Radiographic and histological study of perennial bone defect repair in rat calvaria after treatment with blocks of porous bovine organic graft material

Detalhes bibliográficos
Autor(a) principal: Marins, Lucele Vieira
Data de Publicação: 2004
Outros Autores: Cestari, Tania Mary, Sottovia, André Dotto [UNESP], Granjeiro, José Mauro, Taga, Rumio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UNESP
Texto Completo: http://dx.doi.org/10.1590/S1678-77572004000100012
http://hdl.handle.net/11449/211803
Resumo: Over the last few years, various bone graft materials of bovine origin to be used in oromaxillofacial surgeries have entered the market. In the present study, we determined the capacity of a block organic bone graft material (Gen-ox, Baumer SA, Brazil) prepared from bovine cancellous bone to promote the repair of critical size bone injuries in rat calvaria. A transosseous defect measuring approximately 8mm in diameter was performed with a surgical trephine in the parietal bone of 25 rats. In 15 animals, the defects were filled with a block of graft material measuring 8mm in diameter and soaked in the animal's own blood, and in the other 10 animals the defects were only filled with blood clots. The calvariae of rats receiving the material were collected 1, 3 and 6 months after surgery, and those of animals receiving the blood clots were collected immediately and 6 months after surgery. During surgery, the graft material was found to be of easy handling and to adapt perfectly to the receptor bed after soaking in blood. The results showed that, in most animals treated, the material was slowly resorbed and served as a space filling and maintenance material, favoring angiogenesis, cell migration and adhesion, and bone neoformation from the borders of the lesion. However, a foreign body-type granulomatous reaction, with the presence of numerous giant cells preventing local bone neoformation, was observed in two animals of the 1-month subgroup and in one animal of the 3-month subgroup. These cases were interpreted as resulting from the absence of demineralization and the lack of removal of potential antigen factors during production of the biomaterial. We conclude that, with improvement in the quality control of the material production, block organic bone matrix will become a good alternative for bone defect repair in the oromaxillofacial region due to its high osteoconductive capacity.
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spelling Radiographic and histological study of perennial bone defect repair in rat calvaria after treatment with blocks of porous bovine organic graft materialEstudo radiográfico e histológico do reparo de defeito ósseo perene em calvária de rato após o tratamento com material de enxerto orgânico bovino porosoXenogenic graftBovine cancellous boneCritical sizeSkullRatsReparo ósseoOsso medular bovinoDefeito pereneCalváriaRatosOver the last few years, various bone graft materials of bovine origin to be used in oromaxillofacial surgeries have entered the market. In the present study, we determined the capacity of a block organic bone graft material (Gen-ox, Baumer SA, Brazil) prepared from bovine cancellous bone to promote the repair of critical size bone injuries in rat calvaria. A transosseous defect measuring approximately 8mm in diameter was performed with a surgical trephine in the parietal bone of 25 rats. In 15 animals, the defects were filled with a block of graft material measuring 8mm in diameter and soaked in the animal's own blood, and in the other 10 animals the defects were only filled with blood clots. The calvariae of rats receiving the material were collected 1, 3 and 6 months after surgery, and those of animals receiving the blood clots were collected immediately and 6 months after surgery. During surgery, the graft material was found to be of easy handling and to adapt perfectly to the receptor bed after soaking in blood. The results showed that, in most animals treated, the material was slowly resorbed and served as a space filling and maintenance material, favoring angiogenesis, cell migration and adhesion, and bone neoformation from the borders of the lesion. However, a foreign body-type granulomatous reaction, with the presence of numerous giant cells preventing local bone neoformation, was observed in two animals of the 1-month subgroup and in one animal of the 3-month subgroup. These cases were interpreted as resulting from the absence of demineralization and the lack of removal of potential antigen factors during production of the biomaterial. We conclude that, with improvement