Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UNESP |
Texto Completo: | http://dx.doi.org/10.3390/molecules28062857 http://hdl.handle.net/11449/249823 |
Resumo: | Candida spp. cause fungal infection that affects patients’ oral health. This study aimed to evaluate the isolated and synergistic antifungal effect of Rosa centifolia L., Curcuma longa L., Rosmarinus officinalis L., and Punica granatum L. glycolic extracts against Candida albicans, Candida dubliniensis, Candida tropicalis, and Candida krusei planktonic and biofilm forms. The plant extracts were chemically characterized and the main compounds were quantified by high-performance liquid chromatography (HPLC-DAD) analysis. The minimum inhibitory and minimum fungicidal concentrations of the extracts were determined, and antibiofilm activity was evaluated by MTT assay. Data were analyzed by one-way ANOVA and Tukey’s tests, and by Kruskal–Wallis and Dunn’s tests, considering a significance level of 5%. The main compounds identified in each of the extracts were: p-coumaric acid (2153.22 μg/100 mL) in the rosemary extract, gallotannins (4318.31 μg/100 mL) in the pomegranate extract, quercetin derivatives (3316.50 μg/100 mL) in the extract of white roses, and curcumin (135.09 μg/100 mL) in the turmeric extract. The combination of R. centifolia and C. longa glycolic extracts was effective against C. albicans, C. dubliniensis, and C. tropicalis biofilms over different periods (p < 0.05). The combination of R. officinalis and P. granatum glycolic extracts was effective against C. albicans and C. krusei biofilms after 30 min, and against C. tropicalis after 24 h, with all combinations showing an average reduction of 50% in cell viability (p < 0.05). In conclusion, the combined plant extracts have antifungal and antibiofilm action against Candida spp. in different concentrations and times of action. |
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Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida SpeciesCandida albicansCandida dubliniensisCandida kruseiCandida tropicalisCurcuma longaPunica granatumRosa centifoliaRosmarinusCandida spp. cause fungal infection that affects patients’ oral health. This study aimed to evaluate the isolated and synergistic antifungal effect of Rosa centifolia L., Curcuma longa L., Rosmarinus officinalis L., and Punica granatum L. glycolic extracts against Candida albicans, Candida dubliniensis, Candida tropicalis, and Candida krusei planktonic and biofilm forms. The plant extracts were chemically characterized and the main compounds were quantified by high-performance liquid chromatography (HPLC-DAD) analysis. The minimum inhibitory and minimum fungicidal concentrations of the extracts were determined, and antibiofilm activity was evaluated by MTT assay. Data were analyzed by one-way ANOVA and Tukey’s tests, and by Kruskal–Wallis and Dunn’s tests, considering a significance level of 5%. The main compounds identified in each of the extracts were: p-coumaric acid (2153.22 μg/100 mL) in the rosemary extract, gallotannins (4318.31 μg/100 mL) in the pomegranate extract, quercetin derivatives (3316.50 μg/100 mL) in the extract of white roses, and curcumin (135.09 μg/100 mL) in the turmeric extract. The combination of R. centifolia and C. longa glycolic extracts was effective against C. albicans, C. dubliniensis, and C. tropicalis biofilms over different periods (p < 0.05). The combination of R. officinalis and P. granatum glycolic extracts was effective against C. albicans and C. krusei biofilms after 30 min, and against C. tropicalis after 24 h, with all combinations showing an average reduction of 50% in cell viability (p < 0.05). In conclusion, the combined plant extracts have antifungal and antibiofilm action against Candida spp. in different concentrations and times of action.Scientific Research and Technology Development Program of GuangxiDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (ICT-UNESP), SPDepartment of Restorative Dentistry Endodontics Division Institute of Science and Technology São Paulo State University (ICT-UNESP), SPDepartment of Biosciences and Oral Diagnosis Institute of Science and Technology São Paulo State University (ICT-UNESP), SPDepartment of Restorative Dentistry Endodontics Division Institute of Science and Technology São Paulo State University (ICT-UNESP), SPScientific Research and Technology Development Program of Guangxi: 5344Universidade Estadual Paulista (UNESP)Meccatti, Vanessa Marques [UNESP]Santos, Lana Ferreira [UNESP]de Carvalho, Lara Steffany [UNESP]Souza, Clara Bulhões [UNESP]Carvalho, Cláudio Antonio Talge [UNESP]Marcucci, Maria Cristina [UNESP]Abu Hasna, Amjad [UNESP]de Oliveira, Luciane Dias [UNESP]2023-07-29T16:10:07Z2023-07-29T16:10:07Z2023-03-01info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articlehttp://dx.doi.org/10.3390/molecules28062857Molecules, v. 28, n. 6, 2023.1420-3049http://hdl.handle.net/11449/24982310.3390/molecules280628572-s2.0-85151648637Scopusreponame:Repositório Institucional da UNESPinstname:Universidade Estadual Paulista (UNESP)instacron:UNESPengMoleculesinfo:eu-repo/semantics/openAccess2023-07-29T16:10:07Zoai:repositorio.unesp.br:11449/249823Repositório InstitucionalPUBhttp://repositorio.unesp.br/oai/requestopendoar:29462024-08-05T16:18:12.693404Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP)false |
