Influence of Isolated Resistance Exercise on Cardiac Remodeling, Myocardial Oxidative Stress, and Metabolism in Infarcted Rats

Detalhes bibliográficos
Autor(a) principal: Rodrigues, Eder Anderson [UNESP]
Data de Publicação: 2023
Outros Autores: Lima, Aline Regina Ruiz [UNESP], Gomes, Mariana Janini, Souza, Lidiane Moreira [UNESP], Pontes, Thierres Hernani Dias [UNESP], Pagan, Luana Urbano [UNESP], Murata, Gilson Masahiro, Damatto, Felipe Cesar [UNESP], Carvalho Depra, Igor [UNESP], Rego, Amanda Bergamo Gonçalves Castro [UNESP], Reyes, David Rafael Abreu [UNESP], Zornoff, Leonardo Antonio Mamede [UNESP], Okoshi, Katashi [UNESP], Okoshi, Marina Politi [UNESP]
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.3390/antiox12040896
http://hdl.handle.net/11449/248768
Resumo: Introduction: Exercise is an important therapeutic strategy for preventing and treating myocardial infarction (MI)-induced cardiac remodeling and heart failure. However, the myocardial effects of resistance exercise on infarcted hearts are not completely established. In this study, we investigated the effects of resistance exercise on structural, functional, and molecular cardiac alterations in infarcted rats. Methods: Three months after MI induction or simulated surgery, Wistar rats were assigned into three groups: Sham (n = 14); MI (n = 9); and exercised MI (MI-Ex, n = 13). Exercised rats performed, 3 times a week for 12 weeks, four climbs on a ladder with progressive loads. Cardiac structure and left ventricle (LV) function were analyzed by echocardiogram. Myocyte diameters were evaluated in hematoxylin- and eosin-stained histological sections as the smallest distance between borders drawn across the nucleus. Myocardial energy metabolism, lipid hydroperoxide, malondialdehyde, protein carbonylation, and antioxidant enzyme activities were evaluated by spectrophotometry. Gene expressions of NADPH oxidase subunits were evaluated by RT-PCR. Statistical analyses were performed using ANOVA and Tukey or Kruskal–Wallis and Dunn’s test. Results: Mortality did not differ between the MI-Ex and MI groups. MI had dilated left atrium and LV, with LV systolic dysfunction. Exercise increased the maximum load-carrying capacity, with no changes in cardiac structure or LV function. Myocyte diameters were lower in MI than in Sham and MI-Ex. Lactate dehydrogenase and creatine kinase activity were lower in MI than in Sham. Citrate synthase and catalase activity were lower in MI and MI-Ex than in Sham. Lipid hydroperoxide concentration was lower in MI-Ex than in MI. Nox2 and p22phox gene expressions were higher in MI-Ex than in Sham. Gene expression of Nox4 was higher in MI and MI-Ex than in Sham, and p47phox was lower in MI than in Sham. Conclusion: Late resistance exercise was safe in infarcted rats. Resistance exercise improved maximum load-carrying capacity, reduced myocardial oxidative stress, and preserved myocardial metabolism, with no changes in cardiac structure or left ventricle function in infarcted rats.
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spelling Influence of Isolated Resistance Exercise on Cardiac Remodeling, Myocardial Oxidative Stress, and Metabolism in Infarcted Ratsmetabolism enzymesmyocardial infarctionNADPH oxidasephysical exerciseratventricular functionIntroduction: Exercise is an important therapeutic strategy for preventing and treating myocardial infarction (MI)-induced cardiac remodeling and heart failure. However, the myocardial effects of resistance exercise on infarcted hearts are not completely established. In this study, we investigated the effects of resistance exercise on structural, functional, and molecular cardiac alterations in infarcted rats. Methods: Three months after MI induction or simulated surgery, Wistar rats were assigned into three groups: Sham (n = 14); MI (n = 9); and exercised MI (MI-Ex, n = 13). Exercised rats performed, 3 times a week for 12 weeks, four climbs on a ladder with progressive loads. Cardiac structure and left ventricle (LV) function were analyzed by echocardiogram. Myocyte diameters were evaluated in hematoxylin- and eosin-stained histological sections as the smallest distance between borders drawn across the nucleus. Myocardial energy metabolism, lipid hydroperoxide, malondialdehyde, protein carbonylation, and antioxidant