Gene Expression Profile Induced by Two Different Variants of Street Rabies Virus in Mice
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/v14040692 http://hdl.handle.net/11449/223783 |
Resumo: | Pathogenicity and pathology of rabies virus (RABV) varies according to the variant, but the mechanisms are not completely known. In this study, gene expression profile in brains of mice experimentally infected with RABV isolated from a human case of dog rabies (V2) or vampire bat-acquired rabies (V3) were analyzed. In total, 138 array probes associated with 120 genes were expressed differentially between mice inoculated with V2 and sham-inoculated control mice at day 10 post-inoculation. A single probe corresponding to an unannotated gene was identified in V3 versus control mice. Gene ontology (GO) analysis revealed that all of the genes upregulated in mice inoculated with V2 RABV were involved in the biological process of immune defense against pathogens. Although both variants are considered pathogenic, inoculation by the same conditions generated different gene expression results, which is likely due to differences in pathogenesis between the dog and bat RABV variants. This study demonstrated the global gene expression in experimental infection due to V3 wild-type RABV, from the vampire bat Desmodus rotundus, an important source of infection for humans, domestic animals and wildlife in Latin America. |
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Gene Expression Profile Induced by Two Different Variants of Street Rabies Virus in Micedogmicemicroarray analysispathogenesisrabies virusvampire batPathogenicity and pathology of rabies virus (RABV) varies according to the variant, but the mechanisms are not completely known. In this study, gene expression profile in brains of mice experimentally infected with RABV isolated from a human case of dog rabies (V2) or vampire bat-acquired rabies (V3) were analyzed. In total, 138 array probes associated with 120 genes were expressed differentially between mice inoculated with V2 and sham-inoculated control mice at day 10 post-inoculation. A single probe corresponding to an unannotated gene was identified in V3 versus control mice. Gene ontology (GO) analysis revealed that all of the genes upregulated in mice inoculated with V2 RABV were involved in the biological process of immune defense against pathogens. Although both variants are considered pathogenic, inoculation by the same conditions generated different gene expression results, which is likely due to differences in pathogenesis between the dog and bat RABV variants. This study demonstrated the global gene expression in experimental infection due to V3 wild-type RABV, from the vampire bat Desmodus rotundus, an important source of infection for humans, domestic animals and wildlife in Latin America.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Faculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista Julio de Mesquita Filho, Distrito de Rubião Júnior, s/n, CEP, SPSchool of Veterinary Medicine and Science University of Nottingham, Sutton Bonington CampusInternational Research Center A.C Camargo Cancer Center, SPFaculdade de Medicina Veterinária e Zootecnia Universidade Estadual Paulista Julio de Mesquita Filho, Distrito de Rubião Júnior, s/n, CEP, SPFAPESP: 2015/13818-7CNPq: 482726/2012-1Universidade Estadual Paulista (UNESP)University of NottinghamA.C Camargo Cancer CenterAppolinário, Camila M. [UNESP]Daly, Janet M.Emes, Richard D.Marchi, Fabio AlbuquerqueRibeiro, Bruna Leticia Devidé [UNESP]Megid, Jane [UNESP]2022-04-28T19:52:59Z2022-04-28T19:52:59Z2022-04-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/v14040692Viruses, v. 14, n. 4, 2022.1999-4915http://hdl.handle.net/11449/22378310.3390/v140406922-s2.0-85127766835Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengVirusesinfo:eu-repo/semantics/openAccess2022-04-28T19:52:59Zoai:repositorio.unesp.br:11449/223783Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T19:13:33.228153Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Gene Expression Profile Induced by Two Different Variants of Street Rabies Virus in Mice |
title |
Gene Expression Profile Induced by Two Different Variants of Street Rabies Virus in Mice |
spellingShingle |
Gene Expression Profile Induced by Two Different Variants of Street Rabies Virus in Mice Appolinário, Camila M. [UNESP] dog mice microarray analysis pathogenesis rabies virus vampire bat |
title_short |
Gene Expression Profile Induced by Two Different Variants of Street Rabies Virus in Mice |
title_full |
Gene Expression Profile Induced by Two Different Variants of Street Rabies Virus in Mice |
title_fullStr |
Gene Expression Profile Induced by Two Different Variants of Street Rabies Virus in Mice |
title_full_unstemmed |
Gene Expression Profile Induced by Two Different Variants of Street Rabies Virus in Mice |
title_sort |
Gene Expression Profile Induced by Two Different Variants of Street Rabies Virus in Mice |
author |
Appolinário, Camila M. [UNESP] |
author_facet |
Appolinário, Camila M. [UNESP] Daly, Janet M. Emes, Richard D. Marchi, Fabio Albuquerque Ribeiro, Bruna Leticia Devidé [UNESP] Megid, Jane [UNESP] |
author_role |
author |
author2 |
Daly, Janet M. Emes, Richard D. Marchi, Fabio Albuquerque Ribeiro, Bruna Leticia Devidé [UNESP] Megid, Jane [UNESP] |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) University of Nottingham A.C Camargo Cancer Center |
dc.contributor.author.fl_str_mv |
Appolinário, Camila M. [UNESP] Daly, Janet M. Emes, Richard D. Marchi, Fabio Albuquerque Ribeiro, Bruna Leticia Devidé [UNESP] Megid, Jane [UNESP] |
dc.subject.por.fl_str_mv |
dog mice microarray analysis pathogenesis rabies virus vampire bat |
topic |
dog mice microarray analysis pathogenesis rabies virus vampire bat |
description |
Pathogenicity and pathology of rabies virus (RABV) varies according to the variant, but the mechanisms are not completely known. In this study, gene expression profile in brains of mice experimentally infected with RABV isolated from a human case of dog rabies (V2) or vampire bat-acquired rabies (V3) were analyzed. In total, 138 array probes associated with 120 genes were expressed differentially between mice inoculated with V2 and sham-inoculated control mice at day 10 post-inoculation. A single probe corresponding to an unannotated gene was identified in V3 versus control mice. Gene ontology (GO) analysis revealed that all of the genes upregulated in mice inoculated with V2 RABV were involved in the biological process of immune defense against pathogens. Although both variants are considered pathogenic, inoculation by the same conditions generated different gene expression results, which is likely due to differences in pathogenesis between the dog and bat RABV variants. This study demonstrated the global gene expression in experimental infection due to V3 wild-type RABV, from the vampire bat Desmodus rotundus, an important source of infection for humans, domestic animals and wildlife in Latin America. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-04-28T19:52:59Z 2022-04-28T19:52:59Z 2022-04-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/v14040692 Viruses, v. 14, n. 4, 2022. 1999-4915 http://hdl.handle.net/11449/223783 10.3390/v14040692 2-s2.0-85127766835 |
url |
http://dx.doi.org/10.3390/v14040692 http://hdl.handle.net/11449/223783 |
identifier_str_mv |
Viruses, v. 14, n. 4, 2022. 1999-4915 10.3390/v14040692 2-s2.0-85127766835 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Viruses |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808129036581339136 |