in the quality control of the material production, block organic bone matrix will become a good alternative for bone defect repair in the oromaxillofacial region due to its high osteoconductive capacity.Nos últimos anos, vários materiais de origem bovina para enxerto ósseo têm sido lançados no comércio para serem utilizados em cirurgias bucomaxilofaciais. Na presente pesquisa avaliamos a capacidade de um material de enxerto ósseo orgânico em bloco (Gen-ox, Baumer SA - Brasil) preparado à partir de osso medular bovino, em promover a reparação de lesões ósseas de tamanho crítico em calvária de ratos. Uma lesão trans-óssea de aproximadamente 8mm de diâmetro foi realizada com trefina cirúrgica nos ossos parietais de 25 ratos, sendo que em 15 os defeitos foram preenchidos com bloco de 8mm de diâmetro do material de enxerto, embebida com sangue do próprio animal e em 10 somente com coágulo sanguíneo. As calvárias dos ratos tratados com o material foram coletadas após 1, 3 e 6 meses após as cirurgias e dos tratados com coágulo sanguíneo, imediatamente após as cirurgias e decorridos 6 meses. Durante as cirurgias verificamos que esse material enxertado é de fácil manuseio e se adapta perfeitamente ao leito receptor após sua embebição com sangue. Os resultados obtidos mostraram que na maioria dos casos tratados, o material foi reabsorvido lentamente e serviu como material de preenchimento e mantenedora de espaço, favorecendo a angiogênese, migração e adesão celular e a neoformação óssea à partir das bordas da lesão. No entanto, em 2 casos no subgrupo tratado - 1 mês e 1 caso no subgrupo tratado - 3 meses, ocorreu a presença de reação granulomatosa tipo corpo estranho com a presença de inúmeras células gigantes, que inibiu a neoformação óssea local. Esses casos foram interpretados como decorrentes de falhas na desmineralização e na retirada de potenciais fatores antigênicos durante a produção do biomaterial. Concluímos que com um aprimoramento do controle de qualidade na linha de produção do material, a matriz orgânica medular em bloco poderá ser uma boa alternativa para ser usada no reparo de defeitos ósseos na região buco-maxilofacial, devido a sua alta capacidade osteocondutora.Universidade Estadual Paulista, Araçatuba Dental SchoolUniversidade de São Paulo, Bauru Dental SchoolUniversidade Estadual Paulista, Araçatuba Dental SchoolFaculdade De Odontologia De Bauru - USPUniversidade Estadual Paulista (Unesp)Universidade de São Paulo (USP)Marins, Lucele VieiraCestari, Tania MarySottovia, André Dotto [UNESP]Granjeiro, José MauroTaga, Rumio2021-07-14T10:29:54Z2021-07-14T10:29:54Z2004-03info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article62-69application/pdfhttp://dx.doi.org/10.1590/S1678-77572004000100012Journal of Applied Oral Science. Bauru, SP, Brazil: Faculdade De Odontologia De Bauru - USP, v. 12, n. 1, p. 62-69, 2004.1678-77571678-7765http://hdl.handle.net/11449/21180310.1590/S1678-77572004000100012S1678-77572004000100012S1678-77572004000100012.pdfSciELOreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengJournal of Applied Oral Scienceinfo:eu-repo/semantics/openAccess2023-10-29T06:06:07Zoai:repositorio.unesp.br:11449/211803Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:19:55.715767Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false
dc.title.none.fl_str_mv Radiographic and histological study of perennial bone defect repair in rat calvaria after treatment with blocks of porous bovine organic graft material
Estudo radiográfico e histológico do reparo de defeito ósseo perene em calvária de rato após o tratamento com material de enxerto orgânico bovino poroso
title Radiographic and histological study of perennial bone defect repair in rat calvaria after treatment with blocks of porous bovine organic graft material
spellingShingle Radiographic and histological study of perennial bone defect repair in rat calvaria after treatment with blocks of porous bovine organic graft material
Marins, Lucele Vieira
Xenogenic graft
Bovine cancellous bone
Critical size
Skull
Rats
Reparo ósseo
Osso medular bovino
Defeito perene
Calvária
Ratos
title_short Radiographic and histological study of perennial bone defect repair in rat calvaria after treatment with blocks of porous bovine organic graft material
title_full Radiographic and histological study of perennial bone defect repair in rat calvaria after treatment with blocks of porous bovine organic graft material
title_fullStr Radiographic and histological study of perennial bone defect repair in rat calvaria after treatment with blocks of porous bovine organic graft material
