Rhizobium spp exopolysaccharides production and xanthan lyase use on its structural modification
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.ijbiomac.2019.06.077 http://hdl.handle.net/11449/187760 |
Resumo: | Enzymes can be very useful on exopolysaccharides (EPS) research, can be used at elucidation and also to modify the polysaccharides' structure in order to alter their physical properties. Thus, the reduction of the molecular mass could increase applications of these biopolymers. Therefore, the EPS production of different rhizobia and the action of xanthan lyase on its structures were evaluated. The strains produced significant amounts of EPS, and it was noticed that are heteropolysaccharides, composed galactose and glucose. Both EPS and xanthan were modified on β-glycosidic bonds, the mannose was removed of xanthan had but the EPS was affected in the C[dbnd]O stretching vibration, where the glucuronic acid removed from of your structure. The ester/carboxylic acid portions affected functional groups of the acetate/succinate, methyl carbons of the O-acetyl and pyruvate methyl groups in addition to affect the carbons the main pyranoid. The Resistance to temperature increase of the EPS was observed, made possible by the activity of the lyase. EPS has the ability to form stable gels at higher temperatures and anionic feature can be used on solubilization and controlled release of substances. Modified EPS knowledge will presently facilitate future investigations relating the structure of the rhizobia polysaccharide against rheological properties. |
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Rhizobium spp exopolysaccharides production and xanthan lyase use on its structural modificationInfrared spectroscopyPolysaccharide lyaseRhizobiaEnzymes can be very useful on exopolysaccharides (EPS) research, can be used at elucidation and also to modify the polysaccharides' structure in order to alter their physical properties. Thus, the reduction of the molecular mass could increase applications of these biopolymers. Therefore, the EPS production of different rhizobia and the action of xanthan lyase on its structures were evaluated. The strains produced significant amounts of EPS, and it was noticed that are heteropolysaccharides, composed galactose and glucose. Both EPS and xanthan were modified on β-glycosidic bonds, the mannose was removed of xanthan had but the EPS was affected in the C[dbnd]O stretching vibration, where the glucuronic acid removed from of your structure. The ester/carboxylic acid portions affected functional groups of the acetate/succinate, methyl carbons of the O-acetyl and pyruvate methyl groups in addition to affect the carbons the main pyranoid. The Resistance to temperature increase of the EPS was observed, made possible by the activity of the lyase. EPS has the ability to form stable gels at higher temperatures and anionic feature can be used on solubilization and controlled release of substances. Modified EPS knowledge will presently facilitate future investigations relating the structure of the rhizobia polysaccharide against rheological properties.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)São Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences Department of Technology, Via de Acesso Prof. Paulo Donato Castellane s/nSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences Department of Biology Applied to Agriculture, Via de Acesso Prof. Paulo Donato Castellane s/nSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences Department of Technology, Via de Acesso Prof. Paulo Donato Castellane s/nSão Paulo State University (Unesp) School of Agricultural and Veterinarian Sciences Department of Biology Applied to Agriculture, Via de Acesso Prof. Paulo Donato Castellane s/nCNPq: 401886/2016-1Universidade Estadual Paulista (Unesp)de Sousa, Bruna Fernanda Silva [UNESP]Castellane, Tereza Cristina Luque [UNESP]Campanharo, João Carlos [UNESP]de Macedo Lemos, Eliana Gertrudes [UNESP]2019-10-06T15:46:23Z2019-10-06T15:46:23Z2019-09-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article424-435http://dx.doi.org/10.1016/j.ijbiomac.2019.06.077International Journal of Biological Macromolecules, v. 136, p. 424-435.1879-00030141-8130http://hdl.handle.net/11449/18776010.1016/j.ijbiomac.2019.06.0772-s2.0-85067425462Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengInternational Journal of Biological Macromoleculesinfo:eu-repo/semantics/openAccess2021-10-23T11:59:59Zoai:repositorio.unesp.br:11449/187760Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462021-10-23T11:59:59Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Rhizobium spp exopolysaccharides production and xanthan lyase use on its structural modification |
