Toxicidade da mitomicina C ao endotélio da córnea de coelhos
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/S0004-27492009000200004 http://hdl.handle.net/11449/12614 |
Resumo: | Purpose: To evaluate corneal endothelium alterations after applying mitomycin C to the sclera using transmission and scanning electron microscopy, correlating alterations with time, concentration, and evaluation methods. Methods: The corneal endothelium of both eyes of 32 albino rabbits was evaluated and distributed into four groups of 8. Mitomycin C was applied under a scleral flap in the right eye for 5 minutes. Mitomycin C concentrations were 0.5 mg/ml for G1 and G2 and 0.2 mg/ml for G3 and G4. Examinations were performed 15 days after application to G1 and G3, and 30 days after application to G2 and G4. Four cornea in each group were prepared for transmission electron microscopy and four for scanning electron microscopy. Left eyes of all animals were used as controls. Results: Transmission electron microscopy showed corneal endothelium alterations in all groups: rarefied cytoplasm, dilation and fragmentation of rough endoplasmic reticulum cisternae, Golgi apparatus with cisternal dilation, reduced vacuoles, and irregularities of internal membrane more noticeable in G1 and G2. Scanning electron microscopy revealed alterations in all groups except G1: changes in the shape and size of cells and longer filopodial projections. Conclusions: 1-Corneal endothelium alterations were seen at both 0.5 and 0.2 mg/ml concentrations and at 15 and 30 days after mytomicin C application; 2 - Alterations were more intense with higher mytomicin C concentration by transmission electron but not by scanning electron microscopy; 3 - The alterations correlated with time by scanning electron microscopy but not by transmission electron microscopy. |
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Toxicidade da mitomicina C ao endotélio da córnea de coelhosMitomycin C toxicity in rabbit corneal endotheliumEndothelium, cornealMitomycin/toxicityMicroscopy, electron, transmissionMicroscopy, electron, scanningAqueous humor/metabolismVitreous bodyRabbitsPurpose: To evaluate corneal endothelium alterations after applying mitomycin C to the sclera using transmission and scanning electron microscopy, correlating alterations with time, concentration, and evaluation methods. Methods: The corneal endothelium of both eyes of 32 albino rabbits was evaluated and distributed into four groups of 8. Mitomycin C was applied under a scleral flap in the right eye for 5 minutes. Mitomycin C concentrations were 0.5 mg/ml for G1 and G2 and 0.2 mg/ml for G3 and G4. Examinations were performed 15 days after application to G1 and G3, and 30 days after application to G2 and G4. Four cornea in each group were prepared for transmission electron microscopy and four for scanning electron microscopy. Left eyes of all animals were used as controls. Results: Transmission electron microscopy showed corneal endothelium alterations in all groups: rarefied cytoplasm, dilation and fragmentation of rough endoplasmic reticulum cisternae, Golgi apparatus with cisternal dilation, reduced vacuoles, and irregularities of internal membrane more noticeable in G1 and G2. Scanning electron microscopy revealed alterations in all groups except G1: changes in the shape and size of cells and longer filopodial projections. Conclusions: 1-Corneal endothelium alterations were seen at both 0.5 and 0.2 mg/ml concentrations and at 15 and 30 days after mytomicin C application; 2 - Alterations were more intense with higher mytomicin C concentration by transmission electron but not by scanning electron microscopy; 3 - The alterations correlated with time by scanning electron microscopy but not by transmission electron microscopy.UNESP, Fac Med Botucatu, Dept Oftalmol Otorrinolaringol & Cabeca & Pescoco, BR-18618970 Botucatu, SP, BrazilUNESP, Fac Med Botucatu, Dept Oftalmol Otorrinolaringol & Cabeca & Pescoco, BR-18618970 Botucatu, SP, BrazilConselho Brasileiro de OftalmologiaUniversidade Estadual Paulista (Unesp)Bet de Moraes Silva, Maria Rosa [UNESP]Gregorio, Elisa Aparecida [UNESP]2014-05-20T13:36:40Z2014-05-20T13:36:40Z2009-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article152-158application/pdfhttp://dx.doi.org/10.1590/S0004-27492009000200004Arquivos Brasileiros de Oftalmologia. São Paulo: Consel Brasil Oftalmologia, v. 72, n. 2, p. 152-158, 2009.0004-2749http://hdl.handle.net/11449/12614S0004-27492009000200004WOS:000274753900004S0004-27492009000200004.pdfWeb of Sciencereponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporArquivos Brasileiros de Oftalmologia1.0260,518info:eu-repo/semantics/openAccess2024-08-16T18:44:19Zoai:repositorio.unesp.br:11449/12614Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-16T18:44:19Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Toxicidade da mitomicina C ao endotélio da córnea de coelhos Mitomycin C toxicity in rabbit corneal endothelium |
