Hepatic steatosis alters cellular recruitment during induced aerocystitis in Nile tilapia
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1590/1678-5150-PVB-5533 http://hdl.handle.net/11449/187035 |
Resumo: | The study evaluated the hepatotoxicity induced by CCl4 during the glucocorticoid effect of dexamethasone (DEX) on the pathophysiology of the acute inflammatory reaction in Nile tilapia, Oreochromis niloticus, correlating hepatic functionality with cellular accumulation kinetics in infectious aerocystitis. Eighty- four Nile tilapia were distributed into four treatments: control, CCl4, DEX and CCl4 + DEX. Seven tilapia were sampled per treatment in three periods: 6, 24 and 48h after induction of inflammation. CCl4 was used in a single dose of 0.5mL/kg intraperitoneally to cause hepatic disorder. Aeromonas hydrophila inoculum was used to induce aerocystitis. Dexamethasone was administered intramuscularly at the dose of 2mg/kg b. w. The results revealed a higher serum aspartate transaminase (AST) activity associated with greater somatic liver alteration, being these findings inversely proportional to the cellular accumulation in the inflammatory focus, demonstrating a lower number of inflammatory cells in the animals affected with a higher degree of hepatic disorders induced by CCl4. The histopathological study revealed transient degenerative changes in the most acute phase, as livers of tilapia showed accumulation of lipids in hepatocytes 6 hours after administration of CCl4, and this fatty degeneration was no longer observed in 24 and 48h. However, administration of CCl4 in Nile tilapia resulted in acute and transient liver degeneration, characterized by accumulation of fat in hepatocytes, increased AST in the blood and hepatomegaly. With liver dysfunction there was compromise of cellular recruitment in infectious aerocystitis, indicating that there is liver involvement in the innate immune response in tilapia. |
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Hepatic steatosis alters cellular recruitment during induced aerocystitis in Nile tilapiaEsteatose hepática altera acúmulo celular em tilápias do Nilo durante aerocistite infecciosaAcute inflammationAerocystitisCarbon tetrachlorideCichlid fishGlucocorticoidHepatic steatosisTilapiaThe study evaluated the hepatotoxicity induced by CCl4 during the glucocorticoid effect of dexamethasone (DEX) on the pathophysiology of the acute inflammatory reaction in Nile tilapia, Oreochromis niloticus, correlating hepatic functionality with cellular accumulation kinetics in infectious aerocystitis. Eighty- four Nile tilapia were distributed into four treatments: control, CCl4, DEX and CCl4 + DEX. Seven tilapia were sampled per treatment in three periods: 6, 24 and 48h after induction of inflammation. CCl4 was used in a single dose of 0.5mL/kg intraperitoneally to cause hepatic disorder. Aeromonas hydrophila inoculum was used to induce aerocystitis. Dexamethasone was administered intramuscularly at the dose of 2mg/kg b. w. The results revealed a higher serum aspartate transaminase (AST) activity associated with greater somatic liver alteration, being these findings inversely proportional to the cellular accumulation in the inflammatory focus, demonstrating a lower number of inflammatory cells in the animals affected with a higher degree of hepatic disorders induced by CCl4. The histopathological study revealed transient degenerative changes in the most acute phase, as livers of tilapia showed accumulation of lipids in hepatocytes 6 hours after administration of CCl4, and this fatty degeneration was no longer observed in 24 and 48h. However, administration of CCl4 in Nile tilapia resulted in acute and transient liver degeneration, characterized by accumulation of fat in hepatocytes, increased AST in the blood and hepatomegaly. With liver dysfunction there was compromise of cellular recruitment in infectious aerocystitis, indicating that there is liver involvement in the innate immune response in tilapia.Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), Via de acesso Prof. Paulo Donato Castellane s/nMédica Veterinária Autônoma, Rua Daniel de Oliveira Carvalho 140, Cx. Postal 525Laboratório de Patologia Clínica Max Vet Hospital Veterinário, Rua Tibúrcio de Souza 1490Faculdade de Ciências Agrárias e Veterinárias (FCAV) Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp), Via de acesso Prof. Paulo Donato Castellane s/nUniversidade Estadual Paulista (Unesp)Médica Veterinária AutônomaLaboratório de Patologia Clínica Max Vet Hospital VeterinárioMoraes, Alessandra C. [UNESP]Prado, Ed J.R. [UNESP]Foz, Elizabeth P.Barbuio, Roberto [UNESP]Faria, Vanessa P.Belo, Marco A.A. [UNESP]2019-10-06T15:23:30Z2019-10-06T15:23:30Z2018-08-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1570-1576application/pdfhttp://dx.doi.org/10.1590/1678-5150-PVB-5533Pesquisa Veterinaria Brasileira, v. 38, n. 8, p. 1570-1576, 2018.1678-51500100-736Xhttp://hdl.handle.net/11449/18703510.1590/1678-5150-PVB-5533S0100-736X20180008015702-s2.0-85056104182S0100-736X2018000801570.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPporPesquisa Veterinaria Brasileirainfo:eu-repo/semantics/openAccess2023-11-04T06:10:17Zoai:repositorio.unesp.br:11449/187035Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462023-11-04T06:10:17Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Hepatic steatosis alters cellular recruitment during induced aerocystitis in Nile tilapia Esteatose hepática altera acúmulo celular em tilápias do Nilo durante aerocistite infecciosa |
