Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus

Detalhes bibliográficos
Autor(a) principal: Gimenes, R. [UNESP]
Data de Publicação: 2018
Outros Autores: Gimenes, C., Rosa, C. M. [UNESP], Xavier, N. P. [UNESP], Campos, D. H.S. [UNESP], Fernandes, A. A.H. [UNESP], Cezar, M. D.M. [UNESP], Guirado, G. N. [UNESP], Pagan, L. U. [UNESP], Chaer, I. D. [UNESP], Fernandes, D. C., Laurindo, F. R., Cicogna, A. C. [UNESP], Okoshi, M. P. [UNESP], Okoshi, K. [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1186/s12933-017-0657-9
http://hdl.handle.net/11449/175757
Resumo: Background: Increased reactive oxygen species (ROS) generation in diabetes mellitus (DM) is an important mechanism leading to diabetic cardiomyopathy. Apocynin, a drug isolated from the herb Picrorhiza kurroa, is considered an antioxidant agent by inhibiting NADPH oxidase activity and improving ROS scavenging. This study analyzed the influence of apocynin on cardiac remodeling in diabetic rats. Methods: Six-month-old male Wistar rats were assigned into 4 groups: control (CTL, n=15), control+apocynin (CTL+APO, n=20), diabetes (DM, n=20), and diabetes+apocynin (DM+APO, n=20). DM was induced by streptozotocin. Seven days later, apocynin (16mg/kg/day) or vehicle was initiated and maintained for 8weeks. Left ventricular (LV) histological sections were used to analyze interstitial collagen fraction. NADPH oxidase activity was evaluated in LV samples. Comparisons between groups were performed by ANOVA for a 2×2 factorial design followed by the Bonferroni post hoc test. Results: Body weight (BW) was lower and glycemia higher in diabetic animals. Echocardiogram showed increased left atrial diameter, LV diastolic diameter, and LV mass indexed by BW in both diabetic groups; apocynin did not affect these indices. LV systolic function was impaired in DM groups and unchanged by apocynin. Isovolumic relaxation time was increased in DM groups; transmitral E/A ratio was higher in DM+APO compared to DM. Myocardial functional evaluation through papillary muscle preparations showed impaired contractile and relaxation function in both DM groups at baseline conditions. After positive inotropic stimulation, developed tension (DT) was lower in DM than CTL. In DM+APO, DT had values between those in DM and CTL+APO and did not significantly differ from either group. Myocardial interstitial collagen fraction was higher in DM than CTL and did not differ between DM+APO and CTL+APO. Serum activity of antioxidant enzymes glutathione peroxidase, superoxide dismutase (SOD), and catalase was lower in DM than CTL; apocynin restored catalase and SOD levels in DM+APO. Myocardial NADPH oxidase activity did not differ between groups. Conclusion: Apocynin restores serum antioxidant enzyme activity despite unchanged myocardial NADPH oxidase activity in diabetic rats.
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spelling Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitusDiabetic cardiomyopathyEchocardiogramHeart failureMyocardial functionNADPH oxidaseNADPH oxidase blockerOxidative stressPapillary muscleRatVentricular functionBackground: Increased reactive oxygen species (ROS) generation in diabetes mellitus (DM) is an important mechanism leading to diabetic cardiomyopathy. Apocynin, a drug isolated from the herb Picrorhiza kurroa, is considered an antioxidant agent by inhibiting NADPH oxidase activity and improving ROS scavenging. This study analyzed the influence of apocynin on cardiac remodeling in diabetic rats. Methods: Six-month-old male Wistar rats were assigned into 4 groups: control (CTL, n=15), control+apocynin (CTL+APO, n=20), diabetes (DM, n=20), and diabetes+apocynin (DM+APO, n=20). DM was induced by streptozotocin. Seven days later, apocynin (16mg/kg/day) or vehicle was initiated and maintained for 8weeks. Left ventricular (LV) histological sections were used to analyze interstitial collagen fraction. NADPH oxidase activity was evaluated in LV samples. Comparisons between groups were performed by ANOVA for a 2×2 factorial design followed by the Bonferroni post hoc test. Results: