How Does Brazilian Green Propolis Act in Normal and Tumor Cells? Identification of Compounds in or out Monocytes or HEp-2 Cells

Detalhes bibliográficos
Autor(a) principal: Farlow, Anthony [UNESP]
Data de Publicação: 2023
Outros Autores: José Crevelin, Eduardo, Basso Santiago, Karina [UNESP], de Oliveira Cardoso, Eliza [UNESP], Matsumoto da Silva, Livia [UNESP], Kenupp Bastos, Jairo, Maurício Sforcin, José [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1002/cbdv.202300348
http://hdl.handle.net/11449/247116
Resumo: The aim of this study was to identify propolis compounds after incubation of normal and tumor cells (monocytes and HEp-2 cells, respectively) with Brazilian green propolis, in the lysate and supernatant of cell cultures and within these cells by gas chromatography-mass spectrometry (GC/MS). Cinnamic acid derivatives were generally localized in the lysate of both cell lines after incubation, suggesting these compounds are actively transported across the membrane into the cytoplasm. Terpenes were also found in the lysate. Artepillin C, in contrast, was localised only in the supernatant. Some constituents were unobservable after incubation, especially in monocytes, suggesting the compounds had been degraded. Our findings shed light on the possible sites of action (intracellular or via a cell membrane protein) and the bioavailability of various constituents of propolis, as well as possible modes of delivery of bioactive constituents.
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spelling How Does Brazilian Green Propolis Act in Normal and Tumor Cells? Identification of Compounds in or out Monocytes or HEp-2 Cellschemical constituentsGC/MSHEp-2 cellsmonocytespropolisThe aim of this study was to identify propolis compounds after incubation of normal and tumor cells (monocytes and HEp-2 cells, respectively) with Brazilian green propolis, in the lysate and supernatant of cell cultures and within these cells by gas chromatography-mass spectrometry (GC/MS). Cinnamic acid derivatives were generally localized in the lysate of both cell lines after incubation, suggesting these compounds are actively transported across the membrane into the cytoplasm. Terpenes were also found in the lysate. Artepillin C, in contrast, was localised only in the supernatant. Some constituents were unobservable after incubation, especially in monocytes, suggesting the compounds had been degraded. Our findings shed light on the possible sites of action (intracellular or via a cell membrane protein) and the bioavailability of various constituents of propolis, as well as possible modes of delivery of bioactive constituents.Department of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP), Campus Botucatu, SPDepartment of Chemistry School of Philosophy Sciences and Languages University of São Paulo (USP), SPDepartment of Pharmaceutical Sciences School of Pharmaceutical Sciences University of São Paulo (USP), SPDepartment of Chemical and Biological Sciences Institute of Biosciences São Paulo State University (UNESP), Campus Botucatu, SPUniversidade Estadual Paulista (UNESP)Universidade de São Paulo (USP)Farlow, Anthony [UNESP]José Crevelin, EduardoBasso Santiago, Karina [UNESP]de Oliveira Cardoso, Eliza [UNESP]Matsumoto da Silva, Livia [UNESP]Kenupp Bastos, JairoMaurício Sforcin, José [UNESP]2023-07-29T13:06:43Z2023-07-29T13:06:43Z2023-05-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.1002/cbdv.202300348Chemistry and Biodiversity, v. 20, n. 5, 2023.1612-18801612-1872http://hdl.handle.net/11449/24711610.1002/cbdv.2023003482-s2.0-85151694560Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengChemistry and Biodiversityinfo:eu-repo/semantics/openAccess2023-07-29T13:06:43Zoai:repositorio.unesp.br:11449/247116Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T23:46:21.588143Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv How Does Brazilian Green Propolis Act in Normal and Tumor Cells? Identification of Compounds in or out Monocytes or HEp-2 Cells
title How Does Brazilian Green Propolis Act in Normal and Tumor Cells? Identification of Compounds in or out Monocytes or HEp-2 Cells
spellingShingle How Does Brazilian Green Propolis Act in Normal and Tumor Cells? Identification of Compounds in or out Monocytes or HEp-2 Cells
Farlow, Anthony [UNESP]
chemical constituents
GC/MS
HEp-2 cells
monocytes
propolis
title_short How Does Brazilian Green Propolis Act in Normal and Tumor Cells? Identification of Compounds in or out Monocytes or HEp-2 Cells
title_full How Does Brazilian Green Propolis Act in Normal and Tumor Cells? Identification of Compounds in or out Monocytes or HEp-2 Cells
title_fullStr How Does Brazilian Green Propolis Act in Normal and Tumor Cells? Identification of Compounds in or out Monocytes or HEp-2 Cells
title_full_unstemmed How Does Brazilian Green Propolis Act in Normal and Tumor Cells? Identification of Compounds in or out Monocytes or HEp-2 Cells
title_sort How Does Brazilian Green Propolis Act in Normal and Tumor Cells? Identification of Compounds in or out Monocytes or HEp-2 Cells
author Farlow, Anthony [UNESP]
author_facet Farlow, Anthony [UNESP]
José Crevelin, Eduardo
Basso Santiago, Karina [UNESP]
de Oliveira Cardoso, Eliza [UNESP]
Matsumoto da Silva, Livia [UNESP]
Kenupp Bastos, Jairo
Maurício Sforcin, José [UNESP]
author_role author
author2 José Crevelin, Eduardo
Basso Santiago, Karina [UNESP]
de Oliveira Cardoso, Eliza [UNESP]
Matsumoto da Silva, Livia [UNESP]
Kenupp Bastos, Jairo
Maurício Sforcin, José [UNESP]
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Farlow, Anthony [UNESP]
José Crevelin, Eduardo
Basso Santiago, Karina [UNESP]
de Oliveira Cardoso, Eliza [UNESP]
Matsumoto da Silva, Livia [UNESP]
Kenupp Bastos, Jairo
Maurício Sforcin, José [UNESP]
dc.subject.por.fl_str_mv chemical constituents
GC/MS
HEp-2 cells
monocytes
propolis
topic chemical constituents
GC/MS
HEp-2 cells
monocytes
propolis
description The aim of this study was to identify propolis compounds after incubation of normal and tumor cells (monocytes and HEp-2 cells, respectively) with Brazilian green propolis, in the lysate and supernatant of cell cultures and within these cells by gas chromatography-mass spectrometry (GC/MS). Cinnamic acid derivatives were generally localized in the lysate of both cell lines after incubation, suggesting these compounds are actively transported across the membrane into the cytoplasm. Terpenes were also found in the lysate. Artepillin C, in contrast, was localised only in the supernatant. Some constituents were unobservable after incubation, especially in monocytes, suggesting the compounds had been degraded. Our findings shed light on the possible sites of action (intracellular or via a cell membrane protein) and the bioavailability of various constituents of propolis, as well as possible modes of delivery of bioactive constituents.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:06:43Z
2023-07-29T13:06:43Z
2023-05-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1002/cbdv.202300348
Chemistry and Biodiversity, v. 20, n. 5, 2023.
1612-1880
1612-1872
http://hdl.handle.net/11449/247116
10.1002/cbdv.202300348
2-s2.0-85151694560
url http://dx.doi.org/10.1002/cbdv.202300348
http://hdl.handle.net/11449/247116
identifier_str_mv Chemistry and Biodiversity, v. 20, n. 5, 2023.
1612-1880
1612-1872
10.1002/cbdv.202300348
2-s2.0-85151694560
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Chemistry and Biodiversity
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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