Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1186/s12933-017-0657-9 http://hdl.handle.net/11449/175757 |
Resumo: | Background: Increased reactive oxygen species (ROS) generation in diabetes mellitus (DM) is an important mechanism leading to diabetic cardiomyopathy. Apocynin, a drug isolated from the herb Picrorhiza kurroa, is considered an antioxidant agent by inhibiting NADPH oxidase activity and improving ROS scavenging. This study analyzed the influence of apocynin on cardiac remodeling in diabetic rats. Methods: Six-month-old male Wistar rats were assigned into 4 groups: control (CTL, n=15), control+apocynin (CTL+APO, n=20), diabetes (DM, n=20), and diabetes+apocynin (DM+APO, n=20). DM was induced by streptozotocin. Seven days later, apocynin (16mg/kg/day) or vehicle was initiated and maintained for 8weeks. Left ventricular (LV) histological sections were used to analyze interstitial collagen fraction. NADPH oxidase activity was evaluated in LV samples. Comparisons between groups were performed by ANOVA for a 2×2 factorial design followed by the Bonferroni post hoc test. Results: Body weight (BW) was lower and glycemia higher in diabetic animals. Echocardiogram showed increased left atrial diameter, LV diastolic diameter, and LV mass indexed by BW in both diabetic groups; apocynin did not affect these indices. LV systolic function was impaired in DM groups and unchanged by apocynin. Isovolumic relaxation time was increased in DM groups; transmitral E/A ratio was higher in DM+APO compared to DM. Myocardial functional evaluation through papillary muscle preparations showed impaired contractile and relaxation function in both DM groups at baseline conditions. After positive inotropic stimulation, developed tension (DT) was lower in DM than CTL. In DM+APO, DT had values between those in DM and CTL+APO and did not significantly differ from either group. Myocardial interstitial collagen fraction was higher in DM than CTL and did not differ between DM+APO and CTL+APO. Serum activity of antioxidant enzymes glutathione peroxidase, superoxide dismutase (SOD), and catalase was lower in DM than CTL; apocynin restored catalase and SOD levels in DM+APO. Myocardial NADPH oxidase activity did not differ between groups. Conclusion: Apocynin restores serum antioxidant enzyme activity despite unchanged myocardial NADPH oxidase activity in diabetic rats. |
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Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitusDiabetic cardiomyopathyEchocardiogramHeart failureMyocardial functionNADPH oxidaseNADPH oxidase blockerOxidative stressPapillary muscleRatVentricular functionBackground: Increased reactive oxygen species (ROS) generation in diabetes mellitus (DM) is an important mechanism leading to diabetic cardiomyopathy. Apocynin, a drug isolated from the herb Picrorhiza kurroa, is considered an antioxidant agent by inhibiting NADPH oxidase activity and improving ROS scavenging. This study analyzed the influence of apocynin on cardiac remodeling in diabetic rats. Methods: Six-month-old male Wistar rats were assigned into 4 groups: control (CTL, n=15), control+apocynin (CTL+APO, n=20), diabetes (DM, n=20), and diabetes+apocynin (DM+APO, n=20). DM was induced by streptozotocin. Seven days later, apocynin (16mg/kg/day) or vehicle was initiated and maintained for 8weeks. Left ventricular (LV) histological sections were used to analyze interstitial collagen fraction. NADPH oxidase activity was evaluated in LV samples. Comparisons between groups were performed by ANOVA for a 2×2 factorial design followed by the Bonferroni post hoc test. Results: Body weight (BW) was lower and glycemia higher in diabetic animals. Echocardiogram showed increased left atrial diameter, LV diastolic diameter, and LV mass indexed by BW in both diabetic groups; apocynin did not affect these indices. LV systolic function was impaired in DM groups and unchanged by apocynin. Isovolumic relaxation time was