enzyme activities were evaluated by spectrophotometry. Gene expressions of NADPH oxidase subunits were evaluated by RT-PCR. Statistical analyses were performed using ANOVA and Tukey or Kruskal–Wallis and Dunn’s test. Results: Mortality did not differ between the MI-Ex and MI groups. MI had dilated left atrium and LV, with LV systolic dysfunction. Exercise increased the maximum load-carrying capacity, with no changes in cardiac structure or LV function. Myocyte diameters were lower in MI than in Sham and MI-Ex. Lactate dehydrogenase and creatine kinase activity were lower in MI than in Sham. Citrate synthase and catalase activity were lower in MI and MI-Ex than in Sham. Lipid hydroperoxide concentration was lower in MI-Ex than in MI. Nox2 and p22phox gene expressions were higher in MI-Ex than in Sham. Gene expression of Nox4 was higher in MI and MI-Ex than in Sham, and p47phox was lower in MI than in Sham. Conclusion: Late resistance exercise was safe in infarcted rats. Resistance exercise improved maximum load-carrying capacity, reduced myocardial oxidative stress, and preserved myocardial metabolism, with no changes in cardiac structure or left ventricle function in infarcted rats.Universidade Estadual PaulistaCoordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Department of Internal Medicine Botucatu Medical School Sao Paulo State University (UNESP), SPDepartment of Kinesiology and Sport Management Texas A&M UniversityLIM29 Division of Nephrology University of Sao Paulo Medical School, SPDepartment of Internal Medicine Botucatu Medical School Sao Paulo State University (UNESP), SPCAPES: 001CNPq: 140400/2019-2FAPESP: 2021/10923-5CNPq: 307280/2022-5CNPq: 307703/2022-3Universidade Estadual Paulista (UNESP)Texas A&M UniversityUniversidade de São Paulo (USP)Rodrigues, Eder Anderson [UNESP]Lima, Aline Regina Ruiz [UNESP]Gomes, Mariana JaniniSouza, Lidiane Moreira [UNESP]Pontes, Thierres Hernani Dias [UNESP]Pagan, Luana Urbano [UNESP]Murata, Gilson MasahiroDamatto, Felipe Cesar [UNESP]Carvalho Depra, Igor [UNESP]Rego, Amanda Bergamo Gonçalves Castro [UNESP]Reyes, David Rafael Abreu [UNESP]Zornoff, Leonardo Antonio Mamede [UNESP]Okoshi, Katashi [UNESP]Okoshi, Marina Politi [UNESP]2023-07-29T13:53:10Z2023-07-29T13:53:10Z2023-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/antiox12040896Antioxidants, v. 12, n. 4, 2023.2076-3921http://hdl.handle.net/11449/24876810.3390/antiox120408962-s2.0-85156199692Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengAntioxidantsinfo:eu-repo/semantics/openAccess2024-08-14T17:23:32Zoai:repositorio.unesp.br:11449/248768Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-14T17:23:32Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Influence of Isolated Resistance Exercise on Cardiac Remodeling, Myocardial Oxidative Stress, and Metabolism in Infarcted Rats
title Influence of Isolated Resistance Exercise on Cardiac Remodeling, Myocardial Oxidative Stress, and Metabolism in Infarcted Rats
spellingShingle Influence of Isolated Resistance Exercise on Cardiac Remodeling, Myocardial Oxidative Stress, and Metabolism in Infarcted Rats
Rodrigues, Eder Anderson [UNESP]
metabolism enzymes
myocardial infarction
NADPH oxidase
physical exercise
rat
ventricular function
title_short Influence of Isolated Resistance Exercise on Cardiac Remodeling, Myocardial Oxidative Stress, and Metabolism in Infarcted Rats
title_full Influence of Isolated Resistance Exercise on Cardiac Remodeling, Myocardial Oxidative Stress, and Metabolism in Infarcted Rats
title_fullStr Influence of Isolated Resistance Exercise on Cardiac Remodeling, Myocardial Oxidative Stress, and Metabolism in Infarcted Rats
title_full_unstemmed Influence of Isolated Resistance Exercise on Cardiac Remodeling, Myocardial Oxidative Stress, and Metabolism in Infarcted Rats
title_sort Influence of Isolated Resistance Exercise on Cardiac Remodeling, Myocardial Oxidative Stress, and Metabolism in Infarcted Rats
author Rodrigues, Eder Anderson [UNESP]
author_facet Rodrigues, Eder Anderson [UNESP]
Lima, Aline Regina Ruiz [UNESP]
Gomes, Mariana Janini
Souza, Lidiane Moreira [UNESP]
Pontes, Thierres Hernani Dias [UNESP]
Pagan, Luana Urbano [UNESP]
Murata, Gilson Masahiro
Damatto, Felipe Cesar [UNESP]
Carvalho Depra, Igor [UNESP]
Rego, Amanda Bergamo Gonçalves Castro [UNESP]