title |
Rhizobium spp exopolysaccharides production and xanthan lyase use on its structural modification |
spellingShingle |
Rhizobium spp exopolysaccharides production and xanthan lyase use on its structural modification de Sousa, Bruna Fernanda Silva [UNESP] Infrared spectroscopy Polysaccharide lyase Rhizobia |
title_short |
Rhizobium spp exopolysaccharides production and xanthan lyase use on its structural modification |
title_full |
Rhizobium spp exopolysaccharides production and xanthan lyase use on its structural modification |
title_fullStr |
Rhizobium spp exopolysaccharides production and xanthan lyase use on its structural modification |
title_full_unstemmed |
Rhizobium spp exopolysaccharides production and xanthan lyase use on its structural modification |
title_sort |
Rhizobium spp exopolysaccharides production and xanthan lyase use on its structural modification |
author |
de Sousa, Bruna Fernanda Silva [UNESP] |
author_facet |
de Sousa, Bruna Fernanda Silva [UNESP] Castellane, Tereza Cristina Luque [UNESP] Campanharo, João Carlos [UNESP] de Macedo Lemos, Eliana Gertrudes [UNESP] |
author_role |
author |
author2 |
Castellane, Tereza Cristina Luque [UNESP] Campanharo, João Carlos [UNESP] de Macedo Lemos, Eliana Gertrudes [UNESP] |
author2_role |
author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
de Sousa, Bruna Fernanda Silva [UNESP] Castellane, Tereza Cristina Luque [UNESP] Campanharo, João Carlos [UNESP] de Macedo Lemos, Eliana Gertrudes [UNESP] |
dc.subject.por.fl_str_mv |
Infrared spectroscopy Polysaccharide lyase Rhizobia |
topic |
Infrared spectroscopy Polysaccharide lyase Rhizobia |
description |
Enzymes can be very useful on exopolysaccharides (EPS) research, can be used at elucidation and also to modify the polysaccharides' structure in order to alter their physical properties. Thus, the reduction of the molecular mass could increase applications of these biopolymers. Therefore, the EPS production of different rhizobia and the action of xanthan lyase on its structures were evaluated. The strains produced significant amounts of EPS, and it was noticed that are heteropolysaccharides, composed galactose and glucose. Both EPS and xanthan were modified on β-glycosidic bonds, the mannose was removed of xanthan had but the EPS was affected in the C[dbnd]O stretching vibration, where the glucuronic acid removed from of your structure. The ester/carboxylic acid portions affected functional groups of the acetate/succinate, methyl carbons of the O-acetyl and pyruvate methyl groups in addition to affect the carbons the main pyranoid. The Resistance to temperature increase of the EPS was observed, made possible by the activity of the lyase. EPS has the ability to form stable gels at higher temperatures and anionic feature can be used on solubilization and controlled release of substances. Modified EPS knowledge will presently facilitate future investigations relating the structure of the rhizobia polysaccharide against rheological properties. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-10-06T15:46:23Z 2019-10-06T15:46:23Z 2019-09-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.ijbiomac.2019.06.077 International Journal of Biological Macromolecules, v. 136, p. 424-435. 1879-0003 0141-8130 http://hdl.handle.net/11449/187760 10.1016/j.ijbiomac.2019.06.077 2-s2.0-85067425462 |
url |
http://dx.doi.org/10.1016/j.ijbiomac.2019.06.077 http://hdl.handle.net/11449/187760 |
identifier_str_mv |
International Journal of Biological Macromolecules, v. 136, p. 424-435. 1879-0003 0141-8130 10.1016/j.ijbiomac.2019.06.077 2-s2.0-85067425462 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
International Journal of Biological Macromolecules |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
424-435 |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1799964889641385984 |