increased in DM groups; transmitral E/A ratio was higher in DM+APO compared to DM. Myocardial functional evaluation through papillary muscle preparations showed impaired contractile and relaxation function in both DM groups at baseline conditions. After positive inotropic stimulation, developed tension (DT) was lower in DM than CTL. In DM+APO, DT had values between those in DM and CTL+APO and did not significantly differ from either group. Myocardial interstitial collagen fraction was higher in DM than CTL and did not differ between DM+APO and CTL+APO. Serum activity of antioxidant enzymes glutathione peroxidase, superoxide dismutase (SOD), and catalase was lower in DM than CTL; apocynin restored catalase and SOD levels in DM+APO. Myocardial NADPH oxidase activity did not differ between groups. Conclusion: Apocynin restores serum antioxidant enzyme activity despite unchanged myocardial NADPH oxidase activity in diabetic rats.Sao Paulo State University UNESP Department of Internal Medicine Botucatu Medical SchoolSagrado Coração UniversitySao Paulo State University (UNESP) Institute of BiosciencesSao Paulo University USP Department of Cardiopneumology Medical SchoolSao Paulo State University UNESP Departamento de Clinica Medica Faculdade de Medicina de Botucatu, Rubiao Junior, S/NSao Paulo State University UNESP Department of Internal Medicine Botucatu Medical SchoolSao Paulo State University (UNESP) Institute of BiosciencesSao Paulo State University UNESP Departamento de Clinica Medica Faculdade de Medicina de Botucatu, Rubiao Junior, S/NUniversidade Estadual Paulista (Unesp)Sagrado Coração UniversityUniversidade de São Paulo (USP)Gimenes, R. [UNESP]Gimenes, C.Rosa, C. M. [UNESP]Xavier, N. P. [UNESP]Campos, D. H.S. [UNESP]Fernandes, A. A.H. [UNESP]Cezar, M. D.M. [UNESP]Guirado, G. N. [UNESP]Pagan, L. U. [UNESP]Chaer, I. D. [UNESP]Fernandes, D. C.Laurindo, F. R.Cicogna, A. C. [UNESP]Okoshi, M. P. [UNESP]Okoshi, K. [UNESP]2018-12-11T17:17:23Z2018-12-11T17:17:23Z2018-01-17info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleapplication/pdfhttp://dx.doi.org/10.1186/s12933-017-0657-9Cardiovascular Diabetology, v. 17, n. 1, 2018.1475-2840http://hdl.handle.net/11449/17575710.1186/s12933-017-0657-92-s2.0-850407161932-s2.0-85040716193.pdfScopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengCardiovascular Diabetology2,157info:eu-repo/semantics/openAccess2024-08-14T17:36:54Zoai:repositorio.unesp.br:11449/175757Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:36:54Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus |
title |
Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus |
spellingShingle |
Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus Gimenes, R. [UNESP] Diabetic cardiomyopathy Echocardiogram Heart failure Myocardial function NADPH oxidase NADPH oxidase blocker Oxidative stress Papillary muscle Rat Ventricular function |
title_short |
Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus |
title_full |
Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus |
title_fullStr |
Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus |
title_full_unstemmed |
Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus |
title_sort |
Influence of apocynin on cardiac remodeling in rats with streptozotocin-induced diabetes mellitus |
author |
Gimenes, R. [UNESP] |
author_facet |
Gimenes, R. [UNESP] Gimenes, C. Rosa, C. M. [UNESP] Xavier, N. P. [UNESP] Campos, D. H.S. [UNESP] Fernandes, A. A.H. [UNESP] Cezar, M. D.M. [UNESP] Guirado, G. N. [UNESP] Pagan, L. U. [UNESP] Chaer, I. D. [UNESP] Fernandes, D. C. Laurindo, F. R. Cicogna, A. C. [UNESP] Okoshi, M. P. [UNESP] Okoshi, K. [UNESP] |
author_role |
author |
author2 |
Gimenes, C. Rosa, C. M. [UNESP] Xavier, N. P. [UNESP] Campos, D. H.S. [UNESP] Fernandes, A. A.H. [UNESP] Cezar, M. D.M. [UNESP] Guirado, G. N. [UNESP] Pagan, L. U. [UNESP] Chaer, I. D. [UNESP] Fernandes, D. C. Laurindo, F. R. Cicogna, A. C. [UNESP] Okoshi, M. P. [UNESP] Okoshi, K. [UNESP] |