dc.title.none.fl_str_mv |
Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species |
title |
Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species |
spellingShingle |
Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species Meccatti, Vanessa Marques [UNESP] Candida albicans Candida dubliniensis Candida krusei Candida tropicalis Curcuma longa Punica granatum Rosa centifolia Rosmarinus |
title_short |
Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species |
title_full |
Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species |
title_fullStr |
Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species |
title_full_unstemmed |
Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species |
title_sort |
Antifungal Action of Herbal Plants’ Glycolic Extracts against Candida Species |
author |
Meccatti, Vanessa Marques [UNESP] |
author_facet |
Meccatti, Vanessa Marques [UNESP] Santos, Lana Ferreira [UNESP] de Carvalho, Lara Steffany [UNESP] Souza, Clara Bulhões [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] Marcucci, Maria Cristina [UNESP] Abu Hasna, Amjad [UNESP] de Oliveira, Luciane Dias [UNESP] |
author_role |
author |
author2 |
Santos, Lana Ferreira [UNESP] de Carvalho, Lara Steffany [UNESP] Souza, Clara Bulhões [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] Marcucci, Maria Cristina [UNESP] Abu Hasna, Amjad [UNESP] de Oliveira, Luciane Dias [UNESP] |
author2_role |
author author author author author author author |
dc.contributor.none.fl_str_mv |
Universidade Estadual Paulista (UNESP) |
dc.contributor.author.fl_str_mv |
Meccatti, Vanessa Marques [UNESP] Santos, Lana Ferreira [UNESP] de Carvalho, Lara Steffany [UNESP] Souza, Clara Bulhões [UNESP] Carvalho, Cláudio Antonio Talge [UNESP] Marcucci, Maria Cristina [UNESP] Abu Hasna, Amjad [UNESP] de Oliveira, Luciane Dias [UNESP] |
dc.subject.por.fl_str_mv |
Candida albicans Candida dubliniensis Candida krusei Candida tropicalis Curcuma longa Punica granatum Rosa centifolia Rosmarinus |
topic |
Candida albicans Candida dubliniensis Candida krusei Candida tropicalis Curcuma longa Punica granatum Rosa centifolia Rosmarinus |
description |
Candida spp. cause fungal infection that affects patients’ oral health. This study aimed to evaluate the isolated and synergistic antifungal effect of Rosa centifolia L., Curcuma longa L., Rosmarinus officinalis L., and Punica granatum L. glycolic extracts against Candida albicans, Candida dubliniensis, Candida tropicalis, and Candida krusei planktonic and biofilm forms. The plant extracts were chemically characterized and the main compounds were quantified by high-performance liquid chromatography (HPLC-DAD) analysis. The minimum inhibitory and minimum fungicidal concentrations of the extracts were determined, and antibiofilm activity was evaluated by MTT assay. Data were analyzed by one-way ANOVA and Tukey’s tests, and by Kruskal–Wallis and Dunn’s tests, considering a significance level of 5%. The main compounds identified in each of the extracts were: p-coumaric acid (2153.22 μg/100 mL) in the rosemary extract, gallotannins (4318.31 μg/100 mL) in the pomegranate extract, quercetin derivatives (3316.50 μg/100 mL) in the extract of white roses, and curcumin (135.09 μg/100 mL) in the turmeric extract. The combination of R. centifolia and C. longa glycolic extracts was effective against C. albicans, C. dubliniensis, and C. tropicalis biofilms over different periods (p < 0.05). The combination of R. officinalis and P. granatum glycolic extracts was effective against C. albicans and C. krusei biofilms after 30 min, and against C. tropicalis after 24 h, with all combinations showing an average reduction of 50% in cell viability (p < 0.05). In conclusion, the combined plant extracts have antifungal and antibiofilm action against Candida spp. in different concentrations and times of action. |
publishDate |
2023 |
dc.date.none.fl_str_mv |
2023-07-29T16:10:07Z 2023-07-29T16:10:07Z 2023-03-01 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://dx.doi.org/10.3390/molecules28062857 Molecules, v. 28, n. 6, 2023. 1420-3049 http://hdl.handle.net/11449/249823 10.3390/molecules28062857 2-s2.0-85151648637 |
url |
http://dx.doi.org/10.3390/molecules28062857 http://hdl.handle.net/11449/249823 |
identifier_str_mv |
Molecules, v. 28, n. 6, 2023. 1420-3049 10.3390/molecules28062857 2-s2.0-85151648637 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
Molecules |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.source.none.fl_str_mv |
Scopus reponame:Repositório Institucional da UNESP instname:Universidade Estadual Paulista (UNESP) instacron:UNESP |
instname_str |
Universidade Estadual Paulista (UNESP) |
instacron_str |
UNESP |
institution |
UNESP |
reponame_str |
Repositório Institucional da UNESP |
collection |
Repositório Institucional da UNESP |
repository.name.fl_str_mv |
Repositório Institucional da UNESP - Universidade Estadual Paulista (UNESP) |
repository.mail.fl_str_mv |
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1808128631348658176 |