Body weight (BW) was lower and glycemia higher in diabetic animals. Echocardiogram showed increased left atrial diameter, LV diastolic diameter, and LV mass indexed by BW in both diabetic groups; apocynin did not affect these indices. LV systolic function was impaired in DM groups and unchanged by apocynin. Isovolumic relaxation time was increased in DM groups; transmitral E/A ratio was higher in DM+APO compared to DM. Myocardial functional evaluation through papillary muscle preparations showed impaired contractile and relaxation function in both DM groups at baseline conditions. After positive inotropic stimulation, developed tension (DT) was lower in DM than CTL. In DM+APO, DT had values between those in DM and CTL+APO and did not significantly differ from either group. Myocardial interstitial collagen fraction was higher in DM than CTL and did not differ between DM+APO and CTL+APO. Serum activity of antioxidant enzymes glutathione peroxidase, superoxide dismutase (SOD), and catalase was lower in DM than CTL; apocynin restored catalase and SOD levels in DM+APO. Myocardial NADPH oxidase activity did not differ between groups. Conclusion: Apocynin restores serum antioxidant enzyme activity despite unchanged myocardial NADPH oxidase activity in diabetic rats.Sao Paulo State University UNESP Department of Internal Medicine Botucatu Medical SchoolSagrado Coração UniversitySao Paulo State University (UNESP) Institute of BiosciencesSao Paulo University USP Department of Cardiopneumology Medical SchoolSao Paulo State University UNESP Departamento de Clinica Medica Faculdade de Medicina de Botucatu, Rubiao Junior, S/NSao Paulo State University UNESP Department of Internal Medicine Botucatu Medical SchoolSao Paulo State University (UNESP) Institute of BiosciencesSao Paulo State University UNESP Departamento de Clinica Medica Faculdade de Medicina de Botucatu, Rubiao Junior, S/NUniversidade Estadual Paulista (Unesp)Sagrado Coração UniversityUniversidade de São Paulo (USP)Gimenes, R. [UNESP]Gimenes, C.Rosa, C. M. [UNESP]Xavier, N. P. [UNESP]Campos, D. H.S. [UNESP]Fernandes, A. A.H. [UNESP]Cezar, M. D.M. [UNESP]Guirado, G. N. [UNESP]Pagan, L. U. [UNESP]Chaer, I. D. [UNESP]Fernandes, D. C.Laurindo, F. R.Cicogna, A. C. [UNESP]Okoshi, M. P. [UNESP]Okoshi, K. [UNESP]2018-12-11T17:17:23Z2018-12-11T17:17:23Z2018-01-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s12933-017-0657-9Cardiovascular Diabetology, v. 17, n. 1, 2018.1475-2840http://hdl.handle.net/11449/17575710.1186/s12933-017-0657-92-s2.0-850407161932-s2.0-85040716193.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCardiovascular Diabetology2,157info:eu-repo/semantics/openAccess2024-08-14T17:36:54Zoai:repositorio.unesp.br:11449/175757Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:36:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus
title Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus
spellingShingle Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus
Gimenes, R. [UNESP]
Diabetic cardiomyopathy
Echocardiogram
Heart failure
Myocardial function
NADPH oxidase
NADPH oxidase blocker
Oxidative stress
Papillary muscle
Rat
Ventricular function
title_short Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus
title_full Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus
title_fullStr Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus
title_full_unstemmed Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus
title_sort Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus
author Gimenes, R. [UNESP]
author_facet Gimenes, R. [UNESP]
Gimenes, C.
Rosa, C. M. [UNESP]
Xavier, N. P. [UNESP]
Campos, D. H.S. [UNESP]
Fernandes, A. A.H. [UNESP]
Cezar, M. D.M. [UNESP]
Guirado, G. N. [UNESP]
Pagan, L. U. [UNESP]
Chaer, I. D. [UNESP]
Fernandes, D. C.
Laurindo, F. R.
Cicogna, A. C. [UNESP]
Okoshi, M. P. [UNESP]
Okoshi, K. [UNESP]
author_role author
author2 Gimenes, C.
Rosa, C. M. [UNESP]
Xavier, N. P. [UNESP]
Campos, D. H.S. [UNESP]
Fernandes, A. A.H. [UNESP]
Cezar, M. D.M. [UNESP]
Guirado, G. N. [UNESP]
Pagan, L. U. [UNESP]
Chaer, I. D. [UNESP]
Fernandes, D. C.
