/10.1590/s0004-27492009000200004Arquivos Brasileiros de Oftalmologia, v. 72, n. 2, p. 152-158, 2009.1678-29250004-2749http://hdl.handle.net/11449/22551010.1590/s0004-274920090002000042-s2.0-67649204992Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporArquivos Brasileiros de Oftalmologiainfo:eu-repo/semantics/openAccess2024-08-16T18:43:51Zoai:repositorio.unesp.br:11449/225510Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T18:43:51Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Mitomycin C toxicity in rabbit corneal endothelium Toxicidade da mitomicina C ao endotélio da córnea de coelhos |
title |
Mitomycin C toxicity in rabbit corneal endothelium |
spellingShingle |
Mitomycin C toxicity in rabbit corneal endothelium de Moraes Silva, Maria Rosa Bet [UNESP] Aqueous humor/metabolism Endothelium, corneal Microscopy, electron, scanning Microscopy, electron, transmission Mitomycin/toxicity Rabbits Vitreous body |
title_short |
Mitomycin C toxicity in rabbit corneal endothelium |
title_full |
Mitomycin C toxicity in rabbit corneal endothelium |
title_fullStr |
Mitomycin C toxicity in rabbit corneal endothelium |
title_full_unstemmed |
Mitomycin C toxicity in rabbit corneal endothelium |
title_sort |
Mitomycin C toxicity in rabbit corneal endothelium |
author |
de Moraes Silva, Maria Rosa Bet [UNESP] |
author_facet |
de Moraes Silva, Maria Rosa Bet [UNESP] Gregório, Elisa Aparecida [UNESP] |
author_role |
author |
author2 |
Gregório, Elisa Aparecida [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
de Moraes Silva, Maria Rosa Bet [UNESP] Gregório, Elisa Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Aqueous humor/metabolism Endothelium, corneal Microscopy, electron, scanning Microscopy, electron, transmission Mitomycin/toxicity Rabbits Vitreous body |
topic |
Aqueous humor/metabolism Endothelium, corneal Microscopy, electron, scanning Microscopy, electron, transmission Mitomycin/toxicity Rabbits Vitreous body |
description |
Purpose: To evaluate corneal endothelium alterations after applying mitomycin C to the sclera using transmission and scanning electron microscopy, correlating alterations with time, concentration, and evaluation methods. Methods: The corneal endothelium of both eyes of 32 albino rabbits was evaluated and distributed into four groups of 8. Mitomycin C was applied under a scleral flap in the right eye for 5 minutes. Mitomycin C concentrations were 0.5 mg/ml for G1 and G2 and 0.2 mg/ml for G3 and G4. Examinations were performed 15 days after application to G1 and G3, and 30 days after application to G2 and G4. Four cornea in each group were prepared for transmission electron microscopy and four for scanning electron microscopy. Left eyes of all animals were used as controls. Results: Transmission electron microscopy showed corneal endothelium alterations in all groups: rarefied cytoplasm, dilation and fragmentation of rough endoplasmic reticulum cisternae, Golgi apparatus with cisternal dilation, reduced vacuoles, and irregularities of internal membrane more noticeable in G1 and G2. Scanning electron microscopy revealed alterations in all groups except G1: changes in the shape and size of cells and longer filopodial projections. Conclusions: 1 - Corneal endothelium alterations were seen at both 0.5 and 0.2 mg/ml concentrations and at 15 and 30 days after mytomicin C application; 2 - Alterations were more intense with higher mytomicin C concentration by transmission electron but not by scanning electron microscopy; 3 - The alterations correlated with time by scanning electron microscopy but not by transmission electron microscopy. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-01-01 2022-04-28T20:52:54Z 2022-04-28T20:52:54Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/s0004-27492009000200004 Arquivos Brasileiros de Oftalmologia, v. 72, n. 2, p. 152-158, 2009. 1678-2925 0004-2749 http://hdl.handle.net/11449/225510 10.1590/s0004-27492009000200004 2-s2.0-67649204992 |
url |
http://dx.doi.org/10.1590/s0004-27492009000200004 http://hdl.handle.net/11449/225510 |
identifier_str_mv |
Arquivos Brasileiros de Oftalmologia, v. 72, n. 2, p. 152-158, 2009. 1678-2925 0004-2749 10.1590/s0004-27492009000200004 2-s2.0-67649204992 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Arquivos Brasileiros de Oftalmologia |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
152-158 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128127948292096 |