Evaluation of short-interfering RNAs treatment in experimental rabies due to wild-type virus
Autor(a) principal: | |
---|---|
Data de Publicação: | 2015 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.1016/j.bjid.2015.05.008 http://hdl.handle.net/11449/131143 |
Resumo: | We have evaluated the efficacy of short-interfering RNAs targeting the nucleoprotein gene and also the brain immune response in treated and non-treated infected mice. Mice were inoculated with wild-type virus, classified as dog (hv2) or vampire bat (hv3) variants and both groups were treated or leaved as controls. No difference was observed in the lethality rate between treated and non-treated groups, although clinical evaluation of hv2 infected mice showed differences in the severity of clinical disease (p=0.0006). Evaluation of brain immune response 5 days post-inoculation in treated hv2 group showed no difference among the analyzed genes, whereas after 10 days post-inoculation there was increased expression of 2',5'-oligoadenylate synthetase 1, tumor necrosis factor alpha, interleukin 12, interferon gamma, and C-X-C motif chemokine 10 associated with higher expression of N gene in the same period (p<0.0001). In hv2 non-treated group only higher interferon beta expression was found at day 5. The observed differences in results of the immune response genes between treated and non-treated groups is not promising as they had neither impact on mortality nor even a reduction in the expression of N gene in siRNA treated animals. This finding suggests that the use of pre-designed siRNA alone may not be useful in rabies treatment. |
id |
UNSP_dc60628c32307ac5d1a0d078fc6d47f3 |
---|---|
oai_identifier_str |
oai:repositorio.unesp.br:11449/131143 |
network_acronym_str |
UNSP |
network_name_str |
Repositório Institucional da UNESP |
repository_id_str |
2946 |
spelling |
Evaluation of short-interfering RNAs treatment in experimental rabies due to wild-type virusBat virusDog virusRabiesSirnaWe have evaluated the efficacy of short-interfering RNAs targeting the nucleoprotein gene and also the brain immune response in treated and non-treated infected mice. Mice were inoculated with wild-type virus, classified as dog (hv2) or vampire bat (hv3) variants and both groups were treated or leaved as controls. No difference was observed in the lethality rate between treated and non-treated groups, although clinical evaluation of hv2 infected mice showed differences in the severity of clinical disease (p=0.0006). Evaluation of brain immune response 5 days post-inoculation in treated hv2 group showed no difference among the analyzed genes, whereas after 10 days post-inoculation there was increased expression of 2',5'-oligoadenylate synthetase 1, tumor necrosis factor alpha, interleukin 12, interferon gamma, and C-X-C motif chemokine 10 associated with higher expression of N gene in the same period (p<0.0001). In hv2 non-treated group only higher interferon beta expression was found at day 5. The observed differences in results of the immune response genes between treated and non-treated groups is not promising as they had neither impact on mortality nor even a reduction in the expression of N gene in siRNA treated animals. This finding suggests that the use of pre-designed siRNA alone may not be useful in rabies treatment.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Universidade Estadual Paulista, Departamento de Higiene Veterinária e Saúde Pública, Faculdade de Medicina Veterinária e Zootecnia de BotucatuFAPESP: 2008/11446-1CNPq: 482726/2012-1Elsevier B. V.Universidade Estadual Paulista (Unesp)Appolinario, Camila Michele [UNESP]Allendorf, Susan Dora [UNESP]Peres, Marina Gea [UNESP]Fonseca, Clovis Reynaldo [UNESP]Vicente, Acacia Ferreira [UNESP]Antunes, João Marcelo Azevedo de Paula [UNESP]Pantoja, José Carlos Figueiredo [UNESP]Megid, Jane [UNESP]2015-12-07T15:31:59Z2015-12-07T15:31:59Z2015info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article453-458application/pdfhttp://dx.doi.org/10.1016/j.bjid.2015.05.008The Brazilian Journal Of Infectious Diseases : An Official Publication Of The Brazilian Society Of Infectious Diseases, v. 19, n. 5, p. 453-458, 2015.1678-4391http://hdl.handle.net/11449/13114310.1016/j.bjid.2015.05.008S1413-86702015000500453S1413-86702015000500453.pdf26254692PubMedreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengThe Brazilian Journal Of Infectious Diseases : An Official Publication Of The Brazilian Society Of Infectious Diseasesinfo:eu-repo/semantics/openAccess2023-11-24T06:14:57Zoai:repositorio.unesp.br:11449/131143Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T18:35:46.166154Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Evaluation of short-interfering RNAs treatment in experimental rabies due to wild-type virus |