title_full_unstemmed Radiographic and histological study of perennial bone defect repair in rat calvaria after treatment with blocks of porous bovine organic graft material
title_sort Radiographic and histological study of perennial bone defect repair in rat calvaria after treatment with blocks of porous bovine organic graft material
author Marins, Lucele Vieira
author_facet Marins, Lucele Vieira
Cestari, Tania Mary
Sottovia, André Dotto [UNESP]
Granjeiro, José Mauro
Taga, Rumio
author_role author
author2 Cestari, Tania Mary
Sottovia, André Dotto [UNESP]
Granjeiro, José Mauro
Taga, Rumio
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universidade Estadual Paulista (Unesp)
Universidade de São Paulo (USP)
dc.contributor.author.fl_str_mv Marins, Lucele Vieira
Cestari, Tania Mary
Sottovia, André Dotto [UNESP]
Granjeiro, José Mauro
Taga, Rumio
dc.subject.por.fl_str_mv Xenogenic graft
Bovine cancellous bone
Critical size
Skull
Rats
Reparo ósseo
Osso medular bovino
Defeito perene
Calvária
Ratos
topic Xenogenic graft
Bovine cancellous bone
Critical size
Skull
Rats
Reparo ósseo
Osso medular bovino
Defeito perene
Calvária
Ratos
description Over the last few years, various bone graft materials of bovine origin to be used in oromaxillofacial surgeries have entered the market. In the present study, we determined the capacity of a block organic bone graft material (Gen-ox, Baumer SA, Brazil) prepared from bovine cancellous bone to promote the repair of critical size bone injuries in rat calvaria. A transosseous defect measuring approximately 8mm in diameter was performed with a surgical trephine in the parietal bone of 25 rats. In 15 animals, the defects were filled with a block of graft material measuring 8mm in diameter and soaked in the animal's own blood, and in the other 10 animals the defects were only filled with blood clots. The calvariae of rats receiving the material were collected 1, 3 and 6 months after surgery, and those of animals receiving the blood clots were collected immediately and 6 months after surgery. During surgery, the graft material was found to be of easy handling and to adapt perfectly to the receptor bed after soaking in blood. The results showed that, in most animals treated, the material was slowly resorbed and served as a space filling and maintenance material, favoring angiogenesis, cell migration and adhesion, and bone neoformation from the borders of the lesion. However, a foreign body-type granulomatous reaction, with the presence of numerous giant cells preventing local bone neoformation, was observed in two animals of the 1-month subgroup and in one animal of the 3-month subgroup. These cases were interpreted as resulting from the absence of demineralization and the lack of removal of potential antigen factors during production of the biomaterial. We conclude that, with improvement in the quality control of the material production, block organic bone matrix will become a good alternative for bone defect repair in the oromaxillofacial region due to its high osteoconductive capacity.
publishDate 2004
dc.date.none.fl_str_mv 2004-03
2021-07-14T10:29:54Z
2021-07-14T10:29:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://dx.doi.org/10.1590/S1678-77572004000100012
Journal of Applied Oral Science. Bauru, SP, Brazil: Faculdade De Odontologia De Bauru - USP, v. 12, n. 1, p. 62-69, 2004.
1678-7757
1678-7765
http://hdl.handle.net/11449/211803
10.1590/S1678-77572004000100012
S1678-77572004000100012
S1678-77572004000100012.pdf
url http://dx.doi.org/10.1590/S1678-77572004000100012
http://hdl.handle.net/11449/211803
identifier_str_mv Journal of Applied Oral Science. Bauru, SP, Brazil: Faculdade De Odontologia De Bauru - USP, v. 12, n. 1, p. 62-69, 2004.
1678-7757
1678-7765
10.1590/S1678-77572004000100012
S1678-77572004000100012
S1678-77572004000100012.pdf
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv Journal of Applied Oral Science
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 62-69
application/pdf
dc.publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
publisher.none.fl_str_mv Faculdade De Odontologia De Bauru - USP
dc.source.none.fl_str_mv SciELO
reponame:Repositório Institucional da UNESP
instname:Universidade Estadual Paulista (UNESP)
instacron:UNESP
instname_str Universidade Estadual Paulista (UNESP)
instacron_str UNESP
institution UNESP
reponame_str Repositório Institucional da UNESP
collection Repositório Institucional da UNESP
repository.name.fl_str_mv Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)
repository.mail.fl_str_mv
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