Laurindo, F. R.
Cicogna, A. C. [UNESP]
Okoshi, M. P. [UNESP]
Okoshi, K. [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Sagrado Coração University
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Gimenes, R. [UNESP]
Gimenes, C.
Rosa, C. M. [UNESP]
Xavier, N. P. [UNESP]
Campos, D. H.S. [UNESP]
Fernandes, A. A.H. [UNESP]
Cezar, M. D.M. [UNESP]
Guirado, G. N. [UNESP]
Pagan, L. U. [UNESP]
Chaer, I. D. [UNESP]
Fernandes, D. C.
Laurindo, F. R.
Cicogna, A. C. [UNESP]
Okoshi, M. P. [UNESP]
Okoshi, K. [UNESP]
dc.subject.por.fl_str_mv Diabetic cardiomyopathy
Echocardiogram
Heart failure
Myocardial function
NADPH oxidase
NADPH oxidase blocker
Oxidative stress
Papillary muscle
Rat
Ventricular function
topic Diabetic cardiomyopathy
Echocardiogram
Heart failure
Myocardial function
NADPH oxidase
NADPH oxidase blocker
Oxidative stress
Papillary muscle
Rat
Ventricular function
description Background: Increased reactive oxygen species (ROS) generation in diabetes mellitus (DM) is an important mechanism leading to diabetic cardiomyopathy. Apocynin, a drug isolated from the herb Picrorhiza kurroa, is considered an antioxidant agent by inhibiting NADPH oxidase activity and improving ROS scavenging. This study analyzed the influence of apocynin on cardiac remodeling in diabetic rats. Methods: Six-month-old male Wistar rats were assigned into 4 groups: control (CTL, n=15), control+apocynin (CTL+APO, n=20), diabetes (DM, n=20), and diabetes+apocynin (DM+APO, n=20). DM was induced by streptozotocin. Seven days later, apocynin (16mg/kg/day) or vehicle was initiated and maintained for 8weeks. Left ventricular (LV) histological sections were used to analyze interstitial collagen fraction. NADPH oxidase activity was evaluated in LV samples. Comparisons between groups were performed by ANOVA for a 2×2 factorial design followed by the Bonferroni post hoc test. Results: Body weight (BW) was lower and glycemia higher in diabetic animals. Echocardiogram showed increased left atrial diameter, LV diastolic diameter, and LV mass indexed by BW in both diabetic groups; apocynin did not affect these indices. LV systolic function was impaired in DM groups and unchanged by apocynin. Isovolumic relaxation time was increased in DM groups; transmitral E/A ratio was higher in DM+APO compared to DM. Myocardial functional evaluation through papillary muscle preparations showed impaired contractile and relaxation function in both DM groups at baseline conditions. After positive inotropic stimulation, developed tension (DT) was lower in DM than CTL. In DM+APO, DT had values between those in DM and CTL+APO and did not significantly differ from either group. Myocardial interstitial collagen fraction was higher in DM than CTL and did not differ between DM+APO and CTL+APO. Serum activity of antioxidant enzymes glutathione peroxidase, superoxide dismutase (SOD), and catalase was lower in DM than CTL; apocynin restored catalase and SOD levels in DM+APO. Myocardial NADPH oxidase activity did not differ between groups. Conclusion: Apocynin restores serum antioxidant enzyme activity despite unchanged myocardial NADPH oxidase activity in diabetic rats.
publishDate 2018
dc.date.none.fl_str_mv 2018-12-11T17:17:23Z
2018-12-11T17:17:23Z
2018-01-17
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1186/s12933-017-0657-9
Cardiovascular Diabetology, v. 17, n. 1, 2018.
1475-2840
http://hdl.handle.net/11449/175757
10.1186/s12933-017-0657-9
2-s2.0-85040716193
2-s2.0-85040716193.pdf
url http://dx.doi.org/10.1186/s12933-017-0657-9
http://hdl.handle.net/11449/175757
identifier_str_mv Cardiovascular Diabetology, v. 17, n. 1, 2018.
1475-2840
10.1186/s12933-017-0657-9
2-s2.0-85040716193
2-s2.0-85040716193.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Cardiovascular Diabetology
2,157
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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