In vitro toxicogenomic activity of an MTA/salicylate-based endodontic sealer
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.toxrep.2022.05.004 http://hdl.handle.net/11449/241833 |
Resumo: | This study was designed to investigate whether mineral trioxide aggregate (MTA) Fillapex®, an MTA/salicylate-based endodontic sealer, exerts cytotoxic and toxicogenomic effects on human gingival fibroblasts (HGFs). HGFs were exposed in vitro to MTA Fillapex® at concentrations of 5%, 10%, 20%, and 40% for 24 h. Cytotoxicity, cell survival (5 days), cell cycle kinetics (flow cytometry), genotoxicity (comet assay), and gene (TP53, BAX, and BCL2) expression profiles were evaluated using reverse-transcriptase quantitative polymerase chain reaction. MTA Fillapex® was cytotoxic to HGFs at the two highest concentrations (20% and 40%), and cell survival decreased after 5 days treatment only with 40% concentration. After MTA Fillapex® treatment, there was an increase in the expression of apoptosis-related genes BAX, BCL2, and TP53, but no increase in DNA damage. Cement also induced changes in cell cycle kinetics, apoptosis, and necrosis rates. The data show the ability of MTA Fillapex® endodontic sealer to induce cellular and genetic alterations in HGFs. Our findings suggest that this compound should be used with caution to avoid health-related risks to the buccal tissue. |
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Repositório Institucional da UNESP |
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In vitro toxicogenomic activity of an MTA/salicylate-based endodontic sealerApoptosisCell cycle kineticsCytotoxicityGene expressionGenotoxicityMTA FillapexThis study was designed to investigate whether mineral trioxide aggregate (MTA) Fillapex®, an MTA/salicylate-based endodontic sealer, exerts cytotoxic and toxicogenomic effects on human gingival fibroblasts (HGFs). HGFs were exposed in vitro to MTA Fillapex® at concentrations of 5%, 10%, 20%, and 40% for 24 h. Cytotoxicity, cell survival (5 days), cell cycle kinetics (flow cytometry), genotoxicity (comet assay), and gene (TP53, BAX, and BCL2) expression profiles were evaluated using reverse-transcriptase quantitative polymerase chain reaction. MTA Fillapex® was cytotoxic to HGFs at the two highest concentrations (20% and 40%), and cell survival decreased after 5 days treatment only with 40% concentration. After MTA Fillapex® treatment, there was an increase in the expression of apoptosis-related genes BAX, BCL2, and TP53, but no increase in DNA damage. Cement also induced changes in cell cycle kinetics, apoptosis, and necrosis rates. The data show the ability of MTA Fillapex® endodontic sealer to induce cellular and genetic alterations in HGFs. Our findings suggest that this compound should be used with caution to avoid health-related risks to the buccal tissue.Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State University (UNESP) Medical School PA, SPSão Paulo State University (UNESP) Medical School PA, SPCNPq: CNPq 303435/2016-0Universidade Estadual Paulista (UNESP)Leme, Kamila Sauer Veiga [UNESP]Salvadori, Daisy Maria Fávero [UNESP]2023-03-02T00:29:49Z2023-03-02T00:29:49Z2022-01-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1076-1081http://dx.doi.org/10.1016/j.toxrep.2022.05.004Toxicology Reports, v. 9, p. 1076-1081.2214-7500http://hdl.handle.net/11449/24183310.1016/j.toxrep.2022.05.0042-s2.0-85129928835Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengToxicology Reportsinfo:eu-repo/semantics/openAccess2023-03-02T00:29:49Zoai:repositorio.unesp.br:11449/241833Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-03-02T00:29:49Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
In vitro toxicogenomic activity of an MTA/salicylate-based endodontic sealer |
title |
In vitro toxicogenomic activity of an MTA/salicylate-based endodontic sealer |
spellingShingle |
In vitro toxicogenomic activity of an MTA/salicylate-based endodontic sealer Leme, Kamila Sauer Veiga [UNESP] Apoptosis Cell cycle kinetics Cytotoxicity Gene expression Genotoxicity MTA Fillapex |
title_short |
In vitro toxicogenomic activity of an MTA/salicylate-based endodontic sealer |
title_full |
In vitro toxicogenomic activity of an MTA/salicylate-based endodontic sealer |
title_fullStr |
In vitro toxicogenomic activity of an MTA/salicylate-based endodontic sealer |
title_full_unstemmed |
In vitro toxicogenomic activity of an MTA/salicylate-based endodontic sealer |
title_sort |
In vitro toxicogenomic activity of an MTA/salicylate-based endodontic sealer |
author |
Leme, Kamila Sauer Veiga [UNESP] |
author_facet |
Leme, Kamila Sauer Veiga [UNESP] Salvadori, Daisy Maria Fávero [UNESP] |
author_role |
author |
author2 |
Salvadori, Daisy Maria Fávero [UNESP] |
author2_role |
author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Leme, Kamila Sauer Veiga [UNESP] Salvadori, Daisy Maria Fávero [UNESP] |
dc.subject.por.fl_str_mv |
Apoptosis Cell cycle kinetics Cytotoxicity Gene expression Genotoxicity MTA Fillapex |
topic |
Apoptosis Cell cycle kinetics Cytotoxicity Gene expression Genotoxicity MTA Fillapex |
description |
This study was designed to investigate whether mineral trioxide aggregate (MTA) Fillapex®, an MTA/salicylate-based endodontic sealer, exerts cytotoxic and toxicogenomic effects on human gingival fibroblasts (HGFs). HGFs were exposed in vitro to MTA Fillapex® at concentrations of 5%, 10%, 20%, and 40% for 24 h. Cytotoxicity, cell survival (5 days), cell cycle kinetics (flow cytometry), genotoxicity (comet assay), and gene (TP53, BAX, and BCL2) expression profiles were evaluated using reverse-transcriptase quantitative polymerase chain reaction. MTA Fillapex® was cytotoxic to HGFs at the two highest concentrations (20% and 40%), and cell survival decreased after 5 days treatment only with 40% concentration. After MTA Fillapex® treatment, there was an increase in the expression of apoptosis-related genes BAX, BCL2, and TP53, but no increase in DNA damage. Cement also induced changes in cell cycle kinetics, apoptosis, and necrosis rates. The data show the ability of MTA Fillapex® endodontic sealer to induce cellular and genetic alterations in HGFs. Our findings suggest that this compound should be used with caution to avoid health-related risks to the buccal tissue. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-01-01 2023-03-02T00:29:49Z 2023-03-02T00:29:49Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.toxrep.2022.05.004 Toxicology Reports, v. 9, p. 1076-1081. 2214-7500 http://hdl.handle.net/11449/241833 10.1016/j.toxrep.2022.05.004 2-s2.0-85129928835 |
url |
http://dx.doi.org/10.1016/j.toxrep.2022.05.004 http://hdl.handle.net/11449/241833 |
identifier_str_mv |
Toxicology Reports, v. 9, p. 1076-1081. 2214-7500 10.1016/j.toxrep.2022.05.004 2-s2.0-85129928835 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Toxicology Reports |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1076-1081 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1803649548587892736 |