title |
Hepatic steatosis alters cellular recruitment during induced aerocystitis in Nile tilapia |
spellingShingle |
Hepatic steatosis alters cellular recruitment during induced aerocystitis in Nile tilapia Moraes, Alessandra C. [UNESP] Acute inflammation Aerocystitis Carbon tetrachloride Cichlid fish Glucocorticoid Hepatic steatosis Tilapia |
title_short |
Hepatic steatosis alters cellular recruitment during induced aerocystitis in Nile tilapia |
title_full |
Hepatic steatosis alters cellular recruitment during induced aerocystitis in Nile tilapia |
title_fullStr |
Hepatic steatosis alters cellular recruitment during induced aerocystitis in Nile tilapia |
title_full_unstemmed |
Hepatic steatosis alters cellular recruitment during induced aerocystitis in Nile tilapia |
title_sort |
Hepatic steatosis alters cellular recruitment during induced aerocystitis in Nile tilapia |
author |
Moraes, Alessandra C. [UNESP] |
author_facet |
Moraes, Alessandra C. [UNESP] Prado, Ed J.R. [UNESP] Foz, Elizabeth P. Barbuio, Roberto [UNESP] Faria, Vanessa P. Belo, Marco A.A. [UNESP] |
author_role |
author |
author2 |
Prado, Ed J.R. [UNESP] Foz, Elizabeth P. Barbuio, Roberto [UNESP] Faria, Vanessa P. Belo, Marco A.A. [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Médica Veterinária Autônoma Laboratório de Patologia Clínica Max Vet Hospital Veterinário |
dc.contributor.author.fl_str_mv |
Moraes, Alessandra C. [UNESP] Prado, Ed J.R. [UNESP] Foz, Elizabeth P. Barbuio, Roberto [UNESP] Faria, Vanessa P. Belo, Marco A.A. [UNESP] |
dc.subject.por.fl_str_mv |
Acute inflammation Aerocystitis Carbon tetrachloride Cichlid fish Glucocorticoid Hepatic steatosis Tilapia |
topic |
Acute inflammation Aerocystitis Carbon tetrachloride Cichlid fish Glucocorticoid Hepatic steatosis Tilapia |
description |
The study evaluated the hepatotoxicity induced by CCl4 during the glucocorticoid effect of dexamethasone (DEX) on the pathophysiology of the acute inflammatory reaction in Nile tilapia, Oreochromis niloticus, correlating hepatic functionality with cellular accumulation kinetics in infectious aerocystitis. Eighty- four Nile tilapia were distributed into four treatments: control, CCl4, DEX and CCl4 + DEX. Seven tilapia were sampled per treatment in three periods: 6, 24 and 48h after induction of inflammation. CCl4 was used in a single dose of 0.5mL/kg intraperitoneally to cause hepatic disorder. Aeromonas hydrophila inoculum was used to induce aerocystitis. Dexamethasone was administered intramuscularly at the dose of 2mg/kg b. w. The results revealed a higher serum aspartate transaminase (AST) activity associated with greater somatic liver alteration, being these findings inversely proportional to the cellular accumulation in the inflammatory focus, demonstrating a lower number of inflammatory cells in the animals affected with a higher degree of hepatic disorders induced by CCl4. The histopathological study revealed transient degenerative changes in the most acute phase, as livers of tilapia showed accumulation of lipids in hepatocytes 6 hours after administration of CCl4, and this fatty degeneration was no longer observed in 24 and 48h. However, administration of CCl4 in Nile tilapia resulted in acute and transient liver degeneration, characterized by accumulation of fat in hepatocytes, increased AST in the blood and hepatomegaly. With liver dysfunction there was compromise of cellular recruitment in infectious aerocystitis, indicating that there is liver involvement in the innate immune response in tilapia. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-08-01 2019-10-06T15:23:30Z 2019-10-06T15:23:30Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1590/1678-5150-PVB-5533 Pesquisa Veterinaria Brasileira, v. 38, n. 8, p. 1570-1576, 2018. 1678-5150 0100-736X http://hdl.handle.net/11449/187035 10.1590/1678-5150-PVB-5533 S0100-736X2018000801570 2-s2.0-85056104182 S0100-736X2018000801570.pdf |
url |
http://dx.doi.org/10.1590/1678-5150-PVB-5533 http://hdl.handle.net/11449/187035 |
identifier_str_mv |
Pesquisa Veterinaria Brasileira, v. 38, n. 8, p. 1570-1576, 2018. 1678-5150 0100-736X 10.1590/1678-5150-PVB-5533 S0100-736X2018000801570 2-s2.0-85056104182 S0100-736X2018000801570.pdf |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
Pesquisa Veterinaria Brasileira |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1570-1576 application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1792961734075482112 |