title |
Evaluation of short-interfering RNAs treatment in experimental rabies due to wild-type virus |
spellingShingle |
Evaluation of short-interfering RNAs treatment in experimental rabies due to wild-type virus Appolinario, Camila Michele [UNESP] Bat virus Dog virus Rabies Sirna |
title_short |
Evaluation of short-interfering RNAs treatment in experimental rabies due to wild-type virus |
title_full |
Evaluation of short-interfering RNAs treatment in experimental rabies due to wild-type virus |
title_fullStr |
Evaluation of short-interfering RNAs treatment in experimental rabies due to wild-type virus |
title_full_unstemmed |
Evaluation of short-interfering RNAs treatment in experimental rabies due to wild-type virus |
title_sort |
Evaluation of short-interfering RNAs treatment in experimental rabies due to wild-type virus |
author |
Appolinario, Camila Michele [UNESP] |
author_facet |
Appolinario, Camila Michele [UNESP] Allendorf, Susan Dora [UNESP] Peres, Marina Gea [UNESP] Fonseca, Clovis Reynaldo [UNESP] Vicente, Acacia Ferreira [UNESP] Antunes, João Marcelo Azevedo de Paula [UNESP] Pantoja, José Carlos Figueiredo [UNESP] Megid, Jane [UNESP] |
author_role |
author |
author2 |
Allendorf, Susan Dora [UNESP] Peres, Marina Gea [UNESP] Fonseca, Clovis Reynaldo [UNESP] Vicente, Acacia Ferreira [UNESP] Antunes, João Marcelo Azevedo de Paula [UNESP] Pantoja, José Carlos Figueiredo [UNESP] Megid, Jane [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (Unesp) |
dc.contributor.author.fl_str_mv |
Appolinario, Camila Michele [UNESP] Allendorf, Susan Dora [UNESP] Peres, Marina Gea [UNESP] Fonseca, Clovis Reynaldo [UNESP] Vicente, Acacia Ferreira [UNESP] Antunes, João Marcelo Azevedo de Paula [UNESP] Pantoja, José Carlos Figueiredo [UNESP] Megid, Jane [UNESP] |
dc.subject.por.fl_str_mv |
Bat virus Dog virus Rabies Sirna |
topic |
Bat virus Dog virus Rabies Sirna |
description |
We have evaluated the efficacy of short-interfering RNAs targeting the nucleoprotein gene and also the brain immune response in treated and non-treated infected mice. Mice were inoculated with wild-type virus, classified as dog (hv2) or vampire bat (hv3) variants and both groups were treated or leaved as controls. No difference was observed in the lethality rate between treated and non-treated groups, although clinical evaluation of hv2 infected mice showed differences in the severity of clinical disease (p=0.0006). Evaluation of brain immune response 5 days post-inoculation in treated hv2 group showed no difference among the analyzed genes, whereas after 10 days post-inoculation there was increased expression of 2',5'-oligoadenylate synthetase 1, tumor necrosis factor alpha, interleukin 12, interferon gamma, and C-X-C motif chemokine 10 associated with higher expression of N gene in the same period (p<0.0001). In hv2 non-treated group only higher interferon beta expression was found at day 5. The observed differences in results of the immune response genes between treated and non-treated groups is not promising as they had neither impact on mortality nor even a reduction in the expression of N gene in siRNA treated animals. This finding suggests that the use of pre-designed siRNA alone may not be useful in rabies treatment. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-07T15:31:59Z 2015-12-07T15:31:59Z 2015 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.1016/j.bjid.2015.05.008 The Brazilian Journal Of Infectious Diseases : An Official Publication Of The Brazilian Society Of Infectious Diseases, v. 19, n. 5, p. 453-458, 2015. 1678-4391 http://hdl.handle.net/11449/131143 10.1016/j.bjid.2015.05.008 S1413-86702015000500453 S1413-86702015000500453.pdf 26254692 |
url |
http://dx.doi.org/10.1016/j.bjid.2015.05.008 http://hdl.handle.net/11449/131143 |
identifier_str_mv |
The Brazilian Journal Of Infectious Diseases : An Official Publication Of The Brazilian Society Of Infectious Diseases, v. 19, n. 5, p. 453-458, 2015. 1678-4391 10.1016/j.bjid.2015.05.008 S1413-86702015000500453 S1413-86702015000500453.pdf 26254692 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
The Brazilian Journal Of Infectious Diseases : An Official Publication Of The Brazilian Society Of Infectious Diseases |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
453-458 application/pdf |
dc.publisher.none.fl_str_mv |
Elsevier B. V. |
publisher.none.fl_str_mv |
Elsevier B. V. |
dc.source.none.fl_str_mv |
PubMed reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
|
_version_ |
1808128954165362688 |