Reyes, David Rafael Abreu [UNESP]
Zornoff, Leonardo Antonio Mamede [UNESP]
Okoshi, Katashi [UNESP]
Okoshi, Marina Politi [UNESP]
author_role author
author2 Lima, Aline Regina Ruiz [UNESP]
Gomes, Mariana Janini
Souza, Lidiane Moreira [UNESP]
Pontes, Thierres Hernani Dias [UNESP]
Pagan, Luana Urbano [UNESP]
Murata, Gilson Masahiro
Damatto, Felipe Cesar [UNESP]
Carvalho Depra, Igor [UNESP]
Rego, Amanda Bergamo Gonçalves Castro [UNESP]
Reyes, David Rafael Abreu [UNESP]
Zornoff, Leonardo Antonio Mamede [UNESP]
Okoshi, Katashi [UNESP]
Okoshi, Marina Politi [UNESP]
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (UNESP)
Texas A&M University
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Rodrigues, Eder Anderson [UNESP]
Lima, Aline Regina Ruiz [UNESP]
Gomes, Mariana Janini
Souza, Lidiane Moreira [UNESP]
Pontes, Thierres Hernani Dias [UNESP]
Pagan, Luana Urbano [UNESP]
Murata, Gilson Masahiro
Damatto, Felipe Cesar [UNESP]
Carvalho Depra, Igor [UNESP]
Rego, Amanda Bergamo Gonçalves Castro [UNESP]
Reyes, David Rafael Abreu [UNESP]
Zornoff, Leonardo Antonio Mamede [UNESP]
Okoshi, Katashi [UNESP]
Okoshi, Marina Politi [UNESP]
dc.subject.por.fl_str_mv metabolism enzymes
myocardial infarction
NADPH oxidase
physical exercise
rat
ventricular function
topic metabolism enzymes
myocardial infarction
NADPH oxidase
physical exercise
rat
ventricular function
description Introduction: Exercise is an important therapeutic strategy for preventing and treating myocardial infarction (MI)-induced cardiac remodeling and heart failure. However, the myocardial effects of resistance exercise on infarcted hearts are not completely established. In this study, we investigated the effects of resistance exercise on structural, functional, and molecular cardiac alterations in infarcted rats. Methods: Three months after MI induction or simulated surgery, Wistar rats were assigned into three groups: Sham (n = 14); MI (n = 9); and exercised MI (MI-Ex, n = 13). Exercised rats performed, 3 times a week for 12 weeks, four climbs on a ladder with progressive loads. Cardiac structure and left ventricle (LV) function were analyzed by echocardiogram. Myocyte diameters were evaluated in hematoxylin- and eosin-stained histological sections as the smallest distance between borders drawn across the nucleus. Myocardial energy metabolism, lipid hydroperoxide, malondialdehyde, protein carbonylation, and antioxidant enzyme activities were evaluated by spectrophotometry. Gene expressions of NADPH oxidase subunits were evaluated by RT-PCR. Statistical analyses were performed using ANOVA and Tukey or Kruskal–Wallis and Dunn’s test. Results: Mortality did not differ between the MI-Ex and MI groups. MI had dilated left atrium and LV, with LV systolic dysfunction. Exercise increased the maximum load-carrying capacity, with no changes in cardiac structure or LV function. Myocyte diameters were lower in MI than in Sham and MI-Ex. Lactate dehydrogenase and creatine kinase activity were lower in MI than in Sham. Citrate synthase and catalase activity were lower in MI and MI-Ex than in Sham. Lipid hydroperoxide concentration was lower in MI-Ex than in MI. Nox2 and p22phox gene expressions were higher in MI-Ex than in Sham. Gene expression of Nox4 was higher in MI and MI-Ex than in Sham, and p47phox was lower in MI than in Sham. Conclusion: Late resistance exercise was safe in infarcted rats. Resistance exercise improved maximum load-carrying capacity, reduced myocardial oxidative stress, and preserved myocardial metabolism, with no changes in cardiac structure or left ventricle function in infarcted rats.
publishDate 2023
dc.date.none.fl_str_mv 2023-07-29T13:53:10Z
2023-07-29T13:53:10Z
2023-04-01
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.3390/antiox12040896
Antioxidants, v. 12, n. 4, 2023.
2076-3921
http://hdl.handle.net/11449/248768
10.3390/antiox12040896
2-s2.0-85156199692
url http://dx.doi.org/10.3390/antiox12040896
http://hdl.handle.net/11449/248768
identifier_str_mv Antioxidants, v. 12, n. 4, 2023.
2076-3921
10.3390/antiox12040896
2-s2.0-85156199692
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Antioxidants
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.none.fl_str_mv Scopus
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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