author2_role |
author author author author author author author author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) Sagrado Coração University Universidade de São Paulo (USP) |
dc.contributor.author.fl_str_mv |
Gimenes, R. [UNESP] Gimenes, C. Rosa, C. M. [UNESP] Xavier, N. P. [UNESP] Campos, D. H.S. [UNESP] Fernandes, A. A.H. [UNESP] Cezar, M. D.M. [UNESP] Guirado, G. N. [UNESP] Pagan, L. U. [UNESP] Chaer, I. D. [UNESP] Fernandes, D. C. Laurindo, F. R. Cicogna, A. C. [UNESP] Okoshi, M. P. [UNESP] Okoshi, K. [UNESP] |
dc.subject.por.fl_str_mv |
Diabetic cardiomyopathy Echocardiogram Heart failure Myocardial function NADPH oxidase NADPH oxidase blocker Oxidative stress Papillary muscle Rat Ventricular function |
topic |
Diabetic cardiomyopathy Echocardiogram Heart failure Myocardial function NADPH oxidase NADPH oxidase blocker Oxidative stress Papillary muscle Rat Ventricular function |
description |
Background: Increased reactive oxygen species (ROS) generation in diabetes mellitus (DM) is an important mechanism leading to diabetic cardiomyopathy. Apocynin, a drug isolated from the herb Picrorhiza kurroa, is considered an antioxidant agent by inhibiting NADPH oxidase activity and improving ROS scavenging. This study analyzed the influence of apocynin on cardiac remodeling in diabetic rats. Methods: Six-month-old male Wistar rats were assigned into 4 groups: control (CTL, n=15), control+apocynin (CTL+APO, n=20), diabetes (DM, n=20), and diabetes+apocynin (DM+APO, n=20). DM was induced by streptozotocin. Seven days later, apocynin (16mg/kg/day) or vehicle was initiated and maintained for 8weeks. Left ventricular (LV) histological sections were used to analyze interstitial collagen fraction. NADPH oxidase activity was evaluated in LV samples. Comparisons between groups were performed by ANOVA for a 2×2 factorial design followed by the Bonferroni post hoc test. Results: Body weight (BW) was lower and glycemia higher in diabetic animals. Echocardiogram showed increased left atrial diameter, LV diastolic diameter, and LV mass indexed by BW in both diabetic groups; apocynin did not affect these indices. LV systolic function was impaired in DM groups and unchanged by apocynin. Isovolumic relaxation time was increased in DM groups; transmitral E/A ratio was higher in DM+APO compared to DM. Myocardial functional evaluation through papillary muscle preparations showed impaired contractile and relaxation function in both DM groups at baseline conditions. After positive inotropic stimulation, developed tension (DT) was lower in DM than CTL. In DM+APO, DT had values between those in DM and CTL+APO and did not significantly differ from either group. Myocardial interstitial collagen fraction was higher in DM than CTL and did not differ between DM+APO and CTL+APO. Serum activity of antioxidant enzymes glutathione peroxidase, superoxide dismutase (SOD), and catalase was lower in DM than CTL; apocynin restored catalase and SOD levels in DM+APO. Myocardial NADPH oxidase activity did not differ between groups. Conclusion: Apocynin restores serum antioxidant enzyme activity despite unchanged myocardial NADPH oxidase activity in diabetic rats. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-11T17:17:23Z 2018-12-11T17:17:23Z 2018-01-17 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1186/s12933-017-0657-9 Cardiovascular Diabetology, v. 17, n. 1, 2018. 1475-2840 http://hdl.handle.net/11449/175757 10.1186/s12933-017-0657-9 2-s2.0-85040716193 2-s2.0-85040716193.pdf |
url |
http://dx.doi.org/10.1186/s12933-017-0657-9 http://hdl.handle.net/11449/175757 |
identifier_str_mv |
Cardiovascular Diabetology, v. 17, n. 1, 2018. 1475-2840 10.1186/s12933-017-0657-9 2-s2.0-85040716193 2-s2.0-85040716193.pdf |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Cardiovascular Diabetology 2,157 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
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1808128208447471616 |