title |
Toxicidade da mitomicina C ao endotélio da córnea de coelhos |
spellingShingle |
Toxicidade da mitomicina C ao endotélio da córnea de coelhos Bet de Moraes Silva, Maria Rosa [UNESP] Endothelium, corneal Mitomycin/toxicity Microscopy, electron, transmission Microscopy, electron, scanning Aqueous humor/metabolism Vitreous body Rabbits |
title_short |
Toxicidade da mitomicina C ao endotélio da córnea de coelhos |
title_full |
Toxicidade da mitomicina C ao endotélio da córnea de coelhos |
title_fullStr |
Toxicidade da mitomicina C ao endotélio da córnea de coelhos |
title_full_unstemmed |
Toxicidade da mitomicina C ao endotélio da córnea de coelhos |
title_sort |
Toxicidade da mitomicina C ao endotélio da córnea de coelhos |
author |
Bet de Moraes Silva, Maria Rosa [UNESP] |
author_facet |
Bet de Moraes Silva, Maria Rosa [UNESP] Gregorio, Elisa Aparecida [UNESP] |
author_role |
author |
author2 |
Gregorio, Elisa Aparecida [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Bet de Moraes Silva, Maria Rosa [UNESP] Gregorio, Elisa Aparecida [UNESP] |
dc.subject.por.fl_str_mv |
Endothelium, corneal Mitomycin/toxicity Microscopy, electron, transmission Microscopy, electron, scanning Aqueous humor/metabolism Vitreous body Rabbits |
topic |
Endothelium, corneal Mitomycin/toxicity Microscopy, electron, transmission Microscopy, electron, scanning Aqueous humor/metabolism Vitreous body Rabbits |
description |
Purpose: To evaluate corneal endothelium alterations after applying mitomycin C to the sclera using transmission and scanning electron microscopy, correlating alterations with time, concentration, and evaluation methods. Methods: The corneal endothelium of both eyes of 32 albino rabbits was evaluated and distributed into four groups of 8. Mitomycin C was applied under a scleral flap in the right eye for 5 minutes. Mitomycin C concentrations were 0.5 mg/ml for G1 and G2 and 0.2 mg/ml for G3 and G4. Examinations were performed 15 days after application to G1 and G3, and 30 days after application to G2 and G4. Four cornea in each group were prepared for transmission electron microscopy and four for scanning electron microscopy. Left eyes of all animals were used as controls. Results: Transmission electron microscopy showed corneal endothelium alterations in all groups: rarefied cytoplasm, dilation and fragmentation of rough endoplasmic reticulum cisternae, Golgi apparatus with cisternal dilation, reduced vacuoles, and irregularities of internal membrane more noticeable in G1 and G2. Scanning electron microscopy revealed alterations in all groups except G1: changes in the shape and size of cells and longer filopodial projections. Conclusions: 1-Corneal endothelium alterations were seen at both 0.5 and 0.2 mg/ml concentrations and at 15 and 30 days after mytomicin C application; 2 - Alterations were more intense with higher mytomicin C concentration by transmission electron but not by scanning electron microscopy; 3 - The alterations correlated with time by scanning electron microscopy but not by transmission electron microscopy. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-03-01 2014-05-20T13:36:40Z 2014-05-20T13:36:40Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/S0004-27492009000200004 Arquivos Brasileiros de Oftalmologia. São Paulo: Consel Brasil Oftalmologia, v. 72, n. 2, p. 152-158, 2009. 0004-2749 http://hdl.handle.net/11449/12614 S0004-27492009000200004 WOS:000274753900004 S0004-27492009000200004.pdf |
url |
http://dx.doi.org/10.1590/S0004-27492009000200004 http://hdl.handle.net/11449/12614 |
identifier_str_mv |
Arquivos Brasileiros de Oftalmologia. São Paulo: Consel Brasil Oftalmologia, v. 72, n. 2, p. 152-158, 2009. 0004-2749 S0004-27492009000200004 WOS:000274753900004 S0004-27492009000200004.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Arquivos Brasileiros de Oftalmologia 1.026 0,518 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
152-158 application/pdf |
dc.publisher.none.fl_str_mv |
Conselho Brasileiro de Oftalmologia |
publisher.none.fl_str_mv |
Conselho Brasileiro de Oftalmologia |
dc.source.none.fl_str_mv |
Web of Science reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128184664719360 |